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At least 775 records · Page 43Linked to original sources
PubMed1995

[First- time isolation of the peste des petits ruminants (PPR) virus in Chad and experimental induction of the disease].

In Chad, in 1993-1994, investigation into "peste des petits ruminants" (PPR) outbreaks were carried out in flocks of Sahelian goats. Although in the early 1993 outbreaks the virus had not been isolated yet, a serological prevalence (34%) was observed using ELISA test in 475 sera collected in the infested area. The virus was then isolated for the first time in Chad and typical PPR cases were observed in Sahelian goats, known to be little sensitive to the virus. To experimentally induce the disease, goats were inoculated with suspensions of lymph nodes or lungs, collected from sick animals. Lung suspensions induced the most serious symptoms. Suspensions of lungs and mesenteric lymph nodes were positive by agar-gel immunodiffusion test against PPR anti-serum, while prescapular lymph nodes remained negative. Characterization of the isolated virus, in particular with regard to its genomic identity, should be investigated.

Animals↗
PubMed2001

Idiotypic-anti-idiotypic B cell interactions generated against a protective antigen of a morbillivirus in mice.

The idiotypic network theory (N. K. Jerne, Ann. Immunol. 125, 373-389, 1974) predicts that any antibody that can be made by an individual would have its preexisting specific complementary B cells in its germline repertoire. We transplanted syngeneic BALB/c mice with live hybridoma cells and demonstrated the simultaneous presence of interacting idiotypic and anti-idiotypic B cells in an individual animal by immuno-cytoadherence assays. Furthermore, we demonstrate that interacting B cells displaying idiotypic and anti-idiotypic antibodies are subjected to lysis by complement. It is therefore tempting to speculate that this complement-sensitive interaction between idiotypic and complementary anti-idiotypic B cells in vivo may provide a mechanism for the regulation of B cell populations.

Animals↗
PubMed1994

Detection of novel RNA viruses: morbilliviruses as a model system.

Recent advances in molecular detection of viral-specific nucleic acid sequences have facilitated the search for pathogenic viruses in clinical samples. Primers for the polymerase chain reaction can be targeted to unique or conserved regions of the viral genome. While the design of primers must be based on known sequences, it has been demonstrated in bacteria that amplification with primers which hybridize to highly-conserved regions of the genome can be exploited to detect new agents. By taking advantage of sequence conservation among diverse members of a viral genus, we designed PCR primers capable of hybridizing to three different members of the genus Morbillivirus for which sequence information is available. Simultaneous amplification with primers for the porphobilinogen deaminase gene, a constitutively expressed gene in all cells, confirmed the presence of amplifiable RNA. Sequence alignments for the matrix and nucleoprotein genes suggested a highly conserved region of the nucleoprotein gene as a likely target for gene amplification. Morbillivirus primer pairs were chosen from the nucleoprotein gene, which successfully amplified measles virus, canine distemper virus, and phocine distemper virus. In a single reaction which combines cDNA synthesis by reverse transcription of the RNA genome with the polymerase chain reaction (RT PCR reaction), amplification with these primers detected as little as 0.1 pfu (plaque forming unit) of measles virus and 1 TCID50 of canine distemper virus. These Morbillivirus primers can be used to search for novel members of the genus which are likely to share sequence similarity in these highly conserved regions.(ABSTRACT TRUNCATED AT 250 WORDS)

Base Sequence↗
PubMed1985

Hyperimmune serum in the control of peste des petits ruminants.

The value of administration of hyperimmune serum in the control of peste des petits ruminants was investigated in goats at different stages of the disease. A group of the goats was given hyperimmune serum intravenously at the fever stage of temperature of 40.5 degrees C or above; another group showing no elevation of temperature but with other clinical signs of the disease were also given hyperimmune serum. Results indicated that hyperimmune serum was very effective in reversing the process of the disease if administered at the fever stage but not in animals that had progressed past the fever stage. The goats given the hyperimmune serum survived for 10 days before showing evidence of reinfection.

Animals↗
PubMed1982

The use of an enzyme-labelled assay as an aid to reading micro virus-neutralisation tests.

An indirect immunoperoxidase technique has been developed for the detection of virus-infected cells after completion of a micro virus-neutralisation test. This allows visual rather than microscopic reading of the plates, which is less time-consuming and does not require a technician skilled in distinguishing between specific viral cytopathic effect and non-specific cytotoxicity. The technique proved to be more sensitive than microscopic reading for the detection of low levels of antibody and could be of great use for other viruses which do not produce a marked cytopathic effect on cells or have a slow growth rate, making development of a standard virus-neutralisation test very difficult.

Antibodies, Viral↗