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Medical management of the traumatic consequences of civil unrest incidents: causation, clinical approaches, needs and advanced planning criteria.

In the context of this review, civil unrest is defined as disharmony, expressive dissatisfaction and/or disagreement between members of a community, which leads to a situation of competitive aggression that may find expression as disruption of organisation, conflicts, damage to property and injuries. Such a breakdown of harmonious relationships, which may result in property damage and human injuries that may be threatening to life, varies in magnitude from participation of a very few individuals up to the involvement of large crowds of people, which may evolve into a full-scale riot. It is the latter situation often involving demonstrators, opposing groups and law enforcement personnel that can result in multiple casualties and present a very significant challenge to the resources of local healthcare institutions. The causation of civil unrest incidents is multifactorial and has generic, specific and potentiating elements. With the current national and international societal, political and discriminatory problems, it is likely that civil unrest incidents on both small and large scales will continue to occur at a high and possibly increasing rate on a worldwide basis, and for these not infrequent incidents, the medical community should be in a state of informed preparation. The circumstances of civil unrest incidents are very variable with respect to causation, overall magnitude, frequency, timing, geographical location, numbers of persons involved, demographics of participants, influence of extremists, confrontation with opposing groups and control measures used by law enforcement agencies. Methods used by police and security forces for the control of civil unrest incidents, if advanced negotiations with organisers and verbal warnings have failed, fall basically into two categories: physical and chemical measures. Physical methods include restraint holds, truncheons, batons, mounted horses, projectiles (such as bean bags, plastic and rubber bullets), water cannons, tasers and (rarely) live ammunition. All of these physical measures are associated with pain and immobilisation, and there is a high potential for soft tissue and bone injuries. Some of the more severe physical methods, including plastic and rubber bullets, may cause lethal injuries. The basis for using chemicals in civil unrest incidents is that they cause distraction, transient harassment and incapacitation, temporary impairment of the conduct of coordinated tasks and cause a desire to vacate the area of unrest. Although screening smokes and malodors have sometimes been employed, the major group of chemicals used are peripheral chemosensory irritants (PCSIs), which reversibly interact with sensory nerve receptors in exposed skin and mucosal surfaces, resulting in the production of local uncomfortable sensations and associated reflexes. Major effects are on the eye, respiratory tract and (to a lesser degree) skin. Thus, the induced transient pain and discomfort in the eye, respiratory tract and skin, together with associated lacrimation, blepharospasm, rhinorrhoea, sialorrhoea, cough and breathing difficulties, produce temporary incapacitation and interference with the conduct of coordinated tasks, and form the basis for harassment of malefactors. Currently used peripheral chemosensory irritants are 1-chloroacetophenone, 2-chlorobenzylidene malononitrile, dibenz(b.f)-1,4-oxazepine, oleoresin capsicum and pelargonic acid vanillylamide. Depending on operational circumstances, irritants may be dispersed as a smoke, powder cloud, aerosol, vapour, or in solution; the mode of generation and dispersion of irritant can influence hazard. Brief acute exposure to chemosensory irritants produces effects that generally resolve within an hour, leaving no long-term sequelae. However, sustained exposure to high concentrations may produce tissue injury, notably to the eye, respiratory tract and skin. With solutions of sensory irritants, other formulation constituents may enhance PCSI toxicity or introduce additional local and/or systemic toxicity. By the very circumstances of civil unrest incidents, injuries are inevitable, particularly when emotions are heightened and police and security forces have to resort to various chemical and/or physical means of control. Trauma may include slight to severe physical and/or chemical injuries, psychological problems and occasional deaths. Hospitals should be prepared for a wide range of casualties, and the fact that those seeking help will constitute a heterogeneous group, including wide age range, male, female, and individuals with pre-existing ill health. A major civil unrest incident necessitates that the local receiving hospital should be prepared and equipped for decontamination and triage processes. It is necessary to reassure patients who have been exposed to sensory irritants that the signs and symptoms are rapidly reversible, and do not result in long-term sequelae. With respect to chemical exposures, detailed evaluation should be given to possible ocular, cutaneous, respiratory and gastrointestinal effects. Also, exposure to chemosensory irritants results in transient increases in blood pressure, bradycardia and increased intraocular pressure. This indicates that those with cardiovascular diseases and glaucoma may be at increased risk for the development of complications. This article details the pharmacological, toxicological and clinical effects of chemicals used in civil disturbance control and discusses the management of contaminated individuals. Additionally, the potential for adverse effects from delivery systems and other physical restraint procedures is summarised. Due to the emergency and specialised circumstances and conditions of a civil unrest incident, there is a clear need for advanced planning by healthcare institutions in the event that such an incident occurs in their catchment area. This should include ensuring a good information base, preparations for medical and support staff readiness, and availability of required equipment and medications. Ideally, planning, administration and coordination should be undertaken at both local (regional) and central (governmental) centres. Regional centres should have responsibilities for education, training, ensuring facilities and staffing are appropriate, and that adequate equipment and medicines are available. There should be cooperative interactions and communications with local police and other emergency services. Centrally directed functions should include ensuring adequacy of the information base, coordinating activities and agreeing approaches between the regional centres, and periodic audits of regional centres with respect to the staffing, facility, equipment and training needs. Also, there is a need for most countries to introduce detailed guidelines and formal (regulatory) schemes for the assessment of the safety-in-use of chemicals and the delivery systems that are to be used against heterogeneous human populations for the control of civil unrest incidents. Such regulatory approval schemes should also cover advisory functions for safe use and any required restrictions.

Civil Defense↗

Topical capsaicin in painful diabetic neuropathy. Controlled study with long-term follow-up.

OBJECTIVE: We conducted an 8-wk controlled study with topical 0.075% capsaicin in subjects with chronic severe painful diabetic neuropathy who were unresponsive or intolerant to conventional therapy. Capsaicin is an alkaloid found in capsicum peppers and produces desensitization to noxious thermal, chemical, and mechanical stimuli when applied topically. RESEARCH DESIGN AND METHODS: In 22 randomly assigned subjects, either capsaicin or vehicle cream was applied to painful areas 4 times/day. Pain measurements were recorded at baseline and at 2-wk intervals for 8 wk. RESULTS: Capsaicin treatment was more beneficial than vehicle treatment in the overall clinical improvement of pain status, as measured by physician's global evaluation (P = 0.038) and by a categorical pain severity scale (P = 0.057). Decrease in mean pain intensity by a visual analogue scale was 16% in capsaicin-treated and 4.1% in vehicle-treated subjects. Mean pain relief on visual analogue scale was 44.6 and 23.2%, respectively. In a follow-up open-label study, approximately 50% of subjects reported improved pain control or were cured, and 25% each were unchanged or worse. A burning sensation at the application site was noted by some subjects but both its magnitude and duration decreased with time. CONCLUSIONS: Results from this preliminary study suggest that topical 0.075% capsaicin may be of value in subjects with diabetic neuropathy and intractable pain.

Administration, Topical↗

Effect of plant extracts and formic acid on the intestinal equilibrium of early-weaned pigs.

We evaluated the effects of a plant extracts mixture (XT) standardized in 5% (wt/wt) carvacrol, 3% cinnamaldehyde, and 2% capsicum oleoresin (oregano, cinnamon and Mexican pepper), alone or in combination with formic acid (FA), on the productive performance and the intestinal ecosystem of the early-weaned pig. Pigs weaned at 20 +/- 1 d of age (n = 216) were allocated in 24 pens and fed a standard medicated prestarter diet for 12 d. Twelve days after weaning, a stress management system based on social and dietary stress factors was applied to the animals, after which, each group was allocated to one of six dietary treatments, which followed a factorial arrangement, with three levels (as-fed basis) of the XT (0, 150, and 300 mg/kg) and two levels of FA (0 and 0.5%). On d 24 and 25 after the stress episode, eight pigs per treatment were killed to examine variables describing some aspects of the gastrointestinal ecology. Two days after the stress episode, an Escherichia coli K88 diarrhea episode occurred, and five casualties were registered. Four of the five deaths occurred in pens of pigs not fed the XT. The FA resulted in better G:F (P = 0.040) in coincidence with shorter villous height (P = 0.073) and lower rectal total microbial mass (P = 0.078). Both XT and FA addition increased stomach content (P = 0.006 and 0.003, respectively) and percentage of DM (P = 0.089 and 0.010, respectively), suggesting an increased gastric retention time; consequently, pH was also increased (P = 0.005 and 0.060, respectively). The XT decreased ileum total microbial mass (P = 0.025) and increased the lactobacilli:enterobacteria ratio (P = 0.002). The VFA profile in the cecum and colon was modified by XT inclusion, increasing the proportion of acetate (P = 0.018 and 0.025, respectively) and diminishing the proportion of butyrate (P = 0.096 and 0.040, respectively) and valerate (P = 0.001 and 0.039, respectively). Both XT and FA were shown to be effective in modifying the gastrointestinal ecosystem, stomach contents, and stomach emptying rate, which are proposed as important aspects in the mechanisms of action for these additives.

Aging↗

Screening for the effects of natural plant extracts at different pH on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle.

Six natural plant extracts and three secondary plant metabolites were tested at five doses (0, 0.3, 3, 30, and 300 mg/L) and two different pH (7.0 and 5.5) in a duplicate 9 x 5 x 2 factorial arrangement of treatments to determine their effects on in vitro microbial fermentation using ruminal fluid from heifers fed a high-concentrate finishing diet. Treatments were extracts of garlic (GAR), cinnamon (CIN), yucca (YUC), anise (ANI), oregano (ORE), and capsicum (CAP) and pure cinnamaldehyde (CDH), anethole (ATL), and eugenol (EUG). Each treatment was tested in triplicate and in two periods. Fifty milliliters of a 1:1 ruminal fluid-to-buffer solution were introduced into polypropylene tubes supplied with 0.5 g of DM of a 10:90 forage:concentrate diet (15.4% CP, 16.0% NDF; DM basis) and incubated for 24 h at 39 degrees C. Samples were collected for ammonia N and VFA concentrations. The decrease in pH from 7.0 to 5.5 resulted in lower (P < 0.05) total VFA, ammonia N, branched-chain VFA concentration, acetate proportion, and acetate:propionate, and in a higher (P < 0.05) propionate proportion. The interaction between pH and doses was significant for all measurements, except for ATL and CDH for butyrate, ATL and EUG for acetate:propionate ratio, and ORE for ammonia N concentration. The high dose of all plant extracts decreased (P < 0.05) total VFA concentrations. When pH was 7.0, ATL, GAR, CAP, and CDH decreased (P < 0.05) total VFA concentration, and ANI, ORE, CIN, CAP, and CDH increased (P < 0.05) the acetate:propionate. The CIN, GAR, CAP, CDH, ORE, and YUC decreased (P < 0.05), and EUG, ANI, and ATL increased (P < 0.05) ammonia N concentration. The effects of plant extracts on the fermentation profile when pH was 7.0 were not favorable for beef production. In contrast, when pH was 5.5, total VFA concentration did not change (ATL, ANI, ORE, and CIN) or increased (P < 0.05) (EUG, GAR, CAP, CDH, and YUC), and the acetate:propionate (ORE, GAR, CAP, CDH, and YUC) decreased (P < 0.05), which would be favorable for beef production. Ammonia N (ATL, ANI, CIN, GAR, CAP, and CDH) and branched-chain VFA (ATL, EUG, ANI, ORE, CAP, and CDH) concentrations also were decreased (P < 0.05), suggesting that deamination was inhibited. Results indicate that the effects of plant extracts on ruminal fermentation in beef cattle diets may differ depending on ruminal pH. When pH was 5.5, GAR, CAP, YUC, and CDH altered ruminal microbial fermentation in favor of propionate, which is more energetically efficient.

Ammonia↗

Effects of spray-dried porcine plasma and plant extracts on intestinal morphology and on leukocyte cell subsets of weaned pigs.

We evaluated the effects of a 6% spray-dried porcine plasma (SDPP) and a plant extracts mixture (XT; 5% carvacrol, 3% cinnamaldehyde, and 2% capsicum oleoresin) on the productive performance, intestinal morphology, and leukocyte cell subsets of early-weaned pigs compared with a control group. Morphometry of the jejunum, ileum, and colon, and immune cell analysis of blood, ileocolic lymph node (LN), and ileal Peyer's patches were done in 24 weaned pigs (20 +/- 2 d) at 19 or 21 d postweaning. Although SDPP and XT treatments did not increase ADG or ADFI, SDPP improved the G:F ratio (P = 0.024) compared with the control group. Dietary SDPP reduced the percentages of blood monocytes (P = 0.006) and macrophages in ileal Peyer's patches and LN (P = 0.04), of B lymphocytes (P = 0.04) and gammadelta+ T cells in LN (P = 0.009), and of intraepithelial lymphocytes (P = 0.026) as well as the density of lamina propria cells in the colon (P < 0.01). Dietary XT reduced intraepithelial lymphocyte numbers in jejunum (P = 0.034) and the percentages of blood cytotoxic cells (P = 0.07) and B lymphocytes in LN (P = 0.03); however, XT increased blood monocytes (P = 0.038) and the density of lamina propria lymphocytes in the colon (P = 0.003). These results indicate that dietary SDPP and plant extracts can affect intestinal morphology and immune cell subsets of gut tissues and blood in weaned pigs. Furthermore, the effects of SDPP suggest lower activation of the immune system of the piglets.

Animal Feed↗

Chemoprevention of azoxymethane-induced rat colon carcinogenesis by dietary capsaicin and rotenone.

The modifying effects of dietary administration of capsaicin, which is the principal pungent capsicum fruit, and rotenone, which is a naturally occurring pesticide derived from Derris and Lonchorcarpus species, on azoxymethane (AOM)-induced colon tumorigenesis were investigated in male F344 rats. Gavage with capsaicin and rotenone significantly elevated phase II enzymes, glutathione S-transferase (GST) and quinone reductase (QR), in the liver and colon. In an aberrant crypt foci (ACF) bioassay, feeding of capsaicin and rotenone at a dose of 500 ppm for 4 weeks significantly inhibited ACF formation induced by AOM (20 mg/kg body weight, once a week for 2 weeks). In a subsequent long-term study designed to confirm the protective effects of both compounds on ACF development, one group was treated with AOM alone and four other groups received the carcinogen treatment plus diets containing 500 ppm test compounds for 4 weeks (initiation phase) and for 34 weeks (post-initiation phase). Two groups were treated with capsaicin or rotenone alone (500 ppm in diet) and one group was maintained on the basal diet. At the termination of the study, dietary exposure of capsaicin during the initiation phase was found to significantly reduce the incidence of colonic adenocarcinoma (60% vs. 24%, 60% reduction, P=0.0407). Rotenone feeding during the post-initiation phase also reduced the frequency of colonic adenocarcinoma (60% vs. 19%, 68% reduction, P=0.0226). Our results suggest that two natural compounds, capsaicin and rotenone, might be useful for the prevention of human colon cancers.

Adenocarcinoma↗

Comparison of solid and liquid forms of homeopathic remedies for tonsillitis.

The efficacy of three plants used in homeopathy to treat acute tonsillitis was evaluated. A fixed combination of three plant substances (Phytolacca americana, Guajacum officinale, Capsicum annuum) was used in either solid (tablet) or liquid (drop) formulation: 107 patients were treated and no antibiotics were used. The objective and subjective symptoms of acute tonsillitis were noted. A significant decrease in symptoms was observed as early as 2.5 days after treatment startup; no serious adverse effects were reported. It was concluded that acute tonsillitis can be treated with a homeopathic remedy characterized by its immunomodulatory, analgesic, and anti-inflammatory properties.

Acute Disease↗

Lack of the inhibitory effect of intragastrically administered capsaicin on NNK-induced lung tumor formation in the A.J mouse.

Capsaicin is the principal component in Capsicum fruits consumed by humans worldwide as a food additive. The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is thought to be an important causative factor in human lung cancer. Dietary factors are reported to modify lung tumor formation in laboratory animals and in humans. In this study, NNK-induced lung tumor formation in female A/J mice given intragastric doses of capsaicin (5 mg/kg body wt) was compared to mice not receiving capsaicin. At the end of the 21 week study, mice treated with capsaicin had an average of 17.1 +/- 1.8 lung tumors/mouse while untreated mice had 19.6 +/- 2. There were 100% lung tumor bearers in each group. Capsaicin alone did not affect spontaneous formation of lung tumors. Our results do not support a possible chemoprotective effect of dietary capsaicin toward NNK-induced lung tumors in human smokers.

Adenoma↗

Influence of intragastric perfusion of aqueous spice extracts on acid secretion in anesthetized albino rats.

BACKGROUND: The effect of spices on gastric acid secretion is variable. Their mechanism of action is also not well established. AIM: To study the effect of spices on gastric acid secretion in anesthetized rats. METHODS: Aqueous extracts (10% w/v) of red pepper (Capsicum annuum), fennel (Foeniculum vulgare), omum/ajwan (Carum copticum), cardamom (Elettaria cardamomum), black pepper (Piper nigrum), cumin (Cuminum cyminum) and coriander (Coriandrum sativum) were prepared. The stomach of pentobarbitone-anesthetized rats was perfused at 0.15 mL/min with aqueous extracts of spice or acetylcholine (1 microgram/mL or 10 micrograms/mL solutions, in 40 min blocks, twice in each experiment bracketed by saline perfusions. The acid content in the samples was estimated by titration with 0.1N NaOH with phenolphthalein as indicator. Atropine 1 microgram/mL was added to the perfusion fluid in 28 experiments. In 32, acute gastric mucosal injury was induced by leaving aspirin 125 mg/Kg in the stomach for 2 h before perfusion. RESULTS: All the spices tested increased acid secretion in the following declining order: red pepper, fennel, omum, cardamom, black pepper, cumin, coriander. Red pepper increased acid secretion (mean [SEM] 0.93 [0.16] mL 0.1N HCl) to about 7 times the basal secretion (0.14 [0.05]; p < 0.005). The increase in acid secretion by the other spices was as follows: fennel 0.42 (0.11) mL 0.1 N HCl from basal secretion (0.12 [0.03]) (p < 0.02); omum 0.33 (0.05) from 0.09 (0.02) (p < 0.01); cardamom 0.28 (0.04) from 0.10 (0.03) (p < 0.005); black pepper 0.19 (0.03) from 0.04 (0.01) (p < 0.005); cumin 0.12 (0.02) from 0.08 (0.01) (p < 0.05); coriander 0.18 (0.03) from 0.09 (0.02) (p < 0.005). Atropine abolished the acid secretion induced by acetylcholine and significantly reduced acid induction by red pepper, omum and coriander, but not that by fennel. In experiments with aspirin-induced mucosal injury the basal acid secretion was low; acid secretion by red pepper and fennel was reduced significantly, but not that by acetylcholine. Cumin and coriander increased acid secretion in injured stomachs. CONCLUSION: The spices tested increased gastric acid secretion, in some by a cholinergic mechanism but by other mechanism(s) as well. Red pepper produced maximum increase in acid secretion, but this was significantly reduced in injured stomachs. Cumin and coriander increased gastric secretion in injured stomachs.

Acetylcholine↗

Potential interactions between alternative therapies and warfarin.

Potential and documented interactions between alternative therapy agents and warfarin are discussed. An estimated one third of adults in the United States use alternative therapies, including herbs. A major safety concern is potential interactions of alternative medicine products with prescription medications. This issue is especially important with respect to drugs with narrow therapeutic indexes, such as warfarin. Herbal products that may potentially increase the risk of bleeding or potentiate the effects of warfarin therapy include angelica root, arnica flower, anise, asafoetida, bogbean, borage seed oil, bromelain, capsicum, celery, chamomile, clove, fenugreek, feverfew, garlic, ginger ginkgo, horse chestnut, licorice root, lovage root, meadowsweet, onion, parsley, passionflower herb, poplar, quassia, red clover, rue, sweet clover, turmeric, and willow bark. Products that have been associated with documented reports of potential interactions with warfarin include coenzyme Q10, danshen, devil's claw, dong quai, ginseng, green tea, papain, and vitamin E. Interpretation of the available information on herb-warfarin interactions is difficult because nearly all of it is based on in vitro data, animal studies, or individual case reports. More study is needed to confirm and assess the clinical significance of these potential interactions. There is evidence that a wide range of alternative therapy products have the potential to interact with warfarin. Pharmacists and other health care professionals should question all patients about use of alternative therapies and report documented interactions to FDA's MedWatch program.

Adult↗

Plant hydraulic conductance measured by the high pressure flow meter in crop plants.

A new high pressure flow meter (HPFM) method for measuring plant hydraulic conductances (K) was investigated to examine whether its results are comparable to those from a conventional evaporative flux (EF) method in crops. Hydraulic conductance (K) was measured by the two methods under quasi-steady-state conditions in six crops grown in pots: soybean (Glycine max L. Merr. cv. Tsurunoko daizu), sunflower (Helianthus annuus L. cv. Russian mammoth), kidney bean (Phaseolus vulgaris L. cv. Tsurunashi morocco), tomato (Lycopersicon esculentum Mill. cv. Sekai-ichi), green pepper (Capsicum annuum L. cv. shishitou), and eggplant (Solanum melongena L. cv. Seiguro chunaga nasu). There was a 1:1 agreement between K values measured by the two methods for K values of whole plant, root and stem, and leaf under quasi-steady-state conditions. Leaf water potential (psi leaf) and evaporative flux density (E) in sunflower was curvilinear, indicating whole plant K estimated by the EF method increased with increase of E. Predicted psi leaf (= E divided by whole plant K measured by the HPFM method) agreed with measured psi leaf. Diurnal changes were also found in K measured by the HPFM confirming that K changed in response to temperature and E. The HPFM revealed that variable conductance was located in all organs: roots, stems, petioles, and leaves. These observations indicated that the HPFM is valid for crops as well as for trees (as previously established by Tsuda and Tyree) and has advantages over the EF method because of the speed and ease of the HPFM method.

Biomechanical Phenomena↗

[Forensic medical aspects of injuries inflicted with self-defense capsaicin aerosols].

Toxicological and forensic medical characteristics of capsaicinoids are presented. Capsaicinoids represent a mixture of three basic compounds with similar structure: capsaicine, dihydrocapsaicine, and nordihydrocapsaicine, all of which are the primary components of red pepper extract (Oleoresin Capsicum). The advantages of this group of irritants in comparison with synthetic irritants are discussed. Probable routes of administration of the extract with regard to its hazard for the organism are considered. Results of experimental studies on nonbiological phantoms and volunteers are presented.

Aerosols↗

Cloning, expression, and biological activity of recombinant alpha-cinnamomin: toxicity to cranberry and other plant species.

Elicitins produced by the pathogenic fungi Phytophthora are known to exhibit The elicitin cinnamomin is of nonspecific toxicity to different solanaceous plant species. particular interest for its potential role in the hypersensitive-like cell death and in the biological response of cranberry plants to the fungal pathogen Phytophthora cinnamomi. In order to understand the biochemical steps of the Phytophthora root rot disease in cranberry, we investigated the alpha-cinnamomin-induced plant responses. Toxicity of alpha-cinnamomin, which shows a high degree of sequence homology to the alpha-elicitin group, was tested on Vaccinum macrocarpon, Nicotiana tabacum, Capsicum annuum, Lycopersicon esculentum, Lactuca sativa, and Phaseolus vulgaris plants. Gene corresponding to alpha-cinnamomin gene fused with maltose binding protein gene, was cloned into a pMALTEV expression vector, which was transformed into E. coli cells. Cells containing alpha-cinnamomin clones were cultured and extracted protein was purified on a maltose binding protein affinity column. Biological activity of alpha-cinnamomin fusion protein was examined on propagated plants and cuttings. In cranberry plants treated with cinnamomin necrotic hypersensitive-like response in the proximal areas of the leaf lamina of lower plant leaves was observed after 48-72 hr of incubation. Limited leaf necrosis observed days after application of low amounts of recombinant cinnamomin directly on the leaves of other plants indicates that the recombinant protein might be functioning as a toxin, capable of inducing aging accompanied by plant cell death.

Algal Proteins↗

[Influence of spiced feed additives on taste of hen's eggs].

The effect of the spices garlic (Allium sativum), extract from garlic, sage (Salvia officinalis), caraway (Carum carvi), peppermint (Mentha piperita), fennel (Foeniculum vulgare), thyme (Thymus vulgaris), paprika (Capsicum annuum), marjoram (Majorana hortensis), cardamom (Elettaria cardamomum) and sausage-mix fed to white hybrides on the quality of eggs (n = 705) was tested by 10 qualified persons. Changes in smell or taste were partly significant; a direct improvement is hard to induce. A well seasoned taste was obtained by feeding of garlic, fennel, peppermint and marjoram. They produce an aromatic flavor. A practical application is possible but limited by higher production prices. Further, it is uncertain if there is a need for "taste-manipulated" eggs.

Animal Feed↗

Nitrate pollution in groundwater and strategies to reduce pollution.

The input-intensive rainfed tropical ecosystem, where wet season (WS) rice (Oriza sativa L.)-dry season (DS) diversified high-value upland crops like vegetables predominate, has resulted in a problem of a large leakage of N into the environment, thereby polluting the water. Excessive use of N fertilizer in high-value crops grown in DS is economically motivated. Out of twenty water sources evaluated in a watershed with a total area of 265 ha located in Magnuang, Ilocos Norte, Philippines, twelve had near or above the World Health Organization's (WHO) NO3-N limit for drinking water of 10 ppm. Soil mineral N (upper 100 cm) observed in seven rice-sweet pepper (Capsicum annuum L.) farmers' fields ranged from 111 to 694 kg ha(-1) which decreased by 10 to 68% in plots with dry-to-wet (DTW) crops like indigo, indigo+mungo and corn. In fallow plots where mineral N was either maintained or increased, it showed movement to lower soil profiles demonstrating NO3 leaching without a crop. On average, maize (Zea mays L.) captured 176 kg N ha(-1) and indigo (Indigofera tinctoria L.) 194 kg N ha(-1). In both fallow and planted plots, mineral N declined to low levels at 100% water-filled pore spaces (WFPS) before rice transplanting. A strategy for including indigo plus maize as a N-catch crop is proposed to decrease NO3 leaching and maximize N use efficiency in a rice-sweet pepper cropping system.

Agriculture↗

[Phylogenetic analysis of chloroplast DNA of 18 Solanaceae species].

Chloroplast DNA of 18 Solanaceae species classified into 9 different tribes was analyzed using combined amplified fragment length polymorphism (AFLP) analysis. UPGMA phylogenetic tree was constructed based on the observed data set. In general, the conclusions of this study confirmed the morphology-based classification of these species. Cluster analysis has placed Mandragora into a monophiletic branch and grouped Schizantus and Capsicum into the independent clusters.

Base Sequence↗

Effect of aphid-infested plants on the host location and learning behaviour of the parasitoid Aphelinus abdominalis.

Aphelinus abdominalis Dalman (Hymenoptera: Aphelinidae) is a generalist parasitoid of cereal (e.g. Sitobion avenae) and greenhouse aphids (e.g. Macrosiphum euphorbiae). It differs from Aphidius spp. (Hymenoptera: Braconidae) by its robust constitution, longevity and its habit to use aphids not only as hosts for its progeny but also as prey (host feeding). In addition, A. abdominalis does not evoke Aphidius-characteristic defence responses by attacked aphids as this wasp approaches its hosts very carefully and then inserts its ovipositor nearly unnoticed by the aphids. In order to obtain more information on the host location behaviour of A. abdominalis, the effect of aphid-infested plants on its orientation and learning behaviour after mummy emergence was first studied in a series of Y-tube olfactometer experiments. When females reared on S. avenae were offered a 2 h foraging experience on M. euphorbiae-infested sweet pepper (Capsicum annuum) and then had the choice between the odour of uninfested sweet pepper plants and plants infested by aphid hosts (M. euphorbiae), they were significantly attracted to the odour of infested plants. Naive A. abdominalis, i.e. females that prior to the experiments were not offered any contact with a plant-host complex (PHC) after their emergence from S. avenae mummies and hence had no chance to learn the odour of the PHC, were not attracted by aphid-infested plants. The effects of adult experience on long-range host location by A. abdominalis (again reared on S. avenae) were then investigated in wind tunnel experiments by using M. euphorbiae-infested and uninfested sweet pepper and aubergine (Solanum melongena) plants. Females experienced with one of the offered PHCs significantly preferred the odour of the learnt PHC to that of the different PHC. A. abdominalis females obviously learn specific volatile signals emitted by aphid-infested plants for long-range host location.

Animals↗

[Determination of fluorine in the environmental samples by combustion-hydrolysis-ion selective electrode method].

OBJECTIVE: To establish a fast and accurately method of determination of fluorine in coal, hair and food by combustion-hydrolysis-ion selective electrode method. METHODS: The influence factors on combustion-hydrolysis, such as silica weight, temperature and time of hydrolysis, sample weight and volume of distilled water were studied so that to determine the best of all testing conditions. RESULTS: The time for hydrolysis in this method was reduced by one third of that of GB/T4633-1997 standard of PRC method. The fluorine in standard reference material such as tea, poplar leaves, coal and capsicum, and corn was determined on precision and accuracy by this method. The relative standard deviation was less than 5%. The recovery varied from 90.3% to 107.6%. CONCLUSION: The method is suitable for determining fluorine in the various environmental samples.

Carbon↗