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At least 739 records · Page 41Linked to original sources
PubMed1987

Mapping of early development of electrical activity in the embryonic chick heart using multiple-site optical recording.

1. Using a multiple-site optical recording method with a 100-element photodiode array and a voltage-sensitive merocyanine-rhodanine dye, we have been able to monitor, for the first time, spontaneous electrical activity in pre-fused cardiac primordia in the 6- and 7-somite chick embryos. 2. To study the regional development of spontaneous electrical activity in the early embryonic pre-contractile chick heart at the later 7-9-somite stages, we have also recorded optically action potentials simultaneously from the entire heart, and constructed maps of the early development of electrical activity. 3. The data show that during the 6-9-somite stages, the size of the active area gradually increases, and that the development of electrical activity was spatially non-uniform: two peaks of activity were found in the right and left sides of the cono-ventricular region at the 7-8-somite stages. As development proceeded to the 9-somite stage, several peak areas of activity appeared. 4. The results are discussed in relation to the spatial pattern of proliferation of electrically active cells in the early phases of cardiogenesis.

Action Potentials↗
PubMed2000

Activity-dependent regulation of substance P expression and topographic map maintenance by a cholinergic pathway.

We have assessed the role of activity in the adult frog visual system in modulating two aspects of neuronal plasticity: neurotransmitter expression and topographic map maintenance. Chronic treatment of one tectal lobe with the non-NMDA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione decreased the percentage of substance P-like immunoreactive (SP-IR) tectal cells in the untreated lobe while disrupting topographic map formation in the treated one. Treatment with the NMDA receptor antagonist d-(-)-2-amino-5-phosphonovaleric acid (d-AP-5) disrupted the topographic map but had no affect on SP-IR cells. These results indicate that maintenance of the topographic map is dependent on direct input from the glutamatergic retinal ganglion cells, whereas substance P (SP) expression is being regulated by a pathway that relays activity from one tectal lobe to the other. Such a pathway is provided by the cholinergic nucleus isthmi, which is reciprocally connected to the ipsilateral tectum and sends a projection to the contralateral one. Mecamylamine and atropine, antagonists of nicotinic and muscarinic receptors, respectively, were used together to block all cholinergic activity or alone to block receptor subclass activity. All three treatments decreased SP expression and disrupted the topographic map in the treated tectal lobe. We conclude that both SP expression and topographic map maintenance in the adult optic tectum are activity-dependent processes. Although our results are consistent with the maintenance of the topographic map through an NMDA receptor-based mechanism, they suggest that SP expression is regulated by a cholinergic interaction that depends on retinal ganglion cell input only for its activation.

2-Amino-5-phosphonovalerate↗
PubMed2003

Orientation mosaic in barn owl's visual Wulst revealed by optical imaging: comparison with cat and monkey striate and extra-striate areas.

Using the technique of intrinsic signal optical imaging, orientation preference maps were obtained from the Wulst of the barn owl in the area that represents central vision, and from the visual cortices (V1 and V2) of cat and marmoset monkey. Iso-orientation domains in barn owl's visual Wulst were patch-like structures with an inter-patch distance of approximately 0.9 mm, arranged in a pinwheel-like manner around singularity points. The size of the iso-orientation domains in barn owl was larger than those found in area V1, but comparable to those found in area V2, of cat and monkey. Superficial layers of the owl visual Wulst may be equivalent to extra-striate visual areas of primates and carnivores, as already suggested by electrophysiologists discussing the much increased radial dimensions of the Wulst compared with neocortex in mammals.

Animals↗
PubMed2005

Simultaneous optical imaging of membrane potential and intracellular calcium.

Imaging of membrane potential (Vm) and intracellular calcium (Cai2+) is important for studying mechanisms of cardiac excitation, arrhythmias and defibrillation. We have developed an optical technique for simultaneous mapping of Vm and Cai2+ in cultured cell monolayers using fluorescent Vm- and Ca2+-sensitive dyes. Cultures of neonatal rat myocytes were double-stained with dyes RH-237 (Vm) and an analog of Fluo-3 or Rhod-2 dyes (Ca2+). These dyes have overlapping excitation spectra, allowing simultaneous excitation at the same wavelength range, and separate emission spectra allowing division of the fluorescent light into components sensitive to Vm and Cai2+, which were measured with two 16x16 photodiode arrays. It was found that Cai2+ measurements were strongly dependent on the properties of Ca2+-sensitive dyes. Thus, high-affinity Ca2+ dyes such as Fluo-4 and Rhod-2 reported Cai2+ transients approximately twice as long as those reported by low-affinity dyes Fluo-4FF and Rhod-FF. In addition, dyes with different affinities resulted in different measurements of Cai2+ responses to electrical shocks. When shocks were applied during the early plateau phase of the action potential, low-affinity dyes reported transient Cai2+ decreases at sites of both negative and positive Vm changes. In contrast, high-affinity Ca2+ dyes reported only a negligible change of plateau Cai2+ and a large elevation of diastolic Cai2+. These discrepancies between high- and low-affinity dyes were explained by a model of dye-ion interaction, which indicated that apparently long Cai2+ transients and Cai2+ responses to electrical shocks measured with high-affinity dyes were due to their non-linear response. These results indicate that optical measurements of Cai2+ transient duration and shock-induced Cai2+ changes require the use of low-affinity Cai2+ dyes.

Affinity Labels↗
PubMed1997

Development of orientation preference maps in area 18 of kitten visual cortex.

We investigated the development of orientation preference maps in the visual cortex of kittens by repeated optical imaging from the same animal. Orientation maps became detectable for the first time around postnatal day (P) 17 and improved continuously in strength unitl P30, the time at which their appearance became adultlike. During this developmental period the overall geometry of the maps remained unchanged, suggesting that the layout of the orientation map is specified prior to P17. Hence, before the visual cortex becomes susceptible to experience-dependent modifications its functional architecture is largely specified. This suggests that the initial development and layout of orientation preference maps are determined by intrinsic processes that are independent of visual experience. This conclusion is further supported by the result that orientation maps were well expressed at P24 in binocularly deprived kittens. Because the appearance of the first orientation-selective neurons and the subsequent development of orientation preference maps correlated well with the time course of the expression and refinement of clustered horizontal connections, we propose that these connections might contribute to the specification of orientation preference maps.

Animals↗
PubMed2006

Sluggish light for radio-frequency true-time-delay applications with a large time-bandwidth product.

A new radio-frequency (RF) photonic technique for achieving large RF time delays has been experimentally demonstrated using femtosecond pulses modulated by an acousto-optic tunable filter in a frequency-mapped and Doppler-shifted modulation scheme. A short optical delay line with length of 240 mum produces nearly 3 micros RF time delay after optical heterodyne detection, resulting in an effective slow-light velocity of 86 m/s. A delay-to-pulse-width ratio of 20 based on this technique has been observed, with a larger fractional delay foreseeable.

Journal Article↗
PubMedDate not supplied

Noninvasive imaging of oral premalignancy and malignancy.

Early detection of cancer and its precursors remains the best way to ensure patient survival and quality of life. Our specific aim is to test a multimodality approach to noninvasive diagnostics of oral premalignancy and malignancy. In the hamster cheek pouch model (120 hamsters), in vivo optical coherence tomography (OCT) and optical Doppler tomography (ODT) map epithelial, subepithelial, and vascular change throughout carcinogenesis. In vivo multiwavelength multiphoton (MPM) and second-harmonic generated (SHG) fluorescence techniques provided parallel data on surface and subsurface tissue structure, specifically collagen presence and structure, cellular presence, and vasculature. Images are diagnosed by two blinded, prestandardized investigators using a scale from 0 to 6 for all modalities. After sacrifice, histopathology is evaluated on a scale of 0 to 6. Imaging data are reproducibly obtained with good accuracy. Carcinogenesis-related structural and vascular changes are clearly visible to tissue depths of 2 mm. Sensitivity (OCT/ODT alone, 71 to 88%; OCT+MPMSHG, 79 to 91%) and specificity (OCT alone, 62 to 83%; OCT+MPMSHG, 67 to 90%) compare well with conventional techniques. Our conclusions are that OCT/ODT and MPM/SHG are promising noninvasive in vivo diagnostic modalities for oral dysplasia and malignancy.

Animals↗
PubMed2003

Near-infrared fluorescence contrast-enhanced imaging with intensified charge-coupled device homodyne detection: measurement precision and accuracy.

Fluorescence frequency-domain photon migration (FDPM) through tissue refers to the propagation of intensity-modulated fluorescent light that originates from tissue-laden fluorophores following illumination with an intensity-modulated excitation light source. FDPM measurements of modulation amplitude and phase are ultimately employed in an inversion algorithm for tomographic reconstruction of interior optical and fluorescent property maps that delineate disease enhanced with fluorescent contrast agent. Because the inverse problem is underdetermined, measurement precision and accuracy crucially impact its solution. Reported here are the precision and accuracy of FDPM measurements acquired using an intensified CCD homodyne detection system. By introducing 32 phase delays between the oscillators used to modulate the intensifier gain and light source intensity at 100 MHz, mean precision is maximized at +/-0.46% and +/-0.26 deg for measurements of modulation amplitude and phase, respectively. Measurement precision improves when the number of phase delays increases. Measurements of fluorescence modulation amplitude and phase, acquired from the surface of a tissue phantom at distances ranging between 0.71 and 3.6 cm from an incident excitation point source, exhibit a mean accuracy of 17% and 1.9 deg, respectively. Measurement accuracy deteriorates with increasing distance from the point source, but for distances up to 1.0 cm from the point source, measurements of fluorescence modulation amplitude and phase exhibit a mean accuracy of 5.4% and 0.30 deg, respectively.

Breast Neoplasms↗
PubMed2006

Normal macular thickness measurements in healthy eyes using Stratus optical coherence tomography.

OBJECTIVE: To report normal macular thickness measurements in healthy eyes using the latest commercially available optical coherence tomography (OCT) mapping software, version 3.0, from the Stratus OCT (OCT3). METHODS: Thirty-seven eyes from 37 healthy subjects underwent a complete ophthalmologic examination, including OCT. Six radial scans, 6 mm in length and centered on the fovea, were obtained using the OCT3. Retinal thickness was automatically calculated by OCT mapping software. Measurements were displayed as the mean and standard deviation for each of the 9 regions defined in the Early Treatment Diabetic Retinopathy Study. RESULTS: Foveal thickness (mean thickness in the central 1000-microm diameter area) and central foveal thickness (mean thickness at the point of intersection of 6 radial scans) on the OCT3 were 212 +/- 20 and 182 +/- 23 microm, respectively. Macular thickness measurements were thinnest at the center of the fovea, thickest within 3-mm diameter of the center, and diminished toward the periphery of the macula. The temporal quadrant was thinner than the nasal quadrant. Central foveal thickness was also manually determined as 170 +/- 18 microm, approximately 12 microm less than the value automatically obtained from the OCT3 software. There was no correlation between age and foveal thickness (P = .80). CONCLUSIONS: Mean foveal thickness measurements were 38 to 62 microm thicker than previously reported values, while mean central foveal thickness measurements were 20 to 49 microm thicker than previously published values. This discrepancy should be considered when interpreting OCT scans.

Adult↗
PubMed2004

Spatial mapping of droplet velocity and size for direct and indirect nebulization in plasma spectrometry.

Two novel laser-based imaging techniques centered on particle image velocimetry and optical patternation are used to map and contrast the size and velocity distributions for indirect and direct pneumatic nebulizations in plasma spectrometry. The flow field of droplets is illuminated by two pulses from a thin laser sheet with a known time difference. The scattering of the laser light from droplets is captured by a charge-coupled device (CCD), providing two instantaneous images of the particles. Pointwise cross-correlation of the corresponding images yields a two-dimensional velocity map of the aerosol velocity field. For droplet size distribution studies, the solution is doped with a fluorescent dye and both laser-induced florescence (LIF) and Mie scattering images are captured simultaneously by two CCDs with the same field of view. The ratio of the LIF/Mie images provides relative droplet size information, which is then scaled by a point calibration method via a phase Doppler particle analyzer. Two major findings are realized for three nebulization systems: (1) a direct injection high-efficiency nebulizer (DIHEN); (2) a large-bore DIHEN; and (3) a PFA microflow nebulizer with a PFA Scott-type spray chamber. First, the central region of the aerosol cone from the direct injection nebulizers and the nebulizer-spray chamber arrangement consists of fast (>13 and >8 m/s, respectively) and fine (<10 and <5 microm, respectively) droplets as compared to slow (<4 m/s) and large (>25 microm) droplets in the fringes. Second, the spray chamber acts as a momentum separator, rather than a droplet size selector, as it removes droplets having larger sizes or velocities. The concepts and results presented in this research may be used to develop smart-tunable nebulizers, for example, by using the measured momentum as a feedback control for adjusting the nebulizer, i.e., its operating conditions, its critical dimensions, or both.

Journal Article↗
PubMed2004

Electrotonic load triggers remodeling of repolarizing current Ito in ventricle.

A change in activation sequence electrically remodels ventricular myocardium, causing persistent changes in repolarizing currents (T-wave memory). However, the underlying mechanism for triggering activation sequence-dependent remodeling is unknown. Optical action potentials were mapped with high resolution from the epicardial surface of the arterially perfused canine wedge preparation (n = 23) during 30 min of baseline endocardial stimulation, followed by 40 min of epicardial stimulation, and, finally, restoration of endocardial stimulation. Immediately after the change from endocardial to epicardial stimulation, phase 1 notch amplitude of epicardial cells was attenuated by 74 +/- 8% (P < 0.001) compared with baseline and continued to diminish during the period of epicardial pacing, suggesting progressive remodeling of the transient outward current (Ito). When endocardial pacing was restored, notch amplitude did not immediately recover but remained attenuated by 23 +/- 10% (P < 0.001), also consistent with a remodeling effect. Peak Ito current measured from isolated epicardial myocytes changed by 12 +/- 4% (P < 0.025), providing direct evidence for Ito remodeling occurring on a surprisingly short time scale. The mechanism for triggering remodeling of Ito was a significant reduction (by 14 +/- 4%, P < 0.001) of upstroke amplitude in epicardial cells during epicardial stimulation. Reduction in upstroke amplitude during epicardial pacing was explained by electrotonic load on epicardial cells by fully repolarized downstream endocardial cells. These data suggest a novel mechanism for triggering electrical remodeling in the ventricle. Electrotonic load imposed by a change in activation sequence reduces upstroke amplitude, which, in turn, attenuates Ito according to its known voltage-dependent properties, triggering downregulation of current.

Action Potentials↗
PubMed2002

Incremental training increases the plasticity of the auditory space map in adult barn owls.

The plasticity in the central nervous system that underlies learning is generally more restricted in adults than in young animals. In one well-studied example, the auditory localization pathway has been shown to be far more limited in its capacity to adjust to abnormal experience in adult than in juvenile barn owls. Plasticity in this pathway has been induced by exposing owls to prismatic spectacles that cause a large, horizontal shift of the visual field. With prisms, juveniles learn new associations between auditory cues, such as interaural time difference (ITD), and locations in visual space, and acquire new neurophysiological maps of ITD in the optic tectum, whereas adults do neither. Here we show that when the prismatic shift is experienced in small increments, maps of ITD in adults do change adaptively. Once established through incremental training, new ITD maps can be reacquired with a single large prismatic shift. Our results show that there is a substantially greater capacity for plasticity in adults than was previously recognized and highlight a principled strategy for tapping this capacity that could be applied in other areas of the adult central nervous system.

Acoustic Stimulation↗
PubMed1983

Consequences of misrouting goldfish optic axons.

Cut optic axons regenerate into the goldfish tectum by indirect routes. To study the accuracy with which they terminate we made visuotectal maps before and immediately after cutting through the rostral tectum from its medial edge. This severed the normal pathway to medial tectum leaving intact only grossly misrouted axons. In fish mapped in this way, 3 months or more after contralateral optic nerve cuts, each initial map was essentially normal. After a tectal cut the electrical responses were usually weaker; but their receptive field positions proved to match very closely those noted previously for the same recording sites. The mean angular change was only 8.48 degrees and much of this could be accounted for by experimental errors. In fish mapped 6 months or more after attempts to cross-unite the optic tract brachia, initial maps were less regular. Some showed gross rearrangement of groups of terminals; and recognizable irregularities persisted in two fish mapped again after a further 4 or 5 months. However, maps made after tectal cuts in such fish were not noticeably less regular than the initial maps. Field position changes averaged 17.85 degrees. In both groups misrouted axons, filled with horseradish peroxidase after mapping and visualized in whole-mounts, crossed the tectum from lateral regions to reach the medial recording sites. The small field position changes in both groups confirm that axons from detectable terminals only among their retinal neighbours; nevertheless the irregular maps seen after tract cross-union lead us to expect the orderly arrangement of the normal pathway to contribute significantly to the precision of the normal map.

Animals↗
PubMed2005

Oxygen indicators and intelligent inks for packaging food.

The detection of oxygen using optical sensors is of increasing interest, especially in modified atmosphere food packaging (MAP), in which the package, usually containing food, is flushed with a gas, such as carbon dioxide or nitrogen. This tutorial review examines the ideal properties of an oxygen optical sensor for MAP and compares them with those developed to date, including the most recent advances. The basic technologies underpinning the different indicator types are described, examples given and their potential for application in MAP assessed. This tutorial review should be of interest to the MAP industry and researchers in optical sensors and oxygen sensing.

Colorimetry↗
PubMed2005

Pretarget sorting of retinocollicular axons in the mouse.

The map of the retina onto the optic tectum is a highly conserved feature of the vertebrate visual system; the mechanism by which this mapping is accomplished during development is a long-standing problem of neurobiology. The early suggestion by Roger Sperry that the map is formed through interactions between retinal ganglion cell axons and target cells within the tectum has gained significant experimental support and widespread acceptance. Nonetheless, reports in a variety of species indicate that some aspects of retinotopic order exist within the optic tract, leading to the suggestion that this "preordering" of retinal axons may play a role in the formation of the mature tectal map. A satisfactory account of pretarget order must provide the mechanism by which such axon order develops. Insofar as this mechanism must ultimately be determined genetically, the mouse suggests itself as the natural species in which to pursue these studies. Quantitative and repeatable methods are required to assess the contribution of candidate genes in mouse models. For these reasons, we have undertaken a quantitative study of the degree of retinotopic order within the optic tract and nerve of wild-type mice both before and after the development of the retinotectal map. Our methods are based on tract tracing using lipophilic dyes, and our results indicate that there is a reestablishment of dorsoventral but not nasotemporal retinal order when the axons pass through the chiasm and that this order is maintained throughout the subsequent tract. Furthermore, this dorsoventral retinotopic order is well established by the day after birth, long before the final target zone is discernible within the tectum. We conclude that pretarget sorting of axons according to origin along the dorsoventral axis of the retina is both spatially and chronologically appropriate to contribute to the formation of the retinotectal map, and we suggest that these methods be used to search for the molecular basis of such order by using available mouse genetic models.

Animals↗
PubMed1992

From basis functions to basis fields: vector field approximation from sparse data.

Recent investigations (Poggio and Girosi 1990b) have pointed out the equivalence between a wide class of learning problems and the reconstruction of a real-valued function from a sparse set of data. However, in order to process sensory information and to generate purposeful actions living organisms must deal not only with real-valued functions but also with vector-valued mappings. Examples of such vector-valued mappings range from the optical flow fields associated with visual motion to the fields of mechanical forces produced by neuromuscular activation. In this paper, I discuss the issue of vector-field processing from a broad computational perspective. A variety of vector patterns can be efficiently represented by a combination of linearly independent vector fields that I call "basis fields". Basis fields offer in some cases a better alternative to treating each component of a vector as an independent scalar entity. In spite of its apparent simplicity, such a component-based representation is bound to change with any change of coordinates. In contrast, vector-valued primitives such as basis fields generate vector field representations that are invariant under coordinate transformations.

Animals↗
PubMed1985

Regional distribution of fast and slow geniculo-cortical relay cells (GCR-cells) within the rat's dorsal lateral geniculate nucleus (LGNd).

Unit activity of 135 GCR-cells in rat's LGNd was recorded extracellularly using micropipettes filled with trypan blue for subsequent marking of recording sites. The latencies of responses to electrical stimulation of the optic chiasma (OX) were mapped, and in accordance with Fukuda (1973) each GCR-cells was classified as fast or slow. Fast cells predominate in the rostroventromedial subregion and slow cells in the caudal part. Both fast and slow subtypes were found in the remaining rostrodorsolateral zone where slow cells made up two thirds of the subsample. The results fit the suggestion that in the LGNd of rats a fast retino-geniculate pathway relays in the ventromedial region, whereas slow pathways exist in the caudal as well as in the dorsolateral zone.

Animals↗