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At least 793 records · Page 44Linked to original sources
PubMed1978

Quantitative determination of 1,3-bis(tetrahydro-2- furanyl)-5-fluoro-2,4-pyrimidinedione and its metabolites in plasma by high-pressure liquid chromatography and gas chromatography-mass fragmentography.

1,3-Bis(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione has been developed clinically as an antitumor agent. A high-pressure liquid chromatographic method was developed with which it could be measured in plasma with a sensitivity of 0.050 microgram/ml. Two of its metabolites, 1-(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione and 3-(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione, could be determined at the same time with a sensitivity of 0.025 microgram/ml. A gas chromatographic-mass fragmentographic method was developed for the specific determination of the third metabolite, 5-fluoro-2,4-pyrimidinedione, as its silylated derivative with a sensitivity of 0.001 microgram/ml. The precision and sensitivity of the assay appear to be satisfactory for determination of the plasma level of the drug.

Antineoplastic Agents↗
PubMed1990

High-performance liquid chromatography of amino acids, peptides and proteins. CIII. Mass transfer resistances in ion-exchange and dye-affinity chromatography of proteins.

Adsorption equilibria and rate kinetics have been investigated for the binding of several proteins, with different molecular geometries, to several ion-exchange and dye-affinity chromatographic resins with varying pore size and protein accessibilities. The pore geometry was shown to play a significant role in the protein capacity and loadability of both the ion-exchange and dye-affinity resins. For example the Fractogel HW75-Cibacron Blue F3GA affinity sorbent had the greatest capacity for the small protein, lysozyme, compared to the other Fractogel HW-Cibacron Blue F3GA sorbents, and similarly, the ion-exchange resins, such as DEAE-Fractogel 65, bound more human serum albumin (HSA), as opposed to the larger protein, ferritin. The apparent diffusion of protein from the bulk phase to the ligands/ionic sites was calculated to be considerably restricted when the pore to protein size ratio was small, as is the case of DEAE Fractogel 65/ferritin system, and the dye-affinity Fractogel HW55/HSA system. In these circumstances, pore diffusivity was calculated to be up to 100-fold smaller than bulk diffusivity.

Adsorption↗
PubMed1983

High-performance liquid chromatography of amino acids, peptides and proteins. XLVII. Analytical and semi-preparative separation of several pituitary proteins by high-performance ion-exchange chromatography.

The chromatographic separation of several pituitary proteins on a Mono-Q anion-exchange column is described. The effect of eluent pH and buffer composition on the resolution is demonstrated with several standard proteins. The experimental data indicate that good protein recoveries and resolution can be obtained on this essentially monodisperse microparticulate ion-exchange resin when the pH of the eluent is chosen ca. 0.5 pH units below the pI of the most basic component in the mixture. With this new column separations are ca. 30 times faster than with conventional cellulosic anion exchangers at similar sample loads.

Animals↗