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Recent mass balance of polar ice sheets inferred from patterns of global sea-level change.

Global sea level is an indicator of climate change, as it is sensitive to both thermal expansion of the oceans and a reduction of land-based glaciers. Global sea-level rise has been estimated by correcting observations from tide gauges for glacial isostatic adjustment--the continuing sea-level response due to melting of Late Pleistocene ice--and by computing the global mean of these residual trends. In such analyses, spatial patterns of sea-level rise are assumed to be signals that will average out over geographically distributed tide-gauge data. But a long history of modelling studies has demonstrated that non-uniform--that is, non-eustatic--sea-level redistributions can be produced by variations in the volume of the polar ice sheets. Here we present numerical predictions of gravitationally consistent patterns of sea-level change following variations in either the Antarctic or Greenland ice sheets or the melting of a suite of small mountain glaciers. These predictions are characterized by geometrically distinct patterns that reconcile spatial variations in previously published sea-level records. Under the--albeit coarse--assumption of a globally uniform thermal expansion of the oceans, our approach suggests melting of the Greenland ice complex over the last century equivalent to -0.6 mm yr(-1) of sea-level rise.

Journal Article↗

River plumes as a source of large-amplitude internal waves in the coastal ocean.

Satellite images have long revealed the surface expression of large amplitude internal waves that propagate along density interfaces beneath the sea surface. Internal waves are typically the most energetic high-frequency events in the coastal ocean, displacing water parcels by up to 100 m and generating strong currents and turbulence that mix nutrients into near-surface waters for biological utilization. While internal waves are known to be generated by tidal currents over ocean-bottom topography, they have also been observed frequently in the absence of any apparent tide-topography interactions. Here we present repeated measurements of velocity, density and acoustic backscatter across the Columbia River plume front. These show how internal waves can be generated from a river plume that flows as a gravity current into the coastal ocean. We find that the convergence of horizontal velocities at the plume front causes frontal growth and subsequent displacement downward of near-surface waters. Individual freely propagating waves are released from the river plume front when the front's propagation speed decreases below the wave speed in the water ahead of it. This mechanism generates internal waves of similar amplitude and steepness as internal waves from tide-topography interactions observed elsewhere, and is therefore important to the understanding of coastal ocean mixing.

Journal Article↗

A model of hydraulic interactions in liver parenchyma as forces behind the intrahepatic bile flow.

The small diameters of bile canaliculi and interlobular bile ducts make it hard to attribute the bile flow solely to the process of secretion. In the model liver within its capsule is considered a limited space in which volume expansions of one part are possible only through the shrinking of other parts. The liver capsule allows only very slow volume changes. The rate of blood flow through the sinusoides is governed by the Poisseuill-Hagen law. The model is based on a concept of circulatory liver units. A unit would contain a group of acini sharing the same conditions of arterial flow. We can imagine them as an acinar group behind the last pressure reducer on one arterial branch. Acini from neighboring units compose liver lobules and drain through the same central venule. One lobule can contain acini from several neighboring circulatory units. The perfusion cycle in one unit begins with a transient tide in the arterial flow, governed by local mediators. Corresponding acini expand, grabbing the space by compressing their neighbors in the same lobules. Vascular resistance is reduced in dilated and increased in compressed acini. Portal blood flows through the dilated acini, bypassing the compressed neighbors. The cycle ends when the portal tide slowly diminishes and acinar volume is back on the interphase value until the new perfusion cycle is started in another circulatory unit. Each cycle probably takes minutes to complete. Increased pressures both in dilated and in compressed acini force the bile to move from acinar canalicules. Both up and down changes in acinar volume might force the acinar biliary flow. In cases of arterial vasoconstriction, increased activity of vasoactive substances would keep most of the circulatory units in the interphase and increased liver resistance can be expected. Liver fibrosis makes all acini to be of fixed volume and result in increased resistance. Because of that, low pressure portal flow would be more compromised, as reported. In livers without arterial blood flow, although some slow changes in the portal flows can be expected, acinar volume changes should be reduced. In acute liver injury, enlarged hepatocytes would diminish sinusoidal diameter and increase acinar resistance. In liver tumors, areas of neovascularization with reduced resistance would divert the arterial flow from the normal tissue, while in the compressed perifocal areas, increased vascular resistance should diminish mainly the portal flow.

Bile↗

Generation, propagation, and breaking of internal solitary waves.

Tidal, two-layer flow over topography generates a kink of the interface separating an upstream interfacial elevation from a depression above the topography. Upstream undular bores and solitary waves of large amplitude are generated from the interfacial kink. The waves propagate upstream when the tide turns. Interfacial simulations of this kind of generation process fit with the observations at Knight Inlet in British Columbia, in the Sulu Sea experiment, and undular bores generated by internal tides in the Strait of Gibraltar. Fully nonlinear interfacial computations compare successfully with experimental observations of solitary waves in the laboratory and in the field for wave amplitudes ranging from small to maximal values. The waves exhibit only minor sensitivity to a finite thickness of the pycnocline. Analytical solitary waves are recaptured in the small amplitude limit. Shear-induced breaking appears first in the top part of the pycnocline and is expressed in terms of the Richardson number. Convective breaking in the top part of the water column occurs beyond a threshold amplitude when a pronounced stratification continues all the way to the ocean surface.

Algorithms↗

Effect of acanthocephalan parasites on hiding behaviour in two species of shore crabs.

The effect of acanthocephalan parasites (Profilicollis spp.) on the hiding behaviour during low tide of two species of shore crabs (intermediate hosts), Macrophthalmus hirtipes (Brachyura: Ocypodidae) and Hemigrapsus crenulatus (Brachyura: Grapsidae), was examined at Blueskin Bay, South Island, New Zealand. Exposed M. hirtipes were found to have significantly higher infection levels than did hidden conspecifics. This pattern was not observed for H. crenulatus. Mean cystacanth numbers were found to be considerably higher in M. hirtipes than H. crenulatus. Crabs exposed at low tide are at a greater risk of predation by definitive shorebird hosts than are hidden conspecifics. Preferential manipulation of one intermediate host species over another could influence diversity within ecosystems.

Acanthocephala↗

Field studies on ozone inactivation of a Gymnodinium breve toxin.

Water samples were collected from Boca Ciega Bay (St. Petersburg) during the April, 1974, red tide that occurred on the Florida west coast. The causative agent of this phenomenon was the toxic dinoflagellate, Gymnodinium breve. The toxic red tide samples were treated with ozone gas and rendered nonlethal, as measured by mouse injection.

Animals↗

How we changed from paper to online education: Teaching Immunization Delivery and Evaluation.

This article discusses meeting the challenges encountered in changing a paper-based, interactive immunization delivery curriculum into an online, self-paced format. The program, TIDE (Teaching Immunization Delivery and Evaluation), was developed through collaborative efforts of medical educators and content experts with initial funding from the Centers for Disease Control and Prevention, the Ambulatory Pediatric Association and the Medical University of South Carolina. We summarize the efforts of the development team to create interactive case scenarios, provide ready access to resource materials and an audit tool for assessing the immunization rate of the learner's clinic or practice, and meet the rigorous requirements of awarding continuing education credit. Data based on more than 100 doctors' and nurses' evaluations indicate a trend toward higher overall ratings of the online version than the paper version (88% online vs. 76% paper reported module as very good or excellent). As the TIDE program is evaluated, the team's goal is to incorporate instructional methods to increase relevance and learners' opportunities for 'learning by doing.' Future plans include extending online office assessment tools to encourage use for continuous quality improvement, and providing a mechanism for learners to share their techniques for obtaining and maintaining high immunization rates.

Computer-Assisted Instruction↗

Brevetoxin-induced neural insult in the retrosplenial cortex of mouse brain.

Brevetoxins (polyether breve toxins; PbTx) are polyether neurotoxins produced by the marine dinoflagellate Karenia brevis, an organism associated with red tide blooms in the Gulf of Mexico and along the Atlantic coast from Florida to North Carolina. Brevetoxin-3 (PbTx-3) is a major component of the array of brevetoxins found in marine aerosols measured along red tide affected beaches. Humans exposed to aerosolized brevetoxins for short periods of time often suffer a variety of adverse health effects. It was consequently of interest to assess the potential for aerosolized brevetoxin to produce a neurotoxic response. Female BALB/c mice were exposed nose-only for 2 consecutive days to PbTx-3 aerosol, with a 2-h exposure on the first day and a 4-h exposure on the second day. The average PbTx-3 exposure concentrations on days 1 and 2 were 312 +/- 113 mug brevetoxin 3/m3 and 278 +/- 24 mug brevetoxin 3/m3, respectively. The brevetoxin-containing aerosol had a mass median aerodynamic diameter of 0.92 mum with a geometric standard deviation of 1.38. Coronal sections of mouse brains were evaluated for neuronal damage using both silver and Fluoro-Jade B staining to identify degenerating neuronal elements. PbTx-3 inhalation exposure produced neuronal degeneration in the posterior cingulate/retrosplenial cortex of mice as evidenced by silver-positive degenerating neurons in this region. No staining was found in other regions of the PBTx-3-exposed mouse brains or in brains of control, sham-exposed mice. The existence of a neurotoxic insult in PbTx-3-exposed mice was confirmed using Fluoro-Jade B to label degenerating neurons. Fluro-Jade-positive neurons were observed in the retrosplenial cortex of PBTx-3 exposed, but not control, mice. These results suggest that subacute exposure to PbTx-3 for 2 days is sufficient to induce neuronal degeneration in a discrete region of the mouse cerebral cortex.

Animals↗

Uptake, tissue distribution, and excretion of brevetoxin-3 administered to mice by intratracheal instillation.

Brevetoxins are a family of potent lipid-soluble neurotoxins produced by the dinoflagellate Karenia brevis, the organism responsible for Florida red tide. Brevetoxins aerosolized by surf and wind produce irritation of the eyes, nose, and throat in people on or near red tide-affected beaches. The effects of chronic exposures to brevetoxins on healthy and health-compromised individuals are not known. The purpose of this study was to investigate the pulmonary uptake, tissue distribution, and excretion of polyether brevetoxin-3 in mice, a rodent model for investigating the potential systemic adverse health effects associated with repeated brevetoxin inhalation. Male CBA/CaJ mice were administered [3H]brevetoxin-3 by intratracheal instillation. Groups of 3 mice were sacrificed immediately after instillation and at 0.5, 3, 6, 12, 24, 48, and 96 h postinstillation. Four additional mice were placed into metabolism cages for excreta collection up to 168 h postinstillation. Brevetoxin-3 distributed rapidly to all tissues, with the highest initial doses in the liver and gastrointestinal tract. Elimination half-times ranged from approximately 28 h for fat, heart, intestines, kidneys, liver, and muscle to approximately 90 h for brain and testes. The total dose to tissue ranged from 39 ng brevetoxin equivalents-h/g for testes to 406 ng brevetoxin equivalents-h/g for liver. Approximately 90% of excretion had occurred within 96 h, with 11 and 64% of the initial brevetoxin dose excreted in urine and feces, respectively. These results are consistent with earlier reports of rapid absorption and widespread tissue distribution of brevetoxins in rats.

Administration, Inhalation↗

Time-keeping system of the eel pout, Zoarces viviparus.

Observations on the rhythmic activity of 71 juvenile specimens of the inter-tidal blenny Zoarces viviparus reveal an endogenous pattern of swimming at three different periodicities. Circatidal swimming, with activity peaks phased to high water or the ebb of the subjective 12.4-h tides, was found in 50 fish and was the predominant pattern seen immediately after collection, when the rhythm generally persisted for between 3 and 12 cycles. Discrete activity peaks, with a free running period of approximately 24 h were also evident in the swimming pattern of eight fish. A circadian influence was also manifest as a modulation in amplitude, phase shifts and changes in free-running period of the circa-tidal rhythm. Overall, the activity level declined with time but those fish that remained active long enough showed a semi-lunar rhythm, with maximum activity at the time of the spring tides. A comparison of the behavior of animals collected at different times of the year suggests a seasonal variation in the persistence of circatidal swimming. The results are consistent with a control system involving circatidal, circadian, and semi-lunar oscillators.

Activity Cycles↗

An anterior-posterior gradient of refractive index in the ameba and its significance in ameboid movement.

Sustained locomotion in Amoeba proteus and Amoeba dubia results in the establishment of a measureable gradient of refractive index along the anterior-posterior axis of the cell, provided thickness of the specimens is kept constant by even compression under a selected coverglass supported by quartz beams of uniform diameter. The tail region of the ameba develops a higher refractive index, indicative of from 6 to 40 per cent more organic matter (expressed as protein) there than present in the front. This gradient fades on cessation of movement. The average protein concentrations in the crystal-free tails and fronts of 15 A. proteus were 3.9 and 3.4 per cent, respectively. In individual experiments, the tail-front difference ranged from one to eight times the accuracy of the method. Since the gradient of refractive index was shown not to result from extraction of water from the tail by the contractile vacuole, it was interpreted as displacement of water toward the anterior part of the cell during movement. It is suggested that contraction of the ectoplasm drives forward a "tide" of syncretic fluid, the anterior border of which is visible as the hyaline cap, which contains less than 1 per cent protein. The movement of the granular endoplasm into the hyaline cap would then complete the cycle by imbibition of the fluid tide. The theoretical positions of Pantin and of Dellinger have been combined in the proposal that ameba cytoplasm consists of a network of a contractile phase which is able to expel (by syneresis) a highly mobile fluid phase. Some other possible interpretations are discussed.

Amoeba↗

Cardiorespiratory effects of forced activity and digestion in toads.

Digestion and physical activity are associated with large and sometimes opposite changes in several physiological parameters. Gastric acid secretion during digestion causes increased levels of plasma bicarbonate ([HCO-3](pl)), whereas activity leads to a metabolic acidosis with increased lactate and decrease in plasma bicarbonate. Here we describe the combined effects of feeding and activity in the toad Bufo marinus to investigate whether the increased bicarbonate buffering capacity during digestion (the so-called alkaline tide) protects the acid-base disturbance during activity and enhances the subsequent recovery. In addition, we describe the changes in arterial oxygen levels and plasma ion composition, as well as rates of gas exchange, heart rates, and blood pressures. Toads were equipped with catheters in the femoral artery and divided into four experimental regimes: control, digestion, forced activity, and forced activity during the postprandial period (N=6 in each). Digestion induced a significant metabolic alkalosis with increased [HCO-3](pl) that was completely balanced by a respiratory acidosis; that is, increased arterial Pco(2) (P(a)co(2)), so that arterial pH (pH(a)) did not change. Forced activity led to a substantial reduction in pH(a) by 0.43 units, an increase in plasma lactate concentration by 12.5 mmol L(-1), and a reduction in [HCO-3](pl) of similar magnitude. While digesting animals had higher P(a)co(2) and [HCO-3](pl) at rest, the magnitude and duration of the changes in arterial acid-base parameters were similar to those of fasting animals, although the reduction in pH(a) was somewhat lower (0.32 units). In conclusion, while recovery from the acidosis following exercise did not seem to be affected by digestion, the alkaline tide did slightly dampen the reduction in pH(a) during activity.

Acid-Base Equilibrium↗

Metabolism during delayed hatching in terrestrial eggs of a marine fish, the grunion Leuresthes tenuis.

California grunion, Leuresthes tenuis (Osteichthyes: Atherinidae), leave the ocean to spawn in the sand following extreme high tides in summer. Eggs develop out of water and are able to hatch within 9-13 d, the primary hatching period. Eggs hatch on immersion and agitation by waves at the next extreme high-tide series. However, if the waves do not reach them and hatching is not induced, eggs remain viable in the sand for several weeks. During this period, grunion eggs will delay hatching, but will hatch at any time if immersed and agitated in seawater. Egg metabolism during embryonic development was measured by aerial respirometry. Metabolism increased linearly until the first date at which eggs were able to hatch. At that time, metabolic rate stabilized and remained constant for two additional weeks of embryonic incubation. Larvae induced to hatch after different durations of incubation within this period were not significantly different in length; however, the amount of oil contained in the yolk decreased linearly during the delayed hatching period. We suggest that the increase in grunion egg metabolism during the time to primary hatching capability, and the steady, high metabolic rate throughout the delayed hatching period, permit reproduction in the unique niche of these marine eggs. These semiterrestrial anamniotic eggs develop quickly and remain continuously ready to hatch over an extended period in response to an environmentally dependent and somewhat unpredictable embryonic timetable.

Adaptation, Physiological↗

Selective blockage of the 3'-->5' exonuclease activity of WRN protein by certain oxidative modifications and bulky lesions in DNA.

Individuals with mutations in the WRN gene suffer from Werner syndrome, a disease with early onset of many characteristics of normal aging. The WRN protein (WRNp) functions in DNA metabolism, as the purified polypeptide has both 3'-->5' helicase and 3'-->5' exonuclease activities. In this study, we have further characterized WRNp exonuclease activity by examining its ability to degrade double-stranded DNA substrates containing abnormal and damaged nucleo-tides. In addition, we directly compared the 3'-->5' WRNp exonuclease activity with that of exo-nuclease III and the Klenow fragment of DNA polymerase I. Our results indicate that the presence of certain abnormal bases (such as uracil and hypoxanthine) does not inhibit the exonuclease activity of WRNp, exo-nuclease III or Klenow, whereas other DNA modifications, including apurinic sites, 8-oxoguanine, 8-oxoadenine and cholesterol adducts, inhibit or block WRNp. The ability of damaged nucleo-tides to inhibit exonucleolytic digestion differs significantly between WRNp, exonuclease III and Klenow, indicating that each exonuclease has a distinct mechanism of action. In addition, normal and modified DNA substrates are degraded similarly by full-length WRNp and an N-terminal fragment of WRNp, indicating that the specificity for this activity lies mostly within this region. The biochemical and physiological significance of these results is discussed.

Adenine↗

Semilunar follicular cycle of an intertidal fish: the Fundulus model.

In their habitat, Fundulus heteroclitus (Cyprinodontidae) spawn against a variety of specific substrates that become available only at spring tides; they thus exhibit a semilunar reproductive cyclicity. In the laboratory at 28 +/- 0.6 degrees C and 14L:10D, this cyclicity is free-running and can be observed by means of daily egg collection and frequent ovarian sampling. Daily egg collection cycles gathered from three different spawning groups were pooled chronologically; the cycles were determined by nonlinear regression sine-curve matching and so were dated from Days -6 to +6, with Day 0 as the peak egg collection day. Ovaries were sampled on Days -6, -4, -2, -1, +1, +2, and +4. The pooled data indicated that early-maturing follicles (1.3- to 1.6-mm diameter) are recruited from a constant reserve of vitellogenic follicles (0.7- to 1.2-mm diameter) and become abundant early in the cycle but are depleted during Days -6 to +4 by the formation of late-maturing follicles and ovulated eggs. A midcycle peak of ovulated eggs in the ovary corresponds to the egg collection peak. This progression of follicles followed by spawning decreases the total count of the follicles (> or = 0.7-mm diameter) and eggs in the ovary by 52% (from 454 to 219 per 10 g of female weight). The semilunar follicular cycle in this Fundulus model thus includes 1) an early-cycle follicle recruitment, 2) an early- and midcycle follicle maturation, and 3) a midcycle egg ovulation and spawning. This follicular cycle also proceeds in fish deprived of a spawning substrate. A female in the wild would thus execute the follicular cycling in a timely sequence, in anticipation of each recurring spring tide, whether or not a natural substrate becomes available. We conclude that F. heteroclitus provides a useful nonmammalian model for the study of cyclic reproductive activity in the laboratory.

Animals↗

The origins of the concept of interference.

The concept of interference is implicit in Newton's explanation of the anomaly of the tides in the Gulf of Tongkin, but Thomas Young was the first to generalize the principle and to apply it to compound tides, to auditory beats, and to the colours of thin films. In his Bakerian Lecture, delivered on 12 and 19 November 1801, he was able to accurately derive the wavelengths of particular hues from Newton's measurements of the colours of thin plates. The first printed statement of the generalized principle of interference appears in the Syllabus for his Royal Institution lectures, which was published early in 1802. His celebrated two-slit experiment is first described in his Course of Lectures on Natural Philosophy and the Mechanical Arts of 1807.

England↗

What happened in 1953? The Big Flood in the Netherlands in retrospect.

During the weekend of Saturday 31 January to Sunday 1 February 1953, a storm tide raged across the northwest European shelf and flooded the low-lying coastal areas of the countries around the North Sea. The peak high waters occurred during the night and the storm surprised many people in their sleep. The resulting disaster in terms of loss of life and damage to infrastructure was enormous. In the Netherlands, 1836 people fell victim to the flood; in the UK and Belgium, the casualties were 307 and 22, respectively. The large number of fatalities in the Netherlands was related to the fact that much of the affected area is below sea-level.This paper focuses on the case of the Netherlands. It discusses the history of land reclamation, and the fact that living in low-lying areas protected by dykes, often below sea-level, is an accepted fact of life in the Netherlands. The historical approach to dyke maintenance is then outlined, and the state of the dykes in the early twentieth century and after the war is discussed. The characteristics of the storm and the flood are discussed, along with people's experiences of the first hours and days following the flood. The impact of this human stress has often been lasting--many survivors continue to live with daily memories of the flood. Attention is given to the large-scale rescue and relief efforts, the closure of the dykes during the following nine months and the concept of the Delta Plan, designed to prevent such a large-scale disaster ever happening again. Although the 1953 storm was indeed a low probability event leading to very high storm-induced water-levels, and occurred in combination with spring tide, several arguments are presented that explain why this flood turned into a disaster of such a large scale. Equally, the question is raised whether the disaster could have been prevented. The paper concludes by noting the importance of awareness and preparedness in order to prevent a future storm threat of this scale turning into a disaster of the scope of the Big Flood of 1953.

Computer Simulation↗

Pumping test in a confined aquifer under tidal influence.

In a coastal environment, tide-induced head fluctuations can complicate the interpretation of drawdown data from pumping tests. For confined aquifers and sinusoidal tides, the superposition principle can be used to obtain a closed-form solution. After subtracting the net tidal effects, the drawdown data become amenable to the standard analyses. Numerical simulations have shown that the method is reliable when the distance of the monitoring well to the well does not exceed 10% of the distance between the well and the tidal boundary.

Models, Theoretical↗