Ultrastructural comparison of promastigote flagellates (leptomonads) of wild-caught Panamanian Phlebotomus.
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Base on intraperitoneal or subcutaneous inoculations, the tests of pathogenicity of T. vaginalis, for Mice, do not seem reliable, in our experiments. The impossibility of obtaining reproducible results is bound to the heterogeneity of reactions to parasite of Mice from the same strain. This individual effect demands a serious statistical study in the appreciation of pathogenicity.
A survey of enzyme variability between several strains of Trypanosoma cruzi allows to think that these organisms are diploids. According to the data sexuality, may be only occasional, has been hypothesized, at least in the recent past. The genetic distances between three strains are calculated for twelve loci.
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On a simple solid medium of indefinite content without hemin, colonies of Crithidia oncopelti were received. The clonal nature of them was proved. The survival in some experiments varied from 10 to 70%. Young colonies, 6 days after inoculation, were 0.3- 2.0 mm in diameter. C. oncopelti differed from Herpetomonas megaseliae and Crithidia harmosa in the morphology of colonies. To further genetical study of C. oncopelti, a possibility of cloning the object on a simple solid medium is an important prerequisite.
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