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Heat stress induced enhancement of heat shock protein gene activity in the honey bee (Apis mellifera).

We employed in vitro translation of mRNA and product separation using SDS-PAGE to examine the heat-shock response of the worker honey bee. Increases in the levels of 6 translatable RNA populations were observed following heat stress. The greatest response was observed among bees aged 9 days. Slight levels of induction of 70 and 82 kDa heat shock proteins were evident among bees taken directly from the colony.

Age Factors↗

Polymorphisms in mitochondrial DNA of European and Africanized honeybees (Apis mellifera).

This study demonstrates polymorphisms in both the length and in the restriction enzyme cleavage sites of honeybee mitochondrial DNA (mtDNA). The levels of variation are typical of those found in other metazoan species. These polymorphisms are potentially useful for the identification of Africanized bees in the western hemisphere and for study of honeybee phylogenetics.

Animals↗

Correlations between glucose-inhibition and control parameters of alpha-glucosidase kinetics in Apis mellifica haemolymph (Hymenoptera: Insecta).

Kinetics studies of haemolymph alpha-glucosidase inhibition by D. glucose led to general correlations between inhibition and control parameters, all over the honeybee development. Maximum velocities are not affected by the inhibition, while affinity constants are always significantly increased, and Hill coefficients tend to decrease, especially in for-aging adults and in prenymphs. In this later case, the 'n-type' effect tends to break the manifestation of the 'K-type' mechanism.

Animals↗