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PubMed1991

Variation of geosmin content in Anabaena cells and its relation to nitrogen utilization.

The addition of the proper amount of ammonium to the culture medium containing nitrate as nitrogen source enhanced the growth rate of Anabaena viguieri. The amount of geosmin produced by these cells varied with the concentrations of ammonium added. A negative correlation between the amount of geosmin produced and of the growth rate of cells was revealed. This was also found in cells grown on various forms of nitrogen sources. Without supply of any nitrogen compound, this organism is capable of fixing gaseous nitrogen, and under these conditions the cells grew relatively slowly. However, they produced more geosmin (per unit protein mass) than cells grown in the presence of combined nitrogen. The isolation of heterocysts, in which nitrogen was fixed, showed that these cells produced higher amounts of geosmin than vegetative cells. The possible relation of nitrogen assimilation to the production of geosmin in the cells was discussed.

Anabaena↗
PubMed2006

Phylogenetic fragrance patterns in Nicotiana sections Alatae and Suaveolentes.

We analyzed floral volatiles from eight tobacco species (Nicotiana; Solanaceae) including newly discovered Brazilian taxa (Nicotiana mutabilis and "Rastroensis") in section Alatae. Eighty-four compounds were found, including mono- and sesquiterpenoids, nitrogenous compounds, benzenoid and aliphatic alcohols, aldehydes and esters. Floral scent from recent accessions of Nicotiana alata, Nicotiana bonariensis and Nicotiana langsdorffii differed from previously published data, suggesting intraspecific variation in scent composition at the level of biosynthetic class. Newly discovered taxa in Alatae, like their relatives, emit large amounts of 1,8-cineole and smaller amounts of monoterpenes on a nocturnal rhythm, constituting a chemical synapomorphy for this lineage. Fragrance data from three species of Nicotiana sect. Suaveolentes, the sister group of Alatae, (two Australian species: N. cavicola, N. ingulba; one African species: N. africana), were compared to previously reported data from their close relative, N. suaveolens. Like N. suaveolens, N. cavicola and N. ingulba emit fragrances dominated by benzenoids and phenylpropanoids, whereas the flowers of N. africana lacked a distinct floral scent and instead emitted only small amounts of an aliphatic methyl ester from foliage. Interestingly, this ester also is emitted from foliage of N. longiflora and N. plumbaginifolia (both in section Alatae s.l.), which share a common ancestor with N. africana. This result, combined with the synapomorphic pattern of 1,8 cineole emission in Alatae s.s., suggests that phylogenetic signal explains a major component of fragrance composition among tobacco species in sections Alatae and Suaveolentes. At the intraspecific level, interpopulational scent variation is widespread in sect. Alatae, and may reflect edaphic specialization, introgression, local pollinator shifts, genetic drift or artificial selection in cultivation. Further studies with genetically and geographically well-defined populations are needed to distinguish between these possibilities.

Cyclohexanols↗
PubMed2003

The European perspective on nitrogen emission and deposition.

Europe has been successful in reducing the emissions of several nitrogenous pollutants over recent decades. This is reflected in concentrations and deposition rates that have decreased for several components. Emissions of nitrogen containing gases are estimated to have decreased in Europe by 10%, 21% and 14% for N(2)O, NO(x) and NH(3), respectively, between 1990 and 1998. The main reductions are the result of a decrease in industrial and agricultural activities in the east of Europe. The reductions are a result of the economic situation, measures in the transport sector, industry, and the agricultural sector, with only a small part of the reduction due to specific measures designed to reduce emissions. The reduction is significant but far from the end goal for large areas in Europe, in relation to different environmental problems. The Gothenburg Protocol will lead to reductions of 50% and 12% in 2010 relative to 1990 for NO(x) and NH(3), respectively. The N(2)O emissions are expected to grow by 9% between 1998 and 2010. Further reductions are necessary to reach critical limits for ecosystem protection, air quality standards, and climate change. Emissions of nitrogen compounds result from an overload of reactive nitrogen that is produced by combustion processes, by synthesis of ammonia, or by import from other areas as concentrated animal feeds. Some improvements can be made in the efficiency of combustion processes and agricultural systems. However, measures to reduce emissions substantially need to focus on decreasing the production or import of reactive N. Reactive N ceilings for regions, based on critical limits for all N-related effects, can help to focus such measures. An integrated approach might have advantages over the pollutant-specific approach, to combat nitrogen pollution. This could provide the future direction for European policy to reduce the impacts of excess nitrogen.

Air Pollutants↗
PubMed2001

Nitrogen emission and deposition: the European perspective.

Europe has been successful in reducing the emissions of several nitrogenous pollutants over recent decades. This is reflected in concentrations and deposition rates that have decreased for several components. Emissions of nitrogen containing gases are estimated to have decreased in Europe by 10%, 21%, and 14% for NO, NOx, and NH3, respectively, between 1990 and 1998. The main reductions are the result of a decrease in industrial and agricultural activities in the east of Europe as a result of the economic situation, measures in the transport sector, industry and agricultural sector, with only a small part of the reduction due to specific measures designed to reduce emissions. The reduction is significant, but far from the end goal for large areas in Europe in relation to different environmental problems. The Gothenburg Protocol will lead to reductions of 50 and 12% in 2010 relative to 1990 for NOx and NH3, respectively. The N2O emissions are expected to grow between 1998 and 2010 by 9%. Further reductions are necessary to reach critical limits for ecosystem protection, air quality standards and climate change. Emissions of nitrogen compounds result from an overload of reactive nitrogen, which is produced by combustion processes, by synthesis of ammonia or by import from other areas as concentrated animal feeds. Although some improvements can be made by improving the efficiency of combustion processes and agricultural systems, measures to reduce emissions substantially need to be focused on decreasing the production or import of reactive N. Reactive N ceilings for regions based on critical limits for all N-related effects can help to focus such measures. An integrated approach might have advantages over the pollutant specific approach to combat nitrogen pollution. This could provide the future direction for European policy to reduce the impacts of excess nitrogen.

Air Pollutants↗
PubMed2006

Marine mollusks from Australia and New Zealand: chemical and ecological studies.

Marine mollusks contain structurally diverse terpenes, polyketides, polypropionates and nitrogenous metabolites that may confer an ecological advantage on the host organism. From a chemical perspective, the most studied Australian taxa include representatives of the nudibranchs and sea hares, which are characterised by terpenes acquired from their specialised diets of sponges and algae, respectively. In contrast, siphonariid limpets that are prevalent on temperate seashores carry out de novo biosynthesis of polypropionate metabolites. Nitrogenous compounds isolated from Australian marine mollusks include precursors to the first commercially significant marine bioproduct, Tyrian Purple, and metabolites that are characteristic of ingested cyanobacteria.

Animals↗
PubMed2001

Environmental scenarios for the future nitrogen policy in Flanders, Belgium.

The agricultural sector accounts for two thirds of nitrogen losses in Flanders, Belgium. Since 1991 both the government and the farmers have been taking measures to reduce the nitrogen surplus. Initially, the manure policy was aimed at distributing the manure surplus equally across Flanders. At the same time, the growth of livestock was stopped by a strict licensing policy, which required "command and control" measures. In recent years, the policy has switched to the use of individual target commitments by farmers. The Flemish manure policy will be tightened even more as a result of international pressures. An ex ante evaluation of possible policy options was carried out using three different scenarios spread out until 2010 (Business As Usual, Additional Measures, and Sustainable Development). To do this, a sector-economic, regionalized, environmental, comparative static, partial equilibrium, mathematical programming model of the Flemish agriculture was developed. The nitrogen emission into the agricultural soil was calculated by means of a regional soil balance. European targets can only be reached with manure processing, reduced fertilizer usage, and a strong reduction of intensive livestock breeding activities. The atmospheric deposition of nitrogen compounds will strongly decrease in 2010 if additional measures are taken. This will also result in a strong reduction of nitrous oxide emissions.

Agriculture↗
PubMed2004

Treatment of swine wastewater using MLE process and membrane bio-reactor.

The aim of this study was to develop the optimum integrated treatment system for slurry type swine wastewater through field testing. Although composting and liquid composting are the most desirable processes to treat swine wastewater, inadequate composting has been blamed as critical non-point source pollutants. In the area with limited crop land and grass land, the most feasible method to handle slurry type swine wastewater would be that the solids portion from the solids/liquid separation process is treated by composting and then the liquid portion is treated by a series of wastewater treatment processes, including physicochemical treatment system and biological nutrient removal systems such as the modified Ludzack Ettinger (MLE) process and MLE process coupled with a membrane, to satisfy the different effluent criteria. When using the appropriate solids/liquid separation system, the removal efficiency of SS, COD(Cr), and TKN was 92.4%, 73.6%, and 46.0%, respectively and the amount of bulking agent required for composting and organic loading rate for the following wastewater treatment system can be reduced by 94.8% and 84.7%, respectively. When treating the effluent from solids/liquid separation process by MLE process, the optimal volume fraction for denitrification was 20% of total reactor volume and the optimum ratio of F/M and F(N)/M were increased with increase of C/N ratio. Since the effluent quality of MLE process is not enough to discharge, the DAF process was operated with addition of FeCl3 and cationic polyelectrolyte. The effluent from the DAF process was treated in the MLE process coupled with a crossflow ultrafiltration membrane to satisfy more stringent effluent criteria. When external carbon source is added to keep 6.0 of C/N ratio, the efficiency of denitrification is best. The optimum linear velocity and transmembrane pressure for MBR process was 1.8 m/sec and 2.1 atm, respectively. By addition of external carbon source, nitrogen compounds, especially NOx-N, were considerably removed. And by addition of powdered activated carbon, the removal efficiency of COD(Cr) and COD(Mn) and the membrane flux were increased dramatically.

Animals↗
PubMed2004

Chemiluminescence detection systems for the analysis of explosives.

The objective of this paper is to provide a comprehensive review of explosive detection by chemiluminescence (CL) through a summary of the relevant literature in the last 5 years and a synopsis of current research topics and developments. The literature reviewed is specially addressed for the detection of a group of high explosives, containing nitrogen compounds. Most explosives compounds contain either nitro or nitrate groups which make possible their detection and quantification using detection systems based on chemiluminescent reactions. Practical considerations and experimental requirements are indicated, and the possibilities and limitations are evaluated.

Chromatography, Gas↗
PubMed1989

Bio-markers and the search for extinct life on Mars.

Geologic and climatologic studies suggest that conditions on early Mars were similar to early Earth. Because life on Earth is believed to have originated during this early period (3.5 billion years ago), the Martian environment could have also been conducive to the origin of life. To investigate this possibility we must first define the attributes of an early Martian biota. Then, specific geographic locations on Mars must be chosen where life may have occurred (i.e. areas which had long standing water), and within these distinct locations search for key signatures or bio-markers of a possible extinct Martian biota. Some of the key signatures or bio-markers indicative of past biological activity on Earth may be applicable to Mars including: reduced carbon and nitrogen compounds, CO3(2-), SO4(2-), NO3-, NO2- [correction of NO2(2)], Mg, Mn, Fe, and certain other metals, and the isotopic ratios of C, N and S. However, we must also be able to distinguish abiotic from biologic origins for these bio-markers. For example, abiotically fixed N2 would form deposits of NO3- and NO2-, whereas biological processes would have reduced these to ammonium containing compounds, N2O, or N2, which would then be released to the atmosphere. A fully equipped Mars Rover might be able to perform analyses to measure most of these biomarkers while on the Martian surface.

Biological Evolution↗
PubMed2003

Nutritional requirements of Pochonia chlamydospora and ARF18, fungal parasites of nematode eggs.

Twenty carbohydrates (C), 18 nitrogen compounds (N), and 9 vitamins were examined for their effects on the growth and conidiation of the nematode-egg-parasitic fungi Arkansas Fungus 18 (ARF18, isolate 908) and Pochonia chlamydospora var. chlamydospora in solid and liquid cultures. Glycogen was the best, and inulin, D-(+)-galactose, and soluble starch were good C sources for the growth of ARF18 in both solid and liquid cultures. ARF18 could not utilize alpha-cellulose in liquid; alpha-lactose and D-mannitol in solid; and D-(+)-xylose, L-(-)-sorbose, and D-(-)-ribose in both solid and liquid cultures. Casein was the best N source for ARF18 growth in both solid and liquid cultures and L-aspartic acid, DL-glutamic acid, peptone, and L-histidine were good in solid culture. ARF18 could not utilize L-cystine and L-tyrosine in solid culture, and L-cystine, DL-methionine, peptone, L-proline, and ammonium nitrate in liquid culture. Supplement of vitamins appeared to be unnecessary for ARF18 to grow. For P. chlamydospora var. chlamydospora growth, all test C sources, except L-(-)-sorbose, alpha-cellulose, citric acid, and D-(+)-glucose, were good in both solid and liquid cultures. Most N compounds were good for P. chlamydospora var. chlamydospora growth with casein and peptone the best. Vitamins had limited effect on P. chlamydospora var. chlamydospora growth. P. chlamydospora var. chlamydospora conidiation was well supported by D-(-)-ribose, D-(-)-fructose, melibiose, and D-(+)-galactose as C sources and by L-aspartic acid, DL-glutamic acid, and L-arginine as N sources. Excluding myo-inositol from the medium containing all other test vitamins increased P. chlamydospora var. chlamydospora conidiation, while excluding pyridoxine appeared to reduce its conidiation.

Animals↗
PubMed2001

Altered TNF-alpha, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system.

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-alpha (TNF-alpha) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 +/- 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-alpha (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group (P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture (P < 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system (P < 0.05). These studies demonstrate alterations in LPS-induced TNF-alpha production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.

Amino Acids↗
PubMed2006

Autohydrolysis of agricultural residues: study of reaction byproducts.

Samples of rice husks and corn cobs were subjected to hydrothermal treatments in aqueous media under conditions leading to maximal xylooligomer concentration, and the reaction liquors were extracted with dichloromethane (DCM) to assess the type and amount of reaction byproducts with potential application as food ingredients and cosmetics. The identified DCM-soluble compounds were classified in four categories (sugar-derived compounds, lignin-derived compounds, nitrogen-containing compounds and fatty acids). The experimental results were compared with literature data.

Carbohydrate Metabolism↗
PubMed2003

[Effect of thermal treatments on the chemical characteristics of mora crab meat (Homalaspis plana)].

Marine species muscles present non-proteins nitrogenated compounds, used as quality index. They are total volatile basis (NBVT), trimethylamine oxide (TMAO) and trimethylamine (TMA). pH is considered too as a quality index. The aim of this work was to evaluate these parameters in a fresh and canned marine product from the V region, corresponding to mora crab (Homalaspis plana). Fresh pincer meat from mora crab was extracted and kept in ice until theits analysis and thermal process of the canned product. A 3(2) statistical design was applied, considering two variables with 3 levels: 15, 30 y 45 minutes time levels: 80 degrees, 100 degrees y 121 degrees C temperature levels. Nine conditions of time-temperature were obtained. The thermal treatment caused an increase in pH and BVT. The TMA was increased since reduction of TMAO.

Amines↗
PubMed2001

Nitrate Regeneration Coupled to Degradation of Different Size Fractions of DON by the Picoplankton in the Elbe Estuary.

Bacterial conversion of high (HMW > 3,000 Da) and low (LMW < 3,000 Da) molecular weight DON (dissolved organic nitrogen) was studied along the freshwater section of the Elbe estuary during the summer of 1997. Indigenous populations of picoplankton were incubated in a flow-through chamber that allowed a constant exchange of sterile, filtered Elbe water as the culture medium for the microorganisms, which remained within the chamber. Nitrogen conversion was followed by changes in the concentrations of total and low molecular weight DON and dissolved inorganic nitrogen compounds, the uptake of O2, and bacterial growth. Along the Elbe estuary, total DON concentrations varied between 0.69 and 1.1 mg N L-1, of which between 64 and 79% was LMW-DON. Ammonium was a minor nutrient present in the Elbe at concentrations below 0.26 mg N L-1. During incubation in the laboratory between 27 and 64% of the LMW-DON was consumed at rates ranging from 24 to 51 µg N L-1 h-1. HMW-DON was used only when the degradable LMW-DON pool became exhausted and accounted for between 60 and 100% of the HMW-DON. This produced an increase in the DON consumption rate between 43 and 79 µg N L-1 h-1. Nitrification rates were independent of the external ammonium concentration until it decreased to below 1 µg N-NH4 L-1. Most of the N in the nitrification process originated as NH4 regenerated from DON. Between 75 and 100% of the LMW-DON and ammonium consumed was rapidly converted to nitrate. This amount decreased to between 65 and 85% when HMW-DON was consumed in addition to the LMW-DON. DON and ammonium consumption supported nitrification rates up to 71 µg N L-1 h-1. The amount of DOC (dissolved organic carbon) degraded was not equivalent to the C:N ratio of the total dissolved organic matter. Calculations based on oxygen consumption for respiration and ammonium regeneration revealed that the substrates used during the incubations contained C:N at ratios of about 3:1. These results suggest that the nitrogen-rich compounds had been removed from the dissolved organic matter and subsequently consumed by bacteria, while the carbon skeleton remained mostly unaffected by the degradation processes.

Journal Article↗
PubMed2025

Effects of aerosol aging on composition and light-absorbance of nitrogen-containing organic compounds: Evidences from ultra-high-resolution mass spectrometry analysis.

Nitrogen-containing organic compounds (NOCs) are key components of particulate matter (PM), but their compositional evolution and light-absorbing properties during atmospheric aging remain poorly understood. In this study, ultra-high-performance liquid chromatography coupled with Orbitrap mass spectrometry was used to semi-quantitatively analyze 59 PM1 samples collected in Shanghai. NOCs accounted for 31 % and 64 % of the detected species in negative (ESI-) and positive (ESI+) ionization modes, respectively. Atmospheric aging significantly reduced the molecular diversity of polar organics, with both the number and mass concentration percentages of CHON- compounds showing significant negative correlations with aging degree. Van Krevelen analysis demonstrated a decrease in the number of carboxylic-rich alicyclic molecules and their CHON- contributions during the aging process (from 37.7 % in fresh samples to 21.2 % in aged samples). CHN+ compounds, a major NOCs group in ESI+ mode, also decreased with aging. Correlation analyses involving the Bep/(Bep+Bap) ratio, relative humidity, and mass absorption efficiency at 365 nm revealed a decline in light absorption capacity with aging, suggesting aqueous-phase oxidation as a dominant aging mechanism. CHON- and CHN+ compounds were identified as the principal light-absorbing constituents in PM1. This work provides new insights into the aging-induced transformations of NOCs in urban PM1, and their changing role in light absorption, highlighting the need for further investigation of the aging mechanisms of NOCs.

Aerosols↗
PubMed1991

Arxula adeninivorans, a yeast assimilating many nitrogenous and aromatic compounds.

A detailed description of the yeast species Arxula adeninivorans (syn. Trichosporon adeninovorans) was given. The yeast assimilated all the sugars, polyalcohols and organic acids used in the conventional carbon compound assimilation test rapidly, except for L-rhamnose, inulin, lactose, lactate and methanol. As nitrogen sources served all conventionally used compounds except creatine and creatinine. Several nitrogenous compounds, e.g. amino acids, purine derivatives, served as sole source of carbon, nitrogen and energy. This was also true of many primary n-alkylamines and terminal diamines, but of nitrogen-less analogous compounds such as alcohols, dialcohols, carboxylic acids and dicarboxylic acids only intermediates of general metabolism were assimilated. For observing growth at the expense of potentially toxic compounds the slant culture method was developed. In this test lactate was readily assimilated but proved to be toxic at the same time. Several aliphatic organic acids and their esters supported growth in this test, as was the case with several benzene compounds such as hydroquinone, 3- and 4-hydroxybenzoic acid, protocatechuic, gentisic, gallic and ferulic acids, 4-hydroxyacetophenone and 1-phenyl-n-dodecane.

Carbon↗
PubMed2005

Advances in coupling a commercial total organic carbon analyser with an isotope ratio mass spectrometer to determine the isotopic signal of the total dissolved nitrogen pool.

A new method has been developed to analyse 15N of the total dissolved nitrogen (TDN) pool. The method operates on a commercial total organic carbon (TOC) analyser coupled to an elemental analyser/isotope ratio mass spectrometer (EA-IRMS). Nitrogen compounds are combusted to nitric oxide (NO) and nitrogen dioxide (NO2) by high-temperature catalytic oxidation (HTCO), after which the NOx gas is transferred to an EA-IRMS for isotopic nitrogen analysis. The system is described, including five modifications of the system in order to overcome analytical problems. First, flow paths were modified to run both systems on helium as carrier gas, while complete sample oxidation was maintained. Secondly, the catalyst structure was adapted to allow high injection volumes at the given backpressures delivered by the EA system. Thirdly, we installed a Permapure dehumidification system as the standard Peltier element did not satisfy dehumidification requirements. Finally, we prevented the inflow of atmospheric nitrogen into the system. In a final stage, we are planning to automate the coupled system in order to run a continuous batch of up to 60 samples. We have obtained satisfactory results on the accuracy and precision of 180+/-1 per thousand potassium nitrate samples (IAEA, USGS-32). Running a batch of five samples resulted in a mean isotopic value of 178.8 per thousand with a standard deviation of 2.8 per thousand. Some important issues could not yet be addressed here, and will have to be evaluated once the system is running on a continuous base. However, the results appear promising and this system has the potential to become a method for TD15N analysis. An appropriate TD15N analysis method might open new challenges in aquatic and terrestrial ecosystem nitrogen studies, including a more comprehensive study of the dissolved organic nitrogen pool.

Journal Article↗
PubMed1988

Ammonia production from amino acids and urea in the caecal contents of the chicken.

1. Ammonia production from urea and amino acids in the caecal contents of the chicken was evaluated using 15N-labeled nitrogenous compounds. 2. About 43% of each of urea nitrogen and glutamine amide nitrogen was converted to ammonia nitrogen, but only 25% of epsilon-nitrogen of the added arginine, a precursor of urea, was found in ammonia. 3. Amino nitrogen of the separately added glutamic acid and glycine to be converted to ammonia was 19-20% of their added amounts, whereas that of alpha-alanine was 11%. 4. It is concluded that dietary and urinary amino acids and urea which find their ways into the caeca are useful nitrogen sources for ammonia production by microflora in the caeca of the chicken.

Amino Acids↗