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Mycobacterium bovis infection in wildlife in New Zealand.

Bovine tuberculosis (Tb) is the most important disease of livestock in New Zealand, and it puts at risk the nation's trade in dairy, beef and venison products. Elimination of the disease from livestock is based on a herd test and slaughter programme and carcass inspection at abbatoirs. However, this programme has not been as successful as expected, because the disease also occurs in wild or feral animals acting as vectors of the disease to livestock. Brushtail possums are the major wildlife vector and self-sustaining maintenance host of Tb, and play a role analogous to that of the badger in Great Britain. In contrast, some deer species and ferrets may act as vectors of the disease, but their role in transmitting Tb to livestock is unclear. Hedgehogs, pigs, cats, sheep and goats are now considered to be amplifier hosts, and spread the disease to other species only when inspected or their carcasses scavenged. In the absence of infected possum populations, these species do not appear to be capable of maintaining the infection in their own populations and are not thought to be involved in the maintenance of Tb in livestock. Tuberculosis has also been recorded from stoats, hares, and a rabbit, but the level of infection recorded in their populations indicates these species are unlikely to spread the disease to other animals and hence are not involved in the transmission of Tb to livestock.

Animals↗

Biotin studies in pigs. 1. Biotin deficiency in the young pig.

Sixteen pigs were given a purified diet of maize flour and casein from 5 to 25 d of age with or without a biotin supplement (100 micrograms/kg). From 26 to 94 d of age, eight of the pigs were changed to a wheat and casein diet with or without the biotin supplement and eight continued on the maize flour and casein diet. The weight gain and feed conversion ratio of the pigs were not affected by the dietary biotin supplement or the carbohydrate source. The pigs given the unsupplemented maize flour diet developed foot lesions, scaly skins and showed a reduction in carcass length. The excretion of biotin in the faeces was similar for the pigs given the biotin-supplemented and unsupplemented diets. The excretion of biotin in the faeces of pigs given the wheat diet was ten times as great as that of the pigs given the maize flour diet. The excretion of biotin in urine was 6 micrograms/d in the pigs given the unsupplemented maize flour diet and 67 micrograms/d in the pigs given the biotin-supplemented diet between 91 and 94 d of age. The biotin contents of the liver, heart, kidney, adrenals and plasma were increased by biotin supplementation of the maize flour diet. The 16:1/16:0 and 18:1/18:0 fatty acids ratios in the liver were decreased by biotin supplementation of the maize flour diet.

Aging↗

Diagnostic methods in infectious respiratory disease.

For laboratory diagnosis of respiratory disease it is of overwhelming importance that the specimens taken are adequate, taken from the correct site and at the correct time. The lower regions of the respiratory tract are particularly difficult to sample but are more likely to yield the causative agent of a pneumonia. Infections involving the upper respiratory tract are much easier to sample and appropriate aspiration apparatus can be used. Consideration must be given to the timing of sample collection in relation to the life cycle of the causative micro-organism. Sampling of several animals is recommended. Diagnosis may be achieved by isolation in culture of the causative agent or the demonstration of the agent by indirect methods such as electron microscopy and ELISA. Clinical biochemical tests may reflect systemic metabolic changes induced by microbial infections and give an indication of the severity of the disease and its prognosis. Pulmonary function tests have limited application in animals and are only likely to be used under experimental conditions and in horses and small animals.

Animals↗

Serological diagnosis of Erysipelothrix rhusiopathiae: a comparative study between the growth inhibition test and the complement fixation test.

A Complement Fixation Test (CFT) was developed for the diagnosis of Erysipelas infections. The results of that work were compared with those of the Growth Inhibition Test (GIT), and they showed that the CFT test was very useful to determine whether an animal had been vaccinated or was still suffering from the disease. The CFT is as reliable as the GIT in detecting recent as well as chronic infections, but its value is limited where animals are examined for their response to a vaccination. The test is quick, easy, and safe to conduct.

Animals↗

Lungworm and stomach worm infection in relation to body fat reserves and blood composition in wild boar (Sus scrofa).

Lung and stomach nematode burden was estimated in 8 juvenile and 12 adult wild boar (Sus scrofa), shot in The Royal Forestry 'Het Loo' between January 1988 and April 1989, in order to study whether body fat reserves or blood composition (i.e., nutritional status) is correlated with the parasite burden. Only bone marrow fat, the last fat reserve to be mobilized, showed a slight negative correlation with the stomach worm burden. Serum urea concentration, an indicator of severe undernutrition, was weakly correlated with the stomach worm burden in juvenile boars. These results suggest a relatively small impact of these parasites on animals with a lowered nutritional status. The burden of lungworms or stomach worms did not provide reliable information about the body condition of wild boar.

Adipose Tissue↗