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Effects of chromatic adaptation on phase-dependent sensitivity to heterochromatic flicker.

Temporal modulation sensitivity was measured as a function of the relative phase of two equiluminous chromatic sources (564 and 625 nm) for temporal frequencies from 6 to 20 Hz. The difference between 180 degrees and the phase of least sensitivity was computed as the measured phase shift. A 2 degree test field was superimposed upon 8 degrees chromatic adapting fields with luminances from 100 to 3000 Td and chromaticities of 500, 600, and 650 nm. For each adapting field, the 564-nm source was set to 175 Td, and the 625-nm source was matched to it with heterochromatic flicker photometry (giving an effective mean luminance of 350 Td). The 650-nm adapting fields produced large changes in photometric setting but only small changes in the measured phase shift. The 600- and 500-nm adapting fields produced smaller changes in photometric setting but larger changes in the measured phase shift. In general, increased adapting luminance resulted in an increase in the measured phase shift for 600-nm adaptation and a decrease in the measured phase shift for 500-nm adaptation.

Adaptation, Physiological↗

Black spectral responsivity.

Six subjects induced blackness within a circular broadband field by increasing the radiance of a surrounding monochromomatic annulus, which varied in wavelength. Between the central field and the annulus was a thin dark ring. Half of the subjects were instructed to increase the radiance of the annulus until the central field just turned black, and the other half were instructed to increase the radiance of the annulus until the contour between the central field and the dark ring disappeared. Spectral luminous efficiency functions measured by the methods of heterochromatic flicker photometry (HFP) and brightness matching (HBM) were determined for each subject and compared with the subject's blackness-induction functions. The hypothesis that the contour-disappearance instruction would yield blackness-induction curves best fitted by flicker photometric functions and that the absolute-blackness instruction would yield blackness-induction curves best fitted by HBM functions was not confirmed. There was only one subject for whom the spectral efficiency of blackness was represented better by HFP than by HBM. There was one subject for whom blackness spectral efficiency was fitted better by HBM than by HFP. For the remaining four subjects, there was no difference in fits.

Color Perception↗

[Experiments aimed at developing an implantable and continuously functioning glucose sensors based on polarimetry].

In vitro and in vivo experiments devised with the aim of developing a permanently implantable glucose sensor based on polarimetry are described. It was found that in ultrafiltrated human blood plasma the overall optical rotation was 94% specific for glucose, and that polarisation photometry yielded a sufficiently sensitive signal for in vivo glucose detection. The three types of capillary membrane intended for implantation that we tested, revealed an in vitro response time to glucose concentration of 10 minutes; when implanted, they maintain this over a period of weeks, during which time the same glucose concentrations can be measured daily in ultrafiltrated capillary fluid as in the blood of the animals (guinea pigs). The drop in glucose concentration induced 20 minutes after a single administration of insulin can also be detected in both fluids. The experiments described indicate that the development of an implantable polarimetric glucose sensor is possible.

Animals↗

Intermethod variability of sodium and potassium results: patients' sera and commercially available control sera.

Sodium and potassium were measured in sets of 102 to 107 patients sera, and in 31 commercially available control sera. The results from four routine analytical methods/systems (indirect potentiometry: two; direct potentiometry and enzymatic assay: one each) were compared with those from a flame photometry-based reference method. In the assay of patient sera, substantial agreement was observed in some comparisons, clinically relevant bias in others. The inter-assay changes observed for the control sera differed significantly from those shown by the patients sera (i.e. commercial control sera were non-commutable) in about 12% of the comparisons, as a whole. Recalculation of serum sample results with a single control serum as calibrator lowered or increased the bias originally present according to whether the serum itself was commutable or not. Moreover, the inter-method variability in the assay of commercial control sera was lower with commutable sera, higher with non-commutable sera. With the exception of liquid sera stabilized with ethylene glycol, there was no evident link between any specific characteristic of the commercial control sera (matrix and physical state) and their degree of commutability.

Animals↗

Length of sedimentation reaction in blood: a comparison of the test 1 ESR system with the ICSH reference method and the sedisystem 15.

The aim of this study was to compare the performance of the automatic TEST 1 ESR system, SIRE Analytical Systems (TEST 1), with that of the the Sedisystem 15, Becton Dickinson (SEDI), and the International Council for Standardization in Haematology reference method (Westergren) for measuring the length of sedimentation reaction in blood (LSRB). This reaction was measured in 418 paired blood samples drawn in K2-EDTA vacuum tubes and specific tubes from patients scheduled for routine LSRB measurement. The TEST 1 system uses micro-sedimentation and quantitative capillary photometry technology, whereas the SEDI uses a CCD camera. For Westergren, a 200 mm column with 3.0 mm internal diameter was used. Compared to Westergren, TEST 1 gives accurate values of LSRB in most of the samples (mean of differences: 0.99 +/- 10.4 mm; 95% CI, -0.807 to 2.78 mm; n =131). Similar results were obtained in the comparison with SEDI (mean of differences: -0.626 +/- 8 mm; 95% CI, -1.756 to 0.5 mm; n = 195). Compared to those of fresh blood samples, LSRB values were significantly lower in 24 h stored samples, either at 4 degrees C (21.5 +/- 2.3 vs. 19.4 +/- 2.2 mm; p (Spearman's coefficient of correlation): 0.981; n = 44) or at room temperature (19.1 +/- 2.5 vs. 16.2 +/- 2.1 mm; p: 0.903; n = 46). In conclusion, TEST 1 is a rapid, reliable system for automatic measurement of LSRB in standard K2-EDTA blood samples. It has a very low imprecision and maintains a good performance in 24 h stored samples. In addition, due to its operational characteristics (60 samples/20 min) it is a suitable tool for clinical laboratories with a high work load as well as for emergency laboratories.

Adolescent↗

Recommendation for measuring and reporting chloride by ISEs in undiluted serum, plasma or blood.

The proposed recommendation for measuring and reporting chloride in undiluted plasma or blood by ion-selective electrodes (ISEs) will provide results that are identical to chloride concentrations measured by coulometry for standardized normal plasma or blood samples. It is applicable to all current ISEs dedicated to chloride measurement in undiluted samples that meet the requirements. However, in samples with reduced water concentration, results by coulometry are lower than by ion-selective electrode due to volume displacement. The quantity measured by this standardized ISE procedure is called the ionized chloride concentration. It may be clinically more relevant than the chloride concentration as determined by coulometry, photometry or by ISE after dilution of the sample.

Analysis of Variance↗

Relationship between plasma growth hormone concentration and cellular sodium transport in acromegaly.

We investigated the relationship between mean plasma growth hormone (GH) concentration and cellular sodium transport in untreated and treated acromegaly. Seventeen patients (age 55 +/- 3 years) with active acromegaly were studied with respect to plasma GH (mean of 24 h GH profile) and erythrocyte electrolyte content as well as transmembrane sodium transport. The patients were reinvestigated two weeks after successful surgery (N = 14) and again after one year (N = 13). Erythrocyte electrolytes were analyzed by flame photometry and sodium influx and efflux rate constant determined by in vitro incubation using a modified Keyne's formula. In patients with active acromegaly there was a significant positive correlation between IGF-1 and cellular sodium transport, while GH tended to show a negative relationship to the same parameter. After successful treatment, both IGF-1 and GH disclosed a positive relationship to cellular sodium transport. After one year, a significant increase in erythrocyte sodium content was seen in the patients compared to the preoperative situation. In conclusion, if this is a generalized phenomenon the results are compatible with a sodium-retaining effect of GH via stimulation of transmembrane sodium transport. In active acromegaly this may be counteracted by a sodium transport inhibitor giving the reverse relationship between GH and cellular sodium transport.

Acromegaly↗

Falsely high urinary catecholamines induced by labetalol.

A preoperative patient with pheochromocytoma was satisfactorily treated with oral labetalol, while a conspicuous increase in urinary output of catecholamines (CA) and of vanillylmandelic acid (VMA) was observed after labetalol therapy. Exaggerated increases in urinary CA and in VMA were also confirmed in normal volunteers immediately after oral labetalol. These effects of labetalol on urinary CA and VMA, however, were proved to be largely due to the interference with the usual photometries rather than due to its stimulation of CA release. Therefore, it should be emphasized that clinical evaluations of CA and its metabolites must be performed before labetalol therapy to avoid an erroneous diagnosis of pheochromocytoma.

Adrenal Gland Neoplasms↗

Criteria to evaluate bone mineralization in cattle: II. Noninvasive techniques.

An experiment was conducted to evaluate the capabilities of dual photon absorptiometry (PA), radiographic photometry (RP), and ultrasound (U) to estimate bone mineral content (BMC) and bone strength of a group of bovine third metacarpals (McIII). Metacarpals were chosen for evaluating BMC and bone strength because of their accessibility and susceptibility to biomechanical stress. The right and left McIII of 14 Angus heifers (24 to 32 mo of age) were collected at slaughter and all soft tissues (including periosteum) were removed. The BMC was estimated at both the midpoint and 3 cm proximal to the midpoint on the McIII diaphysis. Metacarpals then were tested by three-point bending to determine breaking load (BL) and breaking strength (BS). Bones were reassembled and two 2-cm sections were removed, one at the midpoint and one 1 cm proximal to the midpoint section. Sections then were ashed and ash content was expressed as grams per 2-cm slice and defined as BMC. Correlation coefficients (r) between BMC vs PA, RP, and U were .908 (P less than .0001), .967 (P less than .0001), and .565 (P less than .0001), respectively; r values between BS vs PA, RP, and U were .406 (P less than .05), .429 (P less than .05), and .499 (P less than .01), respectively, and r values between BL vs PA, RP, and U were .870 (P less than .0001), .865 (P less than .0001), and .588 (P less than .001), respectively. These data indicate that noninvasive techniques are useful in predicting BMC and BL in the bovine.

Absorptiometry, Photon↗

Prolactin and amniotic fluid electrolytes.

Prolactin (PRL) levels and Na+, K+, Cl-, Ca++ concentrations in maternal serum and amniotic fluid from 64 women in normal term pregnancy were measured by immunoenzymetric assay and flame photometry. The mean amniotic fluid PRL concentration was 597.7 (SE 31.5) ng/ml and the mean amniotic fluid Na+, K+, Cl- and Ca++ levels were 125.6 (SE 0.9) mmol/l, 4.5 (SE 0.1) mmol/l, 109.3 (SE 1.3) mmol/l and 2.0 (SE 7.5 E-02) mmol/l, respectively. There was no correlation between PRL levels in maternal serum and amniotic fluid, and the electrolyte concentrations in amniotic fluid. A close correlation was found between the concentrations of Na+ and Cl- in maternal serum and amniotic fluid. Thus, even though PRL may participate in the regulation of electrolytes in the amniotic fluid compartments, our findings provide indirect evidence for the existence of other regulatory mechanisms.

Amniotic Fluid↗

[Kinetics of corneal fluorescence in experimental keratitis].

It was shown by the method of scanning photometry suggested by the authors that rabbits with experimentally-induced keratitis displayed a three-phasic reaction of the area of the corneal burn: that of the enhanced fluorescein sorption at the reactive phase: that of the loss of the sorption capacity in the dystrophic stage, and that of the secondary absorption intensification during the regenerative stage. The mentioned phasic changes can be used for the diagnosis and objective assessment of the clinical course of keratitis.

Animals↗

Laser flare-cell meter analysis of blood-aqueous barrier functional status in a patient with crystalline retinopathy.

This is the first report in the literature of the application of a laser flare-cell meter to examine the functional status of the blood-aqueous barrier in a patient with crystalline retinopathy. The patient, a 34-year-old man with typical crystalline retinopathy, had crystal deposits scattered throughout the posterior pole of the fundus in both eyes. Fluorescein angiography showed atrophy of the retinal pigment epithelium and geographic areas of choriocapillaris atrophy in the posterior pole. Electroretinography showed subnormal amplitude, and the light-peak/dark-trough ratio of the electrooculogram was reduced. Laser photometry showed an increase in the aqueous flare intensity in both eyes, compared with normal values obtained from healthy control subjects. The increase in the aqueous flare intensity in this patient indicates that the function of the blood-aqueous barrier might have been affected in crystalline retinopathy.

Adult↗

Macular pigment optical density measurement: a novel compact instrument.

A compact device to derive the optical density of human macular pigment (MP) using heterochromatic flicker photometry is described. The validity of the system is assessed by measuring the optical density spectra of MP in 12 healthy subjects and comparing this with well-established previously published values. The mean spectral absorbance characteristics of MP across subjects corresponds well with accepted values. As reported in other studies, our measurements show a wide variation of MP optical densities between individuals. In our technique within-subject variability is low; standard deviations are between 0.025 and 0.15 in most cases. The overall optical density of MP ranged from 0.08 to 0.84 with a mean of 0.496 and standard deviation of 0.257 at 460 nm. The stimulus size was 0.95 degrees. The unique feature of the technique is that it allows free viewing (not Maxwellian View) of the stimulus, it can be conducted easily and quickly and does not need frequent re-calibration.

Humans↗

Macular pigment optical density before and after cataract extraction.

PURPOSE: Psychophysical methods of measuring macular pigment (MP) use comparisons of short- and midwave light in the fovea and parafovea to derive optical density estimates. This light must pass through the crystalline lens before absorption by the MPs can occur. The effect of lens absorption on these measures has not been adequately determined. The present study assesses the influence of lens absorption on MP measurements by comparing MP optical density (MPOD) measured before and after cataract extraction. METHODS: MPOD was measured using flicker photometry in free view at 458 nm with a 1 degrees stimulus. Twenty-nine eyes from 24 patients with cataracts sufficiently severe to require cataract extraction were evaluated. RESULTS: In the entire group of 24 patients, the mean (+/-SD) age measured 68.7 +/- 9.5 years, and the mean MPOD measured 0.19 +/- 0.11. For all 29 eyes measured, MPOD averaged 0.206 +/- 0.13 before and 0.18 +/- 0.12 after cataract extraction. MPOD measurements at the two time points (mean 8.1 +/- 4.7 weeks after surgery) were highly correlated (r = +0.58), suggesting that a cataractous lens does not influence the MP measurement technique. CONCLUSIONS: Psychophysical techniques can be used to obtain reliable measurements of MP in elderly subjects, even in those with cataracts. Moreover, differences in retinal illuminance due to varying opaqueness of the crystalline lens do not seem to have a measurable influence on MPOD.

Aged↗

Macular pigment and lutein supplementation in retinitis pigmentosa and Usher syndrome.

PURPOSE: To determine macular pigment (MP) in patients with inherited retinal degeneration and the response of MP and vision to supplementation of lutein. METHODS: Patients with retinitis pigmentosa (RP) or Usher syndrome and normal subjects had MP optical density profiles measured with heterochromatic flicker photometry. Serum carotenoids, visual acuity, foveal sensitivity, and retinal thickness (by optical coherence tomography [OCT]) were quantified. The effects on MP and central vision of 6 months of lutein supplementation at 20 mg/d were determined. RESULTS: MP density in the patients as a group did not differ from normal. Among patients with lower MP, there was a higher percentage of females, smokers, and light-colored irides. Disease expression tended to be more severe in patients with lower MP. Inner retinal thickness by OCT correlated positively with MP density in the patients. After supplementation, all participants showed an increase in serum lutein. Only approximately half the patients showed a statistically significant increase in MP. Retinal nonresponders had slightly greater disease severity but were otherwise not distinguishable from responders. Central vision was unchanged after supplementation. CONCLUSIONS: Factors previously associated with lower or higher MP density in normal subjects showed similar associations in RP and Usher syndrome. In addition, MP in patients may be affected by stage of retinal disease, especially that leading to abnormal foveal architecture. MP could be augmented by supplemental lutein in many but not all patients. There was no change in central vision after 6 months of lutein supplementation, but long-term influences on the natural history of these retinal degenerations require further study.

Adolescent↗

[Reversible erythrocyte aggregation in patients with schizophrenia].

Reversible aggregation of erythrocytes was studied in 33 male patients with paranoid schizophrenia aged 23-48 years. Minimum and maximum solidity of erythrocyte aggregates, spontaneous aggregation half-period, amplitude of photometric signal, aggregation index and integral coefficient were evaluated by photometry. A significant (p < 0.001) increase of reverse aggregation of erythrocytes closely correlated with phases of schizophrenic process.

Adult↗

Standardized detection of hepatitis B surface antigen: determination of its serum concentration in weight units per volume.

The concentration of HBsAg in serum was determined in arbitrary units by quantitative immunoelectrophoresis and in units of optical density by UV-photometry of purified antigen. The HBsAg was purified from serum by gelchromatography and subsequently by isopycnic centrifugation in cesium chloride. The ratio of the immunoelectrophoretically measured concentration to the units of optical density was found to be constant in six different samples with both subtypes - ad and ay - and varying concentration. The concentration of protein in three purified HBsAg samples was determined after acid hydrolysis by automatic aminoacid analysis. The specific extinction was E1 280 mg/ml = 4,5 +/- 0,3. Thus the arbitrary units of immunoelectrophoresis were converted to concentration of HBsAg specific protein, expressed as mug/ml. In 540 Ausria positive serum samples, mainly from the beginning of an acute hepatitis, 10-40 mug/ml was the most frequent range of concentration. About 4% of the sera contained more than 100 mug/ml, about 20% contained between 0,005 and 0,5 mug/ml and were positive only in RIA. 10 mug/ml correlated with titers in complement fixation of 1:32/64, in counterimmunoelectrophoresis of 1:8 and immuno-diffusion of 1:2. The use of standards with a defined concentration of HBsAg would allow a better control of the sensitivity in qualitative tests and of reproducibility in quantitation.

Animals↗

[Preparation, analysis and anti-anemic action of peroral powders with ferrous oxalate. Ferosol. 2].

Experiments with piglets showed good assimilation and metabolism of iron (II) in antianaemic powder Ferosol-1. In order to improve this drug, manganese, calcium (Ferosol-2, 1 composition) and ascorbic acid (Ferosol-2, 2 composition) were added. Manganese and ascorbic acid stimulate erythropoiesis and being strong reductants stabilize iron (II) on storage and in gastrointestinal tract. Components of Ferosol-2 were identified using colour, precipitation- and extraction tests, as well as paper chromatography and absorption spectroscopy. Quantitative analysis of microelements and ascorbic acid was performed using absorption spectroscopy, extraction photometry and iodometric titration methods. Ferosol-2 proved to be more stable than Ferosol-1. Lichfield and Wilkinson method modificated by Roth was used for detection of acute toxicity of Ferosol-2. A local action was estimated on quine pigs. Iron resorption was on 20 days old piglets. Antianaemic effectiveness of Ferosol-2 was evaluated on newborn piglets watching their weight. Experiments with white mice showed low toxicity of Ferosol-2 (LD50 = 4.3 g/kg, for 1 composition and 4.6 g/kg for 2 composition). It does not irritate mucosa of duodenum and ventriculus. Iron in Ferosol-2 was found to be well assimilated in gastrointestinal tract of piglets, easily gets into blood and joins albumen of plasma. After administration of Ferosol-2 to piglets increased iron concentration in blood serum was observed for 3 hours (1 composition) and 6 hours (2 composition). Experimental data showed increased resorption of iron in Ferosol-2, 2 composition due to ascorbic acid. Components of Ferosol-2 are well assimilated and join erythropoetic processes. Ferosol-2 leads to quicker weight gain as well as significantly higher amount of hemoglobin and red blood cells in piglets.

Administration, Oral↗