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Heavy metals: sources and environmental presence.

The Istituto Superiore di Sanità has for many years been involved in the problem of environmental pollution caused by heavy metals. A brief review of the sources of this pollution is first presented which also includes the presence in nature of some heavy metals which are among the most dangerous. Data gathered by the authors is then presented from sampling of the emissions of urban solid waste (USW) incinerators and from environmental monitoring programs in different areas in Italy according to four categories: urban, industrial, rural and mixed.

Environmental Pollutants↗

Food discard practices of householders.

Food discard patterns and reasons were determined for a sample of 243 households in Oregon. Personal interviews were conducted, and 7-day records of discards were collected. Discards over a 3-day period also were collected from a subsample of 50. The householder's estimate of amount, converted from measures to grams using food composition tables, was found to be 97% of the actual grams of food, as weighed in the laboratory. Households discarded an average of 1,587 gm ($2.88) food in a 7-day period on the basis of the 79% completed usable records. Major reasons were poor quality for fruits and vegetables; storage time for meat, fish, and poultry; non-use of leftovers for combination dishes; and plate waste for cereals and dairy products. Twenty-nine percent of the discarded food (by cost) was considered to be unsafe to eat by the householder. Aesthetic factors dominated decisions by the 18- to 25-year age group, but experiences related to food storage were the basis for decisions by half of the respondents more than 65 years old. Discards increased with number of members in the household and were influenced by age of children. Household income was not linearly related to amount of discard. As household refrigerator temperatures increased from 1.7 degrees C to 20 degrees C, the amount of discards also increased. Consumers generally lacked criteria for evaluating the safety of foods.

Adolescent↗

[Behavior of microorganisms in wastes from medical practice and household refuse deposited in a model landfill].

Refuse from medical consulting rooms, a mixture of those refuse with municipal refuse (1:10), and municipal refuse were disposed aerobically and roughly anaerobically for over six months in a model landfill. Survival, proliferation, and transportation of microorganisms were estimated at different periods of time. Concentrations of aerobic bacteria and hyphomycetes decreased during the first weeks of deposition but remained later almost unchanged. Concentrations of nonsporeforming indicator bacteria (Escherichia coli, feacal streptococci) decreased more strongly, and E. coli could not be found at latest after 23 weeks. In municipal refuse alone and mixed with refuse from medical consulting rooms, Pseudomonas aeruginosa proliferated temporarily. Leaching of microorganisms from the model landfill was observed in the whole course of the disposal period.

Aerobiosis↗

[Microbiological studies of waste from medical practice and household refuse].

Microbiological investigations were made during a one year period on refuse from consulting rooms of general practitioners, E.N.T.-specialists, dermatologists, dentists, and veterinarians. Concentrations of total aerobic bacteria, hyphomycetes, yeasts, actinomycetes, indicator bacteria (Escherichia coli, feacal streptococci, anaerobic sulfite reducing spore-forming bacteria), of some facultative pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus), and pathogens (Salmonella sp.) were estimated. The refuse from medical consulting rooms showed definitely differences in microbiological properties. Usually the highest counts of microorganisms were found in the refuse from veterinarian consulting rooms. In generally, refuse from medical consulting rooms had lower microbial counts as compared to the municipal refuse. Feacal indicators and facultative pathogenic bacteria, however, were found more frequently, and usually at higher concentrations in refuse from medical consulting rooms. Salmonella sp. was not found in the refuse samples under test. Conclusively, refuse from medical consulting rooms should be handled with caution. However, the results of this study do not indicate those kinds of refuse as a source of acute hygienic risk.

Bacteria↗

Monitoring trends in food utilization: application of an archaeologcial method.

Archaeological methods were utilized to monitor trends in food purchase and utilization behavior in Tucson, Arizona households through the analysis of household refuse. As a supplement to traditional methods of collecting data on food consumption, the study of household refuse can provide information on short-term trends in behavior and on food discard. Between 1973 and 1975, households studied decreased the amount of food brought into the home and decreased their purchase of selected animal protein foods. Lower-income tracts, in addition, increased their purchase of grain products, substituted cheaper for more expensive food intems, and decreased food discard. While these data do not reflect nutritional status, they provide a unique view of the socioeconomic and behavioral patterns that in large part determine nutritional status.

Archaeology↗

Introduction and reisolation of selected gram-positive bacteria from fermented edible wastes.

A fermentation process using Lactobacillus acidophilus added to edible food wastes was evaluated for its bactericidal action on selected gram-positive organisms. The Lactobacillus fermentation converts food wastes into an animal feed ingredient. In this study, 5 gram-positive bacteria of zoonotic importance were individually tested. These organisms were: Group E Streptococcus, Erysipelothrix rhusiopathiae, Clostridium perfringens, Corynebacterium pseudotuberculosis, and Listeria monocytogenes. For each experiment, Lactobacillus was first mixed into ground waste; one of the test organisms was then inoculated and mixed. This mixture was divided among eight 5.5-L containers and incubated (duplicates) at 5 C, 10 C, 20 C, and 30 C for 96 hours. Internal waste temperature, reduction-oxidation, and pH were monitored. Waste samples were taken initially and at subsequent 24-hour periods. Qualitative and quantitative recoveries of the test bacteria were attempted for each sample. Group E Streptococcus was reisolated in increasing numbers at all temperatures throughout the fermentation period. Erysipelothrix rhusiopathiae was recovered throughout the 96-hour period at 5 C; at 10 C it was recovered at 24 hours but not at 48 hours. Erysipelothrix rhusiopathiae was killed by 24 hours at 20 C and 30 C fermentation temperatures. Clostridium perfringens survived the entire test period at 5 C, 10 C, and 20 C; it was killed by 72 hours at 30 C. Neither Corynebacterium pseudotuberculosis nor Listeria monocytogenes was reisolated at any temperature at any time.

Animal Feed↗

Public health importance of market meat exposed to refuse flies and air-borne microorganisms.

Abbatoir meats sold in the open at the Ibadan market stalls were swabbed to investigate the presence of any microorganism. Flies were caught on carcases being transported from the abbatoir in open vehicles to meat stalls and also at meat stalls and nearby refuse using fly catching nets. Major flies caught were Musca domestica; Culicoides species, Chrysomia and Fannia cannicularis. Stophylococcus aureus, Salmonella and Escherichia coli were the bacteria isolated from.

Abattoirs↗

[Technology of composting].

The technology of composting must guarantee the material-chemical, biological and physical-technical reaction conditions essential for the rotting process. In this, the constituents of the input material and the C/N ratio play an important role. Maintaining optimum decomposition conditions is rendered difficult by the fact that the physical-technical reaction parameters partly exclude each other. These are: optimum humidity, adequate air/oxygen supply, large active surface, loose structure with sufficient decomposition volume. The processing of the raw refuse required to maintain the physical-technical reaction parameters can be carried out either by the conventional method of preliminary fragmentizing, sieving and mixing or else in conjunction with separating recycling in adapted systems. The latter procedure obviates some drawbacks which mainly result from the high expenditure required for preliminary fragmentation of the raw refuse. Moreover, presorting affords the possibility of reducing the heavy-metal content of the organic composing fraction and this approaches a solution to the noxa disposal problem which at present stands in the way of being accepted as an ecological waste disposal method.

Carbon↗

[Material recovery from waste].

Reclamation of raw material from refuse is demonstrated by two practical examples. In numerous countries sorting plants were set up at considerable expense for the purpose of reclaiming plastic material from household refuse. Most of these plants are struggling more or less with difficulties as a result of technical and particularly economic problems. A market for reclaimed raw materials scarcely exists. This applies most particularly to the reclamation of plastics from household refuse. As long as the operating costs, especially the energy costs, exceed the proceeds obtained for regranulated material, "recycling" will be to no purpose. At present, reclaiming energy from refuse seems to hold out better prospects of success.

Austria↗

Introduction and reisolation of selected gram-negative bacteria from fermented edible wastes.

A Lactobacillus fermentation process, using edible food wastes, was tested for its ability to eliminate selected bacterial pathogens. This fermentation process converts food wastes into a feed ingredient for animal consumption. Six gram-negative bacterial pathogens of potential zoonotic importance were tested. These experimental organisms were: Salmonella enteritidis serovar typhimurium, S enteritidis serovar anatum, S cholerae-suis, Yersinia enterocolitica, Y pseudotuberculosis, and Pasteurella multocida. Each organism was introduced into ground waste that had been previously inoculated with L acidophilus, and was mixed. This mixture was divided among 8 containers, and was incubated in duplicate at 5 C, 10 C, 20 C, and 30 C for 96 hours. The temperature of the reactant containers, reduction-oxidation potential, and pH were monitored. Waste samples were obtained initially and subsequently at 24-hour periods for 96 hours. Qualitative and quantitative recovery attempts from each sample were made for the introduced gram-negative bacteria. Pasteurella multocida and the S enteritidis serovars typhimurium and anatum survived the fermentation at 5 C and 10 C, but were killed after 48 hours at 20 C and 30 C. Salmonella cholerae-suis survived at 5 C, but was destroyed by 72 hours at the remaining temperatures. Yersinia enterocolitica was viable through 70 hours, but was killed by 96 hours. Yersinia pseudotuberculosis was not reisolated at any temperature.

Animal Feed↗

Moulds in containers with biological wastes.

The collection of biological wastes in separate bio-containers can lead to a favoured development of thermophilic and thermotolerant moulds, especially of mucoraceous species and aspergilli, among which the human pathogen A. fumigatus is especially frequent. The abundantly produced spores are released into the air and can evoke severe infections in persons with immune-deficiencies. In two series of experiments it was demonstrated that the following procedures can reduce the number of spores in the air in the bio-containers above the biological wastes: (1) wrapping the wastes in portions in newsprint: the number of colony-forming units (CFU) decreases for about 50-70%; (2) cleaning of the container after each emptying with diluted vinegar: the number of CFU is reduced for up to 80%; (3) placing the container at shady sites: the temperature of the air inside the bio-containers at shady sites is approximately 5-8 degrees C lower than at sunny places with the consequence that the number of CFU in the air above the biological wastes is decreased. Based on these results principles for the handling of biological wastes are set up.

Air Microbiology↗