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PubMed1986

Presence of enkephalin precursor in molluscan neural tissue extract.

Mytilus pedal ganglia extract was treated sequentially with TPCK-trypsin and carboxypeptidase B. The treated sample and an untreated sample were purified separately with Sep-Pak C18 and subjected to HPLC. Fractions with Rt's corresponding to the Met-, Leu-enkephalin and Met-enkephalin-Arg6-Phe7 were assayed for enkephalin activities by displacement studies using pedal ganglia membrane and 3H-DAMA. The data showed the activities in the regions of Met-, Leu-enkephalin and Met-enkephalin-Arg6-Phe7 of the treated sample increased over that of the untreated sample by 2.3, 2.5 and 1.6 folds respectively. These results provided strong evidence for the presence of enkephalin precursor material in Mytilus.

Animals↗
PubMedDate not supplied

[UMP synthase activity of rat tissue extracts in the bone marrow form of radiation sickness].

Whole-body gamma-irradiation of rats with a dose inducing bone marrow radiation syndrome caused phase organospecific changes in UMP-synthase activity. Disturbances of enzymic activity in the bone marrow and spleen well correlated with the dynamics of interphase and reproductive cell death. In brain extracts, UMP biosynthesis from orotic acid did not undergo essential changes.

Animals↗