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PubMed2003

Side-effects of three pesticides on the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae).

Side-effects of three commonly used pesticides in Iran were evaluated on an introduced strain of the predatory mite. Phytoseiulus persimilis Athias-Henriot, reared for about 10 years without exposure to any pesticides. Application of pesticides was carried out either to detached bean leaves using a Potter tower at 1 mg wet deposit per cm2 or by a hand sprayer on bean plants until run off. According to an EPPO decision making scheme, pirimiphos-methyl was found to be harmful (E=90.8%) and heptenophos harmless (E=-3.7%) to the predatory mite in the residual initial toxicity tests. For determination of the hazard class of malathion a field test was found to be necessary (E=59.8%). Categories of 1, 2, 3 were determined for heptenophos, malathion and primiphos-methyl, respectively, using IOBC classification. Despite being harmful, it is possible to use pirimiphos-methyl 10 days before release of P. persimilis. Investigation of the contribution of both lethal and sub-lethal effects to total impact indicated the dominance of lethality in the case of pirimiphos-methyl, while malathion acted by both mechanisms. Heptenophos did not have negative effects on fecundity of P. persimilis but rather caused a higher rate of fecundity in comparison with the control. The mortality found in the heptenophos test was not significantly different from the control.

Analysis of Variance↗
PubMed1983

Inhibition of neoplasia by minor dietary constituents.

Food contains a large number of inhibitors of carcinogenesis, including phenols, indoles, aromatic isothiocyanates, methylated flavones, coumarins, plant sterols, selenium salts, protease inhibitors, ascorbic acid, tocopherols, retinol, and carotenes. The diversity and widespread occurrence of these compounds in food make it virtually impossible to consume a diet that does not contain inhibitors of carcinogenesis. Inhibitors can be classified as to the time in the carcinogenic process at which they act. Some prevent formation of carcinogens. Others, termed "blocking agents," prevent carcinogens from reaching or reacting with critical target sites. A third group called "suppressing agents" are effective when fed subsequent to administration of carcinogens. Some compounds inhibit at more than one time point. The major emphasis in this paper is on blocking agents, in particular those that act by enhancing host detoxification systems. Mary blocking agents produce a coordinated enhancement of multiple detoxification systems. Two distinctive patterns termed type A and type B have been identified. One enzyme system commonly induced by blocking agents is glutathione S-transferase. On the basis of this information, induction of glutathione S-transferase activity is being used to detect the presence of blocking agents in complex natural products. Green coffee beans induce increased glutathione S-transferase activity and inhibit mammary neoplasia in the rat resulting from administration of 7,12-dimethylbenz(a)anthracene. Two potent inducers of increased glutathione S-transferase activity have been isolated from green coffee beans. These are kahweol palmitate and cafestol palmitate. In recent work, several plant materials have been found to inhibit carcinogenesis when fed after carcinogen exposure. The identification and further investigation of inhibitors present in food are of importance so that their impact on the occurrence of neoplasia in humans can be ascertained.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1990

Analysis of the structure of a common cold virus, human rhinovirus 14, refined at a resolution of 3.0 A.

Human rhinovirus 14 has a pseudo T = 3 icosahedral structure in which 60 copies of the three larger capsid proteins VP1, VP2 and VP3 are arranged in an icosahedral surface lattice, reminiscent of T = 3 viruses such as tomato bushy stunt virus and southern bean mosaic virus. The overall secondary and tertiary structures of VP1, VP2 and VP3 are very similar. The structure of human rhinovirus 14, which was refined at a resolution of 3.0 A [R = 0.16 for reflections with F greater than 3 sigma(F)], is here analyzed in detail. Quantitative analysis of the surface areas of contact (proportional to hydrophobic free energy of association) supports the previously assigned arrangement within the promoter, in which interactions between VP1 and VP3 predominate. Major contacts among VP1, VP2 and VP3 are between the beta-barrel moieties. VP4 is associated with the capsid interior by a distributed network of contacts with VP1, VP2 and VP3 within a promoter. As the virion assembly proceeds, the solvent-accessible surface area becomes increasingly hydrophilic in character. A mixed parallel and antiparallel seven-stranded sheet is composed of the beta C, beta H, beta E and beta F strands of VP3 in one pentamer and beta A1 and beta A2 of VP2 and the VP1 amino terminus in another pentamer. This association plays an essential role in holding pentamers together in the mature virion as this contact region includes more than half of the total short non-bonded contacts between pentamers. Contacts between protomers within pentamers are more extensive than the contacts between pentamers, accounting in part for the stability of pentamers. The previously identified immunogenic regions are correlated with high solvent accessibility, accessibility to large probes and also high thermal parameters. Surface residues in the canyon, the putative cellular receptor recognition site, have lower thermal parameters than other portions of the human rhinovirus 14 surface. Many of the water molecules in the ordered solvent model are located at subunit interfaces. A number of unusual crevices exist in the protein shell of human rhinovirus 14, including the hydrophobic pocket in VP1 which is the locus of binding for the WIN antiviral agents. These may be required for conformational flexibility during assembly and disassembly. The structures of the beta-barrels of human rhinovirus 14 VP1, VP2 and VP3 are compared with each other and with the southern bean mosaic virus coat protein.

Amino Acid Sequence↗
PubMed1980

Myo-inositol content of common foods: development of a high-myo-inositol diet.

Since virtually no information is available concerning the myo-inositol content of dietary constituents, we have measured the amount of this material present in 487 foods by gas-liquid chromatography. We observed that the greatest amounts of myo-inositol were present in fruits, beans, grains, and nuts. Fresh vegetables and fruits were found to contain more myo-inositol than did frozen, canned, or salt-free products. The data provided in this report were used to develop diets that contained varying, but known amounts of myo-inositol. The myo-inositol intake that could be provided by such diets ranged from 225 to 1500 mg/day per 1800 kcal and within this range the agreement between the calculated and measured amounts of this material was excellent (r = 0.98). Since abnormalities in the metabolism of myo-inositol have been speculated to play a role in the pathogenesis of the polyneuropathies associated with diabetes mellitus and chronic renal failure, it is possible that the natural history of these neuropahties can be altered by modifying the amount of dietary myo-inositol that is ingested by patients with these diseases.

Bread↗
PubMed1992

Variable intron content of the NADH dehydrogenase subunit 4 gene of plant mitochondria.

The gene nad4, encoding subunit four of the mitochondrial NADH dehydrogenase complex I, has been isolated and characterized from turnip, Brassica campestris. The 8 kb turnip nad4 gene contains four exons, which potentially encode a NAD4 polypeptide of 495 amino acids, and three large group II introns. Northern analysis identifies an abundant 2 kb transcript that most likely serves as the nad4 mRNA, while several larger transcripts (putative splicing intermediates) are also detected. Analysis of the nad4 locus in three distantly related dicotyledons indicates that introns 2 and 3 are optional. Mung bean has the same nad4 organization as turnip, whereas spinach nad4 contains introns 1 and 3, and lettuce nad4 has intron 1 only. We infer that all three group II introns were present in the nad4 gene of an angiosperm common ancestor and have persisted in certain lineages for over 200 million years, with two of the introns having been lost in other lineages.

Amino Acid Sequence↗
PubMed1996

Expression of the major bean proteins from Theobroma cacao (cocoa) in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae.

The production in two yeast expression systems of recombinant forms of the major proteins from the cocoa bean is described. Three major protein species are found in the cocoa bean: an albumin of molecular mass 21 kDa (p21) and two insoluble vicilin-like proteins of molecular mass 31 kDa and 47 kDa (p31 and p47, respectively). The p31 and p47 species are known to be derived from a common 67-kDa precursor (p67) by post-translational processing that includes the deletion of a hydrophilic domain located immediately after an N-terminal signal sequence. All three proteins appear to be targeted to membrane-bound storage organelles by N-terminal signal sequences. The p21 and p67 coding sequences were expressed in Hansenula polymorpha using the powerful methanol oxidase (MOX) promoter and in Saccharomyces cerevisiae using the promoter of the pyruvate kinase (PYK) gene. The expression constructs contained the native plant signal sequence, or various yeast signals. The p21 protein was successfully expressed and secreted from both yeasts. The insoluble p67 protein proved more difficult. Species of the correct molecular mass were recovered internally and small amounts of a p47 species were secreted using a yeast leader sequence. However, proteolytic cleavage, probably due to Kex2p-like processing, led to the appearance of other protein species.

Amino Acid Sequence↗
PubMed2004

In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans.

Seven kinds of hydroxycinnamic acid derivatives identified as 3-caffeoylquinic acid (3-CQA), 4-caffeolyquinic acid (4-CQA), 5-caffeoylquinic acid (5-CQA), 5-feruloylquinic acid (5-FQA), 3,4-dicaffeoylquinic acid (3,4-diCQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), and 4,5-dicaffoylquinic acid (4,5-diCQA) by MS, 1H NMR, and HPLC analyses were isolated from low-quality (immature) and commercial quality green coffee beans. The quantity of chlorogenic acid isomers (10.4 g/100 g), especially 5-CQA, in commercial green coffee beans [West Indische Bereiding (West India processing beans from Sumatra Island, Indonesia, WIB)] was higher than that in low-quality beans [9.1 g/100 g, Eerste Kwaliteit (Export low-quality beans from Java Island, Indonesia, EK-1, grade 4)], whereas little difference in diCQAs was detected between the two kinds of beans. The free radical scavenging activity of these isolates was evaluated in assay systems using DPPH free radicals and superoxide anion radicals generated by xanthine-XOD. The diCQAs showed strong (1.0-1.8-fold) free radical scavenging activity compared to commonly used antioxidants such as alpha-tocopherol and ascorbic acid. The potency order of superoxide anion radical scavenging activity was diCQAs > caffeic acid, CQAs > 5-FQA. The activities of the diCQAs were twice as effective as those of CQAs and 4 times as effective as that of 5-FQA. The diCQAs also exhibited more potent (2.0-2.2-fold) tyrosinase inhibitory activities compared to CQAs, arbutin, and ascorbic acid. The isolates exhibited antiproliferation activities in four cancer cell lines, U937, KB, MCF7, and WI38-VA. Among these, KB cells were most sensitive (IC50 = 0.10-0.56 mM).

Animals↗
PubMed1969

Possibilities for inter- and intracellular translocation of some icosahedral plant viruses.

Southern bean mosaic virus (SBMV) and Tomato ringspot virus (TomRV) were compared with regard to possible ways of inter- and intracellular translocation. The pore complexes in the nuclear membranes of nuclei in leaf palisade and mesophyll cells of several plant species commonly used in plant virus research were studied. The pore structure resembled that earlier described. The diameter of the pores was great enough to allow icosahedral plant viruses between 25 and 30 mmicro wide to move through. SBMV occurred in noncrystalline form in nuclei of infected cells. Although this virus forms paracrystalline structures when partially purified, no virus crystals were seen in the cytoplasm of cells containing high concentrations of SBMV. It was established that this virus could move through nuclear pores. TomRV was found in infected leaf tissue in low concentrations. This virus showed a tendency to crystallize even when present in low concentrations. TomRV was observed only in the cytoplasm, not in nuclei. This virus was present in plasmodesmata, indicating the possibility of cell to cell translocation of whole particles through these structures.

Cytopathogenic Effect, Viral↗
PubMed1991

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes.

Peroxisomes, glyoxysomes, glycosomes, and hydrogenosomes have each been classified as microbodies, i.e., subcellular organelles with an electron-dense matrix that is bound by a single membrane. We investigated whether these organelles might share a common evolutionary origin by asking if targeting signals used for translocation of proteins into these microbodies are related. A peroxisomal targeting signal (PTS) consisting of the COOH-terminal tripeptide serine-lysine-leucine-COOH has been identified in a number of peroxisomal proteins (Gould, S.J., G.-A. Keller, N. Hosken, J. Wilkinson, and S. Subramani. 1989. J. Cell Biol. 108:1657-1664). Antibodies raised to a peptide ending in this sequence (SKL-COOH) recognize a number of peroxisomal proteins. Immunocryoelectron microscopy experiments using this anti-SKL antibody revealed the presence of proteins containing the PTS within glyoxysomes of cells from Pichia pastoris, germinating castor bean seeds, and Neurospora crassa, as well as within the glycosomes of Trypanosoma brucei. Western blot analysis of purified organelle fractions revealed the presence of many proteins containing this PTS in both glyoxysomes and glycosomes. These results indicate that at least one of the signals, and therefore the mechanism, for protein translocation into peroxisomes, glyoxysomes, and glycosomes has been conserved, lending support to a common evolutionary origin for these microbodies. Hydrogenosomes, the fourth type of microbody, did not contain proteins that cross-reacted with the anti-PTS antibody, suggesting that this organelle is unrelated to microbodies.

Amino Acid Sequence↗
PubMed1994

The normal mycoflora of commodities from Thailand. 2. Beans, rice, small grains and other commodities.

Part of a comprehensive study of fungi occurring in commodities in Thailand, this paper reports results from 276 samples of mung beans, rice, sorghum and soybeans as well as other minor crops. Samples for major commodities were taken from farmers' stocks, middlemen and retail outlets, while those for minor crops were mostly from retail. Each sample was divided into two portions, one being examined in Bangkok and the second at North Ryde. Mycological examinations were carried out by direct plating after surface disinfection in chlorine. Media used were dichloran rose bengal chloramphenicol agar, dichloran 18% glycerol agar, Aspergillus flavus and parasiticus agar and dichloran chloramphenicol peptone agar. Fungi were identified to species level and percentage infection of samples calculated. The dominant fungus found in mung beans was Fusarium semitectum, which was present in 40% of samples and at a high level (18% of all seeds) overall. Aspergillus flavus was also found in 40% of samples, but only in 2% of seeds overall. Lasiodiplodia theobromae was the only other fungus exceeding 1% total infection. The major fungi found in soybeans were A. flavus (67% of samples; 6% overall) and Cladosporium cladosporioides (49% of samples; 9% overall). Storage fungi were more common in soybeans than the other commodities, indicating longer or adverse storage. Paddy rice contained high levels of a wide variety of field fungi, notably Fusarium semitectum, Bipolaris oryzae, and Curvularia, Phoma and Colletotrichum species. However, probably due to the heat generated by milling, milled rice contained very few fungi, which were mostly of storage origin. Only 3% of particles examined contained internal fungi. Infection in sorghum was typical of oilseeds, dominated by Aspergillus flavus, which was present in 86% of samples, with an overall infection rate of 12%. Beans other than soybeans were infected by a wide range of field fungi, but at low levels, with less than half of seeds examined being infected internally. With the exception of sorghum, the levels of mycotoxigenic fungi encountered were much lower than observed previously in nuts and oilseeds.

Edible Grain↗
PubMed2005

Diagnosis and management of G6PD deficiency.

Glucose-6-phosphate dehydrogenase deficiency, the most common enzyme deficiency worldwide, causes a spectrum of disease including neonatal hyperbilirubinemia, acute hemolysis, and chronic hemolysis. Persons with this condition also may be asymptomatic. This X-linked inherited disorder most commonly affects persons of African, Asian, Mediterranean, or Middle-Eastern descent. Approximately 400 million people are affected worldwide. Homozygotes and heterozygotes can be symptomatic, although the disease typically is more severe in persons who are homozygous for the deficiency. The conversion of nicotinamide adenine dinucleotide phosphate to its reduced form in erythrocytes is the basis of diagnostic testing for the deficiency. This usually is done by fluorescent spot test. Different gene mutations cause different levels of enzyme deficiency, with classes assigned to various degrees of deficiency and disease manifestation. Because acute hemolysis is caused by exposure to an oxidative stressor in the form of an infection, oxidative drug, or fava beans, treatment is geared toward avoidance of these and other stressors. Acute hemolysis is self-limited, but in rare instances it can be severe enough to warrant a blood transfusion. Neonatal hyperbilirubinemia may require treatment with phototherapy or exchange transfusion to prevent kernicterus. The variant that causes chronic hemolysis is uncommon because it is related to sporadic gene mutation rather than the more common inherited gene mutation.

Glucosephosphate Dehydrogenase Deficiency↗
PubMed1990

A characterization of epitopes on potato leafroll virus coat protein.

A panel of ten stable hybridoma cell lines secreting monoclonal antibodies (MAbs) specific for potato leafroll virus (PLRV) antigen, was produced in two fusion experiments with murine splenic and myeloma cells. Using different ELISA procedures and Western blotting it was shown that one MAb detected a continuous epitope and nine MAbs reacted with conformation-dependent ones. The conformation-dependent epitopes could be separated into two groups after alkaline treatment of the virions. The MAbs were further differentiated in competitive binding assays. Within the group of MAbs reacting with epitopes not sensitive to alkaline degradation, only two MAbs were directed to the same epitope. The MAbs detecting epitopes formed by the quaternary protein structure or by a protein subunit configuration sensitive to alkaline degradation, displayed positive cooperative binding among each other. In total, a minimum number of nine different, but overlapping, epitopes on the PLRV coat protein could be revealed. The immune response to PLRV antigen in rabbit appeared to be directed mainly towards epitopes recognized by three MAbs. Most MAbs displayed heterologous reactivity to other luteoviruses, i.e., tomato yellow top virus (TYTV), beet western yellow virus (BWYV), beet mild yellowing virus (BMYV), bean leafroll virus (BLRV), and different strains of barley yellow dwarf virus. Three MAbs solely reacted with PLRV and TYTV. Six MAbs gave different reaction patterns in these tests; one of these MAbs differentiated BMYV from BWYV, and another detected a common epitope on PLRV and BLRV, a serological relationship not reported previously to our knowledge.

Animals↗
PubMed2003

[Recommended diet for reflux spectrophotometry].

BACKGROUND: The spectrophotometric probe, which uses bilirubin as a marker for the detection of duodenoesophagic reflux is subject to interference from strongly colored foods, which can cause erroneously high bilirubin absorbance readings. To overcome this problem it is necessary to ingest a diet that is free from such substances. OBJECTIVE: To test the absorbance of 48 different food substances in an in vitro environment. MATERIAL AND METHOD: Dry foods were blended with water or milk and non-dry solid foods were blended undiluted. It was utilized the proper calibration recipient to test them. RESULTS: The absorbance of weakly colored foods was usually lesser than the commonly accepted threshold of 0.14, and the absorbance of strongly colored foods was usually above this. Thirty-two from the 48 substances tested are suitable when the absorbance threshold is set at 0.14, but scrambled eggs, lacteous flour mush, green beans, beetroot, carrot, chayote, squash, "baroa" potato, boiled corn, orange, cashew, purple grape, avocado, mango, papaya and peach can alter the results and must be avoided. CONCLUSION: From the foods evaluated, enough are suitable at the 0.14 threshold to enable a suitable diet to be constructed for most patients during Bilitec studies.

Absorption↗
PubMed2002

A systematic review of nonpharmacological and nonsurgical therapies for gastroesophageal reflux in infants.

BACKGROUND: Nonpharmacological and nonsurgical measures are often recommended for gastroesophageal reflux disease (GERD) in infants, despite ambiguous supporting evidence. OBJECTIVE: To conduct a systematic review of rigorously evaluated nonpharmacological and nonsurgical therapies for GERD in infants. DESIGN/METHODS: We searched online bibliographic databases, including MEDLINE, EMBASE, the Cochrane Collaboration and Clinical Trials Database, and alternative medicine databases for the terms gastroesophageal reflux and infants. We selected randomized controlled trials of nonpharmacological and nonsurgical GERD therapies in otherwise healthy infants. Data were extracted from the selected articles regarding reflux, emetic episodes and intraesophageal pH. RESULTS: We identified 43 relevant studies, of which 10 met the selection criteria. These studies examined positioning, pacifier use, and feeding changes. Positioning at a 60 degree elevation in an infant seat was found to increase reflux compared with the prone position. No significant difference was shown between the flat and head-elevated prone positions. The impact of pacifier use on reflux frequency was equivocal and dependent on infant position. The protein content of formula was not found to affect reflux. Although no study demonstrated a significant reflux-reducing benefit of thickened infant foods compared with placebo, 1 study detected a significant benefit of formula thickened with carob bean gum compared with rice flour (pH<4 for 5% vs 8% of time). Another study showed that if supplementing with dextrose 5% water or dextrose 10% water, the lower-osmolality fluid was associated with less reflux. CONCLUSIONS: Many conservative measures commonly used to treat GERD in infants have no proven efficacy. Although thickened formulas do not appear to reduce measurable reflux, they may reduce vomiting. Further studies with clinical outcomes are needed to answer questions about efficacy definitively.

Gastroesophageal Reflux↗
PubMed1997

Amino acid sequence of the basic subunit of 13S globulin of buckwheat.

A 26 kDa basic subunit of 13S globulin has been purified from grains of common buckwheat (Fagopyrum esculentum Moench). The amino acid composition of the protein closely matches the W.H.O. recommended values for a nutritionally balanced protein. The sequence of 17 N terminal amino acid residues of the protein revealed 73.3 and 66.7% homology with soya bean glycinin and pea legumin, respectively.

Amino Acid Sequence↗
PubMed1987

Risk factors for urinary tract infection.

The authors carried out a case-control study in 1982-1983 to investigate the possible influence of behavioral factors on the risk of urinary tract infection. Study participants were college women attending a student health service. Cases were 43 women with culture-confirmed urinary tract infection. There were two control groups: 149 women with upper respiratory infection and 227 women visiting the gynecology clinic. Using each set of controls, the study confirmed that sexual intercourse is a risk factor and that there is a dose-response effect for increasing levels of coital frequency. The study also found that use of the diaphragm was significantly associated with urinary tract infection (odds ratios 3.0, 2.3), an association which remained significant even after controlling for possible confounding by coital frequency. The findings did not show an association with many of the factors commonly believed to be important such as type of clothing worn and volume of fluids consumed.

Adolescent↗
PubMed1997

Assessment of cell surface glycoconjugates in normal, benign and malignant human nasal mucosa.

Aberrant glycosylation of proteins is a common characteristic of neoplastic changes. No reports exist relating cell surface glycoconjugates to normal, benign and malignant human nasal mucosa. Using lectin affinity histochemistry, glycoconjugate reactivities for peanut agglutinin (PNA), concanavalin A (Con A), Griffonia simplicifolia agglutinin II (GSA-II), soy bean agglutinin (SBA) and Ulex europaeus agglutinin l (UEA-I) were analysed in the following groups: normal, benign (polyp, papilloma, and inverted papilloma) and malignant (squamous cell carcinoma (SCC) alone, SCC arising in inverted papilloma, and adenocarcinoma). The positive rate of lectin staining was evaluated using a quantitative AutoCAD programme. We correlated glycoconjugate expression to clinical features, diagnosis, and malignant transformation. The positive rate of PNA after neuraminidase pre-treatment (NA-PNA) staining was higher in inverted papilloma, while all-negative in polyp and papilloma. NA-PNA staining may be used as a differential diagnostic tool. Both inverted papilloma portions and SCC portions of the SCC synchronized with inverted papilloma subjects showed similar Con A and NA-PNA staining patterns. The biological characteristics define inverted papilloma as a pre-malignant neoplasm. The positive rate of PNA staining was significantly higher in inverted papilloma (inverted papilloma transformed to SCC) compared to inverted papilloma alone. Hence, PNA staining may predict malignant transformation of inverted papilloma. However, further investigations are required to prove this possibly worthwhile prognostic marker.

Adenocarcinoma↗
PubMed1983

Sodium values of Chinese condiments and their use in sodium-restricted diets.

Counseling a Chinese patient on a low-sodium diet can be an arduous task for the dietitian. Special consideration of cultural beliefs, food preferences, and food practices must be incorporated into the individualized instruction. Equally important is the need for familiarity with the sodium values of frequently consumed foods and condiments indigenous to the Chinese population. To date, published sodium determinations for such items are quite limited. Representative samples of commonly used Chinese seasonings and sauces were collected. Analysis was performed by Atomic Absorption Spectroscopy. Many of the condiments often forbidden in the sodium-restricted diet contained less than 200 mg. sodium per teaspoon in all the samples tested. These included: sweet bean sauce, rinsed and unrinsed fermented black beans, hoisin sauce, satay sauce, fermented bean cake, and rinsed and unrinsed dried shrimp. These data can give the dietitian the option of calculating these often "forbidden" items into the sodium-restricted diet. Factors to be considered are the patient's typical meal pattern, situational needs, comprehension level, degree of compliance, and other available information.

California↗