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Body cell mass measured by total body potassium in normal-weight and obese men and women.

OBJECTIVE: Total body potassium (TBK) concentration is linearly correlated with the size of the body cell mass (BCM). The aim of this study was to compare BCM in normal-weight and obese individuals. METHODS: 271 individuals (207 males, 64 females), 20 to 67 years of age, participated in this study. Subjects were separated by body mass index (BMI): BMI < 25 kg/m(2) (BMI-L) and BMI > 25 kg/m(2) (BMI-H). (40)K was assessed using a whole-body counter and BCM was calculated. RESULTS: BCM and TBK were significantly greater in men, with a trend to be greater in women in BMI-H compared to men in BMI-L. TBK/body weight was significantly lower, while TBK/height was significantly greater for men and women in BMI-H compared to men and women in BMI-L. Fat-free mass (FFM) was significantly greater for men in BMI-H, with no significant differences in FFM between the two groups of women. CONCLUSIONS: The healthy obese subjects in the present study had a greater BCM than the non-obese subjects. These results indicate that it is important to assess BCM in obese individuals because it could influence the type of weight loss regimen that will be used in order to preserve BCM.

Adipose Tissue↗

Composition of body weight differences in subjects with the same body height.

OBJECTIVE: To determine the composition of body weight differences in men and women with the same height. DESIGN: A study of a random population sample of Danish subjects, aged 35-65 y. SUBJECTS: An age and sex stratified random sample of 3608 subjects of which 2987 (83%) subjects appeared for study. Of these, 548 men and 498 women were randomly selected for this present work based on their heights. RESULTS: The mean values of the slope of body fat against body fat free mass were 2.88 (s.e.m. = 0.09) in women and 1.84 (s.e.m. = 0.07) in men. There were no significant differences between the different body height classes. CONCLUSIONS: See results.

Adipose Tissue↗

Weibel-Palade bodies as a storage site of calcitonin gene-related peptide and endothelin-1 in blood vessels of the rat carotid body.

BACKGROUND: The vasculature of the carotid body has been considered to play a role in the regulation of blood flow into this organ. This light and electron microscope immunocytochemistry deals with endothelium-dependent vasomotion by vasodilatory calcitonin gene-related peptide (CGRP) and vasoconstrictive endothelin-1 (ET-1). METHODS: After adult male rats were perfused with a solution of periodate-lysine-paraformaldehyde through the left ventricle, the carotid artery bifurcations were isolated and utilized for light and electron microscope immunolabelings with CGRP and ET-1 primary antisera. RESULTS: By light microscope immunocytochemistry, immunoreactions to CGRP were seen along the endothelium of the carotid body artery (CBA) and its branches, and those of ET-1 were observed along the endothelium of the intralobular capillaries in addition to the above vessels. By immunoelectron microscopy, immunoreactive gold particles of CGRP and ET-1 were identified in the rough endoplasmic reticulum (rER) and in the Weibel-Palade (WP) bodies of endothelial cells of the CBA and its branches. Colocalization of both immunoreactive gold particles was observed in the same WP body. Immunoreactive gold particles of CGRP were also identified in the rER, Golgi apparatus, and specific granules of the dark glomus cells. CONCLUSIONS: Conceivably, CGRP and ET-1 are synthesized in the rER of these endothelial cells and are stored in the WP bodies for the autoregulation of blood flow.

Animals↗

The impact of body image experiences: development of the body image quality of life inventory.

OBJECTIVE: Substantial research confirms that body image affects multiple aspects of psychosocial functioning, yet there is no direct assessment to quantify its impact on an individual's quality of life. METHOD: In the present study, the Body Image Quality of Life Inventory was developed and empirically evaluated with 116 college women. RESULTS AND DISCUSSION: This 19-item assessment was internally consistent and stable over a 2- to 3 week period. It converged significantly with multiple measures of body image evaluation and investment and with body mass. The results further revealed that women in the sample reported more positive than negative consequences of their body image for various domains of life. The implications of these findings and directions for continued validation of this unique measure are considered.

Adult↗

Effect of regain of body weight on regional body fat distribution: comparison between pre- and postmenopausal obese women.

The aim of our study was to determine if regain of body weight increases visceral fat in obese women and if regain of weight has a different effect upon pre- and postmenopausal women. Twenty obese women (11 pre- and 9 postmenopausal) underwent a very low energy diet (VLED) for 2 weeks to lose weight. They then regained body weight in spite of the recommended hypocalorie diet. No significant modifications in body fat distribution indexes were found by computed tomography between VLED and after regain of weight. No significant changes were found in metabolic variables. No interactions between menopausal status and regain of body weight were observed. In conclusion, regain of weight does not seem to cause an increase in visceral fat; both pre- and postmenopausal women showed the same body fat distribution before weight loss and after regain of weight.

Adipose Tissue↗

Effect of heptanol on the short circuit currents of cornea and ciliary body demonstrates rate limiting role of heterocellular gap junctions in active ciliary body transport.

Rabbit ciliary body and cornea were mounted in Ussing-type chambers in Tyrode's under voltage clamp and the effects of heptanol, a gap junction inhibitor, on the short circuit current generated by each of the respective epithelia were determined. Studies were carried out either in control conditions or following amphotericin B permeabilization of either the basolateral membrane of the nonpigmented epithelium of the ciliary body or the apical membrane of the corneal epithelium, respectively. Previous studies have shown that, following these permeabilizations, short circuit currents are established, reflecting aqueous (or tear)-to-serosa Na+ fluxes, and that Na+ translocation through gap junctions connecting the individual layers of these tissues constitutes the major rate limiting step. Heptanol inhibited most of the short circuit current of the amphotericin B-modified ciliary body and cornea and of the unmodified ciliary body epithelium (control). In all these cases, the apparent IC50 was about 0.8 M. In the unmodified corneal epithelium, where ion translocation across the apical membrane constitutes the main rate limiting step for active secretion, 0.4 or 0.8 mM heptanol induced short circuit current increases; partial inhibition was observed only at high concentrations known to cause maximal inhibition of junctional permeability. Heptanol also enhanced the volume regulatory decrease of cultured human NPE cells, a process dependent on cell swelling-induced stimulation of Cl- and K+ permeabilities. Combined with our previous results demonstrating the lack of heptanol effects on other epithelial functions, these data suggest that the effect of heptanol on the active ciliary body transepithelial transport is primarily due to inhibition of the nonpigmented-pigmented junctional path and that this path is a potential site of rate limitation for the secretory process.

Alcohols↗

Body image and comprehension of body part names.

The relation between awareness of body topology and auditory comprehension of body part names was studied in 22 aphasic subjects. Two nonverbal tasks--human figure drawing and placement of individual body parts in relation to a drawn face--were compared with two auditory tests of body part comprehension. The two nonverbal and the two verbal tasks were closely correlated with each other, but there was no relation involving either of the verbal tests with either of the nonverbal tests. Selection errors in the auditory comprehension tasks were predominantly semantically based and equally distributed between functionally analogous parts and parts related by location on the body.

Aged↗

Whole body and regional retention of Tc-99m-labeled diphosphonates with a whole-body counter: a study with normal males.

A collimated whole-body counter was used to measure the retention and distribution of radioactivity along the longitudinal axis of the body at several times during the 24 hours after the intravenous injection of 50 microCi of Tc-99m-diphosphonates. Whole-body retention (WBR) was measured together with regional uptakes in the following four areas: head, chest, bladder, and legs using two structurally related Tc-99m-diphosphonate skeletal imaging agents: 1-hydroxyethylidene diphosphonate (HEDP) and methylene diphosphonate (MDP). The average 24 hour WBR values in young males, reflecting skeletal uptake of these tracers, were 17.7 +/- 2.2% (n = 20) and 31.0 +/- 2.4% (n = 3), respectively. A model of skeletal clearance was developed using the sum of two exponentials. In normal volunteers the initial rapid clearance phase of both tracers had a half-time of about 1 hour, whereas the slower second phase clearance had a half-time of 22 hours with HEDP and 44 hours with MDP. The WBR is usually calculated for the entire body only at 24 hours, but with the improved spatial resolution of a collimated whole-body counter, regional measurements could potentially be done over shorter periods (6-8 hours) in order to simplify the procedure.

Bone Diseases↗

Effects of a 3-week integrated body weight reduction program on leptin levels and body composition in severe obese subjects.

The effects of short-term (3 weeks) integrated body weight reduction (BWR) program (including energy-restricted diet, aerobic and strength exercise (5 days/week), nutritional education and psychological counseling) on plasma leptin levels and body composition were investigated in 54 morbidly obese patients (38 females/16 males; mean BMI +/- SE: 41.8 +/- 0.1 kg/m2, range 35-58 kg/m2; mean age: 29.8 +/- 1.0 yr, age range: 18-46 yr). The BWR program induced a significant (p < 0.001) weight loss (BMI reduction: -4.8%) and a significant modification in body composition, consisting in a fat mass (FM) decrease (-7.0 +/- 0.4%, p < 0.001) with a concomitant fat-free (FFM) mass increase (1.8 +/- 0.3%, p < 0.001). On average, plasma leptin levels decreased significantly both in males (from 19.4 +/- 2.6 ng/ml to 11.6 +/- 1.3 ng/ml, p < 0.001) and in females (from 41.1 +/- 3.6 ng/ml to 29.9 +/- 3.0 ng/ml, p < 0.001). Both before and after weight loss, leptin levels were positively correlated (p < 0.001) with BMI and percent fat mass (FM) values. Weight changes after the BWR program were negatively correlated with baseline leptin concentrations both in absolute terms and expressed per unit FM. In conclusion, a short-term diet plus aerobic/strength training effectively induces body composition changes and reduces plasma leptin levels. Body FM reduction appears to be not the unique determinant of leptin levels regulation and the degree of leptin over-expression may negatively affect weight loss in morbidly obese patients.

Adipose Tissue↗

Effect of a 3-week body mass reduction program on body composition, muscle function and motor performance in pubertal obese boys and girls.

The aim of this study was to investigate the effects of a multidisciplinary body mass reduction (BMR) program on body composition, muscle function and motor performance in 50 obese [mean body mass index (BMI): 35.9+/-5.8 kg/m2] boys and girls aged 12-17 yr (Tanner stage III, IV and V). The hospital-based BMR program combined an energy-restricted diet (1400-1600 kcal), nutritional education, psychological counselling and moderate physical activity (45-60 min/ session; 5 sessions/week; 60-80% of the maximal heart rate) during a 3-week period. Fat mass, fat-free mass, maximal power during jumping and stair climbing as well as maximal strength of the upper and lower limb muscles were quantified before and after the treatment. Body mass and fat mass significantly decreased following the BMR program, respectively -5.1 and -7.8% (p<0.001), while percent fat-free mass increased 2.3% (p<0.001). The treatment significantly increased both stair climbing and jumping power, respectively 8.2 and 8.9% (p<0.05), and the same was true for maximal strength of the upper and lower limb muscles (p<0.001). For the first time, it was demonstrated that a BMR program entailing diet and physical exercise significantly improved body composition, muscle function and motor performance in obese boys and girls aged 12-17 yr, while gender and pubertal stages had no influence on BMR program-induced changes.

Adolescent↗

Healthy bodies, social bodies: men's and women's concepts and practices of health in everyday life.

Using interview data from white, middle-class men and women, ages 35-55, the research explores the phenomenological, embodied aspects of health. Health is found to be grounded in a sense of self and a sense of body, both of which are tied to conceptions of past and future actions. Gender is a leitmotif. The body, as the focal point of self-construction as well as health construction, implicates gender in the everyday experience of health. The interplay between health, self, body, and gender at the individual level is linked to the creation of a sense of healthiness in the body politic of society. If social psychological theories of health are to reflect adequately the everyday experience of health, they must begin to take into account the body as individually and socially problematic.

Attitude to Health↗

The presence of tau distinguishes Lewy bodies of diffuse Lewy body disease from those of idiopathic Parkinson disease.

The antigenic components of Lewy bodies in the cerebral cortex and substantia nigra in 5 cases of diffuse Lewy body disease were examined by immunocytochemistry, using antibodies to neurofilaments (in the phosphorylated or non-phosphorylated forms); to ubiquitin; to the microtubule-associated proteins MAP1, MAP2 and tau; to isolated Alzheimer paired helical filaments, and to tubulin, in the tyrosinated and non-tyrosinated forms. Immunoreactivity with antibodies to cytoskeletal components was identical to that previously described for Lewy bodies of idiopathic Parkinson disease, with the exception that the inclusions of diffuse Lewy body disease (in both cortex and substantia nigra) were stained by an antibody to tau protein. Our findings indicate that although the inclusions found in diffuse Lewy body disease share structural and epitopic features with the inclusions of idiopathic Parkinson disease, they also have distinguishing characteristics (in addition to the differing neuronal populations involved). Also, they suggest that although the inclusions in both conditions appear similar, they probably have different pathogenetic origins.

Aged↗

Effects of dietary phytase on body weight gain, body composition and bone strength in growing rats fed a low-zinc diet.

Phytic acid, a major phosphorous storage compound found in foodstuffs, is known to form insoluble complexes with nutritionally essential minerals, including zinc (Zn). Phytases are enzymes that catalyze the removal of these minerals from phytic acid, improving their bioavailability. The objective of the present study was to determine the ability of dietary phytase to affect body weight, body composition, and bone strength in growing rats fed a high phytic acid, low Zn diet. Rats (n = 20) were fed either a control (AIN-93) or phytase supplemented (Natuphos, BASF, 1,500 phytase units (FTU)/kg) diet for a period of 8 weeks. Phytase supplementation resulted in increased (P<.05) bone and plasma Zn, but no change in plasma inorganic phosphorous or bone levels of Ca, Fe, or Mg. The addition of phytase to the diets resulted in a 22.4% increase (P<.05) in body weight at the end of the study as compared with rats fed a control diet. Dual x-ray absorptiometry (DXA) revealed that phytase supplementation resulted in increase lean body mass (LBM, P<.001) and increased bone mineral content (BMC, P<.001) as compared with feeding the control diet. Bone studies indicated that femurs and tibias from phytase supplemented rats had greater mass (P<.05) and were stronger (P<.05) than rats fed the control diet. This data suggest that the addition of phytase to low Zn diets results in improved Zn status, which may be responsible for beneficial effects on growth, body composition, and bone strength.

6-Phytase↗

Prevalence of tattooing and body piercing in Germany and perception of health, mental disorders, and sensation seeking among tattooed and body-pierced individuals.

OBJECTIVE: The objective of this study was to obtain data on the incidence and relationship of psychological factors to tattooing and body piercing from a large and representative sample of German citizens (N=2043). METHODOLOGY: Representative data (sample age range=14-93 years) were evaluated with respect to health-related quality of life (SF-36), mental health (General Health Questionnaire), mental disorders (Patient Health Questionnaire), and sensation seeking (Arnett Inventory of Sensation Seeking). RESULTS: The prevalence of tattooing and that of body piercing in the general German population are 8.5% and 6.5%, respectively. Individuals aged between 14 and 24 years display the highest rate of body piercings or tattoos (females, 41%; males, 27%). Within the group of individuals aged between 14 and 44 years, unemployment and nonaffiliation to a church are positively correlated, tattooing is significantly correlated with the perception of reduced mental health, and both tattooing and body piercing are correlated with significantly increased sensation-seeking behavior. CONCLUSIONS: Next to being motivated by fashion and the urge to fit in with one's peers, the major reasons for body modification practices in the German population appear to be negatively perceived conditions of life, reduced social integration, and increased sensation-seeking behavior.

Adolescent↗

Eyelid foreign body mimics an intraocular foreign body on plain orbital radiography.

Localization of a foreign body detected on plain orbital radiography may be achieved by comparing radiographs taken with the eyes in upgaze and downgaze. Movement of the foreign body with ocular rotation is considered to indicate localization either within the globe itself or within the soft tissues of the orbit closely related to the globe. A case is reported that demonstrates that this radiologic feature may also occur when a foreign body is located within an eyelid because the position of the eyelids also changes on vertical eye movements. An eyelid foreign body may therefore mimic an intraocular foreign body on plain orbital radiography.

Adult↗

Postnatal development of body mass and of hepatic xenobiotics biotransformation of male rats with low body mass at birth.

Body mass gain of male rats with low body mass at birth was lower from birth up to the 5th postnatal day, but higher from the days 5 to 30 in comparison with male litter mates with normal body mass at birth. Absolute body and liver masses were significantly lower up to the 30th postnatal day, but fully improved at adult age. No differences of ethoxycoumarin O-deethylation activities existed between both groups at all ages. Ethylmorphine N-demethylation activity was significantly lower in newborn males with low body mass, but had been fully developed as early as the 5th postnatal day. We conclude that postnatal adaptation of male rats with spontaneous growth retardation at birth is slightly delayed only during the first days of life.

Animals↗

The relationship between body-image and body-fat in adult women.

Measures of body-fat or adiposity, body-image, and other psychological variables were obtained from a sample of 100 female volunteer subjects. The measures of adiposity included surface anthropometry, underwater weighing and a new electrical impedance method. Body-image measures were derived from two experimental techniques, namely the visual-size estimation (or calliper) method and a distorting-mirror, and also from a self-report body-satisfaction scale (BSS). The main findings were that measures of adiposity were positively and significantly related to each other. But, that by contrast, measures of body-image separated out into three distinct sets, namely: (i) 'mirror' accuracy, (2) 'callipers' accuracy, and (3) 'dissatisfaction/desire for change'. The 'accuracy' measures were found to be unrelated to measures of adiposity or other psychological variables: while the 'dissatisfaction' measures were found to relate to both of these. The implications of these findings for future studies of weight and eating disorder groups are discussed.

Adult↗

Perceptual distortion of body image in adolescent girls: distortion of body image in adolescence.

Body image estimations in 86 normal-weight adolescent girls showed (a) a tendency to overestimate body width and underestimate body length and (b) an association between the younger ages and the extent of overestimation of body width. In particular there is a sharp drop in overestimation between ages 10 and 11 and thereafter a more gradual and non-significant decline. These age-specific normative data are useful in assessing body image distortions in a variety of medical and psychiatric disorders.

Adolescent↗