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At least 775 records · Page 43Linked to original sources
PubMed2002

The importance of the seated condylar position in orthodontic correction.

It has been proposed that the discrepancy between the seated and unseated condylar position be identified and eliminated when the occlusion is reorganized. Identification of this discrepancy is most accurately accomplished through the use of diagnostic casts that have been taken from a deprogrammed patient and mounted in the seated condylar position on a semiadjustable articulator through an estimated facebow transfer. The amount and direction of any discrepancy is determined three dimensionally with condylar position instrumentation.

Adult↗
PubMed2002

Ultimate anchorage control.

Titanium microscrews represent an exciting new modality for orthodontic treatment. Retraction, protraction, intrusion and extrusion of anterior and posterior teeth can occur without harmful side effects on adjacent teeth and without reliance on patients' cooperation in wearing elastics, facemasks, or headgears. There are no moving parts in the mechanism, and movements in one jaw occur independently or the other. The results include greater patient acceptance, more comfort, and less breakage of appliances.

Adolescent↗
PubMed2002

Clinical evolution of the Invisalign appliance.

The Invisalign System of tooth movement has been available to orthodontists since 1999 and has now become available to the entire dental profession. This paper explores the role of this system within the dental armamentarium and describes the clinical evolution of the appliance, based on a feasibility study initiated at the University of the Pacific in 1997.

Adolescent↗
PubMed2002

Stabilization of mRNA expression in whole blood samples.

BACKGROUND: Accurate quantification of mRNA in whole blood is made difficult by the simultaneous degradation of gene transcripts and unintended gene induction caused by sample handling or uncontrolled activation of coagulation. This study was designed to compare a new blood collection tube (PAXgene Blood RNA System) and a companion sample preparation reagent set with a traditional sample collection and preparation method for the purpose of gene expression analysis. METHODS: We collected parallel blood samples from healthy donors into the new sample collection tubes and control EDTA tubes and performed serial RNA extractions on samples stored for 5 days at room temperature and for up to 90 days at 4 and 20 degrees C. Samples were analyzed by Northern blot analysis or reverse transcription-PCR (RT-PCR). RESULTS: Specific mRNA concentrations in blood stored in EDTA tubes at any temperature changed substantially, as determined by high-precision RT-PCR. These changes were eliminated or markedly reduced when whole blood was stored in PAXgene tubes. Loss of specific mRNAs, as measured by RT-PCR, reflected total RNA depletion as well as specific mRNA destruction demonstrated by Northern blot analysis. The salutary effects of PAXgene on mRNA stabilization extended to blood samples from eight unrelated donors. CONCLUSIONS: Compared with whole blood collected in EDTA tubes and extracted by an organic method, the PAXgene Blood RNA System reduced RNA degradation and inhibited or eliminated gene induction in phlebotomy whole blood samples. Storage of whole blood samples in PAXgene tubes can be recommended for clinically related blood samples that will be analyzed for total or specific RNA content.

Blood Specimen Collection↗
PubMed2002

[Everyday management in mixed dentition and Tweed's concept].

The topic of this paper is to emphasize the strategy of occlusion management during childhood. The orthodontist intervenes during the mixed dentition period, for transversal and antero-posterior cross-bites, for vertical problems such as open-bites and deep-bites, but he also deals with habits and functional diseases. Treatment has to be simple and short. Most of the appliances are fixed. The author presents some clinical cases, and then defines "short-cuts" as steps of therapy that shorten the active treatment. To conclude, a case report emphasizes the fact that before any diagnosis based on the lower incisor, the clinician must ensure that the incisor location is closely related to a malocclusion and not to an unbalanced neuromuscular function.

Adolescent↗
PubMed2002

[Indications for orthognathic surgery, the limitations of orthodontics and of surgery].

The different sagittal, vertical or transversal skeletal dysmorphosis can be treated with orthodontic or orthopaedic appliances. Certain limits of treatment will be due to the age of the patient, the importance of the skeletal dysmorphosis, technical limits of the orthodontic appliances or the motivation and compliance needed from the patient. For this reason an orthodontic treatment combined with orthognathic surgery may be indicated. Nevertheless the combined orthodontic-surgical treatment has also its limitations and specific treatment needs from orthodontist and maxillo-facial surgeon.

Adolescent↗
PubMed2002

[Pre- and post-surgical orthodontic objectives and orthodontic preparation].

Orthodontics in combination with orthognathic surgery is a possibility today for correcting sagittal, vertical and transversal dysmorphosis. To do this, orthodontic preparation before surgery is necessary. In the sagittal plane, dental compensations should be removed. In the vertical plane, levelling of a severe mandibular SPEE curve should be accomplished postsurgically, and transverse coordination obtained. Orthodontic preparation is very specific to the type of dysmorphosis: class II malocclusion, class III malocclusion or in open bite. Postsurgical orthodontic treatment is used to finalize tooth alignment as in conventional orthodontics.

Craniofacial Abnormalities↗
PubMed1992

[Implantation model in vitro].

Implantation is thought to be interactive and synchronized process between mother and embryo, however, the mechanisms of the early implantation process remain unelucidated. Therefore, the effectiveness of in vitro fertilization and embryo transfer is still poor. As a result; immunohistochemically, type IV collagen and other extracellular matrix components were detected mainly below the epithelial layer. Type I and III collagens were detected diffusely in the stromal layer. In the lower stromal layer and superficial myometrial layer, type V collagen was detected. Human endometrial epithelial cells performed the re-epithelialization following glandular formation. Rabbit endometrial cells performed also re-epithelialization following the fold formation. The stromal cells were invaded into the inner layer of the folding. Estrogen added to the culture media stimulated the glandular formation. Progesterone administration after estrogen priming did not affect the glandular formation, however, the proliferation of the superficial epithelium and the re-epithelialized area were increased. Rabbit blastocysts successfully attached and implanted into the reconstructed endometrium. The development of the implanted embryos was morphologically normal. Human cultured trophoblast cells attached, invaded and penetrated into the extracellular matrix components. On the other hand, using type V collagen coated dishes, trophoblast cells could invade the stromal layer, however, type V collagen layer did not permit the trophoblast cells to invade into the collagen layer. In vivo, type V collagen, expressed in the lower stromal layer and the surface of the myometrium, may play a role to maintain the early embryo in the decidual compartment. EGTA inhibited the attachment of the blastocysts. Anti-laminin antibody and RGDS peptide, attachment domain of laminin, also inhibited the implantation. These findings suggested that the Ca2+ dependent process was necessary for the attachment between the trophoblasts and the endometrial cells, and then the implantation process was triggered after the attachment to the laminin in basement membrane. The endometrial tissue, obtained from the infertile patient, was cultured on the basement membrane extract with serially obtained maternal serum. Addition of the maternal serum after proper administration of pFSH, high estrogen conditions were made in the culture dishes. These conditions increased the height of the glandular structure and relatively decreased the area of the surface epithelium. Decreased the area of surface epithelium affected the rate of the attachment. Endometrial cell culture system associated with functional and morphological characteristics was established. Serial observation of the endometrial cells in this system revealed the rabbit and the human implantation process, and the embryo-endometrial interaction.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗