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Complex organic chemical balms of Pharaonic animal mummies.

Millions of votive mummies of mammals, birds and reptiles were produced throughout ancient Egypt, with their popularity increasing during the reign of Amenhotep III (1400 bc) and thereafter. The scale of production has been taken to indicate that relatively little care and expense was involved in their preparation compared with human mummies. The accepted view is that animals were merely wrapped in coarse linen bandages and/or dipped in 'resin' before death. However, as with human mummification there was a range of qualities of treatments, and visual inspection of animal mummies suggests that the procedures used were often as complex as those used in humans (for example, evisceration and elaborate bandaging). Moreover, the ancient Egyptians treated animals with great respect, regarding them both as domestic pets and representatives of the gods; for example, the cat symbolized the goddess Bastet; the hawk, Horus; the ibis, Thoth, and so on. We report here the results of chemical investigations of tissues and wrappings from Pharaonic cat, hawk and ibis mummies using gas chromatography, gas chromatography-mass spectrometry, thermal desorption-gas chromatography-mass spectrometry and pyrolysis-gas chromatography-mass spectrometry. The analyses reveal the presence of highly complex mixtures of n-alkyl and cyclic biomarker components characteristic of fats, oils, beeswax, sugar gum, petroleum bitumen, and coniferous, Pistacia and possibly cedar resins. The mixture of balms is of comparable complexity to those used to mummify humans from the same period.

Animals↗

[Radiological investigations of over-modelled skulls from New Ireland (Melanesia) (author's transl)].

The authors examined a series of overmodelled and decorated skulls from New Ireland which had been used as cult objects for ritual dances. The results are described briefly with a number of examples. Radiological examination provided no evidence that these had been chosen specifically according to age or sex. Of particular significance for ethnology is the finding of two skulls in which the jaws have been replaced before modelling by adapted pigs' mandibles. The non-destructive radiological investigation of artistic and cultural objects has a particularly long tradition in Berlin (eg. Baessler). With these examples we should like to stimulate further similar work.

Adult↗

Short-term effects of formaldehyde on peak expiratory flow and irritant symptoms.

The authors studied the respiratory effects of formaldehyde exposure among students who dissected cadavers in a gross anatomy laboratory. Peak expiratory flow and respiratory symptoms were measured before and after each weekly laboratory session. Each of 38 students was exposed to formaldehyde for 2.5 hr/wk for 14 wk. Individual, daily formaldehyde measurements averaged 1.1 ppm (standard deviation = 0.56 ppm). Multivariate models demonstrated two different time scales of effect of formaldehyde on peak expiratory flow: (1) exposure during the previous 2.5 hr reduced peak expiratory flow by -1.0% per ppm, and (2) average exposure during all preceding weeks reduced peak expiratory flow by an additional -0.5% per ppm of formaldehyde. However, the short-term exposure effect was diminished during the first 4 wk, suggesting at least partial acclimatization. Symptom reporting was also associated with exposure during the previous 2.5 hr, and similar evidence of acclimatization was observed. These results suggest that there are two different time scales of response to formaldehyde, and they emphasize the need for longitudinal studies, characterized by quantitative exposure characterization, and frequent measurements of outcome.

Acclimatization↗

The production of amitriptyline from nortriptyline in formaldehyde-containing solutions.

The stability of nortriptyline in aqueous solutions containing various concentrations of formaldehyde was investigated. Amitriptyline, as a reaction product, was determined by gas chromatography/mass spectrometry (GC/MS) in these experiments. Factors that may contribute to this phenomenon, including pH, formaldehyde concentration, and incubation time were evaluated. At 40% (v/v) formaldehyde concentration and pH 4, there was a 68% decrease in nortriptyline concentration along with a concomitant formation of amitriptyline after 24 h. The N-methylated product was responsible for 48% of the total tricyclic drug present. The data also clearly indicate that the formation of amitriptyline is favored at elevated pH.

Amitriptyline↗

Methylation of 3,4-methylenedioxymethamphetamine in formalin-fixed human liver tissue.

3,4-Methylenedioxymethamphetamine (MDMA or ecstasy) is a commonly consumed recreational drug. As is the case with most secondary amines, MDMA reacts with formaldehyde under acidic conditions to form tertiary amines. This reaction is likely to occur in formalin-fixed tissue. In formalin solutions, MDMA is methylated producing 3,4-methylenedioxy-N,N-dimethylamphetamine (MDDA). MDDA standard was synthesized by treating methylenedioxyamphetamine HCl in formaldehyde solution. Structure confirmation was by electrospray ionization-mass spectrometry (MS) and MS-MS. Randomly chosen human liver pieces (100-200 mg) were injected with 2 microg of MDMA HCl. The liver pieces in centrifuge tubes were covered with 200 microL of formalin solution (20% v/v), held at room temperature for 24 h, and then homogenized. The resulting suspension was sonicated for 5 min and then centrifuged. Controls consisted of substitution of 200 microL of water in place of formalin solution. Supernatant aliquots (10 mciroL) were added to 500 microL of 0.1% formic acid in acetonitrile for MS analysis. Positive ion electrospray spectra recorded in MS, MS2, and MS3 modes were used to confirm the presence of methylated MDMA. Liver tissue containing added MDMA HCl but not treated with formalin did not show a detectable level of methylated MDMA.

Embalming↗

Experimentally induced upper facial third fractures in unembalmed human cadaver heads.

Considerable need exists in the transportation industry to develop safety guidelines to protect the head and neck. One of the goals of this study was to produce facial fractures similar to those induced in motor vehicle crashes. Unembalmed cadaver heads were fixed to a supporting device and impacted with a steel pipe. The most common fracture was of the frontal sinus; multiple orbital wall, naso-orbitoethmoid, Le Fort I, II, and III fractures were also produced. Average impact speeds of 7.2 meters per second striking at the supraorbital rims created severe injury to both skull and contents. Energy absorption values accounted for the actual total contact time between head and pipe with tolerance level values measuring the force at specific intervals. The method described may be used to reproduce reliably those forces resulting in the facial fractures seen in the emergency room setting after motor vehicle crashes.

Acceleration↗

Diagnostic challenges in Creutzfeldt-Jakob disease: case report.

Sporadic Creutzfeldt-Jakob disease (CJD) is the most common prion disease. The diagnosis can be confirmed only by histological examination of brain tissue obtained at biopsy or at autopsy. Because of the transmissible nature of the disease, autopsy or brain biopsy cannot be performed at many institutions, which poses numerous challenges in confirming the diagnosis. We report the case of a patient with CJD in which autopsy to confirm the diagnosis was performed after overcoming numerous obstacles and advocating with hospital leadership. This case illustrates the numerous challenges that exist in achieving a definitive diagnosis of CJD and in postmortem disposition of the body, and we provide recommendations to clinicians who face similar challenges.

Aged↗

The vascular pattern of the human dorsal root ganglion and its probable bearing on a compartment syndrome.

STUDY DESIGN: A descriptive anatomic investigation of the vasculature of the dorsal root ganglions. OBJECTIVES: To determine whether the blood supply of the various spinal ganglions is sufficiently consistent to derive a "generic" description and illustration that would be applicable to all spinal levels, and to ascertain whether this vascular pattern is inherently predisposed to the development of a closed compartment syndrome. SUMMARY OF BACKGROUND DATA: The few previous descriptions of spinal ganglionic vasculature do not include photographic evidence showing uniformity in the arterial distribution plan at all ganglionic levels. The venous drainage, although verbally reconstructed from microscopic sections, lacks any indication of its probable role in the etiology of a compartment syndrome. METHODS: Three perinatal cadavers received latex/India ink injections, and their removed radiculomedullary systems were cleared, transilluminated, and macroscopically photographed. Paravertebral sections were grossly removed from the spines of two adult anatomic cadavers and received retrograde venous injections of a fine suspension of barium sulfate. The intervertebral foraminal tissues were then dissected from the bone, and radiographs of them were made. For comparative reference, a nerve root/ganglion complex of a rabbit was arterially injected with a more dilute preparation of the latex/India ink suspension. RESULTS: Macroscopic photographs of perinatal dorsal root ganglions showed that the pattern of the intraganglionic arterial distribution was sufficiently consistent to allow a graphic rendering and labeling of a "generic" ganglion. The series of incomplete retrograde venous injections adequately indicated the pressure labile location of a periganglionic venous plexus. CONCLUSIONS: The common development, structure, and function of the human dorsal root ganglions have resulted in the evolution of a uniform nutritional vascular pattern that can be conceptualized in a single visual image. Its plan of a primarily internal arterialization with a superficial venous drainage renders it vulnerable to the ischemic conditions consequent on external pressures and/or internal edematous swelling. This vascular arrangement may contribute to a propensity for the ganglion to develop a compartment syndrome when subjected to compression by periforaminal degenerative or neoplastic space-occupying lesions.

Adult↗

Nuclear magnetic resonance (NMR) relaxation spectroscopy in tissues.

Nuclear magnetic resonance (NMR) techniques have been used to study several in vivo and stabilized tissue samples. The results show that a multicomponent behavior characterizes the magnetization relaxation of all investigated samples. Various specimens were allowed to undergo necrotic processes and then they were examined with the same techniques used in in vivo tissue. A marked approach toward a single-exponential magnetization-decay behavior was observed in these necrotic samples. In addition, large and sometimes irreversible temperature variations have been observed in the relaxation parameters of several preserved and stabilized tissue samples. The most dramatic changes observed throughout these experiments relate to the long-lived magnetization components, and are not observed in in vitro experiments performed solely on necrotic tissues. Meaningful differentiation between normal and neoplastic tissue by NMR techniques is virtually useless unless the roles of the temperature and necrosis on the different magnetization-decay components are well understood.

Animals↗

Three-dimensional analysis of the arterial pattern of the upper limbs in Lorisiformes (Prosimiae, Primates).

A study of the arterial patterns of the upper limbs in Lorisiformes (Prosimiae), especially those of the rete mirabile and their correlating arteries, was carried out by three-dimensional arteriographic analysis. The rete displayed at least two types of plexiform distribution: (1) a vascular bundle in Lorisidae, which consists of a division of the main artery into a principal trunk surrounded upwards by about 50 small arteries, and (2) a simple network pattern in Galagidae, which, at most, is composed of simple vascular networks of the nature of a few widely scattered anastomoses. The arterial patterns of the upper limb in Lorisiformes are basically the same, but the position of divergence and the number and size of the vessels in the rete differ. The arterial patterns of the upper limbs in Lorisiformes closely resemble the development of the vessels in the 21- to 23-mm stages of human embryonic growth. In this study, we observed some similarity in the patterns between the rete mirabile and the superficial brachial artery in man.

Angiography↗