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PubMedDate not supplied

[Autotrophic synthesis of polyalkanoates by Alcaligenes eutrophus in presence of carbon monoxide].

The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol% CO did not inhibit the key enzymes of PHA synthesis--beta-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt% PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol% beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Alkanes↗
PubMed2000

[Effects of nitrogen feeding on the accumulation of poly-beta-hydroxybutyrate with Alcaligenes eutrophus].

On the basis of analysis of PHB fermentation processes, the effects of ammonium sulfate feeding rate at PHB formation period on the PHB accumulation by Alcaligenes eutrophus were investigated. It was shown that the complete absence of nitrogen source at PHB formation phase would lead to the decline of PHB synthetic activity, and the obvious influences of different nitrogen feeding rate on PHB synthesis were observed. Higher PHB content, but relative lower cell dry weight, PHB concentration and PHB productivity could be obtained at slower nitrogen feeding rate. The excessive nitrogen feeding rate resulted in the drop of PHB content, which led to the decrease of PHB concentration and PHB productivity. The better results could be achieved when the ammonium sulfate feeding rate was set at around 0.5 g/h.

Ammonium Sulfate↗
PubMed1999

[Optimization of fermentation conditions for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Alcaligenes eutrophus].

A close relationship, between the initial addition time and concentration of propionate and HV fraction, was observed in shaking culture of Alcaligenes eutrophus for the production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The optimal initial addition time of propionate was determined at the onset of PHBV formation period. Although relatively high HV unit could be obtained under high propionate concentration, the growth and product synthetic activities were inhibited obviously. Different ratios of glucose to propionate were fed to stimulate the formation of HV unit and the results were compared. The optimizing feeding strategy of propionate was proposed based on the consideration of several fermentation index. The final cell dry weight, PHB concentration, PHB content and HV fraction in PHBV reached 52.1 g/L, 40.8 g/L, 78.3% and 16.2 mol%, respectively, Yield coefficient of HV unit to propionate and PHBV productivity were obtained to be 0.5 g/g and 0.74 g/(L/h), respectively.

Cupriavidus necator↗
PubMedDate not supplied

[Purification and study of the properties of a desulfonating enzyme in Pseudomonas alcaligenes].

An enzyme desulfonating anionic alkyl benzene sulfonate (ABS) surfactants was isolated from Pseudomonas alcaligenes TR and purified. The physicochemical and catalytic characteristics of the enzyme were studied. The kinetic constants of ABS desulfonation were determined and shown to depend on the length of a hydrocarbon radical. The molecular mass of the enzyme was found to be close to 60,000.

Benzenesulfonates↗