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Assessment of wool waste and hair waste as soil amendment and nutrient source.

A field and two container experiments were conducted to assess uncomposted wool and hair wastes as a nutrient source for crops and to evaluate their potential to improve soil biological and chemical properties. Overall, addition of wool or hair waste to soil increased yields of basil (Ocimum basilicum L. 'Trakia'), thorn apple (Datura innoxia Mill. 'Inka'), peppermint (Mentha x piperita L. 'Black Mitchum'), and garden sage (Salvia officinalis L. 'Desislava'), increased NH(4)-N and NO(3)-N in soil, increased total N (and protein) content in plant tissue, stimulated soil microbial biomass, and decreased mycorrhizae colonization of plant roots of thorn apple but not in basil. Wool and hair waste additions to soil altered slightly the content and composition of plant secondary metabolites (essential oils or alkaloids); however, overall the constituents remained within the "typical" range for the respective crops. Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis demonstrated that wool and hair wastes decompose slowly under field or greenhouse conditions, and act as a slow release S, N, P, and K fertilizer. These results, along with the measured concentrations of NO(3)-N in soil at harvest, suggest that the addition of wool or hair waste of only 3.3 g kg(-1) of soil may support two to five harvests or crops under greenhouse conditions and two to four field seasons in field production systems, and would improve soil biological and chemical characteristics. Further research is needed to optimize the rate of application of these waste materials to the nutrient requirements of specific crops to avoid nitrate leaching into the ground water. In addition, the effect of wool and hair waste on other environmental end points should also be further investigated before specific recommendations for growers are provided.

Absorptiometry, Photon↗

Geostatistics, remote sensing and precision farming.

Precision farming is possible today because of advances in farming technology, procedures for mapping and interpolating spatial patterns, and geographic information systems for overlaying and interpreting several soil, landscape and crop attributes. The key component of precision farming is the map showing spatial patterns in field characteristics. Obtaining information for this map is often achieved by soil sampling. This approach, however, can be cost-prohibitive for grain crops. Soil sampling strategies can be simplified by use of auxiliary data provided by satellite or aerial photo imagery. This paper describes geostatistical methods for estimating spatial patterns in soil organic matter, soil test phosphorus and wheat grain yield from a combination of Thematic Mapper imaging and soil sampling.

Agriculture↗

Comments on "Bioprocessing in space"

An analysis developed by Westgate et al. for the digestible energy of edible and inedible biomass, including hydrolysis and fermentation, is reexamined with state-of-the-art values for the harvest index of hydroponic crops.

Biomass↗

Powdery mildew susceptibility and biotrophic infection strategies.

Plants are resistant to most potentially pathogenic microbes. This forces plant pathogens to develop sophisticated strategies to overcome basic plant resistance, either by masking intrusion or by suppression of host defences. This is particularly true for fungal pathogens, which establish long lasting interactions with living host tissue, without causing visible damage to invaded cells. The interactions of cereal crops and Arabidopsis with powdery mildew fungi are model systems for understanding host resistance. Currently, these systems are also promoting the understanding of fungal infection by identifying fungal pathogenicity and virulence factors and host target sites. This minireview focuses on recent findings about host susceptibility and the way powdery mildew fungi might induce it.

Apoptosis↗

Influence of atmospheric pollutants on agriculture in developing countries: a case study with three new wheat varieties in Pakistan.

The performance of three wheat varieties (Inqilab-91, Punjab-96, Pasban-90) was investigated in open-top chambers with charcoal-filtered air (FA), unfiltered air (UFA) and unchambered field plots (AA) during the 2003-2004 season at a semi-urban site in Lahore, Pakistan. The 8-h daily mean O(3), NO(2) and SO(2) concentrations in UFA and AA remained 72, 28 and 15 ppb, respectively. Plants grown in UFA and AA showed stunted growth and accelerated rate of leaf senescence with pronounced reductions in stomatal conductance (7-24%), net photosynthetic rate (20-22%) and photosynthetic efficiency (9-17%) compared with FA plants. A mid-season harvest of 10-week-old plants revealed reduced plant fresh (14-37%) and dry weights (15-43) in UFA compared to FA counterparts. Grain yield recorded after a full season of growth was drastically reduced in UFA with 43% for Pasban-90, 39% for Punjab-91 and 18% for Inqilab-91 compared with FA control plants. Nutritional quality of seeds was also significantly reduced in UFA and AA with respect to starch, but not in protein and vitamin-E contents when compared with FA plants. This investigation carried out in a major developing country of southeast Asia has shown alarming effects of atmospheric pollutants on both yield and nutritional quality of an important cereal crop that needs urgent attention to maintain the agricultural systems with parallel efforts to reduce the emissions in the region.

Agriculture↗

A silicon transporter in rice.

Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging (falling over) and increasing resistance to pests and diseases, as well as other stresses. Silicon is essential for high and sustainable production of rice, but the molecular mechanism responsible for the uptake of silicon is unknown. Here we describe the Low silicon rice 1 (Lsi1) gene, which controls silicon accumulation in rice, a typical silicon-accumulating plant. This gene belongs to the aquaporin family and is constitutively expressed in the roots. Lsi1 is localized on the plasma membrane of the distal side of both exodermis and endodermis cells, where casparian strips are located. Suppression of Lsi1 expression resulted in reduced silicon uptake. Furthermore, expression of Lsi1 in Xenopus oocytes showed transport activity for silicon only. The identification of a silicon transporter provides both an insight into the silicon uptake system in plants, and a new strategy for producing crops with high resistance to multiple stresses by genetic modification of the root's silicon uptake capacity.

Amino Acid Sequence↗

The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley.

Fungi of the recently defined order Sebacinales (Basidiomycota) are involved in a wide spectrum of mutualistic symbioses (including mycorrhizae) with various plants, thereby exhibiting a unique potential for biocontrol strategies. The axenically cultivable root endophyte Piriformospora indica is a model organism of this fungal order. It is able to increase biomass and grain yield of crop plants. In barley, the endophyte induces local and systemic resistance to fungal diseases and to abiotic stress. To elucidate the lifestyle of P. indica, we analyzed its symbiotic interaction and endophytic development in barley roots. We found that fungal colonization increases with root tissue maturation. The root tip meristem showed no colonization, and the elongation zone showed mainly intercellular colonization. In contrast, the differentiation zone was heavily infested by inter- and intracellular hyphae and intracellular chlamydospores. The majority of hyphae were present in dead rhizodermal and cortical cells that became completely filled with chlamydospores. In some cases, hyphae penetrated cells and built a meshwork around plasmolyzed protoplasts, suggesting that the fungus either actively kills cells or senses cells undergoing endogenous programmed cell death. Seven days after inoculation, expression of barley BAX inhibitor-1 (HvBI-1), a gene capable of inhibiting plant cell death, was attenuated. Consistently, fungal proliferation was strongly inhibited in transgenic barley overexpressing GFP-tagged HvBI-1, which shows that P. indica requires host cell death for proliferation in differentiated barley roots. We suggest that the endophyte interferes with the host cell death program to form a mutualistic interaction with plants.

Apoptosis↗

Antibodies: an alternative for antibiotics?

In 1967, the success of vaccination programs, combined with the seemingly unstoppable triumph of antibiotics, prompted the US Surgeon General to declare that "it was time to close the books on infectious diseases." We now know that the prediction was overly optimistic and that the fight against infectious diseases is here to stay. During the last 20 yr, infectious diseases have indeed made a staggering comeback for a variety of reasons, including resistance against existing antibiotics. As a consequence, several alternatives to antibiotics are currently being considered or reconsidered. Passive immunization (i.e., the administration of more or less pathogen-specific antibodies to the patient) prior to or after exposure to the disease-causing agent is one of those alternative strategies that was almost entirely abandoned with the introduction of chemical antibiotics but that is now gaining interest again. This review will discuss the early successes and limitations of passive immunization, formerly referred to as "serum therapy," the current use of antibody administration for prophylaxis or treatment of infectious diseases in agriculture, and, finally, recent developments in the field of antibody engineering and "molecular farming" of antibodies in various expression systems. Especially the potential of producing therapeutic antibodies in crops that are routine dietary components of farm animals, such as corn and soy beans, seems to hold promise for future application in the fight against infectious diseases.

Animal Diseases↗

Insect pests of pigeonpea and their management.

Pigeonpea (Cajanus cajan) is an important crop in semi-arid tropical and subtropical farming systems, providing high quality vegetable protein, animal feed, and firewood. Insect pests feeding on flowers, pods, and seeds are the most important biotic constraint affecting pigeonpea yields. This review summarizes the biology and ecology of the three most important groups of pests: flower- and pod-feeding Lepidoptera, pod-sucking Hemiptera, and seed-feeding Diptera and Hymenoptera. Recent research investigating the complex interactions among pigeonpea, its key pests, and their natural enemies is also reviewed. These relationships have implications on the pest status of individual species and on possible control strategies. Pigeonpea pest management research has focused until recently on the identification and development of resistant cultivars and on chemical control. Future research must focus on environmentally sound pest management strategies that are compatible with the needs and limitations of pigeonpea farmers. Several priority areas for research are suggested.

Journal Article↗

Invasion biology of thrips.

Thrips are among the stealthiest of insect invaders due to their small size and cryptic habits. Many invasive thrips are notorious for causing extensive crop damage, vectoring viral diseases, and permanently destabilizing IPM systems owing to irruptive outbreaks that require remediation with insecticides, leading to the development of insecticide resistance. Several challenges surface when attempting to manage incursive thrips species. Foremost among these is early recognition, followed by rapid and accurate identification of emergent pest species, elucidation of the region of origin, development of a management program, and the closing of conduits for global movement of thrips. In this review, we examine factors facilitating invasion by thrips, damage caused by these insects, pre- and post-invasion management tactics, and challenges looming on the horizon posed by invasive Thysanoptera, which continually challenge the development of sustainable management practices.

Animals↗

Water-quality effects of incorporating poultry litter into perennial grassland soils.

Poultry litter provides a rich source of nutrients for perennial forages, but the usual practice of surface-applying litter to pastures can degrade water quality by allowing nutrients to be transported from fields in surface runoff, while much of the NH4-N volatilizes. Incorporating litter into the soil can minimize such problems in tilled systems, but has not been used for perennial forage systems. In this study, we minimized disturbance of the crop, thatch, and soil structure by using a knifing technique to move litter into the root zone. Our objective was to determine effects of poultry litter incorporation on quantity and quality of runoff water. Field plots were constructed on a silt loam soil with well-established bermudagrass [Cynodon dactylon (L.) Pers.] and mixed grass forage. Each plot had 8 to 10% slopes, borders to isolate runoff, and a downslope trough with sampling pit. Poultry litter was applied (5.6 Mg ha(-1)) by one of three methods: surface-applied, incorporated, or surface-applied on soil-aeration cuts. There were six treatment replications and three controls (no litter). Nutrient concentrations and mass losses in runoff from incorporated litter were significantly lower (generally 80-95% less) than in runoff from surface-applied litter. By the second year of treatment, litter-incorporated soils had greater rain infiltration rates, water-holding capacities, and sediment retention than soils receiving surface-applied litter. Litter incorporation also showed a strong tendency to increase forage yield.

Animals↗

Root growth promotion by Penicillium melinii : mechanistic insights and agricultural applications.

This study characterizes Penicillium melinii , an endophytic fungus isolated from Arabidopsis thaliana roots, as a plant growth-promoting fungus with potential use as a model to study root development and as a biostimulant for sustainable agriculture. Although endophytes are known to promote plant growth, the underlying molecular mechanisms often remain poorly understood. Here, we aimed to elucidate how P. melinii enhances root system development and to assess its applicability across different crops. Phenotypic assays were conducted in Arabidopsis, quinoa and tomato under in vitro , greenhouse and field conditions. Root architecture and biomass were quantified using image-based phenotyping. Transcriptomic and phytohormone profiling assessed plant responses, and fungal genome sequencing coupled with secretome analysis was used to identify candidate effectors and metabolic traits. P. melinii consistently promoted root growth and increased plant biomass across species and environments, both in vitro and in the greenhouse. In tomato field trials, this translated into a significant increase in yield. The fungus colonized root surfaces without vascular penetration and triggered a mild transcriptomic response: early activation of stress-response genes followed by their attenuation and sustained upregulation of auxin-related pathways. Notably, the interaction modulates the SLR-ARF-LBD pathway and the number of pre-branch sites probably through increased auxin signalling in the oscillation zone. Additional hormonal changes were limited and mainly associated with the attenuation of the plant response to microorganisms. P. melinii enhances lateral root formation through a subtle molecular and metabolic dialogue with the host plant, underscoring its relevance as a model for studying root developmental plasticity. Its strong and reproducible growth-promoting effect, demonstrated with different fungal strains and under controlled and field conditions, supports its potential as a biostimulant for sustainable crop production.

Journal Article↗

[Choice of plant light status for space greenhouse: results of ground-based experience].

To decide on the light status of plants in space greenhouse, a theoretical study was undertaken to correlate specific productivity of space greenhouse with illumination characteristics including vertical PAR flux density (I), photoperiod (tau), and crop leaf index (L). It was demonstrated that in pace with I the daily productivity per a volume unit tended to monotonously approach maximum at I = Ip, whereas the greenhouse energy efficiency ME peaked at I = IE, IK < IE < IP, where IK is a compensation point of the light curve of crop photosynthesis. Proposed are compromise criteria to optimize illumination as a maximum of linear combination of MV and ME and coefficients which account for the cost of a space station volume unit and a unit of board power supply, and as maximum of product Q = MV.ME. Experimental results serve as the basis for a technique for determination of the best, by the Q criterion, light status parameters for three types of space greenhouses: research growth chamber for synchronous cultivation of leaf mustard, wheat growth chamber with fixed crop density, and green conveyer for cultivation of Brassica pekinensis (Lour Rupor). For the last mentioned Q effective I and tau values differed with the conveyer step. The technique allows design of ground-based experiments aimed at determination of the most effective light status of space-grown crops.

Brassica↗

Intensive groundwater use: a silent revolution that cannot be ignored.

Over the last decades, agriculture in arid and semi-arid countries has experienced a true "silent revolution" of intensive groundwater use. Millions of independent farmers worldwide have chosen to become increasingly dependent on the reliability of groundwater resources, and as a result their countries have reaped abundant social and economic benefits. Data from several countries shows that groundwater irrigation presents a much greater efficiency, than surface water irrigation systems, thus contributing to fulfil the motto of "more crops and jobs per drop". If this situation is confirmed globally, the usual world water visions have to be reviewed. However, the "silent revolution" has been carried out with scarce control on the part of governmental water agencies, and thus a series of unwanted effects have developed in certain places. While these by no means justify the pervasive "hydromyths" and obsolete paradigms that voice the frailty of groundwater, appropriate management of groundwater resources remains a worldwide challenge. This paper provides an overview of these issues, and concludes with the necessity there is to educate all levels of society on the importance of groundwater and to create bottom-up user associations to manage aquifers as common pool resources.

Agriculture↗

[Mechanism of antimutagenic activity of Pseudomonas syringae lipopolysaccharides].

The antimutagenic activity of lipopolysaccharides of phytopathogenic bacteria,which are the agents of grain crops diseases, has been investigated using bacterial test-system with Salmonella typhimurium. It has been established that the greatest antimutagen activity of Pseudomonas syringae pv. atrofaciens and P. syringae pv. coronafaciens lipopolysaccharides was,if they and bichromate potassium be simultaneously added to cells test-strains of S. typhimurium. They decreased to a less degree quantity of His+ -revertants in S. typhimurium cells pretreated by potassium bichromate. Li popolysaccharides of pathovars of P. syringae do not increase test-strain cells resistance to mutagenic effect of potassium bichromate under pretreatment of S. typhimurium by them.

Antimutagenic Agents↗

Cyclic nucleotides and the control of epithelial cell proliferation: cyclic CMP may be a partial mediator of the response of the pigeon crop-sac to prolactin.

The possible role of cyclic nucleotides as second messengers mediating hormone-induced cellular growth in vivo was investigated using the proliferative response of the pigeon crop-sac mucosal epithelium to prolactin (PRL) as a model system. Local injections of cAMP, cCMP, cGMP, cTMP or cUMP alone over the prolactin-responsive cells had no mitogenic effect. When injected along with a small dose of PRL, cAMP at doses above 1 micromole inhibited the response by 10%. While cGMP at a dose of 10 micromoles augmented the response by 47%. In fact, cCMP gave a log-linear dose-response relationship with significant augmentation of the response to PRL observed with doses as low as 0.01 micromole. Cyclic UMP, cTMP and various 5'-nucleotide monophosphates had no effect on the response to the hormone. When the dibutyryl analogs of the cyclic nucleotides were tested for their ability to potentiate the response to PRL, only dibutyryl cCMP was effective. These data suggest that cCMP may be a partial mediator of the proliferative action of PRL in this system, but it is probably not the sole second messenger for prolactin's action in these cells. Our results also indicate that failure of a cyclic nucleotide to mimic a hormone-induced response by itself does not prove that the compound is uninvolved in the hormones's action.

Animals↗

Starch grains reveal early root crop horticulture in the Panamanian tropical forest.

Native American populations are known to have cultivated a large number of plants and domesticated them for their starch-rich underground organs. Suggestions that the likely source of many of these crops, the tropical forest, was an early and influential centre of plant husbandry have long been controversial because the organic remains of roots and tubers are poorly preserved in archaeological sediments from the humid tropics. Here we report the occurrence of starch grains identifiable as manioc (Manihot esculenta Crantz), yams (Dioscorea sp.) and arrowroot (Maranta arundinacea L.) on assemblages of plant milling stones from preceramic horizons at the Aguadulce Shelter, Panama, dated between 7,000 and 5,000 years before present (BP). The artefacts also contain maize starch (Zea mays L.), indicating that early horticultural systems in this region were mixtures of root and seed crops. The data provide the earliest direct evidence for root crop cultivation in the Americas, and support an ancient and independent emergence of plant domestication in the lowland Neotropical forest.

Agriculture↗

Signal crosstalk and induced resistance: straddling the line between cost and benefit.

This review discusses recent progress in our understanding of signaling in induced plant resistance and susceptibility to pathogens and insect herbivores, with a focus on the connections and crosstalk among phytohormone signaling networks that regulate responses to these and other stresses. Multiple stresses, often simultaneous, reduce growth and yield in plants. However, prior challenge by a pathogen or insect herbivore also can induce resistance to subsequent challenge. This resistance, or failure of susceptibility, must be orchestrated within a larger physiological context that is strongly influenced by other biotic agents and by abiotic stresses such as inadequate light, temperature extremes, drought, nutrient limitation, and soil salinity. Continued research in this area is predicated on the notion that effective utilization of induced resistance in crop protection will require a functional understanding of the physiological consequences of the "induced" state of the plant, coupled with the knowledge of the specificity and compatibility of the signaling systems leading to this state. This information may guide related strategies to improve crop performance in suboptimal environments, and define the limits of induced resistance in certain agricultural contexts.

Agriculture↗