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At least 775 records · Page 43Linked to original sources
PubMed1984

Application of a bonded-phase extraction column for rapid sample preparation of flecainide from human plasma for high-performance liquid chromatographic analysis--fluorescence or ultraviolet detection.

A simple, rapid, selective, and sensitive procedure for the monitoring of flecainide levels in human plasma is described. The drug and the internal standard--a positional isomer of flecainide--are separated from plasma by the use of a disposable extraction column packed with reversed-phase sorbent. The drug and internal standard which are selectively retained on the extraction column are eluted. An aliquot is injected onto a mu-Bondapak phenyl column and eluted with a mixture of acetonitrile and 0.06% phosphoric acid (40:60, vol/vol) at a flow rate of 2 ml/min. The eluted drug and internal standard are measured by a fluorescence detector with excitation and emission wavelengths of 300 and 370 nm, respectively. The linear range is 3-2,000 ng/ml, and the sensitivity limit is 3 ng/ml with 1 ml of plasma. The within-day coefficient of variation was 1.1-4.6% at concentrations ranging from 3 to 1,600 ng/ml. Many drugs given concomitantly with flecainide acetate do not interfere with the assay. The method compares favorably to a well-documented gas-liquid chromatographic method and is suitable for use in plasma drug level monitoring for patient management as well as in pharmacokinetic studies. Similar results were obtained using an alternate detection method (ultraviolet detection at 298 nm).

Chromatography, High Pressure Liquid↗
PubMed1995

Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid.

A PCR assay for the detection of Haemophilus ducreyi in clinical specimens taken from genital ulcers was developed. Although H. ducreyi, when present in such specimens, could be detected by PCR, the sensitivity of the assay was reduced by the presence of Taq polymerase inhibitors in the specimen. The sensitivity of the PCR assay was improved by the use of detergents in preparing nuclei acids from clinical specimens and by the inclusion of a dialysis step prior to amplification. In addition, sodium phosphate included in the transport medium was found to be an inhibitor of the Taq polymerase.

Bacteriological Techniques↗
PubMedDate not supplied

Specimen adequacy of ThinPrep sample preparations in a direct-to-vial study.

OBJECTIVE: To assess specimen adequacy of the ThinPrep slide preparation method in routine use. STUDY DESIGN: Two studies, a feasibility study of 299 women and a clinical study of 499 women, were conducted. A broom-type collection device was used and rinsed directly into Pre-servCyt vials. Slides were prepared with the ThinPrep 2000 device, screened and classified according to the Bethesda System. The proportion of ThinPrep slides described as "Satisfactory But Limited By: No Endocervical Component (SBLB:No ECC)" was then compared to the proportion of SBLB: No ECC slides found on conventional smears in a previously conducted clinical trial of over 7,000 patients. RESULTS: For the feasibility study the proportion of ThinPrep slides described as SBLB: No ECC was 9.36% as compared to the clinical trial combined rate of 9.4% for conventional smears. For the clinical study, 4.96% of ThinPrep slides were SBLB:No ECC as compared to the 4.4% SBLB:No ECC rate for conventional smears from the same clinical trial. The proportions were statistically equivalent for both studies. CONCLUSION: It is expected that the rate of representing endocervical component will be maintained when the ThinPrep preparation method is used routinely in place of the conventional cytologic smear method.

Adult↗