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Use of a low ionization potential dopant in a kinestatic charge detector: experimental findings.

The broadening of the line spread function (LSF) in the drift direction with increasing drift distance in the kinestatic charge detector is substantially reduced when small amounts (less than 1%) of trimethylamine [(CH3)3 N] are added to the x-ray detection medium (krypton or xenon). The LSF of a mixture of Kr and 0.01% trimethylamine (TMA) was measured as a function of distance at 15, 25, and 35 atm absolute pressure. The full width at half-maximum (FWHM) of the LSF was reduced from about 1.0 mm to less than 0.5 mm at a drift distance of 4.0 mm for the three pressures. The LSF's of mixtures of xenon and TMA at concentrations ranging from 0.0004% to 0.4% in one run and 0.06% to 4.0% in a second run were measured at a constant pressure of 20 atm. The FWHM of the LSF was reduced from 0.6 to 0.4 mm at 4.0 mm for the xenon measurements. The optimum concentration of TMA in Xe was found to be in the neighborhood of 0.1%. The use of TMA reduced the drift distance-dependent LSF broadening to the level expected from ionic diffusion, space charge repulsion, and electric field nonuniformity, and it may be possible to reduce the 0.4-mm FWHM plateau through the use of an improved Frisch grid design. Observation of negative charge carriers showed that electron attachment increases with increasing TMA concentration, although this could be caused by impurities in the TMA. The implications of these results are discussed in terms of extending the maximum drift distance attainable in a kinestatic charge detector.

Humans↗

Atmospheric particulates: specific surface areas and densities.

Suspended particulates in Pittsburgh air were collected on glass-fiber filters. The specific surface areas of particulates brushed from the filter surface varied from 1.55 to 4.51 square meters per gram when measured by the Brunauer-Emmett-Teller (BET) method with nitrogen and krypton, after 8-hour degassing of the samples at 25 degrees C. Specific surfaces of the same samples varied from 4.3 to 8.00 square meters per gram after 4-hour degassing at 200 degrees C. Bulk densities and densities of samples were 0.49 to 0.64 and 2.0 to 2.6 grams per cubic centimeter, respectively. These data provide some basis for explanation of unpredictable responses reported after inhalation of mixtures of pollutant gases and particles by animals and man; they should also assist in interpretation of gas-solid phase reactions in the atmosphere.

Aerosols↗

Growth responses of Neurospora crassa to increased partial pressures of the noble gases and nitrogen.

Buchheit, R. G. (Union Carbide Corp., Tonawanda, N.Y.), H. R. Schreiner, and G. F. Doebbler. Growth responses of Neurospora crassa to increased partial pressures of the noble gases and nitrogen. J. Bacteriol. 91:622-627. 1966.-Growth rate of the fungus Neurospora crassa depends in part on the nature of metabolically "inert gas" present in its environment. At high partial pressures, the noble gas elements (helium, neon, argon, krypton, and xenon) inhibit growth in the order: Xe > Kr> Ar >> Ne >> He. Nitrogen (N(2)) closely resembles He in inhibitory effectiveness. Partial pressures required for 50% inhibition of growth were: Xe (0.8 atm), Kr (1.6 atm), Ar (3.8 atm), Ne (35 atm), and He ( approximately 300 atm). With respect to inhibition of growth, the noble gases and N(2) differ qualitatively and quantitatively from the order of effectiveness found with other biological effects, i.e., narcosis, inhibition of insect development, depression of O(2)-dependent radiation sensitivity, and effects on tissue-slice glycolysis and respiration. Partial pressures giving 50% inhibition of N. crassa growth parallel various physical properties (i.e., solubilities, solubility ratios, etc.) of the noble gases. Linear correlation of 50% inhibition pressures to the polarizability and of the logarithm of pressure to the first and second ionization potentials suggests the involvement of weak intermolecular interactions or charge-transfer in the biological activity of the noble gases.

Argon↗

Investigating the small lung: which imaging procedure?

Ventilation and perfusion radionuclide lung scans, using krypton 81m and technetium-99m macroaggregates, were performed together with a variety of other imaging procedures in 18 children aged between 1 week and 13 years in whom radiology had shown a small lung. Radionuclide scans provided an assessment of regional ventilation and perfusion unobtainable by other means, and 4 main categories of disturbed function could be seen in the radiological small lung--namely, absence of ventilation and perfusion, absent perfusion with preserved ventilation, generalised or segmental decreases in ventilation, and perfusion and segmental perfusion defects in areas of decreased ventilation. The clinical history and other imaging procedures, including fluoroscopy and penetrated mediastinal views, enabled a firm diagnosis to be made in each of these 18 patients. In 3 main pulmonary arteries were absent, 2 had lung aplasia, 2 had lobar aplasia, 9 had varying degrees of pulmonary hypoplasia (two with additional sequestrated segments), 1 had lobar emphysema, and 1 had post-infective lung mal-development (MacLeod's syndrome).

Child↗

Ventilation and perfusion scans in the preoperative assessment of bronchial carcinoma.

Ventilation (krypton -81m) and perfusion (technetium -99m) lung scans were obtained in a consecutive series of 21 patients shortly before thoracotomy for proven or suspected carcinoma of the bronchus. In most patients ventilation and perfusion were impaired equally and the scan abnormality corresponded to the bronchoscopic and radiological findings. Unexpectedly large defects in the perfusion scan were seen in three patients, all of whom had extensive neoplastic involvement of the mediastinum at thoracotomy, but 11 other patients had mediastinal involvement which was not suspected from the scan. Ventilation scanning was useful in the prediction of postoperative ventilatory capacity in two patients who underwent pneumonectomy. We conclude that ventilation and perfusion scans are not sensitive indicators of neoplastic involvement of the mediastinum but they are valuable for the prediction of postoperative lung function.

Adult↗

Streamlining of air flow in the upper airways and trachea.

Streamlining of air flow in the upper airways and trachea has been investigated by inhaling 81m krypton through each side of the nose separately and counting over the lung fields with a gamma camera. 81m Kr inhaled through one nostril was uniformly mixed in the air stream by the time the carina was reached. Gas mixing occurs probably by a combination of diffusion and turbulent flow.

Biophysical Phenomena↗

Role of lung scanning in assessing the resectability of bronchial carcinoma.

Ventilation and perfusion lung scans were performed in 58 patients before operation for bronchial carcinoma to determine in which patients the lung scan was most useful for assessing mediastinal spread and resectability of the tumour. Perfusion of the affected lung was less with larger and more centrally situated tumours. Perfusion was also less for left-sided than for right-sided tumours but this is explained by the normal differential perfusion of the right and left lungs. The lung scan was unhelpful in predicting resectability of peripheral tumours, but with central tumours if perfusion of the affected lung was less than 25% of the total perfusion the lesion was likely to be non-resectable because of spread to the mediastinum. Airways obstruction was present in 67% of the patients but did not interfere with the interpretation of the scans. In most cases ventilation scans provided no additional information, and the use of krypton-81 m as a sensitive indicator of regional ventilation did not improve on the predictive value of the perfusion scan.

Carcinoma, Bronchogenic↗

Effects of posture on the distribution of pulmonary ventilation and perfusion in children and adults.

In the adult the distributions of ventilation and of perfusion show the same directional dependence on gravity. In children, however, the distribution of ventilation in response to gravity is the reverse of that seen in adults. The aim of the current study was to determine whether perfusion showed the same reversal in children or followed the adult pattern. Distribution of perfusion was measured with intravenous technetium-99m macroaggregated albumin and distribution of ventilation with inhaled krypton-81m. Eighteen children and seven adults were studied; they had been referred for lung scanning for various respiratory problems. The effect of gravity was examined by giving aliquots of macroaggregated albumin and 81mKr by inhalation to the subject in the supine and the lateral decubitus position. Counts in the dependent lung were compared with those in the upper lung. The dependent lung in the lateral decubitus position received more of the total perfusion than it did in the supine position in seven children with a normal chest radiograph (mean 7.0%, range 4.8-10.9% more) and in 11 children with an abnormal radiograph (mean 3.4%(0.1-10.0%)). Ventilation, however, changed in the opposite direction, falling by 7.1% (-3.2% to -12.8%) in five children with a normal chest radiograph and 11.2% (-2.8% to -19.3%) in eight children with an abnormal radiograph. Fractional V/fractional Q (an index of the ventilation:perfusion ratio) decreased in the dependent lung in the children when they moved from the supine to the decubitus position. The same directional change was recorded in adults, but it was significantly less than in the children, irrespective of whether the chest radiograph was abnormal. In children and adults with various respiratory problems the effect of posture on the distribution of perfusion is similar.

Adult↗

Three-dimensional regional cerebral blood perfusion images with single-photon emission computed tomography.

Twenty-seven patients were studied for cerebral blood perfusion abnormalities using krypton 81m. A solution of 81mKr was continuously infused into an internal carotid artery, and single-photon emission computed tomograms were recorded. The transverse section images using 81mKr represented the three-dimensional regional cerebral blood perfusion, and this technique seemed to be useful for evaluating the regional cerebral blood perfusion in the deep brain.

Cerebrovascular Circulation↗

Transport of inert gases in mammalian myocardium: comparison with a convection-diffusion model.

Because tracer techniques are gaining an increasing importance for imaging flow (and metabolism) in the heart, experimental evidence is needed on the role of convection and diffusion in the transcoronary transport of solutes. In the present work, the transport of four different inert gases through the coronary system is studied in five closed-chest dog experiments and is compared with a digital multicapillary convection-diffusion model. Transport may be defined as flow dependent, as judged by the gross similarity of shape of the time-normalized dilution curves. However, the results show that the transcoronary transport of helium and xenon is more dispersed than that of argon and krypton, probably because of differences in diffusibility and solubility. A comparison of the animal and model experiments emphasizes the importance of diffusive transport of the gases. It is suggested that there is a diffusion shunt that is mainly located within the capillary network itself rather than between conduit vessels. Only for helium (which has the highest diffusivity) was a small arteriovenous shunt fraction seen that is thought to bypass the capillary exchange region. The conclusion is that although there is evidence of diffusional shunting at a capillary level, the inert gas kinetics in the heart are compatible with a basically flow-limited transport.

Animals↗

Neonatal changes in renal blood flow distribution in puppies.

The intrarenal distribution of blood flow was studied in 31 newborn mongrel puppies from 18 h to 70 days using xenon washout and krypton autoradiography. Mean renal blood flow increased from 0.39 plus or minus 0.05 ml/g per min (SE) the 1st wk to 2.06 plus or minus 0.12 ml/g per min at 6 wk. During the 1st wk of life renal cortex was perfused homo-geneously at 0.88 plus or minus 0.19 ml/g per min (SE) and accounted for 35 plus or minus 4% of the renal blood flow. During the 2nd wk a narrow, rapidly perfused zone of outer cortex was identified which was perfused at 3.35 plus or minus 0.26 ml/g per min, received 19.53 plus or minus 5.05% of the total renal blood flow, and represented 15 plus or minus 4% of the mass of the total cortex. The inner cortex and outer medulla at this time received 53.40 plus or minus 4.12% of the flow at 1.07 plus or minus 0.08 ml/g per min. Outer cortical flow increased with age reaching adult values by about 6-10 wk when the rapidly perfused area represented 40 plus or minus 8% of the cortex. These changes are parallel to the results of previously reported studies with microspheres in newborn puppies and are compatible with the well established maturational changes noted in neonates of several species. They represent the first gas-washout studies in animals during the first 6 wk of life.

Age Factors↗

Perfusion and ventilation isotope lung scans in constrictive bronchiolitis obliterans. A series of three cases.

Constrictive bronchiolitis obliterans (BO) has been defined as a syndrome of airflow limitation due to bronchiolar and peribronchiolar inflammation and fibrosis. However, chest roentgenograms are frequently normal, and alternative radiographic evaluation or other manifestations of the disease would be required. Here we report three patients with BO. We performed a technetium-99(m)-labeled macroaggregate human albumin lung perfusion scan and a ventilation scan using krypton-81(m). We observed multiple matched defects in the perfusion and ventilation scans in these patients, which represent narrowing or obliteration of the bronchiolar lumen and its adjacent pulmonary arteriole. We propose that this is a useful diagnostic approach for an imaging study of BO.

Adult↗

The effects of nifedipine on acute experimental myocardial ischemia and infarction in dogs.

We studied 25 anesthetized and thoracotamized dogs before and during 5 hours of acute regional myocardial ischemia. Krypton-81m (81mKr) was infused constantly into the aortic sinuses. The myocardial equilibrium of this tracer was used to image and assess the distribution of regional myocardial perfusion using a gamma camera and digital computer. The epicardial ECG was recorded, S-T segment elevation and the loss of R and appearance of Q waves were measured, and the plasma activity of creatine kinase (CK) was determined in aortic and coronary venous blood throughout these experiments. Ten dogs underwent left anterior descending coronary artery (LAD) narrowing for 5 hours and received no drugs. Five dogs received nifedipine 13 microgram/kg, and another five received 1.0 microgram/kg intravenously 30 minutes after LAD narrowing. Those dogs receiving nifedipine, 13 microgram/kg, showed a 30% fall in aortic pressure, a 12% rise in heart rate, and an extension of regional ischemia. The ECG showed an extension of infarct size, and CK release into the coronary vein appeared earlier than in the controls. Dogs receiving nifedipine, 1 microgram/kg, showed a 12% fall in blood pressure, no rise in heart rate, an improvement in regional perfusion, and ECG signs that suggested limitation of infarct size. There also was delayed release of coronary venous CK. The effects of nifedipine on the natural history of regional myocardial perfusion, the electrocardiogram, and enzyme release from the heart were dose related and cannot be generalized. These observations warrant further clinical investigation to improve the use of this agent in man.

Animals↗

Abnormal ventilation scans in middle-aged smokers. Comparison with tests of overall lung function.

We have assessed the uniformity of regional ventilation during tidal breathing using continuous inhalation of krypton-81m in 43 male, lifelong nonsmokers and 46 male, current cigarette smokers (mean daily consumption 24.1 cigarettes/day) between 44 and 61 yr of age and with mild or no respiratory symptoms. All subjects had normal chest radiographs. The results of the ventilation scans were compared with tests of overall lung function (spirometry, maximal expiratory flow-volume curves, and single-breath N2 test). Diffuse abnormalities of the ventilation scan were found in 19 (41%) of the 46 smokers but in none of the nonsmokers. Focal abnormalities were found in 7 smokers and 3 nonsmokers. Smokers showed the expected abnormalities in overall lung function (reduced FEV1 and VC, increased single-breath N2 slope, and closing volume), but in individual smokers there was only a weak relation between the severity of abnormality of overall lung function and an abnormal ventilation scan. Abnormal scans could be found when overall lung function was normal and were not invariably found when significant abnormalities in FEV1/VC or N2 slope were present. There was no relation between the presence of chronic expectoration and an abnormal scan. The prognostic significance of an abnormal ventilation scan in such smokers remains to be established.

Adult↗

Clinical applications of radionuclide lung scanning in infants and children.

Krypton 81m ventilation and technetium 99m perfusion lung scans in anterior, posterior and oblique views in 86 children (age range 14 days to 15 years) with various paediatric problems were obtained. On reviewing these studies we found four main areas of clinical usefulness. (a) Establishing the diagnosis; in a relatively small number of patients the lung scan was essential for either establishing the exact diagnosis or directing attention to the abnormal area. (b) Refuting a diagnosis: the two main groups in this category include possible bronchiectasis and inhaled foreign body. (c) Establishing the extent of the disease; radionuclide studies enable one to assess and follow up the extent of the disease in children with lower respiratory problems; a lung scan may obviate the need for bronchography in bronchiectatics failing to respond to medical treatment and for whom surgery is being considered. Repeat studies are useful in following the natural history or the response to treatment of various lung conditions. (d) Assessing the success of surgical procedures on the heart and on abnormal pulmonary arteries. We indicate that 81Krm ventilation/99Tcm perfusion scanning are particularly useful in small children in whom tests of overall pulmonary function cannot be carried out because of lact of co-operation.

Adolescent↗

Ventilation perfusion ratios of the normal supine lung using emission tomography.

Ventilation perfusion imaging using single photon emission tomography was carried out in the supine position on four subjects, three normal volunteers and one patient, all free from evidence of respiratory disease. Ventilation imaging was by means of the steady-state inhalation of krypton 81m, perfusion imaging utilised technetium 99m labelled macroaggregates. In the three normal volunteers, perfusion studies were repeated using steady state infusion of 81Krm in solution. Ventilation perfusion ratios were calculated for the anterior, middle and posterior regions of the lung using axial slices from apex to base. The results using 99Tcm for perfusion showed a gradient of ventilation perfusion ratios from front to back of 1.27 anteriorly and 0.93 posteriorly for the right lung, 1.06 anteriorly and 0.77 posteriorly for the left lung. Using 81Krm for perfusion a small gradient of 0.94 to 0.82 was observed.

Adult↗

Late pulmonary scintigraphic defects after uneventful recovery from simple focal pneumonia of childhood.

We present a pilot study designed to determine whether pneumonia in children accompanied by focal consolidation on the chest radiograph (CXR) is associated with late abnormality as detected by radionuclide lung scintigraphy. 14 children with focal pneumonia were assessed 1 year after initial presentation. In 12 both krypton-81m ventilation and technetium-99m MAA perfusion studies were performed, while in two only perfusion studies were obtained. All cases had a further CXR. Abnormal or equivocal cases were reviewed clinically. Scans and CXRs were independently assessed by two observers and subsequently consensus reached by discussion. There were five abnormal and nine normal lung scans. Two defects matched the site of the initial pneumonia while three did not. A CXR at the time of lung scanning was normal except for one case with a small focus of consolidation on the opposite side to the initial pneumonia but with a normal lung scan. Similarly, clinical assessment at the time of scanning was unremarkable, with no history of further respiratory episodes except in one child excluded from the study because of an interval diagnosis of asthma. This pilot study indicates that lung scan defects are common in the medium term after supposedly uncomplicated pneumonia in childhood. The cause of these defects remains uncertain. The correlation with the site of the initial radiological abnormality is variable, pointing to the need for further research in this area. The importance of independent blind interpretation is also highlighted.

Child↗

Effect of high-pressure gas on yeast growth.

Microcalorimetry is a useful tool for monitoring the growth behavior of microorganisms. In this study, microcalorimetry was used to investigate the effects of nitrogen, air, oxygen, nitrous oxide, argon, and krypton at high pressure on the growth of the yeast Saccharomyces cerevisiae. Growth thermograms (metabolic heat vs. incubation time) were generated to estimate metabolic activity under compressed gases and to determine the 50% inhibitory pressure (IP(50)) and minimum inhibitory pressure (MIP), which are regarded as indices of the toxicity of compressed gases. Based on MIP values, the most toxic to the least toxic gases were found to be: O(2) > N(2)O > air > Kr > N(2) > Ar.

Air Pressure↗