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Clinical trial of prophylaxis of wound sepsis in elective colorectal surgery comparing ticarcillin with tinidazole. University of Melbourne Colorectal Group.

A prospective randomized single blind controlled clinical trial was undertaken to compare prophylactic therapy using a systemic antibiotic active against both aerobic and anaerobic bacteria with an oral antibiotic agent active only against anaerobic bacteria in elective colorectal surgery. One hundred and thirty-one patients received ticarcillin and 130 received tinidazole. The wound infection rate was 8% in those patients receiving ticarcillin prophylaxis and 20% in those receiving tinidazole (P less than 0.05). Multivariate analysis of the factors affecting wound infection rate showed that there were three independent factors that reached statistical significance: the prophylactic antibiotic used; the type of hospital (public or private) in which the operation was performed, and the presence of a stoma at operation. The wound infection rate in those patients receiving tinidazole prophylaxis was more than twice that reported previously by the authors. The mortality in patients receiving ticarcillin prophylaxis was 1.5% compared to 9.2% in those receiving tinidazole prophylaxis (P less than 0.05). The clinical anastomotic leakage rate was similar in each antibiotic prophylactic group, 8.6% in those receiving ticarcillin and 7.3% in those receiving tinidazole.

Administration, Oral↗

Antigenic characteristics of enterotoxigenic and nonenterotoxigenic isolates of Bacteroides fragilis.

Fecal isolates of enterotoxigenic (44 isolates) and nonenterotoxigenic (25 isolates) Bacteroides fragilis were obtained from diarrheic calves (62 isolates), lambs (2 isolates), and pigs (5 isolates). Using a Microtiter whole-cell agglutination test and gel double-diffusion analysis, the isolates were reacted with nonabsorbed rabbit antisera prepared against 13 isolates of enterotoxigenic B fragilis (ETBF). Isolates of B fragilis were antigenically diverse. Thirty-seven (84%) of the 44 isolates of ETBF comprised 13 serogroups on the basis of reaction in the agglutination test. Fourteen (56%) of the 25 isolates of non-ETBF comprised 4 of the 13 groups. Compared with results of the gel-diffusion test, most isolates had a different agglutination test reaction pattern against the 13 antisera. Isolates of ETBF could not be distinguished from non-ETBF. Antigenic heterogeneity of B fragilis facilitated differentiation of individual isolates, a capability that may be useful in future epidemiologic and virulence studies.

Agglutination Tests↗

Pathogenic anaerobic bacteria of man.

A range of anaerobic bacteria may be pathogenic to man. Those of particular clinical significance are reviewed and prospects for their control by antibiotic therapy discussed.

Bacteria, Anaerobic↗

Acute suppurative parotitis caused by anaerobic bacteria: report of two cases.

Staphylococcus aureus is the predominant pathogen in acute suppurative parotitis. Although anaerobic bacteria are prevalent in the normal oral and upper respiratory tract flora and frequently are involved in oral and facial infections, they have seldom been isolated from patients with suppurative parotitis. It may be that a role for anaerobes in parotitis has not been documented because of inadequate specimen transport or anaerobic culture techniques. We describe two cases of acute suppurative parotitis in which cultures yielded anaerobic bacteria. In one case, no aerobes were isolated; in the other, the anaerobe was the predominant organism numerically.

Acute Disease↗

[Septicemia, portal thrombosis and congenital protein C deficiency].

The authors report a case of Bacteroides fragilis septicemia with left portal vein thrombosis secondary to a liver abscess discovered by ultrasonography. This thrombosis was in relation with hereditary protein C deficiency. The authors emphasize the importance of assaying for C protein along with the other physiological coagulation inhibitors in the child presenting with venous thrombosis.

Bacteroides Infections↗

Perinatal septicemia due to the Bacteroidaceae.

Two cases of perinatal septicemia due to the Bacteroidaceae are discussed. In contrast to most cases of perinatal septicemia caused by aerobic bacteria, these cases were not associated with prolonged rupture of the fetal membranes.

Adolescent↗

Acute appendicitis and Bacteroides fragilis.

Bacteria belonging to the Bacteroides fragilis group (B. fragilis, B. ovatus, B. vulgatus, B. distasonis, B. thetaiotaomicron and B. uniformis) were cultured in quantities of less than or equal to 10(5) c.f.u./ml from the excised appendix from 30 patients. Twenty-two patients (group I) had an acute purulent appendicitis (three with perforation), four were classified as slightly inflamed cases (group 2) and four had a normal appendix (group 3). The B. fragilis group of bacteria dominated among the anaerobic isolates, but Bifidobacteria, Fusobacteri, Clostridium perfringens, Lactobacilli, Leptotrichia and Veillonella, in decreasing order, were isolated as well. Among isolates of aerobic bacteria, E. coli was most frequently isolated (26/30 patients). No other genus was isolated from more than four of the appendices. Serological investigations of the humoral antibody response to polysaccharide antigens from four of the Bacteroides species showed that a doubling of the titer, or more, in paired serum samples could be observed as follows: against B. fragilis 6/22 patients (group 1), 3/8 patients (groups 2 and 3). The corresponding figures for B. ovatus were 11/22 and 2/8, B. vulgatus 3/22 and 0/8 and B. distasonis 4/22 and 0/8. Sera from two patients with ruptured appendix, and B. fragilis cultivated, showed the highest titer increases against the B. fragilis antigen. The data from the bacteriological and immunological investigations make us conclude that (i) the B. fragilis group of bacteria is the most common anaerobic group of bacteria isolated from normal and diseased appendices, (ii) the mere isolation of B. fragilis, encapsulated or not, does not imply an etiological role in appendicitis since there was no obvious difference in titer increases against B. fragilis in sera from patients with or without an inflamed appendix, and (iii) high, and significant, titer increases against B. fragilis are seen in sera from patients with ruptured appendix.

Acute Disease↗