The medico-legal precipitin test for human blood.
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Schistosomiasis has special characteristics in Venezuela, since the majority of the infected population is asymptomatic and the elimination of eggs is very low, difficulting in this way parasitologic diagnosis. It is thus necessary to develop sensitive and specific immunologic methods to be applied in field surveys. The present study compares two serologic tests for Schistosomiasis, the enzyme linked immunosorbent assay (ELISA) with eggs (SEA) and adult (AWA) antigens and the traditional circumoval precipitation test (COPT). Sensitivity and specificity of the test were performed being 100% and 92% in COPT, 79% and 71% in ELISA-SEA and 71% and 91% in ELISA-AWA respectively. Advantages and disadvantages of each test are discussed.
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The agar-gel precipitation test (AGPT) was compared with Enzyme Immunoassay (EIA) for the detection of Chlamydia trachomatis, 164 women being screened for chlamydial antibodies and antigens in their sera and in endocervical specimens, respectively. The AGPT showed good correlation with EIA in the 164 paired sera and endocervical specimens, resulting in 27 (16.5%) and 22 (13.4%) positive results, respectively (P greater than 0.05). The overall sensitivity of the AGPT compared to EIA was 86.4% (19 of 22), and the specificity was 94.4% (134 of 142).
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