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Hippocampal volume does not change seasonally in a non food-storing songbird.

Seasonal differences in hippocampal morphology have been reported in food-storing birds. Non food-storing species have not been investigated however. It is therefore unclear whether seasonal changes in the hippocampus are specifically related to food-storing or reflect a more general seasonal mechanism that occurs in both food-storing and non food-storing birds alike. We determined the volumes of the hippocampal formation and remaining telencephalon in the non-storing male song sparrow (Melospiza melodies morphna) in two experiments comparing birds collected in the spring and fall of 1992-94 (Experiment 1) and 1997 (Experiment 2). Although pronounced seasonal changes in song control nuclei such as the HVC and RA were previously reported for the same brains used in Experiment 1, we found that hippocampal volume did not change with season in either Experiment 1 or 2 for these song sparrow brains. These results suggest that seasonal changes in the hippocampus do not occur in this non food-storing species and may be specific to food-storing birds.

Animals↗

Oestrogen regulates male aggression in the non-breeding season.

Extensive research has focused on territorial aggression during the breeding season and the roles of circulating testosterone (T) and its conversion to 17beta-oestradiol (E2) in the brain. However, many species also defend territories in the non-breeding season, when circulating T-levels are low. The endocrine control of non-breeding territoriality is poorly understood. The male song sparrow of Washington State is highly territorial year-round, but plasma T is basal in the non-breeding season (autumn and winter). Castration has no effect on aggression in autumn, suggesting that autumnal territoriality is independent of gonadal hormones. However, non-gonadal sex steroids may regulate winter territoriality (e.g. oestrogen synthesis by brain aromatase). In this field experiment, we treated wild non-breeding male song sparrows with a specific aromatase inhibitor (fadrozole, FAD) using micro-osmotic pumps. FAD greatly reduced several aggressive behaviours. The effects of FAD were reversed by E2 replacement. Treatment did not affect body condition or plasma corticosterone, suggesting that all subjects were healthy These data indicate that E2 regulates male aggression in the non-breeding season and challenge the common belief that aggression in the non-breeding season is independent of sex steroids. More generally, these results raise fundamental questions about how sexual and/or aggressive behaviours are maintained in a variety of model vertebrate species despite low circulating levels of sex steroids or despite castration. Such non-classical endocrine mechanisms may be common among vertebrates and play an important role in the regulation of behaviour.

Aggression↗

Global warming and body mass decline in Israeli passerine birds.

Global warming may affect the physiology, distributions, phenology and adaptations of plants and animals. In Israel, minimum summer temperatures increased by an average of 0.26 degrees C per decade during the second half of the 20th century. Bergmann's rule predicts that, in warm-blooded animals, races from warm regions are smaller than races from cold regions. Numerous studies have reported general correlations between body mass in fossil animals and independently established palaeoclimatic changes from various parts of the world in accordance with this rule. Using museum specimens, I tested the prediction that the body mass and tarsus length of five resident passerine species in Israel declined between 1950 and 1999. The body mass of four species (the graceful warbler Prinia gracilis, the house sparrow Passer domesticus, the yellow-vented bulbul Pycnonotus xanthopygos and the Sardinian warbler Sylvia melanocephala, but not of the crested lark Galerida cristata) declined significantly during this period. Tarsus length also declined significantly during this period for two species (the graceful warbler and the house sparrow). Body condition (body mass-to-tarsus length ratio) decreased in the Sardinian warbler, the yellow-vented bulbul and the crested lark. It is suggested that the above declines in body mass and tarsus length are due to global warming and also in accordance with Bergmann's rule. The above explanation does not exclude the possibility that other factors, such as a decrease in food availability, contributed to the decline in body mass. These declines may have serious implications for community structure and competition among bird species and may affect the survival of small passerines.

Acclimatization↗

Is song-type matching a conventional signal of aggressive intentions?

Song-type matching is a singing strategy found in some oscine songbirds with repertoires of song types and at least partial sharing of song types between males. Males reply to the song of a rival male by subsequently singing the same song type. For type matching to serve as an effective long-distance threat signal, it must be backed up by some probability of aggressive approach and impose some type of cost on senders that minimizes the temptation to bluff. Western subspecies of the song sparrow exhibit moderate levels of song-type sharing between adjacent males and sometimes type match in response to playback of song types they possess in their repertoires. Interactive playback experiments were used in order to examine the subsequent behaviour of type-matching birds and to quantify the responses of focal birds to type-matching versus non-matching stimuli. Birds that chose to type match the playback of a shared song type subsequently approached the speaker much more aggressively than birds that did not type match. Moreover, birds approached a type-matching stimulus much more aggressively than a non-matching stimulus. These results and consideration of alternatives suggest that type matching in song sparrows is a conventional signal in which honesty is maintained by a receiver retaliation cost against bluffers.

Animals↗

Inadvertent social information in breeding site selection of natal dispersing birds.

Several species use the number of young produced as public information (PI) to assess breeding site quality. PI is inaccessible for synchronously breeding birds because nests are empty by the time the young can collect this information. We investigate if location cues are the next best source of inadvertent social information (ISI) used by young prospectors during breeding site choice. We experimentally deployed ISI as decoys and song playbacks of breeding males in suitable and sub-optimal habitats during pre- and post-breeding periods, and monitored territory establishment during the subsequent breeding season for a social, bobolink (Dolichonyx oryzivorus), and a more solitary species, Nelson's sharp-tailed sparrow (Ammodramus nelsoni). The sparrows did not respond to treatments, but bobolinks responded strongly to post-breeding location cues, irrespective of habitat quality. The following year, 17/20 sub-optimal plots to which bobolink males were recruited were defended for at least two weeks, indicating that song heard the previous year could exert a "carry-over attraction" effect on conspecifics the following year. Sixteen recruited males were natal dispersers, as expected when animals have little opportunity to directly sample their natal habitat quality. We suggest that differences in breeding synchronicity may induce an equivalent clinal distribution of ISI use.

Animals↗

Complexity and integration in sexual ornamentation: an example with carotenoid and melanin plumage pigmentation.

Sexual ornaments often consist of several components produced by distinct developmental processes. The complexity of sexual ornaments might be favoured by mate choice of individual components in different environments which ultimately results in weak interrelationships (integration) among the developmental processes that produce these components. At the same time, sexual selection for greater exaggeration of individual components favours their stronger co-dependence on organismal resources. This should ultimately produce stronger condition-mediated integration among ornaments' components in individuals with the most exaggerated ornamentation. Here we distinguish between these two sources of integration by examining the relationship between integration and elaboration of sexual ornamentation in three bird species: two with carotenoid-based sexual ornamentation (the house finch, Carpodacus mexicanus and common redpoll, Carduelis flammea) and a species with melanin-based sexual ornamentation (house sparrow, Passer domesticus). We found that integration of components varied with elaboration of carotenoid-based ornamentation but not of melanin ornamentation. In the house finches, integration was the highest in individuals with small ornaments and decreased with ornament elaboration whereas the pattern was the opposite in common redpolls. These results suggest that in these species integration and complexity of carotenoid-based ornamental components are due to shared condition-dependence of distinct developmental pathways, whereas integration and complexity of the melanin ornamentation is due to organismal integration of developmental pathways and is largely condition- and environment-invariant. Thus, functionally, ornamentation of the house sparrows can be considered a single trait, whereas complexity of the house finch and redpoll ornamentation varies with ornament elaboration and individual condition.

Animals↗

Categorical perception of a natural stimulus continuum: birdsong.

A fundamental issue in perception and communication is how continuously varying stimuli are partitioned into discrete categories. In swamp sparrow songs, note duration is a critical feature distinguishing two note categories with different roles in song construction. Pairs of songs with initial notes from different categories contrast more in their effects on territorial males than song pairs with initial notes differing by the same amount but taken from within one note category. The results indicate categorical perception by wild swamp sparrows.

Animal Communication↗

Avian pneumovirus (APV) RNA from wild and sentinel birds in the United States has genetic homology with RNA from APV isolates from domestic turkeys.

Nasal turbinates or swabs were collected from wild ducks, geese, owls, sparrows, swallows, and starlings and from sentinel ducks placed next to turkey farms experiencing avian pneumovirus (APV) infections and were analyzed for APV genome and infectious particles. APV RNA was detected in samples examined from geese, sparrows, and starlings. APV RNA and antibodies were also detected in two different groups of sentinel ducks. Infectious APV was recovered from sentinel duck samples. The APV M gene isolated from the wild birds had over 96% predicted amino acid identity with APV/Minnesota 2A, which was isolated earlier from domestic turkeys showing respiratory illness, suggesting that wild birds may be involved in spreading APV infection.

Amino Acid Sequence↗

Detection of anti-West Nile virus immunoglobulin M in chicken serum by an enzyme-linked immunosorbent assay.

The emergence of West Nile (WN) virus in New York and the surrounding area in 1999 prompted an increase in surveillance measures throughout the United States, including the screening of sentinel chicken flocks for antibodies. An enzyme-linked immunosorbent assay (ELISA) for the detection of chicken immunoglobulin M (IgM) to WN virus was developed, standardized, and characterized as a rapid and sensitive means to detect WN viral antibodies in sentinel flocks. Serum specimens from experimentally infected chickens were analyzed by using this assay, and IgM was detected as early as 3 to 7 days postinfection. Persistence of IgM varied from at least 19 to more than 61 days postinfection, which indicates the need to bleed sentinel flocks at least every 2 weeks for optimal results if this method is to be used as a screening tool. The ELISA was compared to hemagglutination-inhibition and plaque reduction neutralization tests and was found to be the method of choice when early detection of WN antibody is required. House sparrows and rock doves are potential free-ranging sentinel species for WN virus, and the chicken WN IgM-capture ELISA was capable of detecting anti-WN IgM in house sparrow serum samples from laboratory-infected birds but not from rock dove serum samples. The chicken WN IgM-capture ELISA detected anti-WN antibodies in serum samples from naturally infected chickens. It also detected IgM in serum samples from two species of geese and from experimentally infected ring-necked pheasants, American crows, common grackles, and redwinged blackbirds. However, the test was determined to be less appropriate than an IgG (IgY)-based assay for use with free-ranging birds. The positive-to-negative ratios in the ELISA were similar regardless of the strain of WN viral antigen used, and only minimal cross-reactivity was observed between the WN and St. Louis encephalitis (SLE) IgM-capture ELISAs. A blind-coded serum panel was tested, and the chicken WN IgM-capture ELISA produced consistent results, with the exception of one borderline result. A preliminary test was done to assess the feasibility of a combined SLE and WN IgM-capture ELISA, and results were promising.

Animals↗

Night vision in the elderly: consequences for seeing through a "blue filtering" intraocular lens.

Relative scotopic spectral sensitivity depends only on the rhodopsin photopigment and ocular media absorption spectra. Rhodopsin is well characterised so the relative scotopic spectral sensitivity function can be calculated for intraocular lenses (IOLs) of known spectral density. In a recent perspective, Mainster and Sparrow concluded that an IOL with short wave absorbing chromophores would provide more retinal protection than conventional IOLs, but the practical consequences for scotopic vision are unclear. This paper uses published experiments to examine the implications for scotopic vision of the IOLs analysed by Mainster and Sparrow. A 14.6% reduction in scotopic sensitivity is expected for a SN60AT (AcrySof Natural) compared to a SA60AT (Conventional AcrySof) IOL under broadband illumination (equal quantum spectrum). This effect (0.07 log unit) is visually insignificant in relation to the approximately 4.0 log unit range of scotopic sensitivity. More importantly, it is expected that scotopic contrast sensitivity would be reduced by only approximately 0.01 log unit. It is thus improbable that a difference in scotopic vision between observers with the Natural and Conventional IOLs could be reliably detected using broadband stimuli.

Absorption↗

Evidence for a novel gonadotropin-releasing hormone in hypothalamic and forebrain areas in songbirds.

One or more of three distinct forms of gonadotropin-releasing hormones (GnRHs), has been found in all vertebrates studied. Birds are known to contain two types of GnRH, chicken GnRH-I and -II (cGnRH-I and -II), but to date nobody has investigated the possibility of the presence of lamprey GnRH-III in birds. Thus, our objective was to investigate the presence and distribution of immunoreactive lamprey (ir-lamprey) GnRH-III in hypothalamic and forebrain areas in house sparrows (Passer domesticus) and Gambel's white-crowned sparrows (Zonotrichia leucophrys gambelii). In addition to its distribution in the CNS, we investigated whether or not exogenous lamprey GnRH-III peptide could elicit luteinizing hormone (LH) release in vivo. This study presents two novel findings: 1) The widespread presence in the central nervous system of an immunoreactive third GnRH in birds that is clearly hypophysiotropic and has gonadotropin-releasing capabilities. Intravenous injection of 100 ng lamprey-GnRH-III peptide elicited a rapid rise in circulating luteinizing hormone as compared to controls. This third GnRH, ir-lamprey GnRH-III, likely has multiple functions, as suggested by its widespread distribution. 2) Ir-lamprey GnRH-III is present in abundance in telencephalic areas, including the hippocampal formation and the song control system. In no vertebrate has a GnRH been localized in these 'higher' control regions before. The fact that ir-lamprey-GnRH-III is present in auditory processing areas (higher vocal center, or HVc, area X, caudomedial neostriatum) and song-producing areas (HVc, robust nucleus of the neostriatum, or RA) suggests a direct way in which auditory processing and song production can affect circulating gonadotropins.

Animals↗

Different colors reveal different information: how nutritional stress affects the expression of melanin- and structurally based ornamental plumage.

Avian plumage colors have emerged recently as model systems for investigating the types of information that can be signaled by showy sexual displays in animals. In many species, the brightness of carotenoid-based plumage reflects the health and condition of individuals and is used in mate selection. The information contained in melanin-based and structurally based ornamental colors in birds is less well resolved, however. We subjected male house sparrows Passer domesticus and brown-headed cowbirds Molothrus ater to stressful nutritional conditions during molt to test the hypothesis that melanin- and structurally based plumage colors are nutritionally condition-dependent. We restricted food access for treatment males during randomized 6 h periods on 4 days per week, while allowing control birds access to food ad libitum throughout the course of the molt. We found that the size and brightness of the melanin-based throat badges in male house sparrows were not affected by nutritional stress. Similarly, there were no differences between treatment and control male cowbirds in the size or brightness of the melanin-based brown hood. However, the structurally based iridescent plumage of cowbirds was indicative of the nutritional condition of males during molt. Nutritionally stressed cowbirds grew significantly less colorful plumage than did males with access to food ad libitum. These results are consistent with observations in other avian species that different types of plumage color communicate different sets of information. Melanin ornaments are less sensitive to nutritional conditions during molt and instead may reflect the hormonal status and/or competitive ability of males, whereas structural coloration appears to be an accurate signal of health and condition.

Animal Nutritional Physiological Phenomena↗

Varied pathogenicity of a Hong Kong-origin H5N1 avian influenza virus in four passerine species and budgerigars.

This investigation assessed the ability of the zoonotic A/chicken/Hong Kong/220/97 (chicken/Hong Kong) (H5N1) highly pathogenic avian influenza virus to infect and cause disease in zebra finches (Taeniopygia guttata), house finches (Carpodacus mexicanus), house sparrows (Passer domesticus), European starlings (Sternus vulgaris), and budgerigars (Melopsittacus undulatus) after intranasal administration. Zebra finches were the most severely affected of the five species, demonstrating anorexia, depression, and 100% mortality within 5 days of inoculation. Gross lesions in this species were absent or only mild. But histologic lesions and the corresponding viral antigen were observed in multiple organs, especially in the nasal cavity, brain, pancreas, spleen, adrenal glands, and ovary. Significant morbidity and mortality also were observed in both house finches and budgerigars. Affected birds of these two species demonstrated anorexia, depression, and neurologic signs and typically were moribund or dead within 2 days of the onset of clinical signs. Gross lesions were mild or absent in house finches and budgerigars. Histologically, the brain and pancreas were the most consistently and severely affected organs in house finches. The brain was the most affected organ in budgerigars. Unlike these three species, house sparrows suffered only mild transient depression, had no mortality, and lacked gross lesions. Viral antigen and microscopic lesions were observed only in the heart and testicle of a minority of birds of this species. Starlings demonstrated neither clinical disease nor mortality and lacked gross and histologic lesions. Viral antigen was not observed in any of the collected tissues from starlings. These results indicate that there is significant variation in the pathogenicity of the chicken/Hong Kong virus for different species of birds, including species within the same order. In addition, neurotropism is a recurrent feature among birds that eventually succumb to infection.

Adrenal Glands↗

Reservoir competence of native North American birds for the lyme disease spirochete, Borrelia burgdorfieri.

Reservoir competence for the Lyme disease spirochete, Borrelia burgdorferi, was tested for six species of native North American birds: American robin, gray catbird, brown thrasher, eastern towhee, song sparrow, and northern cardinal. Wild birds collected by mist netting on Fire Island, NY, were held in a field laboratory in cages over water and locally collected larval ticks were placed on the birds, harvested from the water after engorgement, and tested for infection by direct fluorescentantibody staining after molting to the nymphal stage. American robins were competent reservoirs, infecting 16.1% of larvae applied to wild-caught birds, compared with 0% of control ticks placed on uninfected laboratory mice. Robins that were previously infected in the laboratory by nymphal feeding infected 81.8% of applied larvae. Wild-caught song sparrows infected 4.8% of applied larvae and 21.1% when infected by nymphal feeding. Results suggest moderate levels of reservoir competence for northern cardinals, lower levels for gray catbirds, and little evidence of reservoir competence for eastern towhees or brown thrashers. Lower infection rates in larvae applied to wild-caught birds compared with birds infected in the laboratory suggest that infected birds display temporal variability in infectiousness to larval ticks. Engorged larvae drop from birds abundantly during daylight, so the abundance of these bird species in the peridomestic environment suggests that they might contribute infected ticks to lawns and gardens.

Animals↗

Antibody response of wild birds to natural infection with alphaviruses.

From 1986 to 1990, we conducted our second longitudinal study in the central (upstate) New York (CNY) area on the wild avian hosts of eastern equine encephalomyelitis (EEE) virus. Field-collecting methods mirrored a study conducted from 1978 to 1980 at the same endemic focus. Over the 5-yr study period, we captured 6,296 birds representing 99 species and took 4,174 blood samples from representatives of 83 species. Gray catbirds, song sparrows, and veerys were the three dominant species captured and bled, accounting for 40 and 55% of birds captured and bled. Blood clots were assayed for virus and sera tested for hemagglutination-inhibition (HI) antibodies to EEE and Highlands J virus. Virus isolations from birds defined two epiornitics of EEE virus in 1988 and 1990, and an epiornitic of HJ virus in 1986. Infected birds responded with the production of HI antibodies with titers indicative of recent infection (HI > or = 1:160), and titers of sera positive during the epiornitics were significantly higher than positive sera during nonepiornitics. The 1990 EEE epiornitic extended from mid-July to the end of September, providing data to compare infection rates among species, habitats, and combinations of species with habitats. Few significant differences were found. The HJ epiornitic was only the second time this virus has occurred in CNY. Song sparrows were identified as the primary amplifying avian host of both viruses, although our capture and serological data would suggest a role for gray catbirds as the species most likely involved in yearly virus reintroduction. However, the cryptic nature of enzootic virus maintenance remains unresolved for the CNY virus foci. The appearances of HJ and EEE viruses were not epidemiologically linked, and there were no virus isolations from adults returning on site or virus isolations without concurrent isolations from mosquito vectors. Whether EEE and/or HJ virus are consistently present in or sporadically introduced into the inland foci of CNY area still has not been determined.

Alphavirus↗

Plasma binding proteins as mediators of corticosteroid action in vertebrates.

Stressors elicit a complex but variable suite of endocrine events. Comparative studies of the stress response have focused primarily on the adrenocortical response to stress, in particular the measurement of plasma levels of glucocorticoids. However, a number of other factors contribute to and modify cellular and organismal responses to glucocorticoids. Notably, plasma corticosteroid binding globulins (CBGs) can regulate the general availability of steroid to tissues, and/or direct the delivery of hormones to specific sites. In this paper, we discuss possible functions of CBG and mechanisms of CBG action, review CBG characteristics among vertebrates, and discuss our recent studies indicating that CBG may indeed modulate responses to stressors. For example, in house sparrows, we found that basal and stress-induced concentrations of total corticosteroid (cortisol or corticosterone) (CORT) vary seasonally, but CBG concentrations change proportionally, so that free CORT concentrations appear static year-round. In contrast, in white-crowned sparrows and tree lizards, CBG concentrations change under conditions when total CORT levels do not, resulting in significant changes in circulating free CORT. These differences in free CORT are masked if CBG is not accounted for. We have also found that the binding properties of CBG vary considerably between species and need to be determined empirically. Such studies led to the observation that CBG in several species may also serve as a functional androgen binding protein; this is especially important for birds, because previous studies had concluded that birds lack androgen binding globulins. We propose that consideration of CBG is paramount to understanding the role of glucocorticoids in mediating behavioral and physiological responses to stress.

Adrenal Cortex Hormones↗

Proximate and landscape factors influence grassland bird distributions.

Ecologists increasingly recognize that birds can respond to features well beyond their normal areas of activity, but little is known about the relative importance of landscapes and proximate factors or about the scales of landscapes that influence bird distributions. We examined the influences of tree cover at both proximate and landscape scales on grassland birds, a group of birds of high conservation concern, in the Sheyenne National Grassland in North Dakota, USA. The Grassland contains a diverse array of grassland and woodland habitats. We surveyed breeding birds on 2015 100 m long transect segments during 2002 and 2003. We modeled the occurrence of 19 species in relation to habitat features (percentages of grassland, woodland, shrubland, and wetland) within each 100-m segment and to tree cover within 200-1600 m of the segment. We used information-theoretic statistical methods to compare models and variables. At the proximate scales, tree cover was the most important variable, having negative influences on 13 species and positive influences on two species. In a comparison of multiple scales, models with only proximate variables were adequate for some species, but models combining proximate with landscape information were best for 17 of 19 species. Landscape-only models were rarely competitive. Combined models at the largest scales (800-1600 m) were best for 12 of 19 species. Seven species had best models including 1600-m landscapes plus proximate factors in at least one year. These were Wilson's Phalarope (Phalaropus tricolor), Sedge Wren (Cistothorus platensis), Field Sparrow (Spizella pusilla), Grasshopper Sparrow (Ammodramus savannarum), Bobolink (Dolychonix oryzivorus), Red-winged Blackbird (Agelaius phoeniceus), and Brown-headed Cowbird (Molothrus ater). These seven are small-bodied species; thus larger-bodied species do not necessarily respond most to the largest landscapes. Our findings suggest that birds respond to habitat features at a variety of scales. Models with only landscape-scale tree cover were rarely competitive, indicating that broad-scale modeling alone, such as that based solely on remotely sensed data, is likely to be inadequate in explaining species distributions.

Animals↗

Advancing schedules and constant light produce faster resynchronization of circadian rhythms.

House sparrows, Passer domesticus, were subjected to rotated light-dark (LD) cycles that consisted of repeated 8-hr advances or delays of 5 days of LD 8:16, with intervening 40-80 hr of constant dark or light. Sparrows reset the fastest (by the second cycle) when they were advanced with intervening constant light (LL). They reset the slowest (taking six cycles) when they were delayed with intervening constant dark (DD).

Animals↗