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[Simultaneous presence of a Cestode cysticercoid (Cyclophyllidae, Hymenolepididae) and a Trematode metacercaria (Dicrocoeliidae) in Oxydesmus granulosus Palisot de Beauvoir (Myriapoda, Diplopoda, Polydesmidae) from Dahomey].

We report observations of cysticercoids of Hymenolepis gilloni (Hunkeler, 1972), a flatworm parasite of five species of Crocidura in Africa. The cysticercoids are found up against the outside of the gut of Myriapode, Oxydesmus granulosus, from Porto Novo (Dahomey). We also found the metacercaria of a Trematode which is probably a Dicrocoeliidae.

Animals↗

[Bertiella deblocki, a new cestode Anoplocephalidae from a marsupial (Phalanger orientalis Storr) of East Timor].

Bertiella deblocki n. sp. (Anoplocephalidae: Anoplocephalinae) from the intestine of a marsupial Phalanger orientalis Storr of East-Timor differs essentially from Bertiella trichosuri Khalil, 1970, the most similar species, in the greater number of the proglottides, in the greater diameter of the scolex, in having a strongly strobilar musculature, the muscular outer edge of the genital atrium, the distal portion of the vagina surrounded by a muscular thickness, the uterus always crossing the excretory canals and in having the egg with two horns.

Animals↗

Genetic differences between Tunisian camel and sheep strains of the cestode Echinococcus granulosus revealed by SSCP.

Ovine and dromedary Echinococcus granulosus isolates from Tunisia were identified as G1 and G6 strains based on polymorphism of the mitochondrial cytochrome C oxydase CO1. Single strand conformation polymorphism (SSCP) was used in order to examine the genetic variation within and between Tunisian G1 and G6 strains and to estimate the extent of selfing. The dromedary isolates are genetically distinct from sheep isolates (high value of genetic variation between populations: Fst= 0.46). No significant deficiency in heterozygotes was found in sheep isolates, whereas heterozygote deficiency (suggesting selfing) was found in a limited number of camel isolates.

Animals↗

Energy dependence of protein synthesis by isolated cestode mitochondria.

Mitochondria of helminth parasites produce energy in a number of ways that vary from species to species, and, in general, do not possess a classical tricarboxylic acid (Krebs) cycle. The process of protein synthesis, which is energy dependent, is not well understood in this organelle. However, protein synthesis in helminth mitochondria seems to serve the purpose to the desired level despite its limited capacity to produce ATP compared to its mammalian counterpart. Data presented here demonstrate that helminth mitochondria can synthesize proteins in vitro and the process can be supported by energy supplied exogenously by the cytosol, by an ATP generating system utilizing a substrate such as malate, or by ATP itself.

Adenosine Diphosphate↗

Chemokinetic factors obtained from the larval stage of the cestode, Taenia taeniaeformis.

Saline extracts of the metacestodes of Taenia taeniaeformis were shown to have chemokinetic as well as chemotactic activity for equine polymorphonuclear leucocytes. Although parasite derived chemotactic activity could be appreciated at high protein inputs, such leucotactic activity was quickly lost upon dilution. In contrast chemokinetic activity was readily observed in saline extracts over a much broader range of protein inputs. Utilizing the Zigmond-Hirsch checkerboard assay the chemokinetic properties in the saline extracts were more pronounced when chemokinetic factors were loaded into the cell compartment of the chemotactic chambers only. Mild heat treatment or storage at 4 degrees C resulted in little destruction of the chemokinetic factors. However six cycles of rapid freezing and thawing generated marked increases in chemokinetic activity and not chemotactic activity. The results of this work provide for the first time evidence of parasite derived chemokinetic factors.

Animals↗

Expulsion of the gastrointestinal cestode, Hymenolepis diminuta by tolerant rats: evidence for mediation by a Th2 type immune enhanced goblet cell hyperplasia, increased mucin production and secretion.

The processes underlying expulsion of Hymenolepis diminuta in rats are not known. Expression levels of mRNAs of several cytokines revealed a Th2 response that differed between worm infection levels. IL-4 protein levels decreased while IL-13 levels increased in a 50-worm infection by 30 dpi; the converse was seen with a five-worm infection. A negative correlation was found between IL-4 or IL-13 mRNA expression and worm biomass, between IL-13 protein levels and worm number or worm biomass, and between IL-4 protein levels and worm biomass in 50-worm infections. A negative correlation between IL-4 mRNA or protein expression and worm biomass was observed with five-worm infections. A strong correlation between Muc2 mRNA expression and decreased worm number or biomass in a 50-worm infection was observed. Muc2 protein, goblet cell numbers and mucin decreased in a 50-worm infection by 20 days post-infection. These changes were not seen with five-worm infections where worms are not expelled. The data show that rats infected with 50 H. diminuta mount a Th2 response leading to high levels of IL-13, increased goblet cell numbers and increased mucin2 production and release. The mucus traps the worms, which are progressively expelled from the small intestine.

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