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At least 739 records · Page 41Linked to original sources
PubMed1980

Fluorescein angiography in diabetic children.

In 63 diabetic children, aged 10--14 years, with normal ophthalmoscopy, fluorescein angiography revealed slight diabetic retinopathy in maximally three, aged 12--14 years. These findings are discussed in relation to the controversial results published in a few other fluorescein angiographic studies on diabetic children. It is concluded that normal ophthalmoscopy does not exclude the presence of diabetic retinopathy and that fluorescein antiography is a valuable method in the early diagnosis of diabetic retinopathy.

Adolescent↗
PubMed1986

Adverse reactions to fluorescein angiography.

Adverse reactions to ophthalmic patients during 9909 fluorescein angiographies during 9 years were registered. Nausea (4.6%) and vomiting (1.3%) were the most common untoward reactions. Allergic skin manifestations occurred in 48 patients, and 5 patients complained of shortness of breath. 56 patients (0.6%) felt dizzy during or immediately after the investigation. Nine patients complained of chest pain, three of whom developed myocardial infarction. Sixteen patients collapsed during the procedure. One healthy male, 42-year-old, collapsed after the injection of fluorescein during angiography, and electrocardiogram showed an asystole of 24 seconds. Otherwise, the electrocardiograms registered on 100 consecutive patients did not reveal any systematic changes in heart rate or rhythm during fluorescein angiography.

Adolescent↗
PubMed1987

Centripetal movement of fluorescein dextrans in the cornea: relevance to arcus.

The centripetal movement of fluorescein and fluorescein-labelled dextrans (4 to 150 kD) from sclera or cut edge of the cornea was determined in isolated rabbit corneas at 4 and 24 h. Corneas were divided into 5.5 mm diameter central core, inner 5.5 to 8 mm donut, 8 to 12 mm peripheral donut and, where applicable, scleral rim. For all molecules greater than sodium fluorescein (376 D) tracer concentrations in the 5.5 mm core and the 5.5 to 8 mm donut were equal. Without sclera rim, the more central portions of the cornea (5.5 mm core and 5.5 to 8 mm donut) had tracer concentrations equal to those of corneas-with-sclera for all tracers greater than 10 kD. The tracer concentrations in the central cornea were the same in the presence or absence of sclera. The data indicate a physiological barrier to the lateral diffusion of molecules greater than 10 kD between the peripheral and more central cornea.

Animals↗
PubMed2003

Measurement of Chlamydia pneumoniae-specific immunoglobulin A (IgA) antibodies by the microimmunofluorescence (MIF) method: comparison of seven fluorescein-labeled anti-human IgA conjugates in an in-house MIF test using one commercial MIF and one enzyme immunoassay kit.

For the serological diagnosis of acute Chlamydia pneumoniae infection, the microimmunofluorescence (MIF) test is the most commonly used method and also the "gold standard" for the measurement of immunoglobulin G (IgG) and IgM antibodies. The role of IgA antibodies in diagnosis has not been established. Commercially available fluorescein-labeled anti-human IgA conjugates have not been systematically compared to each other, and this situation may cause considerable variations in IgA results. Therefore, we tested 261 serum samples from 122 patients with pneumonia for IgA antibodies by using six alpha-chain-specific anti-IgA conjugates in our in-house MIF test, one commercial MIF test, and one enzyme immunoassay (EIA). Interfering IgG antibodies were removed with Gullsorb reagent before the measurement of IgA antibodies. Altogether, 14 significant IgA antibody increases in serum samples between the acute phase and the convalescent phase were detected by at least one of the conjugates in the MIF test, while no increases were found in the IgA EIA. Only one patient showed a significant IgA antibody increase with all of the fluorescein-labeled conjugates. Five significant titer changes were detected by at least two conjugates, and in nine instances, the titer increase was detected by one conjugate only. The titer agreement indicated by kappa coefficients was very good or good for all of the fluorescein-labeled conjugates and the EIA with low antibody titers but decreased with increasing titers.

Adult↗
PubMed1987

Evaluation of a direct fluorescein-conjugated monoclonal antibody for detection of cytomegalovirus in centrifugation culture.

A fluorescein-conjugated murine monoclonal antibody (MAb) reactive with cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 188 specimens, 90 were positive for CMV in centrifugation culture. The fluorescein-conjugated MAb detected CMV in 86 of 90 (95%) specimens at 16 h postinoculation, and 88 of 90 (98%) were positive at 36 h. The fluorescein-conjugated MAb can be used in a direct immunofluorescence assay that can be completed in 15 min following cover slip fixation. Use of this antibody in centrifugation culture provides a convenient and rapid assay for the identification of CMV.

Antibodies, Monoclonal↗
PubMed1989

Rapid detection of herpes simplex virus with fluorescein-labeled Helix pomatia lectin.

The use of fluorescein-conjugated Helix pomatia lectin was shown to be as effective as fluorescein-conjugated monoclonal antibody reagents for the detection and differentiation of herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) in MRC-5 cell culture. Cells infected with HSV-1 generally displayed a pattern of nongranular or diffuse fluorescence, while cells infected with HSV-2 were identified by the production of fluorescent grains and flecks. This unique nonimmunological reagent, when used in combination with low-speed centrifugation, provides a remarkably specific, sensitive, rapid, and cost-effective means to detect HSV-infected MRC-5 or BHK-21 cells as early as 20 h postinoculation. In contrast to the immunofluorescence method, the serotypes of HSV can be differentiated with only one fluorescein-H. pomatia reagent in MRC-5 cell cultures.

Animals↗
PubMed1982

Use of fluoresceinated Epstein-Barr virus to study Epstein-Barr virus-lymphoid cell interactions.

As a direct approach to visualize Epstein-Barr virus (EBV) binding to its cellular receptors and to learn more on the nature of this binding, virus preparations were conjugated to fluorescein isothiocyanate and used to detect EBV receptors on lymphoid cells. Different enzymatic and chemical treatments were also applied either to the virus or to target cells or to both, and the effect of these treatments on virus binding was then examined. The results obtained show that: (i) EBV can be fluoresceinated without affecting its infectivity or cell binding ability, and the fluoresceinated virus represents an important tool to investigate the biology and nature of EBV interactions with its cellular receptors; (ii) the two virus strains (P3HR-1 and B95-8) share common receptors on Raji cells; (iii) protease treatment of EBV or target cells abrogates virus binding; (iv) EBV receptors regenerate after removal of the protease, and this regeneration is inhibited by cycloheximide or sucrose; (v) EBV particles bear concanavalin A receptors, and this lectin hinders the interaction of the virus with its cellular receptors; (vi) neuraminidase treatment, various monosaccharides, ovalbumin, and glycopeptides derived from EBV or cell surface do not inhibit virus binding. Taken together, the above data also demonstrate that some cellular and viral surface (glyco-) proteins are required for EBV binding to its targets.

Cell Line↗
PubMed1982

SLE retinopathy: evaluation by fluorescein angiography.

Fifty-two patients with systemic lupus erythematosus (SLE) were examined by fluorescein angiography, and retinopathy was detected in 15. Three patterns of retinopathy were discerned: 4 patients had disc vasculitis, 6 had multiple cotton-wool spots, and 5 had a normal fundal appearance but fluorescein leakage on angiography. One patient had arterial occlusive disease with retinal neovascularisation and another had extensive venous disease. With 3 exceptions retinopathy was found only in patients with active SLE. No association was discovered between retinopathy and cerebral disease; in particular, fluorescein angiography did no assist the diagnosis of mild cerebral lupus.

Adult↗
PubMed1991

Laser photocoagulation control of diabetic macular oedema without fluorescein angiography.

This study included 40 eyes in 22 diabetic patients with focal macular oedema. Laser photocoagulation was directed at decompensated or leaking microvascular lesions clinically detected without using pretreatment fluorescein angiograms. Post-treatment fluorescein angiograms performed after adequate clinical control of disease showed complete resolution of the macular oedema in 25 eyes (62.5%), whereas persistent leakage from microvascular lesions closer than 500 microns from the centre of the foveola was noted in 15 eyes (37.5%). These were clinically detected during the pretreatment examination and were found not to impair or threaten the patient's vision. Our data confirm the clinical impression that fluorescein angiography is not necessary for effective treatment and should be used only if necessary.

Adult↗
PubMed1993

Aqueous flare and cells in eyes with retinal vein occlusion--correlation with retinal fluorescein angiographic findings.

The laser flare cell meter (LFCM) was used to evaluate alterations of the blood-aqueous barrier in eyes with retinal vein occlusion (RVO). In 42 eyes of 42 patients with RVO (30 branch vein occlusions, 12 central vein occlusions) aqueous flare and aqueous cells were determined and retinal fluorescein angiography was performed. Flare and cell values were compared with 59 normal age and sex-matched control eyes and correlated with fluorescein angiographic findings. Both aqueous flare (12.321 (SD 6.717) photon counts/ms) and aqueous cells (mean 3.37 cells/0.075 x 10(6)/l) were significantly increased in eyes with RVO in comparison with the normal control group (flare 4.288 (SD 1.144) photon counts/ms, cells 0.54 cells/0.075 x 10(6)/l, p < 0.0001 and p < 0.005). Flare values in eyes with central RVO (19.517 (SD 7.762) photon counts/ms) were significantly higher than in eyes with branch RVO (9.443 (SD 3.307) photon counts/ms, p < 0.0001). Significant correlations were found between flare values and area of RVO (r = 0.70, p < 0.0001) and between flare values and area of retinal non-perfusion (r = 0.76, p < 0.0001). There were also significant correlations between flare values and number of cotton wool spots (r = 0.54, p < 0.0002) and between cell counts and degree of cystoid macular oedema (r = 0.46, p < 0.006). Our findings show that aqueous protein concentration, as indicated by flare, and aqueous cells are increased in RVO and that changes of aqueous flare correlate with fluorescein angiographic findings that reflect the severity of RVO. Measurements with LFCM may be useful non-invasively to quantify changes of the ocular barrier functions in RVO.

Adult↗
PubMed1998

Fluorescein angiographic correlation of focal narrowing of retinal arterioles in glaucoma.

BACKGROUND: Recent studies have shown that focal narrowing of retinal arterioles in the parapapillary region occurs in eyes with optic neuropathies such as glaucoma. This study evaluated whether these vessel constrictions detected ophthalmoscopically have an equivalent in angiographic imaging of the fundus. METHODS: Fluorescein angiograms and colour wide angle fundus photographs of 33 patients with open angle glaucoma and 76 subjects with normal optic nerves were examined for focal narrowing of retinal arterioles. The angiograms had primarily been taken for other reasons such as age related macula degeneration. RESULTS: All focal narrowings of retinal arterioles detected on fundus photographs showed a localised constriction of vessel filling in the fluorescein angiograms. Degree of vessel narrowing on the fundus photographs and degree of constriction of the fluorescein vessel filling were significantly (p < 0.001) correlated with each other. CONCLUSIONS: Focal narrowing of retinal arterioles in the parapapillary region of eyes with optic neuropathies represents a real stenosis of the vessel lumen and is not due to an ophthalmoscopic artefact.

Aged↗
PubMed1998

Correlation between Octopus perimetry and fluorescein angiography after strontium-90 plaque brachytherapy for subfoveal exudative age related macular degeneration.

AIM: To evaluate the correlation between the central visual field and changes in fluorescein angiography and fundus photography in patients treated with strontium plaque radiotherapy for subfoveal exudative age related macular degeneration (AMD). METHODS: Octopus program 34 automated static perimetry, fluorescein angiography, and colour fundus photography were performed on 19 patients at baseline and at 12 months after strontium-90 plaque therapy. A schematic picture outlining the areas of hyperfluorescent neovascular membranes and subretinal blood was drawn of a projected 30 degrees fundus fluorescein angiogram. This drawing was superimposed on the size adjusted Octopus visual field. The changes in retinal sensitivity were calculated and related to angiographic changes. RESULTS: Three of the 19 patients had a reliability factor (RF) > 15% and were excluded from further analysis. In the remaining 16 patients the mean defect (MD) and loss variance (LV) values remained unchanged in patients showing regression of the choroidal neovascular membrane (CNVM) to irradiation at 12 months. MD was 7.7 (SD 1.7) at baseline and 7.6 (1.9) at 12 months (p = 0.86), and LV was 32.6 (13.9) at baseline and 32.4 (15.7) at 12 months (p = 0.94). However, in patients with progression of the CNVM at 12 months, both the MD and LV increased significantly during the 12 month follow up (MD from 7.3 (2.9) to 13.1 (3.6) (p = 0.05) and LV from 31.0 (22.9) to 71.8 (24.1) (p = 0.017)). When comparing the mean retinal sensitivity in the area of the primary CNVM (including classic, occult, and haemorrhagic components), the results were analogous: in patients with a regression of the CNVM after irradiation the mean sensitivity remained almost unchanged. It was 10.3 (6.4) dB at baseline and 9.4 (7.3) dB at 12 months (p = 0.58). In five out of 11 patients (45%) with regression of the CNVM, the mean retinal sensitivity even improved by 2.0-5.0 dB in the area of the original lesion during follow up. Instead, in patients showing progression of the CNVM at 12 months, there was a significant loss in mean retinal sensitivity--from 9.9 (4.6) dB at baseline to 1.0 (1.1) dB at 12 months (p = 0.019). The mean retinal sensitivity in the area of the irradiated but clinically normal retina during follow up was not significantly altered (21.5 dB at baseline, 19.7 dB at 12 months (p = 0.10)). CONCLUSIONS: Regression of subfoveal choroidal membranes in AMD after focal strontium irradiation is connected with stabilisation or even improvement of retinal sensitivity in central visual field measured by automated perimetry. Strontium plaque irradiation does not change the sensitivity in clinically normal paramacular retina during a 12 month follow up.

Aged↗
PubMed1982

Prospective comparison of the fluorescein-dilaurate test with the secretin-cholecystokinin test for pancreatic exocrine function.

In a prospective study of 60 patients undergoing investigation for possible exocrine pancreatic disease the fluorescein-dilaurate test was compared with the secretin-cholecystokinin (CCK) test. Forty one patients had a normal response to secretin-CCK, 14 patients had abnormal responses and in five patients the results were equivocal. Taking the secretin-CCK test as the diagnostic criterion, the fluorescein-dilaurate test had a sensitivity of 100% and a negative predictive value of 100%. There was a 54% false-positive rate. The fluorescein-dilaurate test is easy to perform and is a useful screening test for pancreatic exocrine insufficiency.

Adolescent↗
PubMedDate not supplied

Use of digitized fluorescein angiogram system to guide laser treatment of diabetic macular edema: a new technique.

We describe our technique to guide laser treatment in diabetic patients with clinically significant macular edema using a digitized fluorescein angiogram system. A pretreatment red-free photograph overlaid on a fluorescein angiogram is processed with the aid of the digitized fluorescein angiogram system in order to include all the important traces on it. The processed red-free image is used by the surgeon as a guide to the laser photocoagulation, improving treatment accuracy and safety for patients with diabetic macular edema.

Diabetic Retinopathy↗
PubMed1988

In vitro synthesis of fluorescein monoglucuronide.

A simple, economical method for the in vitro synthesis of milligram amounts of fluorescein monoglucuronide is presented. The compound can be synthesized in 1 day, and 2 chromatographic steps result in complete purification. The synthetic fluorescein monoglucuronide was found to be identical to biosynthetic fluorescein monoglucuronide by spectrophotometric and fluorometric criteria.

Animals↗
PubMed1995

Interaction of fluorescein derivatives with sulfonylurea binding in insulin-secreting cells.

Recently evidence was presented that fluorescein derivatives (e.g. phloxine B) inhibit glibenclamide binding by occupation of a nucleotide-binding site at the ATP-sensitive potassium channel (KATP channel). However, this conclusion was inconsistent with the results of previous studies testing the effects of nucleotides on glibenclamide binding. To elucidate the interaction mode of fluorescein derivatives with sulfonylurea binding, the effect of phloxine B on binding of [3H]glibenclamide to microsomes obtained from a pancreatic beta-cell line (HIT-T15) was examined. Phloxine B inhibited specific binding of glibenclamide half-maximally at 3.2 mumol/l. The slope parameter for the displacement curve was close to one, suggesting a competitive interaction between both drugs. In accordance with this assumption 4 mumol/l phloxine B did not show an effect on the number of high-affinity binding sites but increased the apparent dissociation constant for glibenclamide by 3.1-fold and 30 mumol/l phloxine B did not alter the rate of dissociation of [3H]glibenclamide. Moreover, MgATP (300 mumol/l) significantly reduced the apparent affinity for binding of phloxine B to the sulfonylurea receptor. This finding resembled the action of MgATP on binding of sulfonylureas to their receptor site. It is concluded that fluorescein derivatives inhibit glibenclamide binding due to competition for the same site at the sulfonylurea receptor.

Benzamides↗
PubMed1987

Fundus fluorescein angiography in generalized scleroderma.

Fundus affliction with generalized scleroderma was studied in 21 patients by ophthalmoscopy, fundus ocular photography and fluorescein angiography. Slitlamp examination of the anterior chamber, the iris and the lens revealed no evident affections. Neither did ophthalmoscopy reveal obvious abnormalities related to scleroderma. Abnormalities of pigmentation were not noted. Visual acuity was normal in 20 patients, and 1 patient had reduced visual acuity due to macular degeneration. Fundus fluorescein angiography was within normal physiological variation in 14, and definitely abnormal in 7 patients as assessed independently by 2 ophthalmologists. Angiographic abnormalities consisted of variable hyperfluorescence of the pigment epithelium layer, and, additionally, in 2 cases minute hyperfluorescence of the retinal layer. These angiographic abnormalities indicated affection of the retinal pigment epithelium probably caused by a vascular lesion of the choroidal layer. Retinal vessels were in general not affected. In conclusion, the choroidal vasculature appears affected in 1/3 of patients with generalized scleroderma as assessed by fundus fluorescein angiography.

Adult↗
PubMed1982

Experimental central serous chorioretinopathy in monkey eyes: fluorescein angiographic findings.

In an adult Japanese monkey (Macaca fuscatus), intravenous injections of adrenalin were repeated daily: 0.125 mg/kg for 7 days and 0.375 mg/kg from the 11th day on. In a cynomolgus monkey (Macaca irus), daily injections were carried out with intravenous adrenalin (0.11 mg/kg for 12 days and a double dose from the 13th day on) and also with intramuscular prednisolone. After the 39th injection in the former and after the 32nd injection in the latter monkey, disciform serous retinal detachment was seen to occur in the posterior pole region, and fluorescein angiography revealed multiple dye leakage spots at the level of the retinal pigment epithelium. Sometimes these changes subsided, but on continuing injections, these changes recurred on new locations. Two types of fluorescein leakage spots were recognized, i.e. ink blot type with progressive simple enlargement with time and the mushroom or jet type showing these patterns during enlargement. No abnormality was found in the optic disc, retinal vessels or in the choroidal circulation. The fluorescein angiographic findings were in close resemblance with those seen in the human central serous chorioretinopathy. It was discussed that these fundus changes of the monkey eye produced by repeated adrenalin injections would serve as a good animal model of the human disease.

Animals↗