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At least 793 records · Page 44Linked to original sources
PubMed1989

Digital flashing tomosynthesis (DFTS)--a technique for three-dimensional coronary angiography.

Digital Flashing Tomosynthesis (DFTS) represents a technique for three-dimensional (3D) coronary angiography. Four ECG-gated simultaneously flashed X-ray tubes generate a multiperspective digital substraction image as DFTS multiangiogram for 3D reconstruction and visualization. Computerized morphologic and morphometric quantitative analysis can be performed including videodensitometry. Postmortem coronary angiography of 30 human hearts with suspected coronary artery disease was performed by 35-mm cine technique and by DFTS. The results of angiographic measurements in 50 stenotic arterial segments were compared with the histologic reference and show excellent regression results with correlation coefficients of more than 0.95 (p less than or equal to 0.0001). No significant differences in standard errors of estimates between the techniques were found. DFTS yields an accuracy in depiction of the coronary arteries and angiographic estimation of arterial lumen equivalent to 35-mm cineangiography. DFTS images can be directly used for visual interpretation and for computerized morphologic and morphometric quantitative analysis. DFTS technology reduces the amount of radiation exposure, the amount of contrast medium, and the time of the procedure. DFTS offers the possibility to obtain 3D images of the coronary artery tree.

Aged↗
PubMed1996

A flash-photolysis study of the reactions of a caa3-type cytochrome oxidase with dioxygen and carbon monoxide.

The time course of absorbance changes following flash photolysis of the fully-reduced carboxycytochrome oxidase from Bacillus PS3 in the presence of O2 has been followed at 445, 550, 605, and 830 nm, and the results have been compared with the corresponding changes in bovine cytochrome oxidase. The PS3 enzyme has a covalently bound cytochrome c subunit and the fully-reduced species therefore accommodates five electrons instead of four as in the bovine enzyme. In the bovine enzyme, following CO dissociation, four phases were observed with time constants of about 10 microseconds, 30 microseconds, 100 microseconds, and I ms at 445 nm. The initial, 10-microsecond absorbance change at 445 nm is similar in the two enzymes. The subsequent phases involving heme a and CuA are not seen in the PS3 enzyme at 445 nm, because these redox centers are re-reduced by the covalently bound cytochrome c, as indicated by absorbance changes at 550 nm. A reaction scheme consistent with the experimental observations is presented. In addition, internal electron-transfer reactions in the absence of O2 were studied following flash-induced CO dissociation from the mixed-valence enzyme. Comparisons of the CO recombination rates in the mixed-valence and fully-reduced oxidases indicate that more electrons were transferred from heme a3 to a in PS3 oxidase compared to the bovine enzyme.

Animals↗
PubMed1996

Biochemical systematics of Japanese fireflies of the subfamily Luciolinae and their flash communication systems.

Japanese fireflies of the subfamily Luciolinae are biochemically analyzed using 13 allozymes, and the phylogenetic relationships obtained from this analysis are compared with their flash communication systems. As a result, the Japanese Luciolinae can be divided into three groups. Hotaria parvula and H. tsushimana together with Luciola yayeyamana and L. kuroiwae form the first group, and they use the same communication system. L. lateralis, Curtos okinawana, and C. costipennis make up the second group, and their communication systems are also the same. L. cruciata makes up the last one, and its communication system is different from the other fireflies of Luciolinae. Therefore, their taxonomical arrangement and communication systems are not congruent. However, the genetic similarity deduced by allozymic analysis of the members of the Japanese Luciolinae is highly consistent with their flash communication systems.

Animal Communication↗
PubMed1992

Potential of digital flashing tomosynthesis for angiocardiographic evaluation.

A system for digital flashing tomosynthesis (DFTS) consists of four electrocardiogram-gated and simultaneously flashed x-ray tubes, a 14" image intensifier, a unit for digital subtraction angiography (DSA), a personal computer-hosted transputer network for three-dimensional (3D) reconstruction as well as for quantitative coronary angiography and ventriculography, a display unit and an individual digital archive. DFTS-tomoangiograms may be presented in single slice mode or as multiple slices of arbitrary thickness, using rotating and stereographic presentation of 3D images. DFTS represents a configuration for standardized digital angiocardiography with digital archiving and assures optimal reproducibility and safety. This angiographic configuration is feasible for both ambulatory angiography to allow high volume cardiovascular angiographic screening and for the quantitative assessment of natural progression or potential regression of coronary artery disease resulting from interventional or pharmacological therapy.

Angiocardiography↗
PubMed2001

On-line flash thermodesorption-GC-MS determination of PCB in sewage sludge.

A flash thermodesorption-gas chromatography-mass spectrometry technique has been developed for the determination of polychlorobiphenyls (PCB), especially the highly toxic planar species, in sewage sludge. A modified Curie-point pyrolyzer was used; in this the flash-desorption temperature was controlled by selecting specific metal alloys for the pyrolysis wire. Samples (3 mg) were loaded into a quartz tube with the special heating wire wound around the outer wall. By this means rapid thermal desorption (TDS) was achieved whereas uncontrolled catalytic breakdown of the analytes was avoided. The thermodesorption unit was connected via a custom-built interface to the GC-MS detection system. The average limit of detection was 15 microgram kg(-1) sludge more than one order of magnitude better than the legal requirement.

Gas Chromatography-Mass Spectrometry↗
PubMed1998

Evaluation of patellar cartilage surface lesions: comparison of CT arthrography and fat-suppressed FLASH 3D MR imaging.

The aim of this study was to evaluate the sensitivity and specificity of fat-suppressed fast low-angle shot (FLASH) 3D MR imaging in the detection of patellar cartilage surface lesions in comparison with CT arthrography. Fifty patients, with or without symptoms of chondromalacia, were prospectively examined by CT arthrography and fat-suppressed 3D gradient-echo MR imaging. All MR examinations were evaluated by three observers, two of them reaching a consensus interpretation. The lesions were graded according to their morphology and their extent. The CT arthrography was considered as the reference examination. For both sets of observers, the final diagnosis of chondromalacia was obtained in 92.5 %. The specificity was 60 % on a patient-by-patient basis. Fissures were missed in 83 and 60 %, respectively, but were isolated findings only in 2.5 % of the cases. Considering ulcers involving more than 50 % of the cartilage thickness, 65 and 88 %, respectively, were recognized. Fat-suppressed FLASH 3D is an adequate pulse sequence for the detection of patellar cartilage ulcers. It can be applied on a routine clinical basis, but it does not show as many fissures as CT arthrography and is less precise for grading of lesions.

Adolescent↗
PubMed1997

Kinetic properties of DM-nitrophen and calcium indicators: rapid transient response to flash photolysis.

We describe a high temporal resolution confocal spot microfluorimetry setup which makes possible the detection of fluorescence transients elicited by Ca2+ indicators in response to large (50-200 microM), short duration (< 100 ns), free [Ca2+] transients generated by laser flash photolysis of DM-nitrophen (DM-n; caged Ca2+). The equilibrium and kinetic properties of the commercially available indicators Fluo-3, Rhod-2, CalciumOrange-5N (COr-5N) and CalciumGreen-2 (CGr-2) were determined experimentally. The data reveal that COr-5N displays simple, fast response kinetics while, in contrast, Fluo-3, Rhod-2 and CGr-2 are characterized by significantly slower kinetic properties. These latter indicators may be unsuitable for tracking Ca2+ signaling events lasting only a few milliseconds. A model which accurately predicts the time course of fluorescence transients in response to rapid free [Ca2+] changes was developed. Experimental data and model predictions concur only when the association rate constant of DM-n is approximately 20 times faster than previously reported. This work establishes a quantitative theoretical framework for the study of fast Ca2+ signaling events and the use of flash photolysis in cells and model systems.

Acetates↗
PubMed2006

The photopic negative response of the flash electroretinogram in retinal vein occlusion.

The photopic negative response (PhNR) has recently been shown to be severely affected in central retinal artery occlusion (CRAO), despite relative preservation of the cone b-wave compared to that in the healthy unaffected fellow eye. The aim of this study was to test how the PhNR of the flash electroretinogram (ERG) is affected in human retinal vein occlusion. PhNR was elicited with red stimuli (1 cd s/m2, 5 cd s/m2, and 7 cd s/m2 with 4 ms duration) and blue background (10 cd/m2). Standard Ganzfeld flash ERG was produced according to the ISCEV standard for the clinical electroretinogram (2004). Sixteen patients with central retinal vein occlusion (CRVO), 14 patients with branch retinal vein occlusion (BRVO), and 16 controls were analyzed. The amplitude of the PhNRs was significantly smaller in the CRVO and BRVO eyes than those in the unaffected fellow or control eyes (p = 0.000). There was a significantly greater reduction of PhNR amplitudes than that of other waves including the OPs, rod b-wave, combined a-wave and b-wave, cone a-wave and b-wave, and 30 Hz flicker ERG. Thus, PhNR amplitude in retinal vein occlusion is severely affected. There is a potential role for PhNR in assessing inner retinal damage and evaluating the effect of treatment.

Adult↗
PubMed2005

Characteristics of cytochrome oxidase activity in visual system neurons in kittens reared in conditions of flashing illumination.

The studies reported here addressed the effects of flashing (15 Hz) lights on the metabolic activity of visual system neurons in animals reared in condition of crepuscular illumination. Activity of the respiratory enzyme cytochrome oxidase was detected in the cortex of visual areas 17 and 18 and in the lateral geniculate body in kittens. The results showed that kittens subjected to this stimulation, unlike intact kittens and kittens reared in conditions of crepuscular illumination, showed a change in the pattern of cytochrome oxidase distribution in cortical field 17 consisting of the appearance of alternating areas of increased and decreased enzyme activity in layers III and IV. In cortical field 18 and the lateral geniculate body, experimental kittens showed no changes in the cytochrome oxidase activity distribution pattern. It is suggested that flashing illumination leads to disturbance of the balance in activity in the Y and X conducting channels of the visual system.

Animals↗
PubMed1992

The influence of signs prompting motorists to yield before marked crosswalks on motor vehicle-pedestrian conflicts at crosswalks with flashing amber.

The purpose of this experiment was to evaluate the effects of signs reading "STOP HERE FOR PEDESTRIANS" alone and in conjunction with advance stop lines on pedestrian safety at multilane crosswalks with pedestrian-activated amber flashing lights. Motorist and pedestrian behaviors measured throughout this experiment included the occurrence of various types of motor vehicle-pedestrian conflicts; the distance that motorists stopped before the crosswalk when yielding to pedestrians; and the percentage of motorists yielding to pedestrians. The introduction of the sign alone 50 feet (15.15 m) before the crosswalk increased the distance before the crosswalk that motorists yielded to pedestrians and reduced the percentage of motor vehicle-pedestrian conflicts whether the flashing light was activated or not. The addition of the advance stop line was associated with further improvements in both measures.

Accidents, Traffic↗
PubMed1975

Bright-flash electroretinography for the evaluation of eyes with opaque vitreous.

Standard retinal function tests are of limited value in assessing retinal function in eyes with opaque vitreous. We developed a method for obtaining an electroretinogram (ERG) from eyes with significant vitreous opacity by utilizing a much brighter than normal stimulating light. Of 115 eyes with vitreous opacities and good ERG responses to this bright-flash photostimulator, 47% would have been nonrecordable with a conventional ERG light source. Bright-flash ERG was often helpful in evaluating eyes with vitreous opacities for vitrectomy and was sometimes the only source of information regarding potential retinal function.

Aged↗
PubMed1977

The effect of synchronization on the interpretation of flashing lamp displays.

The question of whether all the lamps on a display indicating the same state should flash in phase with each other or independently is of interest to display designers. A randomized groups experiment was carried out to answer this question. It was found that a synchronized display was interpreted more quickly and with fewer errors than a display with independently flashing lamps.

Journal Article↗
PubMed1983

Flash spectroscopic characterization of photosynthetic electron transport in isolated heterocysts.

Electron transport was studied in heterocysts of the filamentous cyanobacterium Anabaena 7120 using spectral and kinetic analysis of absorbance transients elicited by single turnover flashes. Consistent photosynthetic turnovers were observed only in the presence of an exogenous source of reductant; therefore measurements were routinely made under a gas phase containing H2. Prominent absorbance changes corresponding to the oxidation of cytochrome c (554 nm) and the reduction of cytochrome b563 (563 nm) were observed. Under the most reducing conditions (99% H2/1% O2) cytochrome b563 was partially reduced between flashes in a slow, dark reaction. At 10-15% O2, the slow, dark reduction of cytochrome b563 was eliminated. Cytochrome turnover ceased entirely at high O2 concentrations (30%) but was restored by the addition of 25 microM KCN, demonstrating an interaction between the photosynthetic and respiratory electron transfer chains. Strobilurin A slowed the re-reduction of cytochrome c and eliminated the appearance of reduced cytochrome b563 by blocking electron transfer between reduced plastoquinone and the cytochrome b/f complex. Inhibition at a second site was apparent with 2-(n-heptyl)-4-hydroxyquinoline N-oxide, which blocked the reoxidation of cytochrome b563 but had little effect on cytochrome c relaxation. In uncoupled heterocysts, the rates of cytochrome c re-reduction and cytochrome b563 reduction were equal. Additional unassigned absorbance changes at 475 nm, 515 nm, and 572 nm were partially characterized. No absorbance change corresponding to an electrochromic shift was observed.

Cyanobacteria↗
PubMed1985

Reconciliation of the absorption change at 325 nm and other flash-yield determinations of concentrations of active photosystem II centers.

The concentration of photosystem II was determined in thylakoids of dwarf peas by the use of the following methods: absorption change at 325 nm; atrazine binding; and flash yields of oxygen evolution (Emerson-Arnold method), of protons from oxidation of water, and of reduction of DCIP. For the first time all of the flash-yield measurements have been done on the same sample and give equivalent values for the concentration of photosystem II. Agreement of the absorption change measurement at 325 nm with the other measurements was accomplished by the introduction of important improvements to the methods of Melis and co-workers [Proc. Natl. Acad. Sci. USA (1980) 77, 4712-4716]. The atrazine-binding method gave photosystem II values that were twice as large as any of the other photosystem II measurements. Possible reasons are discussed for this discrepancy in terms of the secondary acceptor (Q400) of Ikegami and Katoh [Plant Cell Physiol. (1973) 14, 829-836]. The concentration of photosystem I was measured by absorption change at 705 nm. From the concentration values of photosystem II and I the system II/I stoichiometry was calculated.

Atrazine↗
PubMed1975

Delayed fluorescence from Rhodopseudomonas viridis following single flashes.

Delayed fluorescence from Rhodopseudomonas viridis membrane fragments has been studies using a phosphoroscope employing single, short actinic flashes, under conditions of controlled redox potential and temperature. The emission spectrum shows that delayed fluorescence is emitted by the bulk, antenna bacteriochlorophyll. The energy for delayed fluorescence, however, must be stored in a reaction-center complex including the photooxidized form (P+) of the primary electron-donor (P) and the photoreduced form (X MINUS) of the primary electron-acceptor. This is shown by the following observations: (1) Delayed luminescence is quenched (a) at low redox potentials which allow cytochromes to reduce P+ rapidly after the flash, (b) at higher redox potentials which, by oxidizing P chemically, prevent the photochemical formation of P+X minus, and (c) upon transfer of an electron from X minus to a secondary acceptor, Y. (2) Under conditions that prevent the reduction of P+ by cytochromes and the oxidation of X minus by Y, the decay kinetics of delayed fluorescence are identical with those of P+X minus, as measured from optical absorbance changes. The main decay route for P+X minus under these conditions has a rate-constant of approximately 10-3-s-minus 1. In contrase, a comparison of the intensities of delayed and prompt fluorescence indicates that the process in which P+X minus returns energy to the bulk bacteriochlorophyll has a rate-constant of 3.7 s-minus 1, at 295 degrees K and pH 7.8. The decay kinetics of P+X minus and delayed fluorescence change little with temperature, whereas the intensity of delayed fluorescence increases with increasing temperature, having an activation energy of 12.5 kcal mol-mol- minus 1. We conclude that the main decay route involves tunneling of an electron from X minus to P+, without the promotion of P to an excited state. Delayed fluorescence requires such a promotion, followed by transfer of energy to the bulk bacteriochlorophyll, and this combination of events is rare. The activation energy, taken with potentiometric data, indicates that the photochemical conversion of PX to P+X minus results in increases of both the energy and the entropy of the system, by 16.6 kcal-mol- minus 1 and 8.8 cal-mol- minus 1-deg- minus 1. The intensity of delayed fluorescence depends strongly on the pH; the origin of this effect remains unclear.

Calorimetry↗
PubMed1976

Multiple-flash development of thermoluminescence bands in dark-grown spruce leaves.

Spruce leaves greened in darkness were devoid of three of the five thermoluminescence bands found for mature leaves. These bands were developed rapidly by exposure of the dark-grown leaves to continuous light. The development of these bands was studied by illumination with repetitive flashes at varied intervals. Flashes at intervals of 1 s were the most effective in inducing these bands. Those at shorter or longer intervals were less effective. It was deduced from these data that the development of these bands is a multiquantum process which involves at least two photo-events with a dark reaction between them.

Chloroplasts↗
PubMed1976

Energy transfer in the photochemical apparatus of flashed bean leaves.

Fluorescence and energy transfer properties of bean leaves greened by brief, repetitive xenon flashes were studied at -196 degrees C. The bleaching of P-700 has no influence on the yield of fluorescence at any wavelength of emission. The light-induced fluorescence yield changes which are observed in both the 690 and 730 nm emission bands in the low temperature fluorescence spectra are due to changes in the state of the Photosystem II reaction centers. The fluorescence yield changes in the 730 nm band are attributed to energy transfer from Photosystem II to Photosystem I. Such energy transfer was also confirmed by measurements of the rate of photooxidation of P-700 AT -196 degrees C in leaves in which the Photosystem II reaction centers were either all open or all closed. It is concluded that energy transfer from Photosystem II to Photosystem I occurs in the flashed bean leaves which lack the light-harvesting chlorophyll a/b protein.

Cytochromes↗
PubMed1977

515 nm Absorption changes in Chlorella at short times (4--100 mus) after a flash.

Using Chlorella, three types of absorption changes at 515 nm have been studied in the 4-100 mus time range following a flash. (1) The absorption change observed when both photoreactions are blocked, probably due to the formation of the triplet state of a carotenoid, is show to depend on Photosystem II excitation only. (2) The absorption increase induced by photoreaction I is biphasic; first phase, complete in less than 4 mus, followed by a slower phase with a half-rise time of 15-20 mus. (3) On the other hand, photoreaction II induces only a fast absorption increase (lessthan 4 mus). The time course of the biphasic 515 nm absorption increase induced by photoreaction I is similar to the biphasic absorption decrease previously observed at 480 nm by Cox and Delosme (1976, C.R. Acad. Sci. Paris 282D, 775-778). No significant absorption change is observed at 490 nm. These results suggest that the transmembrane electric field induced by photoreaction I rises to its maximum value in at least two phases within 100 mus following flash excitation.

Chlorella↗