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PubMed1995

Strategy of biological monitoring and setting of biological threshold limits (BAT values) in Germany.

Biological monitoring is of fundamental importance in Germany. The successful strategy of biological monitoring is based on four factors. Recommendations for the pre-analytical phase have been issued by official societies. For the analyses in blood and urine specimens around 100 methods have been recommended and standardized. All measurements must be carried out under a quality control scheme issued by the German Ministry of Labor and organized by the German Society of Occupational and Environmental Medicine. The occupational medical judgement of the biological monitoring results take place by "Biological Tolerance Values for Working Materials" (BAT values) and "Exposure Equivalents for Carcinogenic Working Materials" (EKA values). A BAT value is, by definition, the maximum allowable concentration for an individual which should be exceeded; 53 BAT values for 37 agents or groups of agents and 18 EKA values for 10 compounds have been defined. These values have been published since 1982 in an annual list of occupational standards. Every BAT or EKA value is based on extensive scientific documentation.

Environmental Monitoring↗
PubMed1993

Combination-sensitive neurons in the primary auditory cortex of the mustached bat.

In the mustached bat, Pteronotus parnellii, neurons in the primary auditory cortex (AI) have been thought to respond primarily to single frequencies, as in other mammals. However, neurons in the Doppler-shifted constant-frequency (DSCF) area, a part of the mustached bat's AI that contains an overrepresentation of the prominent CF2 component of the biosonar signal, were found to show facilitative responses to combinations of different frequencies in the pulse and echo. The essential components for facilitation were the pulse FM1 and the echo CF2. The FM1-CF2 facilitation was sensitive to echo delays, indicating that DSCF neurons respond better to targets within particular ranges. On average, the longest discriminable echo delay, based on increased impulse counts due to facilitation, corresponded to a target range of 4.3 m, and the most discriminable delay corresponded to a target 3.6 m distant. Since mustached bats first show a behavioral response to a target at a distance of 3-4 m, DSCF neurons are suited to signal the presence of an insect within this behaviorally important range. DSCF neurons were broadly tuned to echo delay, with the average minimum discriminable echo delay corresponding to a target range of 1.9 m, and the delay tuning of the neurons followed (tracked) changes in pulse duration, indicating that facilitation occurs during much of the approach phase of insect pursuit when target characterization is presumably occurring. These results show that AI neurons in the mustached bat are specialized to respond to complex, behaviorally relevant stimuli during the search and approach phases of insect pursuit.

Acoustic Stimulation↗
PubMed1997

Spatially selective auditory responses in the superior colliculus of the echolocating bat.

When a bat approaches a target, it continuously modifies its echolocation sounds and relies on incoming echo information to shape the characteristics of its subsequent sonar cries. In addition, acoustic information about the azimuth and elevation of a sonar target elicits orienting movements of the head and pinnae toward the sound source. This requires a common sensorimotor interface, where echo information is used to guide motor behaviors. Using single-unit neurophysiological methods and free-field auditory stimulation, we present data on biologically relevant specializations in the superior colliculus (SC) of the bat for orientation by sonar. In the bat's SC, two classes of spatially tuned neurons are distinguished by their sensitivity to echoes. One population shows facilitated, delay-tuned responses to pairs of sounds, simulating sonar emissions and echoes. Delay tuning, related to encoding target range, may play a role in guiding motor responses in echolocation, because the bat adjusts its emissions with changes in target distance. The delay-facilitated response depends on the direction of stimulation and on the temporal relationship between the simulated emission and echo in the sound pair, suggesting that this class of neurons represents the location of a target in three dimensions. A second population encodes the target in two dimensions, azimuth and elevation, and does not show a facilitated response to echoes delivered from any locus. Encoding of azimuth and elevation may be important for directing head aim, and this class may function in transforming auditory spatial information into signals used to guide acoustic orientation.

Acoustic Stimulation↗
PubMed1998

Characterization of trypanosomes from the subgenus Schizotrypanum isolated from bats, Eptesicus sp. (Chiroptera: Vespertilionidae), captured in Florianópolis, Santa Catarina State, Brazil.

Seven Trypanosoma spp. isolates obtained from bats (Eptesicus sp.) were characterized using experimental infection in mice, triatomines, and culicines; complement lysis; indirect fluorescence assays; as well as isoenzyme and random-amplified polymorphic DNA (RAPD) profiles. The Trypanosoma sp. isolates were compared with Trypanosoma cruzi, Trypanosoma rangeli. and 2 other bat trypanosomes species, Trypanosoma vespertilionis and Trypanosoma hastatus. Trypanosoma sp. isolates were different from the other species in all experiments, except in complement lysis. Experimental infection of triatomines and culicines with Trypanosoma sp. proved to be transitory. These parasites were noninfective for both normal and immunosuppressed mice. Isoenzyme and RAPD profiles obtained for Trypanosoma sp. were quite distinct from T. cruzi and T. rangeli and closely related to T. vespertilionis and T. hastatus. No cross-reaction was observed between sera from mice infected with Trypanosoma sp. and the other trypanosomatids and vice-versa. Trypanosoma sp. induced no protection against T. cruzi infection in mice. The very low, or nonsimilarity between Trypanosoma sp. isolates and the other species used in this study suggests that they might be members of a distinct bat trypanosome species. However, further studies should be done to prove their affinities with Trypanosoma cruzi-marinkellei, another trypanosome species from bats.

Aedes↗
PubMed1998

Plasticity and patterns of evolution in mammalian salivary glands: comparative immunohistochemistry of lysozyme in bats.

Salivary gland plasticity was a significant adaptive feature in the mammalian radiation. This plasticity is reflected in remarkable and well-documented interspecific phenotypic variation in gland ultrastructure and in the chemical components of saliva. However, comparative data are still too sparse for determination of evolutionary trends that combine phenotypic patterns with evolutionary history and the actual secretory products. Although our theoretical approach assumes that natural selection has taken advantage of salivary gland plasticity in gene regulation, gland development, and secretory cell organelles and processes, it still is difficult to delineate the biological roles of secretory products in the context of ecological adaptation. In the present investigation we used immunohistochemical techniques and a polyclonal antiserum against lysozyme to compare the parotid and principal submandibular glands in a set of 12 species of microchiropteran bats. With this data set we used comparative methods and phylogenetic trees to develop the foundations for testable hypotheses about the molecular genetics and adaptive significance of lysozyme production in bats. By comparing immunohistochemical results with ultrastructure, lysozyme-like immunoreactivity was associated with serous secretory granules in parotid gland acinar calls, parotid gland intercalated duct cells, and submandibular gland demilune cells. Lysozyme production in submandibular gland demilune cells marks a point of evolutionary divergence between three families of insectivorous bats and four families composed of species with diverse diets (ranging from carnivory to nectarivory). In terms of diet, lysozyme-like immunoreactivity corresponded most strongly with feeding on hard-bodied insects, leading to the hypothesis that lysozyme serves as an important chitinase in bat saliva.

Adaptation, Physiological↗
PubMedDate not supplied

[Physiologic mechanisms of use of the Doppler effect in echolocation by Rhinolophus ferrumequinum bats].

During the flight, the greater horse-shoe bat R. ferrumequinum decreases the frequency in the medial part of the emmited signal in proportion to the relative target--bat velocity. The value of the decrease is approximately equal to the Doppler shift; as a result, the animal picks up the echo at its species specific frequency irrespectively of the flight velocity. Threshold curve of bat's auditory system plotted in terms of N4, exhibits a sharp turning peak with a slope of about 83 dB/kHz towards low frequencies. The species specific frequency lies between the frequencies corresponding to the highest and the lowest thresholds. The described peculiarities of emission and perception of signals allow to consider the orientation system in the bat as a Doppler compensating system which provides the effective detection and discrimination of moving targets (e.g., a prey), improves spatial characteristics of hearing and enables the animal to evaluate the velocity during approaching the target.

Animals↗
PubMed1997

Development of the organ of Corti in horseshoe bats: scanning and transmission electron microscopy.

The late prenatal and early postnatal development of the organ of Corti were studied in the horseshoe bat (Rhinolophus rouxi) by using scanning and transmission electron microscopy. Arrangements and dimensions of stereocilia bundles, together with their contacts with the tectorial membrane, were found to be adult-like shortly before birth, and thus before the biological onset of hearing (3-5 days after birth). During the first postnatal week, there were baso-apical gradients in disappearing kinocilia on inner hair cells (IHC), microvillis of supporting cells, and marginal pillars. The lower basal cochlear turn was mature with respect to these regressing structures at 3 days after birth, the apical turn at 10 days after birth. At birth, cytodifferentiation was found to be completed, and the tunnel of Corti and innermost spaces of Nuel had opened. The ultrastructure of IHCs was not markedly different from that at later ages. In outer hair cells (OHC), the adult-like regular arrangement of a single layer of subsurface cisternae and pillars was seen as soon as protrusions of supporting cells had withdrawn from the lateral wall of OHCs (basal turn at birth and throughout the cochlea 2 days after birth). Numerous efferent endings contacted the somata of IHCs up to the second postnatal week. Since the medial olivocochlear system is absent in horseshoe bats, the adult-like innervation pattern of OHCs was established at the biological onset of hearing. During the first 2 postnatal weeks, the cytoskeleton of pillar and Deiters cells, and the specialized Deiters cups developed. The organ of Corti appeared adult-like at 14 days, apart from the persistence of a reduced tympanic cover layer attached to the basilar membrane. Morphological data support physiological findings that the first broadly tuned auditory responses arise from the basal turn. The distinct low to high frequency gradient in development of sensitivity during the first 2 postnatal weeks of the horseshoe bat was not, however, matched by morphological gradients, and it would appear that the development of the cytoskeleton of supporting cells contributed to the establishment of tuning in the auditory fovea. Adult-like morphology of the organ of Corti coincided with the emergence of sharply tuned responses from the auditory fovea, but there was no clear-cut correlate for the shift in tuned foveal frequency representation that occurred during the following 3 weeks.

Animals↗
PubMed1996

Unusual aspects of inner cell mass formation, endoderm differentiation, Reichert's membrane development, and amniogenesis in the lesser bulldog bat, Noctilio albiventris.

BACKGROUND AND METHODS: The early embryogenesis of the lesser bulldog bat, Noctilio albiventris (family Noctilionidae), was examined histologically in 59 pregnant females collected from a reproductively synchronized population in Colombia. RESULTS: Early blastocysts of Noctilio are unusual in lacking a typical inner cell mass. Instead, cells inside of the trophoblast are dispersed for a period as a monolayer. A typical inner cell mass (ICM) only forms and becomes properly oriented after the initiation of implantation. Several features of Reichert's membrane in this species are also noteworthy: it develops between the ICM and trophoblast and between the parietal endoderm and trophoblast; it becomes linked to a meshwork of basal laminalike material that extends into the ICM; and it appears to be continuous, or fused, with prominent basal laminae that develop within the cytotrophoblastic villi that radiate throughout the preplacenta. Amniogenesis occurs by cavitation and converts the ICM into a hollow epiblastic vesicle. Gastrulation commences before this vesicle exhibits obvious differentiation into an embryonic shield and amniotic ectoderm. CONCLUSIONS: Because development and proper orientation of a typical ICM in Noctilio occur after the initiation of implantation, these may involve the migration of cells on the interior of the blastocyst and/or an unusual method of early endoderm differentiation. The possibility exists that epiblast, endoderm, and cytotrophoblast may all contribute to the secretion of Reichert's membrane in this bat. Although the early embryogenesis of Noctilio exhibits many similarities to that in phyllostomid bats, substantial differences also exist between these closely related species.

Amnion↗
PubMed1997

Megamitochondria in the serous acinar cells of the submandibular gland of the neotropical fruit bat, Artibeus obscurus.

BACKGROUND: As part of a continuing investigation of the comparative ultrastructure of chiropteran salivary glands, we examined the submandibular glands of eight species of neotropical fruit bats in the genus Artibeus. We previously described secretory granules of unusual substructure in the seromucous demilunar cells of this organ in some species in this genus. In the present study, we turned our attention to the serous acinar cells in the same glands. METHODS: Specimens of eight species of Artibeus were collected in neotropical localities. Salivary glands were extirpated in the field and thin slices were fixed by immersion in triple aldehyde-DMSO or in modified half-strength Karnovsky's fixative. Tissues were further processed for electron microscopy by conventional means. RESULTS: In contrast to seromucous cells, which exhibit species-specific diversification in bats of this genus, the secretory apparatus and secretory granules in the serous acinar cells are highly conserved across all seven species. The single exception involves the mitochondria in one species. In this instance, some of the serous cell mitochondria in Artibeus obscurus are modified into megamitochondria. Such organelles usually have short, peripheral cristae; a laminar inclusion is present in the matrix compartment of every outsized organelle. Inclusions of this nature never are present in normal-size mitochondria in the serous cells. None of the megamitochondria were observed in the process of degeneration. CONCLUSIONS: The giant mitochondria in A. obscurus have a matrical structure that is radically different from that of the only other megamitochondria reported to occur in bat salivary glands. The factors that lead to variation in megamitochondrial substructure in different species, as well as the functional capacities of such giant organelles, are unknown.

Animals↗
PubMed1997

Ultrastructure of the parotid salivary glands in seven species of fruit bats in the genus Artibeus.

BACKGROUND: In previous studies, we determined that the submandibular glands of five species of Neotropical fruit bats in the genus Artibeus had seromucous granules in their demilune cells with substructures that varied interspecifically in accordance with systematic relationships. Moreover, the striated ducts in these frugivores exhibited structural modifications that apparently are related to the consumption of a diet rich in potassium, but deficient in sodium. We now turn our attention to the parotid gland in a large number of species in this genus to determine if it follows the same structural pattern as does the submandibular gland. METHODS: Members of seven different species of Artibeus were live-trapped in various Neotropical locations. The parotid glands were extirpated from euthanized bats, fixed in the field, and prepared for electron microscopic examination by conventional means. The parotid glands in all seven species were virtually identical in morphology. The acinar cells (determined to be seromucous on the basis of ultrastructural criteria) contain large numbers of what appear to be vacuoles, but which are a type of secretory granule. These granules have an electron-lucent matrix and may contain one or several circular membranous profiles arranged either concentrically or in a random array. These granules appear to form by progressive dilatation of the termini of Golgi saccules, with the nascent granules finally severing their connection with the Golgi apparatus. Many of the internal membranous profiles are formed simply by invaginations of the limiting membrane of the granule; others may result from indentation of the limiting membrane by protrusions from adjacent granules; the source of multiple internal membranes in certain granules is unclear. The exocytosis of these granules results in the acinar and intercalated duct lumina being filled with an abundance of membranous material. Such extruded membranes are present in some striated ducts, but not in others, suggesting that they are degraded during passage through the duct system. The striated ducts are of conventional appearance, lacking the frondose processes that are prominent in the submandibular glands of Artibeus. CONCLUSIONS: The parotid gland in Artibeus shows none of the interspecific ultrastructural variability that characterizes the submandibular gland in bats of this genus. The seromucous acinar cells secrete granules that release phospholipids as well as glycoconjugates into the saliva. Based on the lack of frondose processes with their sodium-transporting portasomes, the striated ducts of the parotid gland are less concerned with electrolyte homeostasis than are those in the submandibular gland.

Animals↗
PubMed1997

Ultrastructure of the salivary glands in the midtongue of the common vampire bat, Desmodus rotundus.

BACKGROUND: All examined mammals have at least two sets of lingual salivary glands: von Ebner's glands and Weber's glands. A third set, the glands of Blandin and Nuhn, is present in the tongues of some but not all mammals. Vampire bats, Desmodus rotundus, are unusual in that they possess another set of lingual glands, these being in the midtongue region. METHODS: The anterior half of the tongue was extirpated from several adult vampire bats, dissected, and tissue blocks derived from the midregions of the body of the tongue prepared for transmission electron microscopy by conventional means. RESULTS: The midlingual glands are in the form of long, tubular secretory endpieces that are succeeded by ducts of simple morphology. In general, the secretory portions consist of two cell types, which may be intermingled in the same tubule or may form tubules that consist wholly of one cell type or the other. Seromucous cells usually have one or several rough endoplasmic reticulum cisternae that are hugely distended by a homogeneously dense material. Their granules have a bizonal substructure: one or several dense bands are embedded in a lighter matrix. Mucous cells are rather typical in structure, but their secretory product is different from run-of-the-mill mucous droplets. These droplets vary in density from cell to cell. In some cells, these droplets have a relatively light matrix; in other cells, the droplet is unusually dense, consisting mainly of a dark, structureless matrix with marginal lenticular lacunae of low density in which some short, irregular filaments are scattered. A rare finding is the presence of ciliated cells intermingled with secretory endpiece cells. The cilia are of conventional morphology. Secretory tubules are succeeded by ducts that resemble intercalated ducts; the epithelium of these ducts gradually increases in height to form a kind of excretory duct, without the intervention of striated ducts. As the ducts approach the lingual surface, the epithelium changes to stratified squamous. CONCLUSIONS: Saliva produced by the midlingual glands may be an aid in the reciprocal grooming behavior of vampire bats. Based on their morphology, the excurrent ducts may not modify the initial saliva elaborated by these glands and might act simply as pipelines by which the saliva reaches the mouth.

Animals↗
PubMed1997

A unique parotid gland in Hart's little fruit bat, Enchisthenes hartii.

BACKGROUND: Hart's little fruit bat, Enchisthenes hartii, is uncommon and, although it has been the subject of recent molecular genetic studies, is little known biologically. Because chiropteran salivary glands vary interspecifically in ways that reflect evolutionary history and ecology, we examined the parotid gland in E. hartii to ascertain the extent to which it resembles homologous glands in species to which this bat presumably is related. METHODS: The parotid glands were prepared for electron microscopic examination by conventional means. RESULTS: The parotid gland of E. hartii is structurally unique among all previously studied species of bats (> 230 species examined) and other mammals. In contrast to the same gland in other mammals, the parotid gland in E. hartii lacks secretory endpieces. In their place, there is a type of striated duct. Thus, in this species single secretory elements consist of (proceeding in the direction of salivary flow): striated duct--intercalated duct--and a conventionally located striated duct. The proximal ducts possess microvillus-lined intercellular canaliculi, whereas the walls of the distal ducts include occasional dark cells. Some small serous-like granules may be present in the intercalated duct cells. CONCLUSIONS: The function(s) and biological role of the unique parotid gland in E. hartii are unknown. Nevertheless, the presence of two sets of striated ducts provides two separate glandular components seemingly capable of electrolyte transport. This might be of adaptive significance in enabling this species to make use of tropical nutrient resources that otherwise would be unavailable. The uniqueness of its parotid glands lends support to the current hypothesis that E. hartii should be classified as a monotypic genus rather than as a species of Artibeus, whose members it resembles morphometrically.

Animals↗
PubMed1998

Ultrastructure of the binary parotid glands in the free-tailed bat, Tadarida thersites. II. Accessory parotid gland.

BACKGROUND: Many bat species have an extra set of major salivary glands. In some species, the accessory glands are quite similar to the principal one, but in others they may be radically different. Accessory glands usually are associated with the submandibular gland, but the free-tailed bat, Tadarida thersites, also has an accessory parotid gland. In the present study, we compared the accessory parotid gland with its principal counterpart. METHODS: Salivary glands were removed from two specimens of free-tailed bats, one of each sex, that had been live-trapped in western Kenya and immersion-fixed in a specially formulated mixture designed for field fixation. Once back in the laboratory, the tissue was further prepared for electron microscopy by conventional means. RESULTS: The secretory endpieces consist of serous tubules composed of typical serous cells that contain numerous dense granules. In contrast, the intralobular duct system shows a radical departure from normal. These ducts are enormous in caliber, their lumina measuring greater than 100 microm in diameter. They appear to arise by amalgamation of the homologues of intercalated and striated ducts into macroducts. The walls of the macroducts consist of intermingled patches of simple cuboidal and simple columnar epithelia that occasionally include a tuft cell and are underlaid by an almost continuous layer of myoepithelium. A few cells have some modified basal striations, but most cells display a cytological organization that differs radically from either of their two putative ancestral cell types. Both tall and short epithelial macroduct cells have a paranuclear collection of ovate mitochondria and aggregates of what presumably are peroxisomes. Macroduct cells in both the female and male are pervaded by a system of tubular smooth endoplasmic reticulum (SER). In the female, the SER gives rise to membranous whorls that consist of numerous plies. As the macroducts approach their termini, a single row of small dense secretory granules appears just beneath their luminal surface. At the lobular periphery, the ducts taper down to become excretory ducts of normal dimensions. CONCLUSIONS: An accessory parotid gland occurs in T. thersites, but apparently is absent in the related species, T. brasiliensis. The ultrastructural data are consistent with a possible steroidogenic function, although other features of the gland might relate to the elaboration of a secretory product associated with feeding on chitinous beetles. The macroducts conceivably function as reservoirs of preformed saliva.

Animals↗
PubMed2000

An extralemniscal component of the mustached bat inferior colliculus selective for direction and rate of linear frequency modulations.

Frequency modulations (FMs) are prevalent in human speech, and are important acoustic cues for the categorical discrimination of phonetic contrasts. For bats, FM sweeps are also important for communication and are often the only component in echolocation calls. Auditory neurons tuned to the direction and rate of FM might underlie the encoding of rapid frequency transitions. In the mustached bat, we have discovered a population of such FM selective cells in an area interposed between the central nucleus of the inferior colliculus (ICC) and the nuclei of the lateral lemniscus (NLL). We believe this area to be the ventral extent of the external nucleus of the inferior colliculus (ICXv). To describe FM selectivity of neurons in the ICXv and to compare it to other midbrain nuclei, up- and down-sweeping linear FM stimuli were presented at different modulation rates. Extracellular recordings were made from 171 single units in the ICC, ICXv, and NLL of 10 mustached bats. In the ICXv, there was a much higher degree of FM selectivity than in ICC or NLL and a consistent preference for upward over downward FM sweeps. Anterograde and retrograde transport was examined following focal injections of wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) into ICXv. The main targets of anterograde transport were the deep layers of the superior colliculus and the suprageniculate division of the medial geniculate body. The primary site of retrograde transport was the nucleus of the central acoustic tract in the brainstem. Thus, the ICXv appears to be a part of the central acoustic tract, an extralemniscal pathway linking the auditory brainstem directly to a multimodal nucleus of the thalamus.

Acoustic Stimulation↗
PubMed2000

Structure and function of the bat superior olivary complex.

The superior olivary complex (SOC) is a mammalian auditory brainstem structure that contains several nuclei. Some of them are part of the ascending system projecting to higher auditory centers, others belong to the descending system projecting to the cochlear nuclei or the cochlea itself. The main nuclei of the ascending system, the lateral and medial superior olive (LSO, MSO), as well as the lateral and medial nuclei of the trapezoid body (LNTB, MNTB), have been traditionally associated with sound localization. Here we review the results of recent studies on the main SOC nuclei in echolocating bats. These studies suggest that some SOC structures and functions are highly conserved across mammals (e.g., the LSO, which is associated with interaural intensity difference processing), while others are phylogenetically highly variable in both form and function (e.g., the MSO, traditionally associated with interaural time difference processing). For the MSO, these variations indicate that we should broaden our view regarding what functions the MSO might participate in, since its function in echolocation seems to lie in the context of pattern recognition rather than sound localization. Furthermore, across bat species, variations in the form and physiology of the MSO can be linked to specific behavioral adaptations associated with different echolocation strategies. Finally, the comparative approach, including auditory specialists such as bats, helps us to reach a more comprehensive view of the functional anatomy of auditory structures that are still poorly understood, like the nucleus of the central acoustic tract (NCAT).

Animals↗
PubMed1978

The basilar membrane of the bat, Pteronotus p. parnellii.

The basilar membrane of Pteronotus p. parnellii was studied by light and scanning electron microscopy in order to examine the relationship of membrane structure to the sharply tuned sense of hearing in this bat. The basilar membrane was found to differ from those of other mammals and other bats by showing virtually no change in width except at the extreme ends. Thickenings of the pars pectinata and pars tecta are well developed in Pteronotus; they show no sudden changes in their dimensions and in this way differ from the thickenings found in the European horseshoe bat whose sharply tuned sense of hearing seems at least partially dependent on sudden, marked changes in the structure of the basilar membrane. In Pteronotus the greater part of the basilar membrane, 7.5 mm or approximately 58%, lies within the enormous basal turn and within this turn there are steeply banked curves and one small 0.5-mm region where the membrane is straight. The straight portion is associated with a region of the cochlea where there is a marked change in the density of nerve fibers and where the stria vascularis, spiral ligament and fluid-filled spaces of the ear are enlarged.

Animals↗
PubMed1980

Structural remodelling of bat thyroid parafollicular (C) cells during development.

The development of bat parafollicular (C) cells was evaluated by electron microscopy from the time of early thyroid formation, through the neonatal period, to young adulthood. Parafollicular cells were relatively few in number (3%) at the earliest developmental stage examined, but increased with subsequent fetal stages, reaching a relatively stable level of 28% of parenchymal cell volume just prior to birth. Fetal parafollicular cells were packed with calcitonin granules. Following birth, however, parafollicular cells were totally or almost totally depleted of calcitonin granules. Moreover, another cell type that resembled the parafollicular cell was also found. This cell contained tiny granules which often contained two dense cores. Parafollicular cells of maturing, juvenile bats were characterized by the massive accumulation of secretory materal within the cisternae of the rough surfaced endoplasmic reticulum and a great deal of autophagy. Remarkably, none of these changes were found in young, adult bat parafollicular cells. In conclusion, this study provides morphological evidence to support the view that parafollicular cells play a role in developing mammals.

Animals↗
PubMed1984

Seasonal variations in pituitary LH-gonadotropes of the hibernating bat Myotis lucifugus lucifugus: an immunohistochemical study.

Pituitary gonadotropes were identified throughout the year in the seasonally breeding, hibernating bat Myotis lucifugus lucifugus by means of light microscopic immunohistochemistry. In both male and female bats, these cells were immunoreactive with an antiserum directed to the beta subunit of luteinizing hormone. Some gonadotropes were aggregated near a portion of the infundibular stalk which crosses the anterior lobe, while most were scattered singly in a uniform manner throughout the rest of the pars distalis. This cell population exhibited seasonal variations in both sexes. In males, the proportional volume of the pars distalis occupied by immunoreactive gonadotropes (volume fraction) was significantly reduced in late July, when plasma testosterone levels were approaching their seasonal peak. In females, the volume fraction declined in April, following ovulation, and remained low during pregnancy and lactation. The size and shape of gonadotropes appeared relatively constant throughout the annual reproductive cycle in male bats; the immunoreactive cells were irregular in shape, with cytoplasmic extensions insinuating between and often "cupping" other secretory cell types. In females, the gonadotropes resembled those of males throughout most of the year, except during pregnancy, when these cells became enlarged and ovoid. No evidence of involution was observed in these anterior pituitary cells in either males or females during hibernation.

Animals↗