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Isokinetic elbow joint torques estimation from surface EMG and joint kinematic data: using an artificial neural network model.

Because the relations between electromyographic signal (EMG) and anisometric joint torque remain unpredictable, the aim of this study was to determine the relations between the EMG activity and the isokinetic elbow joint torque via an artificial neural network (ANN) model. This 3-layer feed-forward network was constructed using an error back-propagation algorithm with an adaptive learning rate. The experimental validation was achieved by rectified, low-pass filtered EMG signals from the representative muscles, joint angle and joint angular velocity and measured torque. Learning with a limited set of examples allowed accurate prediction of isokinetic joint torque from novel EMG activities, joint position, joint angular velocity. Sensitivity analysis of the hidden node numbers during the learning and testing phases demonstrated that the choice of numbers of hidden node was not critical except at extreme values of those parameters. Model predictions were well correlated with the experimental data (the mean root-mean-square-difference and correlation coefficient gamma in learning were 0.0290 and 0.998, respectively, and in three different speed testings were 0.1413 and 0.900, respectively). These results suggested that an ANN model can represent the relations between EMG and joint torque/moment in human isokinetic movements. The effect of different adjacent electrode sites was also evaluated and showed the location of electrodes was very important to produce errors in the ANN model.

Adult↗

The effects of fatigue on the resultant joint moment, agonist and antagonist electromyographic activity at different angles during dynamic knee extension efforts.

Examination of the effects of fatigue on antagonist function can provide information on the role of antagonists in limiting the resultant joint moment and stabilizing the knee. Therefore, the purpose of this study was to examine the moment, agonist and antagonist electromyographic (EMG) activity levels at different angular positions during an isokinetic muscular endurance knee extension test. Fifteen healthy males (age 22.6+/-1.9 yr) performed 34 maximal isokinetic concentric efforts of the knee extensors at 120 degrees s(-1). The EMG activity of vastus medialis and biceps femoris was recorded using surface electrodes. The motion ranged from 90 degrees to 0 degrees of knee flexion. The average moment and average EMG (AEMG) at 10-35 degrees, 36-55 degrees and 56-80 degrees angular position intervals were calculated for each repetition. Twenty eight efforts were further analysed. The moment of force demonstrated a decline of 70% at the end of the test. Two-way repeated measures analysis of variance tests indicated that this decline was significant (p < 0.05). No significant effects of angular position on fatigue moment characteristics were found. The agonist (vastus medialis) AEMG during the first repetition demonstrated a significant increase of 40-60% towards the middle part of the test (p < 0.05). In the second part of the test, the VM AEMG at longer muscle lengths was significantly higher compared to the initial efforts whereas the AEMG at short muscle lengths returned to initial values. The antagonist AEMG at all angular positions did not change significantly during the test. The decline in the resultant joint moment could be attributed to the effects of fatigue on the agonist muscle function. The agonist AEMG fatigue-patterns are dependent on the length of the muscle and may be due to alterations in the motor unit recruitment and/or activation failure in the quadriceps muscle. The biceps femoris maintains constant submaximal (21-33% of the maximum) AEMG activity which may play an important role in the stability of the knee joint. The contribution of antagonist activity to the resultant joint moment increases during the last part of an isokinetic concentric muscle endurance test.

Adult↗

Direct effects of triiodothyronine on human internal mammary artery and saphenous veins.

OBJECTIVE: Thyroid hormone (3,5,3'-triiodo-L-thyronine is under investigation as a positive inotrope and vasodilator for patients undergoing cardiac surgery. This study determined the direct effects of triiodothyronine on human blood vessels. DESIGN: Prospective, controlled, in vitro study. SETTING: Laboratory facility in a university teaching hospital. PARTICIPANTS: Small excess segments of internal mammary arteries or saphenous veins were obtained from patients undergoing coronary artery bypass surgery. INTERVENTIONS: Vessel segments were cut into rings to measure isometric tension development in isolated tissue baths containing Krebs-Ringer bicarbonate solution at 37 degrees C. Rings were prestretched in vitro to resting tensions analogous to mean arterial or central venous pressures in vivo and then constricted with potassium or phenylephrine. Rings were exposed to increasing concentrations of triiodothyronine (4 x 10(-12) to 1 x 10(-4) mol/L) to obtain dose-response curves. MEASUREMENTS AND MAIN RESULTS: High concentrations (> or = 3.3 x 10(-5) mol/L) of trilodothyronine produced dose-dependent relaxation of preconstricted rings. The relaxation was not selective for arteries or veins at arterial resting tensions, and with either potassium or phenylephrine as a vasoconstrictor. Propranolol had little effect on subsequent triiodothyronine-induced relaxation of potassium-constricted rings at resting arterial tensions. CONCLUSIONS: Triiodothyronine, in supraphysiological and suprapharmacological concentrations, dilates preconstricted rings of human blood vessels in vitro; however, triiodothyronine had no demonstrable vasomotor effects on human internal mammary artery or saphenous vein in clinically relevant concentrations (10(-9) to 10(-8) mol/L). Triiodothyronine administration in vivo most likely has little direct effect on the tone of human vascular smooth muscle, particularly coronary artery bypass conduits.

Blood Pressure↗

The hemodynamic effects of isotonic exercise using hand-held weights in patients with heart failure.

BACKGROUND: Controversy surrounds the use of resistance exercise in patients with heart failure because of concerns that increases in rate-pressure product and systemic vascular resistance might lead to increased afterload and decreased cardiac output. METHODS: Following pharmacologic left ventricular unloading therapy using a pulmonary artery catheter, 34 patients with advanced heart failure performed isotonic weightlifting exercise at 50%, 65%, and 80% of the calculated one repetition maximum. Measurements were made of hemodynamics, ST segment, rate-pressure product, serum norepinephrine, rating of perceived exertion, and dysrhythmias following each exercise set. RESULTS: Repeated analysis of variance showed significant increases in systolic blood pressure (p = 0.0005), diastolic blood pressure (p = 0.01), rate-pressure product (p = 0.005); serum norepinephrine (p = 0.004), and rating of perceived exertion (p = 0.0005). However, systemic vascular resistance and cardiac output did not change significantly (p>0.05). Pulmonary capillary wedge pressures, the incidence of dysrhythmias, and ST segments did not significantly differ from baseline. No patients experienced angina or dyspnea during the study. CONCLUSIONS: Isotonic exercise using hand-held weights was well tolerated hemodynamically and clinically, and no patients experienced adverse outcomes during exercise.

Adrenergic alpha-Agonists↗

Silk suture used in standard organ bath studies contracts upon exposure to Krebs buffer.

INTRODUCTION: Muscles of all types are routinely excised and studied under isometric conditions using force transducers in standard organ baths. In such studies, the muscle is stretched or "preloaded," as the magnitudes of the contractions evoked by various stimuli can vary markedly depending on this baseline parameter: many such studies refer to an optimal length and/or preload tension. While calibrating our equipment, we were surprised to find that the silk suture, which is commonly used in such studies, can generate considerable tension of its own, completely independent of any muscle tissue. METHODS: We compared two different types of silk suture in muscle baths using the standard organ bath technique, measuring baseline tension upon addition of various solvents/buffers. RESULTS: One type of silk suture was inert in that, upon stretching to a given degree, there was no important change in preload tension upon addition of bathing medium (Krebs buffer). The second type of silk suture, however, which is also widely available commercially, exhibited substantial contractile responses upon exposure to standard Krebs buffer solution, with magnitudes typically exceeding several grams force. This change developed over the first 30-60 min of exposure to Krebs, after which tension was stable. This change was not observed if the suture was presoaked for 60 min before hanging in the muscle bath, but was observed if the suture was allowed to dry again before use. Other solvents such as ethanol and DMSO did not alter tension. DISCUSSION: While this property of silk suture may be well known in the surgical setting, it is less well appreciated by other users of this material. This phenomenon is of major importance to any experimental study of muscle function, as it alters the preload tension under which such studies are carried out.

Buffers↗

Effects of age, testing speed, and arm dominance on isokinetic strength of the elbow.

The strength of active flexion/extension and supination/pronation was measured isokinetically in 60 right-hand-dominant nonathletic men. They were equally divided into a younger group (20 to 30 years) and an older group (50 to 60 years) with moderate occupational and spare-time activity levels. Peak torque, work, power, and the angle of peak torque production were measured bilaterally at a slow (90 degrees/sec) and a fast (180 degrees/sec) speed of movement. Although highly significant differences were found between the young and older groups in flexion and extension, no age-related differences were found in supination and pronation. The dominant side had significantly higher levels of peak torque, work, and power in flexion; however, no significant differences were detected in peak torque for extension, supination, and pronation. Isokinetic peak torque and work were greater at the slower speed as opposed to power, which was significantly greater at the faster speed with the exception of pronation movement. With regard to the angle where peak torque was achieved, significant differences were detected between groups, which suggests that age appears to affect where in the range of motion peak torque is produced.

Adult↗

The efficacy of stretching for prevention of exercise-related injury: a systematic review of the literature.

The objective of this study was to conduct a systematic analysis of the literature to assess the efficacy of stretching for prevention of exercise-related injury. Randomized clinical trials (RCTs) and controlled clinical trials (CCTs) investigating stretching as an injury prevention measure were selected. A computer-aided search of the literature was conducted for relevant articles, followed by assessment of the methods of the studies. The main outcome measures were scores for methodological quality based on four main categories (study population, interventions, measurement of effect, and data presentation and analysis) and main conclusions of authors with regard to stretching. One RCT (25%) and three CCTs (100%) concluded that stretching reduced the incidence of exercise-related injury. Three RCTs (75%) concluded that stretching did not reduce the incidence of exercise-related injury. Only two studies scored more than 50 points (maximum score=100 points) indicating that most of the studies selected were of poor quality. Neither of the two highest scoring RCTs showed positive effects for stretching. Due to the paucity, heterogeneity and poor quality of the available studies no definitive conclusions can be drawn as to the value of stretching for reducing the risk of exercise-related injury.

Athletic Injuries↗

Reliability of isokinetic strength measurements of the knee in children with cerebral palsy.

This study evaluates the reliability of isokinetic testing of the knee flexors and extensors in children with cerebral palsy (CP). Twelve children (seven girls, five boys), aged 9 to 15 years, participated in this study. The children's strength was measured bilaterally, using a dynamometer. Each participant was tested twice at an interval of 1 week. During each session, the participant performed five consecutive cycles of knee extension and flexion. Testing velocity was set at 90 degrees/s, and the range of motion was 80 degrees, starting with the knee flexed at 80 degrees and ending in full extension. The measured variable was the peak torque. Reliabilities were determined using intraclass coefficient (ICC) with two-way ANOVA model. The ICCs for individual sessions range from 0.90 to 0.99. ICCs for the eight repetitions over the two sessions range from 0.95 to 0.98. The findings indicate that measuring isokinetic strength in the tested population is highly reliable and should be considered in rehabilitation protocols.

Adolescent↗

Z/I and A-band lattice spacings in frog skeletal muscle: effects of contraction and osmolarity.

A-band and Z-line/I-band lattice spacings were measured by small-angle X-ray diffraction from relaxed and isometrically-contracting whole frog sartorius muscles with lattice spacings reduced or swollen by changing the osmolarity of the bathing solution. A-band spacing increased by approximately 3% upon isometric contraction at reduced lattice spacings (245-356 mOsm) and decreased by approximately 1% at swollen spacings (172 mOsm), similarly to the behaviour of skinned muscles upon changing from the relaxed state to rigor. The Z/I lattice underwent a significant lattice expansion (3-8%) upon isometric contraction at all osmolarities, in qualitative agreement (but quantitative disagreement) with results from electron microscopy on mammalian skeletal muscle. Lattice areas calculated for the Z/I and A-band lattices indicate a barrel-shaped sarcomere in the resting state, which may provide a partial explanation for how longitudinal forces produced in the A-band can produce a radial expansive force in the Z-line during contraction. The radial component of cross-bridge stiffness was calculated from the A-band data for contracting muscle, using a lattice stability model incorporating structural, osmotic and electrostatic forces. The calculations gave a radial cross-bridge stiffness during contraction of about 9 x 10(5) N m-2, and outward radial force per thick filament in normal Ringer's solution of 6 x 10(-9) N, corresponding to a radial force per cross-bridge of 10(-11) N.

Animals↗

Shortening properties of two biochemically defined muscle fibre types of the Norway lobster Nephrops norvegicus L.

Mechanical properties of myofibrillar bundles from single chemically skinned fibres from the superficial abdominal flexor muscle of the Norway lobster Nephrops norvegicus were measured, and the protein content of these fibres was analysed by SDS-PAGE. Two slow fibre phenotypes (S1, S2) were distinguished on the basis of their myofibrillar protein assemblages. Data from 9 S1 and 8 S2 fibres obtained at similar sarcomere length demonstrate significant differences between the fibre types in maximal tension (N cm-2, S1: 10.5 +/- 3.9; S2: 3.1 +/- 0.8), in the delay of the peak of stretch activation (ms, S1: 122 +/- 18; S2: 412 +/- 202), in fibre stiffness (N cm-2 per nm half sarcomere, S1: 0.36 +/- 0.19; S2: 0.09 +/- 0.03) and in maximal shortening velocity (fibre length s-1, S1: 0.53 +/- 0.10; S2: 0.27 +/- 0.06). Furthermore, the maximal power output of the type S1 fibres was about five times larger than that of S2 fibres. The power output was maximal at lower loads in S1 fibres (relative load = 0.37 +/- 0.04) than in S2 fibres (relative load = 0.44 +/- 0.05). This study represents a comprehensive investigation of two slow muscle fibre types which are thought to be specialized for slow movements (S1 fibres) and for the postural control of the abdomen (S2 fibres).

Animals↗

The N-methyl-D-aspartate-receptor antagonist dextromethorphan lacks analgesic effect in a human experimental ischemic pain model.

BACKGROUND: N-methyl-D-aspartate-receptor antagonists may be useful in pain management. The aim of this study was to evaluate dextromethorphan (DEX), a commonly used oral antitussive drug with NMDA-receptor antagonistic properties, in respect of its analgesic properties as single drug and co-administered with morphine (MO) on experimental ischemic pain. In addition, the analgesic effects of another clinically available NMDA-receptor antagonist, ketamine (KET) as well as of morphine (MO) were tested as active controls. METHODS: Nineteen healthy volunteers were included in the study. Experimental ischemic pain was induced using the forearm tourniquet test. Placebo (PLAC), oral DEX (30 and 90 mg, respectively), KET (9 microg kg(-1) min(-1) i.v.), MO (0.1 mg kg(-1), i.v.) and the DEX+MO and KET+MO combinations were evaluated during eight separate experiments. Development of ischemic pain was rated by visual analog scale (VAS) every minute over 30 min and ratings were summed as sum of pain scores (SPS). RESULTS: DEX by itself did not influence SPS compared to PLAC. The DEX+MO co-administration did not enhance MO-induced analgesia. MO and KET reduced pain ratings by 27% and 39%, respectively. The KET+MO combination showed no enhancement of the analgesic effect in comparison with the respective drugs in monotherapy. CONCLUSION: DEX in clinical doses has no effect on the present acute ischemic pain model and does not influence MO-induced analgesia. Further studies on other pain modalities are needed in order to evaluate the potential use of DEX in pain treatment.

Adult↗

Nitric oxide-dependent relaxation induced by M1 muscarinic receptor activation in the rat small intestine.

The aim of the present study was to investigate whether muscarinic M1 receptor activation induces intestinal relaxation via nerve-dependent nitric oxide formation. Mechanical activity in longitudinal segments of rat jejunum was recorded isotonically in organ baths. The muscarinic M1 receptor agonist 4-[[[(3-Chlorophenyl)amino]carbonyl]oxy]-N,N,N,-trimethyl-2-butyn- 1-ammonium chloride (McN-A-343, 10(-7)-10(-4) M) induced a concentration-dependent relaxation of rat jejunum. Relaxations induced by McN-A-343 (10(-5) M) were inhibited by the M1 receptor antagonist telenzepine (10(-8) M), and enhanced by the M3 receptor antagonist para-fluorohexahydrosiladifenidol (p-F-HHSiD; 3x10(-7) M). The inhibitory responses induced by McN-A-343 were abolished by the nitric oxide synthase inhibitors Nomega-nitro-L-arginine (L-NOARG; 10(-4) M) and Nomega-monomethyl-L-arginine (L-NMMA; 3x10(-5) M), the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ; 10(-5) M), and by tetrodotoxin (TTX; 3x10(-7) M). Guanethidine or hexamethonium did not affect inhibitory responses induced by McN-A-343. In conclusion, McN-A-343 induces nerve-dependent, nitrergic relaxations in rat jejunum, via activation of muscarinic M1 receptors. Hence, selective muscarinic M1 receptor agonists or antagonists might offer possibilities for pharmacological manipulation of the NO system.

(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethy↗

Gender variations of body composition, muscle strength and power output in morbid obesity.

BACKGROUND: Motor capabilities are reduced in obese (OB) individuals, and this impairment may result also from quantitative variation of muscle mass due to alterations in body composition. OBJECTIVE: This study aims to evaluate the differences in body mass (BM) and composition, as well as in muscle strength (ST) and power output W(.) between OB and NW males and females, and to test the hypothesis that variations in body composition affect muscle performance in OB subjects. DESIGN AND METHODS: Body composition (determined by BIA with a two-compartment model), upper and lower limb maximum ST (evaluated with isotonic machines) and lower limb maximum anaerobic W(.) (measured with a jumping test) were studied in a group of 95 extremely OB subjects (OB: 28 males, 67 females; mean age+/-s.d.: 29.3+/-7.0 y; BMI: 41.2+/-4.4 kg/m(2)) and in a control group of 18 NW voluntary subjects (NW: eight males, 10 females; age: 30.3+/-5.3 y; BMI: 22.6+/-2.1 kg/m(2)). RESULTS: OB male and female subjects differed significantly with increases in BM being attained by a similar contribution of fat mass (FM) and fat-free mass (FFM) in male subjects, but mainly contributed by FM in female subjects. Compared with NW, both OB men and women had a greater amount of FFM (P<0.001) and, since a general linear correlation was found between ST and FFM (ST (N)=64.4 FFM (kg)-190.0, R(2)=0.612, P<0.001), they developed higher values of ST (P<0.05) than their respective NW counterparts. For the same reason, both OB and NW male subjects had higher ST (P<0.001) than their female counterparts. Correction for FFM eliminated all gender- and obesity-related ST differences. On the contrary, in spite of their higher absolute muscle strength, both OB men and women could develop absolute W(.) similar to that of NW subjects, and were notably less powerful per unit BM than NW subjects (P<0.001), women being most affected among the OB. CONCLUSIONS: Obesity-related variation in body composition differs considerably by gender, and is responsible for differences in muscle performance: the higher muscle strength observed in OB subjects (both men and women) and in male subjects (both OB and NW) is accounted for by a greater amount of FFM. Nonetheless, biomechanical limitations appear to impair muscle power development during jumping in OB individuals.

Adult↗

The importance of posture on the isokinetic assessment of spasticity.

OBJECTIVE: To measure spasticity of the knee flexors and extensor muscles in two different hip positions. SETTING: Swiss Paraplegic Center Nottwil, Switzerland. METHODS: Twenty spinal cord injured (SCI) patients with complete lesions were tested with a torque-velocity dynamometer in the following positions: (1) supine with a hip angle of 0 degrees; (2) sitting with a hip angle of 90 degrees. The excursion of the knee was measured for both positions using a goniometer. Two flexion/extension movements of the knee were performed at a speed of 10 degrees per sec. A further four flexion/extension movements over the same trajectory were made at a speed of 120 degrees per sec. Eccentric peak torques were measured continuously during movement of both legs for both speeds. RESULTS: At a speed of 120 degrees per second, there was a significant difference in stretch reflex of the hamstrings and quadriceps femoris muscles for the two positions (Wilcoxon's paired t-test, P<0.05). Excitability was higher for the quadriceps femoris muscles in supine than in sitting position. For the hamstrings, the effect was reversed. Significant differences between sitting and supine position were not found for the speed of 10 degrees per sec. Test-retest reliability was high for the movements of 120 degrees per sec but low for 10 degrees per sec. CONCLUSIONS: Our findings indicate that for a reliable and comparable measurement of spasticity, an exact description of test position and procedure is essential.

Adult↗

Isotonic dynamometry for the assessment of power and fatigue in the knee extensor muscles of females.

Impairments in muscle power production and recovery following short-duration intense activity could lead to decreased performance and risk of injury. We developed a power test for the knee extensor muscles using torque-velocity testing and moderate isotonic loads. Twenty-eight female volunteers performed three maximal efforts at each of four isotonic loads (27.1, 40.6, 54.2 and 67.8 N. m). If the calculated regression line for the torque-velocity data had an r2 >/= 0.95 (i.e. an acceptable test), maximal power (408 +/- 56 W) was computed from the data. Immediately after torque-velocity testing, the subjects repeated maximal effort knee extensions with 33.9 N. m for three bouts of 15 repetitions with 15 s of rest to produce muscle fatigue, defined as a decrease in power output during isotonic exercise. After a 4 min rest, the torque-velocity test was repeated and power calculated (345 +/- 48 W). For the group, the recovery of maximal power after the fatigue protocol was 85%. The extremes were represented by one subject who recovered only 70% of her maximal power and another who recovered completely (>98%). Physiological differences in muscle power following repeated exercise could have an impact on the outcome of therapeutic interventions for sports injuries, fatigue syndromes and occupational over-use conditions.

Adult↗

Decreased muscle strength and contents of Mg and Na,K-pumps in chronic alcoholics occur independently of liver cirrhosis.

OBJECTIVES: To evaluate the influence of established liver cirrhosis on muscle strength and muscle contents of magnesium (Mg), potassium (K) and sodium, potassium pumps (Na,K-pumps) in chronic alcoholic patients. DESIGN: An open cross-sectional study. SETTING AND SUBJECTS: Forty consecutive chronic alcoholics (18 with cirrhosis and 22 without cirrhosis) admitted to the Department of Hepatology, Aarhus University Hospital, Denmark, or to a collaborating alcoholism treatment centre, and 36 healthy control subjects. MAIN OUTCOME MEASURES: Evaluation of participant's subjective physical ability and measurement of maximum isokinetic muscle strength and muscle mass, as well as measurements of Mg, K and Na,K-pumps in skeletal muscle. RESULTS: Maximum isokinetic muscle strength and muscle mass were equally reduced in patients with and without cirrhosis (P < 0.01 all). In keeping with this, both groups of patients felt equally physically restricted. Muscle Mg was reduced to the same extent in the two groups of patients (by 12 and 9%, P < 0.001, both), whereas the muscle K content was only significantly lower in the cirrhotic patients (10%, P < 0.001). The muscle content of Na,K-pumps was reduced by 14%, (P < 0.01) in the cirrhotic patients and by 8% (P < 0.05) in the noncirrhotic patients. CONCLUSION: Our alcoholic patients complained of physical disability, had reduced skeletal muscle mass, isokinetic muscle strength, content of muscle Mg and content of Na,K-pumps. There was no difference between patients with and without cirrhosis. It appears that it is the heavy alcohol intake, and not the cirrhosis per se, that is responsible for the observed defects.

Adult↗

[Dynamic 31-phosphorus magnetic resonance spectroscopy of the m. quadriceps: the effect of sex and age on the spectroscopic parameters].

PURPOSE: 31P-MRS is used to assess the influence of sex und age on quadriceps muscle metabolism before and after exercise. MATERIALS AND METHODS: 32 healthy volunteers (15 women, 17 men, mean age: 38 +/- 17 yrs.) were examined by dynamic phosphorus-31 (31P) magnetic resonance spectroscopy (MRS). In the magnet, the quadriceps muscle was stressed by an isometric und an isotonic form of exercise until exhaustion, respectively. RESULTS: Resting conditions: With increasing subjects' age, the ratio beta-adenosine triphosphate/total phosphate decreased (r = -0.37; p = 0.02). With increasing subjects' age, the ratios inorganic phosphate/phosphocreatine (r = 0.79; p = 5 x 10(-8), phosphomonoester/beta-adenosine triphosphate (r = 0.74; p = 10(-6) and phosphodiester/beta-adenosine triphosphate (r = 0.62; p = 10(-4) increased. The pH was the only one of the evaluated spectroscopic parameters which showed a sex-dependence: Female subjects had a significantly lower pH (7.03 +/- 0.02) than male subjects (7.05 +/- 0.03; p = 0.01). Exercise: With increasing age, the maxima of inorganic phosphate/phosphocreatine were less extreme during both of the exercises (r = -0.42; p = 0.0005). Likewise, the exercise-induced acidosis was less severe with increasing age (r = 0.53; p = 6 x 10(-6). After the end of the exercise, the times of half recovery of inorganic phosphate/phosphocreatine and the pH correlated neither with the subjects' age nor with sex or cross-sectional areas of the quadriceps muscle. CONCLUSION: Sex and age of volunteers affect spectroscopic results. This influence had to be considered in the interpretation of spectroscopic studies.

Adult↗

Warm-up, stretching and massage diminish harmful effects of eccentric exercise.

The effect of a combination of a warm-up, stretching exercises and massage on subjective scores for delayed onset muscle soreness (DOMS) and objective functional and biochemical measures was studied. Fifty people, randomly divided in a treatment and a control group, performed eccentric exercise with the forearm flexors for 30 min. The treatment group additionally performed a warm-up and underwent a stretching protocol before the eccentric exercise and massage afterwards. Functional and biochemical measures were obtained before, and 1, 24, 48, 72 and 96h after exercise. The median values at the five post-exercise time points differed significantly for DOMS measured when the arm was extended (p = 0.043). Significant main effects for treatment were found on the maximal force (p = 0.026), the flexion angle of the elbow (p = 0.014) and the creatine kinase activity in blood (p = 0.006). No time-by-treatment interactions were found. DOMS on pressure, extension angle and myoglobin concentration in blood did not differ between the groups. This combination of a warm-up, stretching and massage reduces some negative effects of eccentric exercise, but the results are inconsistent, since some parameters were significantly affected by the treatment whereas others were not, despite the expected efficacy of a combination of treatments. The objective measures did not yield more unequivocal results than the subjective DOMS scores.

Adult↗