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Dental follicle cell-conditioned medium enhances the formation of osteoclast-like multinucleated cells.

An influx of mononuclear cells and the subsequent increase of osteoclasts around tooth germs suggests that the dental follicle (DF) regulates or influences bone resorption required for tooth eruption. In order to study the effects of DF cell products on osteoclast formation during tooth eruption, a conditioned medium (CM) was created in which DF cells were added to mouse bone marrow cultures. Tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated cells were formed in the presence of 10 nM 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. The CM, dose-dependently, stimulated the formation of TRAP-positive cells in the presence of 1,25(OH)2D3 for 14 days culture. The number of these cells decreased due to degradation in the control culture. A semi-solid methylcellulose assay in the presence of CM showed little expression of colony-stimulating activity. These results suggest that the DF cells of a developing tooth produce factor(s) that enhance osteoclast formation and bone resorption necessary for tooth eruption.

Acid Phosphatase↗

Immediate placement and provisionalization of maxillary anterior single implants: a surgical and prosthodontic rationale.

Maintaining the existing gingival architecture of a failing maxillary anterior tooth is essential in achieving an optimal aesthetic result. Immediate implant placement has been advocated to minimize tissue loss following extraction. The incorporation of a customized temporary abutment and immediate fixed provisionalization facilitates maintenance of the gingival architecture for optimal aesthetics and eliminates the need for a removable provisional prosthesis during the healing phase. This article describes the surgical and prosthodontic approach of this procedure as well as its clinical rationale.

Acrylic Resins↗

A radiographic study of rostral tooth development in young Friesian cattle.

Twenty-nine British Friesian cattle fed the same diet, slaughtered at various weights and including bulls, steers (castrated males) and females (heifers) were examined. The stage of their rostral tooth development was recorded both visually and radiographically. Insufficient animals were present in each sex and slaughter weight treatment group to allow any comparison between them. When the data for each sex were compared, no statistically significant differences were seen between the mean ages at which bulls, steers and heifers attained each stage of dental development as determined from their radiographs. The results for all 29 cattle were then combined. This combined data showed a relationship between age and those stages of tooth formation which could be differentiated by examination of the X-ray films. As most phases of tooth formation occurred within well defined limits, it is suggested that radiographs of rostral tooth development might be used to estimate the age of cattle.

Age Factors↗

[The effect of radionuclides on the rat masticatory organ].

The effects of incorporated radium nuclides were examined clinically, roentgenologically, histologically, gamma-spectrometrically, and autoradiographically in animal experiments. The experimentally induced resorption can be compared with alterations observed with man.

Animals↗

Evaluation of the topical effect of alendronate on the root surface of extracted and replanted teeth. Microscopic analysis on rats' teeth.

The treatment of choice for tooth avulsion is replantation. The ideal replantation should be realized as quickly as possible, or at least, the avulsed tooth should be kept in an adequate solution to preserve the periodontal ligament attached to the root. If that is not possible, treatment of the radicular surface should be done in order to prevent radicular resorption. The purpose of this study was to test sodium alendronate as a substance for topical treatment of the radicular surface of avulsed teeth in an attempt to prevent the occurrence of dental resorptions. Fifty-four rat maxillary right central incisors were extracted and replanted. Group I--extra-alveolar dry period of 15 min, intracanal dressing with calcium hydroxide (CALEN, S.S. White, Artigos Dentários LTDA, Rio de Janeiro, Brazil) and replantation; Groups II and III - extra-alveolar dry periods of 30 and 60 min, respectively, immersion in 1% sodium hypochlorite for 30 min for removal of the periodontal ligament, washing in saline solution for 5 min, and treatment of the radicular surface with 3.2 mg/l sodium alendronate solution for 10 min. Intracanal dressing with calcium hydroxide and replantation followed. At 15, 60, and 90 days post-reimplantation, the animals were killed and the samples obtained and processed for microscopic analysis. The results indicated that sodium alendronate was able to reduce the incidence of radicular resorption, but not of dental ankylosis. No significant differences were observed regarding variations in the extra-alveolar periods among the groups.

Alendronate↗

Evidence for a resorption inhibitor in dentin.

The ability of stimulated and unstimulated peritoneal macrophages to spread in vitro on different inorganic and organic components of dental tissues was studied in order to establish morphologic evidence in favor of a resorption inhibitor in dentin. Macrophages were seeded onto enamel, dentin, predentin, demineralized dentin and collagen-coated coverslips and examined with scanning electron microscopy after varying incubation periods. The cells readily attached and spread on enamel, dentin and collagen-coated coverslips. Cells attached but showed no signs of spreading when incubated on predentin or demineralized dentin. It was concluded that the resistance to resorption of predentin and dentin rests in the organic, non-collagenous component of the tissue, indicating the presence of a resorption inhibitor in dentin.

Animals↗

Autotransplantation of teeth in cases with agenesis or traumatic loss of maxillary incisors.

The purpose of this study was to investigate the prognosis of 50 consecutively autotransplanted teeth to the maxillary incisor region. Thirty-five were transplanted in cases of traumatic injuries, eleven in cases of agenesis and four in cases with impaction of maxillary canines. The average follow-up time was 7 years and 6 months (range 4-17 years). The transplants were examined clinically in respect of tooth vitality and radiographically in respect of root canal obliteration, periradicular changes, root formation, and root length. The results show a success rate of 82 per cent. It is concluded that the procedure of tooth transplantation has the advantage of maintaining or restoring alveolar bone volume and gives the opportunity to replace a missing tooth without involvement of neighbouring teeth as prosthetic abutments.

Adolescent↗

A flow cytometric analysis of the biodefensive response of deciduous tooth pulp to carious stimuli during physiological root resorption.

The present study observed biodefensive responses of deciduous tooth pulp to advancing caries lesions and analysed the role of physiological root resorption on pulpal defense potential. For this purpose, immunocompetent cell content of deciduous tooth pulp was examined using flow cytometry. A total of 49 deciduous incisor and molar teeth at various stages of physiological root resorption (carious or non-carious) to be extracted for clinical reasons were used in this study. Teeth were classified according to carious lesion depth and root resorption stage. CD3+ lymphocytes were observed to be most prevalent in the pulp and to show remarkable increase along with increase in carious lesion depth. Numbers of CD8+ lymphocytes also increased significantly as carious lesions approached the pulp. However, increase in the number of CD3+ and CD8+ cells did not significantly alter CD4+/CD8+ ratios. The study also found that while B-lymphocytes increased significantly in association with root resorption, there were no significant differences in B/CD3+ lymphocyte ratios. Thus, there was no evidence of irreversible pulpal pathosis in any groups. It can be concluded that pulp maintains its healing and defense capacity against advancing carious lesion and progressive root resorption in deciduous teeth.

Adolescent↗

Treatment of replacement resorption by intentional replantation, resection of the ankylosed sites, and Emdogain--results of a 6-year survey.

The present clinical study investigated the outcome of intentional replantation using resection of the ankylosed sites of the root, extraoral endodontic treatment using titanium posts and Emdogain for periodontal healing following trauma-related ankylosis. During an evaluation period of 6 years, 16 ankylosed teeth affected by replacement resorption were treated as described. Evaluation parameters before treatment and during the follow-up period included Periotest scores, percussion sound and periapical radiographs. All findings were compared to those of the adjacent teeth. In a second accident, one tooth was lost after 7 months and was excluded as a dropout. Ankylosis did not recur in seven replanted teeth, which were observed for an average of 52.3 months (range: 24-68 months). Ankylosis recurred in eight teeth after an average period of 12 months (range: 4-26 months). An infraocclusion, normal or only slightly reduced Periotest scores and normal percussion sound were preoperatively found in six of seven successfully replanted teeth, which corresponded to a relatively small area of ankylosis. The majority of the teeth showing recurrent ankylosis preoperatively presented with normal position, negative Periotest scores and a high percussion sound which corresponded to an extended area of ankylosis. Statistically significant relationship between preoperative findings and the treatment outcome (P = 0.031) have become apparent. The results indicate that the treatment of minor areas of ankylosis by intentional replantation, resection of the ankylosed sites and Emdogain appeared to prevent or delay the recurrence of ankylosis in 7 of 15 teeth.

Adolescent↗

Prevalence of dental resorptive lesions in Swedish cats.

Ninety-six, randomly selected Swedish cats were evaluated for the presence of dental resorptive lesions. All cats were examined while receiving general anesthesia. Diagnosis was based on oral examination and full-mouth, intraoral dental radiographs. Information concerning age, sex, vaccination status, eating habits, food type, environment (indoor or outdoor housing), oral, discomfort, dental care, and medical treatment was recorded. Hematologic samples included analysis for FeLV, FIV, and calcivirus. Of the cats examined in this study, 32% had gross or radiographic signs of dental resorptive lesions. There was a positive relationship between the occurrence of dental resorptive lesions and increasing age.

Animals↗

Observations of the periodontal ligament and cementum in cats with dental resorptive lesions.

The etiology of feline dental resorptive lesions is unknown, but some evidence suggests that interactions between components of the periodontium may be initiating factors in the development of these lesions. In the present study, 22 clinically normal teeth were harvested from 7 cats. The teeth and periodontium were radiographed and examined histologically. In addition, 14 of the 22 teeth were examined histometrically. Two teeth were histologically normal with an open apical foramen and two were normal with a closed apical foramen. Histological evidence of periodontal ligament degeneration without cementum resorption was observed in 8 teeth, and varying degrees of cementum resorption were observed in 10 teeth. Mandibular molar and premolar teeth had distal drift, and mandibular canine teeth had mesial drift. Alterations in the periodontal ligament may represent a preclinical stage of dental resorption.

Animals↗

Repeated human tooth allograft after a previous rejection.

A case has been presented in which a humantooth allograft was performed. It failed in a reasonably expected manner. Another tooth allograft from the same donor to the same host was then performed. It is currently functioning two years postoperatively. This seems to defy the law of second-set reaction. Various explanations for the response to this repeated tooth allograft have been discussed. It is concluded that possibility of repeating tooth allografts, even after a first allograft has failed, should be considered.

Anodontia↗