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Molecular biological characterization of equine herpesvirus type 1 (EHV-1) isolates from ruminant hosts.

Isolates of equine herpesvirus type 1 (EHV-1) originating from affected antelope and cattle were compared with reference EHV-1 and EHV-4 isolates and were characterized. Based on cross-neutralization, DNA restriction profiles and blot-hybridization data these isolates could be characterized as EHV-1. One isolate (from an antelope) with a different restriction profile showed significant DNA homology with EHV-1, partial homology with EHV-4, and little or no homology with EHV-2 and HSV-1 DNAs. Blot hybridization revealed differences in DNA restriction fragments located at the termini of two isolates and size heterogeneity in the unique long/internal repeat junction fragment (UL/IR) of one isolate.

Animals↗

The relationship between rate of oxygen consumption, heart rate and thermal conductance of the dik-dik antelope (Rhynchotragus kirkii) at various ambient temperatures.

1. The extent of cardiovascular adjustments to heat and cold were investigated between ambient temperatures of 5 and 45 degrees C by measuring conductance and the rates of oxygen consumption and heart beats. 2. Minimum heart rate was observed at 25 degrees C (114 +/- 9 beats/min). In the heat at 45 degrees C heart rate was observed to increase only slightly (127 +/- 12 beats/min) but in the cold -5 degrees C heart rate nearly doubled that at 25 degrees C. 3. Thermal conductance was on average 0.031 mlO2 (g. hr. degrees C)-1 below 25 degrees C but increased by more than 20 times at 40 degrees C. 4. A positive correlation between heart rate and rate of oxygen consumption was demonstrated below 25 degrees C and the relation may be of practical use.

Adaptation, Physiological↗

Molar proportions of volatile fatty acids in the gastrointestinal tract of East African wild ruminants.

The molar proportions of seven individual VFA's were determined at select sites along the gastrointestinal tract of sixteen species of East African wild ruminants. The resulting data were statistically analyzed for species effect, and for effects due to major feeding groups (browsers, grazers, fresh grass grazers, etc.) and for body weight groups (5-750 kg animals). Present data suggest that body weight, rather than diet, is the more influential factor in reticulo-rumen fermentation rate, and in the molar proportion of fatty acids present. The molar proportions of VFA's observed in the mid and hindgut of these wild ruminants appeared more responsive to diet and body weight of the animal than did foregut VFA values.

Animal Population Groups↗

Digestive physiology of East African wild ruminants.

The physiology of digestion of wild ruminants in their natural habitat was determined. The study consisted of fifty-one adult male animals representing sixteen species. While diet and body weight were both related to the gastrointestinal composition, body weight appeared to be the more influential factor. Reticulo-rumen and caecal-colon composition, relative to abomasal and small intestinal composition, showed the greater species, diet and body weight effects. The buffalo, oryx and gerenuk were somewhat more unique in gastrointestinal composition than were other species of wild ruminants, and were deserving of special consideration.

Animal Population Groups↗

Basic haematological values in antelopes--II. The Hippotraginae and the Tragelaphinae.

Basic haematological values in 49 animals of five species of the subfamily Hippotraginae, namely the roan antelope Hippotragus equinus, sable antelope Hippotragus niger, adax antelope Addax nasomaculatus, gemsbok oryx Oryx gazella gazella and scimitar horned oryx Oryx damah and in 51 individuals of five species in the subfamily Tragelaphinae, including the bushbuck Tragelaphus scriptus, nyala Tragelaphus angasi, greater kudu Tragelaphus strepsiceros, cape eland Tautotragus oryx and bongo Bocercus euryceros are reported. The erythrocyte counts were in the range of 12.62 X 10(12)/l in the gemsbok oryx to 6.44 X 10(12)/l in the bongo, the haematocrit values varied from 0.488 in the nyala to 0.380 in the roan antelope and for the haemoglobin highest levels (164.0 g/l) were noted in the adax antelope, and the lowest (105.5 g/l) in the bongo. Leukocyte counts were found mostly in the normal human range and varied from 7.17 X 10(9)/l in the adax antelope to 4.05 X 10(9)/l in the nyala, only in the greater kudu decreased values of 3.02 X 10(9) were estimated. These results are compared with findings taken from the literature, and with the normal human range.

Animals↗

Basic haematological values in antelopes--III. The Reduncinae and the Antelopinae.

Basic haematological values were measured in 50 individuals of five species falling under the subfamily Reduncinae, including the mountain reedbuck (Reduncula fulvorufula), the eastern waterback (Kobus ellipsiprymnus ellipsiprymnus), the defassa waterbuck (Kobus ellipsipyrmnus defassa), the lechwe waterbuck (Kobus leche) and the Lady Gray's waterbuck (Kobus megaceros) and in 45 animals of three species of the subfamily Antelopinae, including the springbok (Antidorcas marsupialis), the Grant's gazelle (Gazella Granti) and the dama gazelle (gazella dama). The highest mean erythrocyte counts were found in the male springbok: 11.72 X 10(12)/l, the lowest in the lechwe waterbuck: 8.39 X 10(12)/l, the mean haematocrit values varied from 0.538 in the lechwe waterbuck to 0.443 in the defassa waterbuck and the mean haemoglobin content ranged from 181.2 g/l in the male springok to 151.8 g/l in the defassa waterbuck. Leukocyte counts were mostly shown to be in the human range, in the subfamily Reduncinae at the lower limit; only in the mountain reedbuck decreased values of about 3.5 X 10(9)/l were demonstrated. These results are compared with information from the literature and with the normal human range.

Animals↗