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Studies on the refrigerated storage of wheat (Triticum aestivum). 2. Ergosterol, xanthomegnin, viomellein and brevianamide A after inoculation with Penicillium viridicatum.

Wheat seed was adjusted to 18, 20, 22, 24 and 26% moisture content (m.c.), and stored for 240 days at 4 or 10 degrees C following inoculation with a strain of Penicillium viridicatum producing the toxins, xanthomegnin (XA), viomellein (VIO), and brevianamide A (BA). Wheat kernels were not sterilized before inoculation. The concentration of ergosterol (ERG), a chemical indicator of fungal biomass, remained constant at 18% m.c./4 degrees C, but increased under the other conditions. The time before a detectable increase of ERG concentration was higher and the rate of ERG production lower with decreasing moisture content and temperature. XA and BA were produced at both temperatures at 20-26% m.c., VIO was produced at 22-26% m.c./4 degrees C and 20-26% m.c./10 degrees C. The results suggest or indicate that the onset of XA, VIO and BA production (detection limits: 10, 15, and 0.1 micrograms/kg, respectively) coincided with the onset of ERG production. Maximum toxin contents were lower with decreasing moisture content at both temperatures, but were similar at 4 and 10 degrees C at 22-26% m.c. It is concluded that wheat contaminated with P. viridicatum should not be stored beyond the onset of ergosterol production; maximum storage periods are recommended.

Alkaloids↗

[Changes in the lipid component of the muscle tissue of several species of fish during refrigeration and heat treatment].

By using univariate thin-layer chromatography on silicagel IC254 of the company "Chamapod Széchoslovakia" the qualitative and quantitative composition of the muscular tissue lipids in new oceanic fish--seriolella and epigonus was determined. It was ascertained that the qualitative composition of lipids changed during cold storage but insignificantly, due to hydrolysis of triglycerides and oxidation of phospholipids. In the course of heat treatment there occurs disintegration of the lipid-proteinic complexes attended by an accretion of phospholipids. An increment of free fatty acids is caused by the hydrolytic decomposition of triglycerides.

Animals↗