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Location of motoneurons innervating the musculus sphincter cloacae in the domestic fowl.

Horseradish peroxidase was injected into the musculus (m.) sphincter cloacae through the mucosa from the lumen side of the cloaca in the domestic fowl. Labeled neurons were found in the ventrolateral area of the ventral horn from the lumbosacral segments 7-9. The craniocaudal distribution of labeled neurons overlapped with that of hindlimb motoneurons. The m. sphincter cloacae is innervated by motoneurons sending axons to the sphincter cloacae either through the connexus caudalis branching from the caudal coxal nerve of the sacral plexus or through the lateral caudal nerve arising from the pudendal plexus.

Animals↗

Arginine vasotocin gene expression in neuroendocrine, reproductive and gastrointestinal tissues of the domestic fowl: detection by reverse transcriptase polymerase chain reaction.

Arginine vasotocin gene transcripts in various tissues of the domestic fowl were detected by reverse transcriptase polymerase chain reaction followed by Southern blot analysis using a 209 bp fragment from the 3'-region of a cDNA encoding chicken arginine vasotocin as the probe. Relatively strong signals were observed with hypothalamic, adenohypophysial and proventricular RNA as the starting material. Lesser signals were obtained from RNA isolated from shell gland, adrenal gland, post-ovulatory follicles and ovarian thecal cells. Arginine vasotocin gene transcripts were undetectable in the posterior pituitary gland, small intestine and large intestine. These results suggest that in addition to its well-known antidiuretic and oxytocic actions, arginine vasotocin may act as a local neuromodulator or mediator and have other important autocrine or paracrine actions in non-hypothalamic tissues.

Animals↗

Histopathological changes in the proventriculus of fowl given experimental monospecific infection with Acuaria spiralis.

The pathological changes in the proventriculi of fowls given monospecific experimental infection with Acuaria spiralis were characterized in the initial stages by an acute inflammation due to the migration of 3rd and 4th stage larvae. There was a severe non-keratinizing squamous cell metaplasia of the lining epithelium with pronounced granulocytic infiltration at 4-8 days post-infection. Multiple lymphoid nodules were evident in the lamina propria from 12 days post-infection. As the disease progressed there was extensive fibroplasia in the organ. By 50-100 days post-infection pedunculated fibro-adenamatoid growths developed in the mucosa obliterating the entire lumen of the organ. A mortality rate of 29.16% of chicks (maximum on the 3rd week post-infection) was observed.

Animals↗

Progress on control of northern fowl mites on caged laying hens.

Recent research on methods for controlling the northern fowl mite (NFM) on caged laying hens is reviewed. On inanimate objects, NFM may be controlled by fumigation with methyl bromide, increased temperature (49 degrees C for 1 h), or decreased temperature (-20 degrees C for 5 days). dipping chickens in aqueous suspensions of carbaryl or stirofos controlled NFM for 6 weeks without exceeding current egg tolerance residues. Feeding chickens reasonable concentrations of systemic pesticides registered for use on dairy cattle in the U.S.A., registered anticoccidials, zinc bacitracin, avermectins, and diflubenzuron did not produce systemic control of NFM. Chickens do develop immunity to NFM. The potential for further development and adoption of these techniques should provide the poultry industry with effective alternatives to existing chemical sprays and dusts.

Animals↗

Effect of Raillietina echinobothrida infection on blood values and intestinal tissues of domestic fowls of Bangladesh.

An examination of the blood profile of domestic fowls infected with Raillietina echinobothrida revealed anaemia with a significant increase of total leukocyte counts and decrease of total serum protein. The pathological changes in the intestines were characterised by villous atrophy, enteritis with cellular infiltration and formation of characteristic granulomas.

Anemia↗

The efficacy of praziquantel (Droncit R) against Raillietina tetragona (Molin, 1958) in domestic fowl.

The efficacy of different dose rates of Praziquantel (Droncit R) in tablet and liquid (injectable) form against Raillietina tetragona in the domestic fowl was studied. A dose of 10 mg kg-1 of Praziquantel in tablet form and 0.15 ml kg-1 in liquid form was found to be effective against R. tetragona infection irrespective of age, sex and intensity of infestation of the host. Intramuscular (IM) administration of liquid Praziquantel was found to be more effective than subcutaneous (SC) administration. Susceptibility of the parasite to this drug increased with age.

Animals↗

Isolation and characterization of a tyrosinase from the skin of the white silky fowl (Gallina lanigera) employing copper saturated diethylaminoethyl-cellulose.

Tyrosinase was purified to apparent homogeneity from the skin of the White Silky fowl (Gallina lanigera). Under alkaline conditions copper ions from a complex with the diethylaminoethyl moiety of DEAE-cellulose, allowing the purification of the enzyme in a one step chromatography. In polyacrylamide gel electrophoresis containing sodium dodecyl sulfate the purified tyrosinase displays a single band with a relative molecular mass of 66,000 Da. The crystalline tyrosinase was obtained from 32-33% saturated solution of ammonium sulfate. The enzyme catalyzes the o-hydroxylation of L-tyrosine to L-dihydroxyphenylalanine as well as the oxidation of pyrocatechol to o-benzoquinone. For the pyrocatechol oxidation the Michaelis Menten constant Km, is calculated to be around 1.19 x 10(-3) M.

Animals↗

Patterns of amino acid enzyme in domestic fowl breast and leg muscle during development.

Activities of alanine and aspartate transaminases, glutamine synthetase, adenylate deaminase, glutamate and xanthine dehydrogenases and lactate dehydrogenase were measured in leg and breast muscles of developing chicks from day 10 in ovo to day 5 of free life, and compared with measurements for adult hens. Xanthine dehydrogenase activity was low in both muscles with adult levels attained on day 15 in ovo. Glutamine synthetase for chicks was maintained higher during development than for adults in both muscles. Minor differences were observed between both muscles in all enzymes tested up to day 18. With low embryonic values and important rises before hatching, the differences were initiated in the posthatching period. Important differences were observed between adult levels of activity. Leg muscle revealed higher enzyme values except for lactate dehydrogenase and indistinguishable levels for adenylate deaminase and xanthine dehydrogenase in both muscles. Alanine, instead of glutamine, is postulated as the main nitrogen transport between muscle and liver in the domestic fowl.

AMP Deaminase↗

Tissue glycogen and lactate handling by the developing domestic fowl.

The levels of glycogen and lactate in liver, intestine, yolk sac membrane and leg and breast muscle of domestic fowl from day 10 of "in ovo" development to day 5 after hatching compared with adults have been measured and compared with the circulating concentrations in blood of glucose and lactate. Glycogen stores in most tissues increased before hatching to attain a minimum around the eclosion and then increased to adult values in muscle and liver. Lactate maintained its plasma concentrations with higher effectiveness than plasma glucose, which increased steadily up to adult levels from hatching. The study of tissue vs plasma lactate concentration ratios suggests a general activation of lactate metabolism from hatching, coinciding with the ingestion of carbohydrate-based food. Both muscles studied, as well as intestine, seem to be net lactate producers; blood cells can speculatively be considered as lactate users and liver maintains its concentration of lactate very close to that of plasma, suggesting a fast utilization of this material as well as liver being the main site for control of circulating lactate.

Aging↗

Effect of starvation and a protein diet on the amino acid metabolism enzyme activities of the organs of domestic fowl hatchlings.

The activities of alanine and aspartate transaminases, adenylate deaminase, glutamine synthetase and glutamate and xanthine dehydrogenases have been measured in liver, yolk sac membrane, intestine and breast and leg muscle of domestic fowl hatchlings receiving for 3 or 5 days either a standard diet or hard boiled eggwhite as well as in 3 or 5 days starved animals. The patterns of activation of amino acid metabolism enzymes were fully comparable in protein-fed and starved groups with respect to fed controls; the differences with respect to the latter became more marked in 5- than in 3-days old chicks. In 5-days old chicks intestine alanine transaminase activity increased in parallel to that of liver in protein-fed animals but not in those starved, in agreement with an enhanced alanine transfer between both organs under this situation. Both, starvation and protein-feeding, induced a general decrease in the amino acid metabolizing ability of muscle. Glutamine (but not alanine) synthetizing capabilities were enhanced.

AMP Deaminase↗

Comparative distribution of glycosaminoglycans and proteoglycans in the reproductive tissues of the female fowl.

The objective of this work was to survey and compare the composition of the parts of the reproductive system of the female fowl in glycosaminoglycans and proteoglycans. Those parts analyzed were ovary, infundibulum, magnum, isthmus, shell gland and vagina. Methods of analysis included cellulose acetate electrophoresis, infrared spectroscopy, colorimetry, amino acid determination and scanning electron microscopy. Concentrations of glycosaminoglycans were higher in vagina, ovary, infundibulum and isthmus than in shell gland and magnum. Glycosaminoglycans may be important in those parts of the reproductive tract which contribute membraneous and mucosal material to the descending egg, and where fertilization of the egg occurs.

Amino Acids↗

A comparative study of the structure of egg-white riboflavin binding protein from the domestic fowl and Japanese quail.

1. The riboflavin binding proteins from domestic fowl and Japanese quail have been isolated and their structures compared by circular dichroism, fluorescence and peptide mapping. 2. The two proteins have similar secondary structures, but differ in their tertiary structures as reflected in the environments of aromatic amino acid side chains. 3. Differences in amino acid sequence between the proteins are indicated by the digestion patterns obtained with thermolysin, chymotrypsin and V8 proteinase from Staphylococcus aureus. Both proteins are resistant to digestion by trypsin.

Animals↗

Neurotransmitter effects on in vitro prolactin and growth hormone release from fowl pituitary glands: dopamine and noradrenaline.

Pituitary glands and hypothalami from broiler fowl heads were incubated alone or together with dopamine, noradrenaline or monoaminergic drugs (apomorphine, pimozide, phentolamine, isoproterenol and propranolol). The basal release of both prolactin and growth hormone was not affected by any of these amines or aminergic drugs. The co-incubation of pituitary glands with whole hypothalami consistently stimulated prolactin and growth hormone secretion. Apomorphine inhibited hypothalamus-induced prolactin and growth hormone release and the response was blocked by pimozide. Noradrenaline markedly stimulated hypothalamus-induced prolactin release, an effect antagonised by phentolamine. Thyrotrophin-releasing hormone (TRH) stimulated the release of prolactin and this stimulation was reversed, in a dose-related manner, by dopamine. TRH also increased the release of growth hormone, in the presence or absence of dopamine. These results demonstrate inhibitory dopaminergic effects on prolactin and growth hormone secretion and stimulatory noradrenergic effects on prolactin release. These aminergic effects may be mediated at pituitary and/or hypothalamic sites.

Animals↗

Effects of putative neurotransmitters on release of prolactin from pituitary glands of the domestic fowl co-incubated with hypothalamic tissue.

Pituitary glands and hypothalami from broiler fowl heads were incubated alone or together with histamine, gamma-aminobutyric acid (GABA) or acetylcholine (ACh) as well as with catecholamines or neurotransmitter antagonists. Histamine and ACh stimulated, whereas GABA inhibited, the hypothalamus-induced release of prolactin, responses blocked by their specific antagonists. The dopamine antagonist pimozide, but not adrenergic (both alpha and beta), serotoninergic or cholinergic antagonists, prevented the actions of histamine and GABA. None of the antagonists except the cholinergic blocker, atropine, affected ACh-induced release of prolactin. Neither histamine nor ACh prevented inhibition of prolactin release by dopamine or stimulation of prolactin release by noradrenaline. GABA did not affect the response to noradrenaline. Furthermore, histamine, GABA and ACh had no effects on thyrotrophin releasing hormone-stimulated release of prolactin directly at the pituitary level. These results suggest that histamine and GABA affect prolactin release from chicken pituitaries in vivo by modifying the activity of the dopaminergic system. Acetylcholine may stimulate the secretion of prolactin releasing factor from the hypothalamus.

Acetylcholine↗

Opiate participation in thyroid hormone regulation in domestic fowl.

Administration of morphine sulfate (at 5 and 50 mg/kg) to immature domestic fowl lowered the circulating thyroxine (T4) concentration in a dose- and time-dependent manner. The inhibitory effect of morphine on T4 secretion was mediated via opiate receptors, since it was completely blocked in the presence of naloxone (50 mg/kg). The possibility that T4 secretion may be under tonic opioid inhibition was suggested by a stimulatory effect of naloxone on plasma T4 concentrations. Morphine sulfate (50 mg/kg) enhanced plasma triiodothyronine concentrations, an effect that was completely suppressed in the presence of naloxone. These results demonstrate differential effects of opiates on T4 and T3 concentrations and suggest that opiates tonically inhibit T4 release and accelerate its rate of peripheral monodeiodination.

Animals↗

5-Hydroxytryptamine in the spinal cord of the domestic fowl.

5-Hydroxytryptamine (5HT) immunoreactive fibers and varicosities are present in the gray and white matters of the adult domestic fowl spinal cord. These immunoreactive structures are densest in laminae I and II, the area around the central canal, and in the ventral horn. 5HT fibers and varicosities surround certain laminae I and II cells and large ventral horn cells. The apparent intimate relationship between dorsal horn cells and numerous 5HT structures may render them good models to study the possible role of 5HT in the modulation of nociception in the dorsal horn.

Animals↗

Food and water intake responses of the domestic fowl to norepinephrine infusion at circumscribed neural sites.

The effect on food and water intake of injection of norepinephrine into circumscribed brain sites of the domestic fowl was investigated. Injection of norepinephrine into sites throughout the preoptic area caused reliable increases in food intake. Food intake was also increased by injection of norepinephrine in the ventromedial nucleus, paraventricular nucleus, and medial septal sites. Food intake was decreased by injections near the lateral septal organ and the anterior portion of both the nucleus reticularis superior, pars dorsalis, and the tractus occipitomesencephalicus. Within the preoptic area, water intake was increased at basolateral sites but was inconsistently affected at more medial sites. No consistent trends were noted at sites examined outside the preoptic-hypothalamic area.

Animals↗