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Evidence supporting an increased presence of reactive oxygen species in the diseased equine joint.

Reactive oxygen species (ROS) are capable of degrading many components of the joint in the presence of insufficient antioxidant defences, and as a result have been implicated in the pathogenesis of joint disease in horses. However, to our knowledge, evidence of ROS occurring in diseased joints of horses has not been reported. The objective of this experiment was to compare differences in synovial fluid protein carbonyl content (as a marker of oxidative modification of synovial fluid proteins by ROS) and the antioxidant status of synovial fluid between clinically normal and diseased equine joints. Synovial fluid was collected from the metacarpophalangeal, metatarsophalangeal, carpal and tarsal joints of 4 horses, age 2-5 years, as controls, and from diseased joints (metacarpophalangeal, metatarsophalangeal, carpal, tarsal and/or femoropatellar) of 61 horses, age 2-5 years. Synovial fluid protein carbonyl content was higher (P<0.01) in diseased joints as compared to controls. Antioxidant status of synovial fluid from diseased joints was higher, but not significantly, than that of controls (P = 0.0595). These findings require further study to determine their contribution to the overall disease process.

Animals↗

The role of imprint cytology in diagnosis of various bone and joint diseases.

Using imprint cytodiagnosis on 63 cases of bone and joint lesions, there was only one false negative result, with ten unsatisfactory smear preparations giving an overall accuracy of 82.25 per cent. Imprint cytodiagnosis is simple, cheap, rapid easy to perform and interpret because of its better cytomorphological evaluation. The accuracy rate is high when used in conjunction with various cytochemical stains.

Bone Diseases↗

[Typical nuclear spin tomographic intensity distribution patterns in different knee joint diseases using a gradient echo sequence].

The knee joints of 31 patients and 3 volunteers were examined with a 1.5 T Magnetom using 3D-gradient echo sequences. We measured for the most widespread diseases: the gonarthrosis (n = 23) and rheumatoid arthritis (n = 8) the signal intensities in all joint parts and the typical signal plots were worked out. In case of minimal changes at the articular cartilage, the method developed and tried out by us can facilitate diagnosis. A special aspect of this method is also the patient follow-up which is now possible quantitatively and objectively.

Adult↗

Clinical application of arthroscopy in the temporomandibular joint diseases.

The author presents the methodology and findings of a procedure for arthroscopic examination of the temporomandibular joint cavity by means of a method of puncture of the temporomandibular joint which he developed. The procedure was based on the temporomandibular joint puncture method and involved the tandem insertion of a puncture needle and sheathed needle into the joint cavity and examination of the cavity and cavity wall under perfusion of physiological saline solution. Color photographs of the findings are presented. The advantages by this method are as follows: 1) Direct observation of the cavity, 2) observation of the movement of the joint, 3) biopsy for histopathologic diagnosis, 4) still and cine photography of the findings. Arthroscopy of the temporomandibular joint is at present being applied clinically and is extremely effective in the diagnosis and selection of therapy. It will not doubt play an important role in the diagnosis of the diseases of the temporomandibular joint in the future.

Adolescent↗