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Allergic reactions due to glove-lubricant-powder in health-care workers.

Eight glove-wearing hospital personnel were evaluated for suspected type I-like allergic manifestations due to corn-starch powder. All subjects were clinically examined, the presence of atopy was assessed by administration of a questionnaire, the on-off test was verified (the clinical feature behavior was verified with regard to the beginning and the cessation of the work shift), levels of specific serum IgE for maize and latex were measured, and prick tests for the same allergens were performed. The on-off test was positive for everyone. The symptom associated with glove use was urticaria, which was also associated in one case with intermittent dyspnea and in another with oculorhinitis, angioedema, and asthma. Five workers were atopic. The serum IgE test found three positive responses to maize, three positive responses to both latex and maize, and two negative responses to both. However, in the two patients testing negative to IgE, the prick tests were positive: one for maize and the other for both maize and latex. All workers evaded further relapses by avoiding exposure to powdered gloves. There is general agreement that corn-starch powder may cause irritant dermatitis and that it may be a vehicle for other allergens. This study seems to suggest that corn-starch powder may act as a type I allergen itself. Further studies on a larger number of subjects and further research on the chemical properties of corn-starch powder, in particular on its protein content, are needed to confirm this hypothesis.

Adult↗

Nurses' infection-control practice: hand decontamination, the use of gloves and sharp instruments.

Infection is an acknowledged hospital problem. Micro-organisms are disseminated mainly via hands but there is evidence that hand decontamination, the most important means of prevention, is performed too seldom, and not always after activities likely to result in heavy contamination. Nurses themselves are exposed to risks of infection, chiefly through contact with blood and body fluids, yet it has also been reported that gloves are not always worn during contact with patients' secretions and that the handling and disposal of sharp instruments may be performed unsafely. The study reported in this paper documents nursing behaviour in relation to hand decontamination, the use of gloves and sharps, taking into consideration a number of variables which could influence practice: availability of the expertise afforded by an infection-control nurse, clinical setting, nursing workload, knowledge and the resources available to control infection. Hands were decontaminated after 28.78% of patient contacts. Hands were decontaminated after 49.85% of activities likely to result in heavy contamination. Performance was related to nursing workload and the availability of hand decontaminating agents, especially when the nurses became busy. Use of gloves when they were available also proved good, with little evidence of wasteful use. The handling and disposal of sharps were commendable for most subjects but a few grossly unsafe incidents were nevertheless witnessed, apparently not associated with any of the variables examined.

Analysis of Variance↗

Cross-infection control: attitudes of patients toward the wearing of gloves and masks by dentists in the United Kingdom in 1987.

Dental patients in the United Kingdom were surveyed in February 1987 with respect to their views on whether dentists should wear gloves and/or masks. Most patients believed that gloves should be worn and few saw no need for either. Patients receiving care in a dental hospital were more enthusiastic than those seen in general practice in the belief that dentists should wear gloves and/or a mask.

Attitude to Health↗

Implications of the presence of dithiocarbamate in latex gloves.

Dithiocarbamate is used as a preservative or vulcanizing accelerator in the manufacturing process of latex gloves. Twenty-five types of gloves were tested for the presence of this additive by three methods: using an iodide/azide reagent; elemental analysis; and investigating the inhibition of the setting reaction of a vinyl polysiloxane putty. The results of these tests confirm that dithiocarbamates are the chemical components which cause the inhibition of the setting of a vinyl polysiloxane putty. Given this finding, and concerns regarding the possible effects of dithiocarbamate on skin, the use of dithiocarbamate-free gloves is recommended.

Dental Impression Materials↗

Application of the hygienic hand-disinfection test to the gloved hand.

The Austrian Standard Hygienic Hand-Disinfection Test was adapted for comparing the effect of washing artificially contaminated hands (using Escherichia coli) with contaminated gloved hands, using liquid soap and rinsing with water. Tests showed that a single soap wash completely removed all the bacteria from the glove, and was more than 1000 times more effective on the glove than on the hand.

Escherichia coli↗

Contamination of surgeons' glove fingertips during surgical operations.

The external surfaces of surgeons' gloves were examined for contamination by bacteria during the course of surgical operations. Of 31 operations studied the surgeons' glove surfaces yielded bacteria at the end of the operation in 16 cases. Bacterial contamination increased during the course of the operation. The surgeons' fingertip skin and nail beds were cultured after surgical hand scrub and drying, but before donning gloves; bacteria were recovered from these sites in 10 of 21 and 13 of 21 cases, respectively.

Colony Count, Microbial↗

A new powder-free glove with a textured surface to improve handling of surgical instruments.

A latex surgical glove has been designed to have a textured surface that stimulates the surface of intact skin. This textured surface enhances security of grasp of surgical instruments under wet and dry conditions without altering tactile discrimination. The unique hydrogel polymer of this glove allows the glove to be donned with either wet or dry hands without the use of cornstarch lubricants.

Evaluation Studies as Topic↗

Influence on grip of knife handle surface characteristics and wearing protective gloves.

Ten subjects were asked to apply maximum torques on knife handles with either their bare hand or their hand wearing a Kevlar fibre protective glove. Four knife handles (2 roughnesses, 2 hardnesses) were tested. Surface electromyograms of 6 upper limb and shoulder muscles were recorded and subject opinions on both knife handle hardness and friction in the hand were also assessed. The results revealed the significant influence of wearing gloves (p<0.0001), knife type (p<0.0005) and handle hardness (p<0.005) on the applied torque. Wearing Kevlar fibre gloves greatly increased the torque independently of the other two parameters. Under the bare hand condition, a 90 degrees ShA slightly rough handle provided the greatest torque. Subject opinion agreed with the observed effects on recorded torque values except for the hardness factor, for which a preference for the 70 degrees ShA value over the 90 degrees ShA value emerged.

Adult↗

Surgical-glove-related diffuse lamellar keratitis after laser in situ keratomileusis: long-term outcomes.

PURPOSE: To study the long-term refractive results in eyes that developed surgical-glove-related diffuse lamellar keratitis (DLK) after laser in situ keratomileusis (LASIK). SETTING: Department of Ophthalmology, Hospital Provincial, Toledo, Spain. METHODS: This retrospective review analyzed an epidemic of surgical-glove-related DLK over a 5-month period at a single hospital. Twenty-four eyes (24 patients) that developed DLK (DLK group) were compared to 30 eyes (30 consecutive patients) that had surgery during the same time but had an uneventful postoperative course (control group). Follow-up was 12 months in all cases. RESULTS: Twelve months after LASIK, the mean spherical equivalent was 0.14 diopter (D) +/- 0.36 (SD) in the DLK group and -0.07 +/- 0.33 D in the control group (P=.03). The mean uncorrected visual acuity was 0.91 +/- 0.18 and 0.90 +/- 0.17, respectively (P = .81). The mean best spectacle-corrected visual acuity (BSCVA) was 0.97 +/- 0.08 in the DLK group and 0.99 +/- 0.06 in the control group (P = .42). At 1 year, 91.7% of eyes in the DLK group and 93.3% of eyes in the control group were within +/-0.50 D of the attempted correction (P = .82). The BSCVA was 1.0 or better in 87.5% and 93.3%, respectively (P =.46). CONCLUSIONS: Early diagnosis and appropriate treatment of glove-related DLK provided visual outcomes that were similar to those in eyes with an uneventful postoperative course. These good results are consistent with those in studies of classic DLK.

Adult↗

The self-retracting glove--an aid to minilaparotomy.

A simple technique is presented to help the surgeon gain additional exposure during open minicholecystectomy or any other open minimal abdominal procedure. The technique consists of a empty sterile glove inserted into the abdomen and filled with 500 mL saline. When filled with saline, the glove becomes soft and malleable-adjusting itself to fill the desired space. The resulting retraction is nontraumatic to the adjacent organs. The small laparotomy wound is spared the trauma from the deep retractors that would have been otherwise used to achieve exposure. The retracting glove is effective, easy to use, inexpensive, and safe.

Cholecystectomy↗

Gloved hand as applicator of antiseptic to operation sites.

A 95% ethanol solution containing 0-5% chlorhexidine digluconate caused a significantly greater mean reduction in skin bacteria (99-9% plus or minus 0-024) when rubbed by a gloved hand on to the skin of one hand for two minutes than when applied to the same area for the same time with the traditional gauze applicator for operation sites (90-7% plus or minus 2-12). The latter reduction, however, was greater than that reported in five previous experiments in which application of the same solution for the same time to two hands gave mean reductions varying from 79% to 84%. An aqueous solution and a 70% alcoholic solution of chlorhexidine also gave significantly greater reduction (and alcoholic povidone iodine almost significantly greater reduction) when applied by a gloved hand than on gauze. It is inferred that the effectiveness of skin disinfection depends both on the antiseptic used and on the manner of application, and varies with the amount of friction used in applying the antiseptic. Further studies on disinfection of the surgeon's hands with 0-5% chlorhexidine in 95% alcohol rubbed on and allowed to dry have supported its value and acceptability; the mean bacterial counts of washings from gloves of surgeons after operations were lower after the use of this method than after other preoperative preparations of the hands.

Bacteria↗

A study of the use of gloves in a large teaching hospital.

A study was conducted in the wards and laboratory of a 900-bed teaching hospital to determine the appropriateness of glove use. In the wards the rate of appropriate use was 59%, and in the laboratory it was 90%. In general, in the patient areas gloves were used for specific tasks (70%) whereas in the laboratory use for a specific task was only 32%. Financial savings could be realized by educational programs in infection control directed toward a more rational use of gloves.

Attitude of Health Personnel↗

Powder-free gloves for ophthalmic surgery.

Powdered and powder-free gloves were compared during ophthalmic surgery. It was found that powder may reappear on well-rinsed gloves by the end of intraocular surgery and be implanted inadvertently into the eye or in the stromal interface during laser in situ keratomileusis. Powder-free gloves, therefore, make surgery significantly safer.

Cornea↗

Nursing wound care survey: sterile and nonsterile glove choice.

PURPOSE: The application of sterile and clean procedure to the practice of wound care nursing was examined. DESIGN: This prospective, descriptive study surveyed staff nurses regarding glove use. SUBJECTS AND SETTING: Seven hundred forty-three staff nurses from five health care agencies in the San Francisco Bay Area responded to the survey. INSTRUMENTS: A self-report wound care survey instrument was developed by Nursing Consortium for Research and Practice members from information adapted from the wound care literature. The questionnaire comprised 31 questions and required approximately 10 minutes to complete. METHODS: Nursing Consortium for Research and Practice members obtained approval from their respective institutional human subjects committees and distributed questionnaires among all nurses engaged in direct care. Some agency representatives personally handed the survey instruments to subjects, but most distributed them through their agencies personnel mailing systems. RESULTS: Seven hundred twenty-three (38%) of 1900 questionnaires were completed and returned to the five site coordinators. Differences were found between acute care and home health nurses. Acute care nurses were more likely than home care nurses to use sterile gloves in all wound care situations. CONCLUSION: Greater variation was found with regard to sterile technique in wound care practice than in previously reported studies. Although patient risk factors and wound type significantly influenced the choice of sterile or clean gloves, additional environmental and personal factors exerted considerable influence. These included health care setting, degree of professional education, and nurses' experiential background. Attempts to modify practice through policy change alone may not be sufficient to overcome resistance to change. Instead, it may be necessary for nurses to "unlearn" lessons from basic nursing education before they can adopt to new practices and clinical policies.

Adult↗

The use of gloves in cross-infection control. A historical note.

Routine glove wearing has been an integral part of surgery for almost a century, but it has only recently been considered appropriate for dentists and their staff while treating patients. This paper traces the history of glove use in surgery and compares this with experience of glove use in dentistry at the present time.

Communicable Disease Control↗

A practical gloving and handwashing regimen for dental practice.

The debate concerning the re-use of gloves in dental practice continues, despite the fact that recent guidelines on cross-infection control recommend that a new pair of gloves should be used for each patient. Whichever policy for glove use is adopted, it is important to take the maximum possible precautions in hand care. This paper presents a practical regimen for hand/glove washing which is simple, quick and effective. The importance of establishing a hand-care regimen in dental practice is emphasised.

Cross Infection↗

Specific monoclonal antibodies and human immunoglobulin E show that Hev b 5 is an abundant allergen in high protein powdered latex gloves.

BACKGROUND: Hev b 5 is a major latex allergen recognized predominantly by latex-allergic health care workers (HCWs). Recombinant Hev b 5 (rHev b 5) was previously expressed as a fusion protein with maltose binding protein (MBP), itself an immunogenic molecule; therefore non-fusion rHev b 5 is desirable. Moreover, standardized immunological assays for the detection of Hev b 5 are currently lacking and may have important implications for both allergen avoidance and diagnosis in latex allergy. OBJECTIVES: To generate and use Hev b 5-specific mAbs to determine the relative abundance of Hev b 5 in different latex extracts, correlating this with the IgE reactivity of latex-allergic HCWs and to produce non-fusion rHev b 5. METHODS: For the production of mAbs, mice were immunized with rHev b 5/MBP fusion protein and mAbs selected with rHev b 5/MBP but not MBP reactivity. The mAb reactivity was compared with polyclonal IgE from latex-allergic HCWs using direct and inhibition ELISA and immunoblot assays. Recombinant Hev b 5 was expressed and purified in the pPROEX-HTa bacterial expression system. RESULTS: Four Hev b 5-specific mAbs were produced. Immunoblotting and ELISA using the mAbs indicate abundant Hev b 5 in high protein powdered latex glove extracts as compared with crude latex sap extracts. High quality surgical gloves with no detectable protein have no detectable Hev b 5. Inhibition ELISAs using serum IgE from latex-allergic HCWs and Hev b 5-specific mAbs gave strong correlation. Non-fusion recombinant Hev b 5 was successfully expressed and purified, showing reactivity with both the Hev b 5-specific mAbs and serum IgE of latex-allergic HCWs. CONCLUSION: Hev b 5-specific mAbs and human IgE from latex-allergic HCWs demonstrate the greater content of Hev b 5 in high protein powdered glove extracts. This may explain the observed higher frequency of sensitization to this allergen in HCWs.

Allergens↗

Bacterial contamination of surgical gloves by water droplets spilt after scrubbing.

Wound infection and deep sepsis can have disastrous consequences, particularly in orthopaedic surgery. Strict protocols, ultra-clean air, prophylactic antibiotics, and impervious gowns and drapes, have all been shown to diminish wound infection. However it remains a common and significant problem. The water droplets spilt from the surgeons hands after meticulous scrubbing with povidone iodine were cultured. The permeability of the surgical glove packaging to Gram-positive bacteria was also investigated. The water droplets from the surgeon's arms contained environmental and potentially pathogenic bacteria including a micrococcus, a coliform and coagulase-negative staphylococci. The paper packaging for the range of sterile surgical gloves tested was discovered to be permeable to Gram-positive bacteria. In conclusion accidental water droplet contamination of surgical gloves is a potential source of infection. Alternative recommendations are made.

Equipment Contamination↗