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[Ultrastructural study of the biodegradation processes. I. Beech (Fagus sylvaticus L.) leaf white rot].

An electron microscopic study of beech leaf white rot shows a certain number of characteristic developmental stages which are identical whether the material is from in vitro experimentation or from natural incubation. Endowed with a cellulolytic properly seemingly localized in the apical region only, hyphae of the white rot agent only traverse the plant cell walls. Subsequently, hyphae penetrate condensed protoplasmic residues and make them progressively transparent to electrons. During this discoloring process, a lethal factor of as yet unknown nature appears, affecting other microorganisms already present in the leaves. Phloem and xylem vascular bundles do not present notable ultrastructural modifications. Therefore, leaf discoloration is not due to an alteration of the xylem constituents but to changes having occurred in the condensed cytoplasmic residues of the dead tissues.

Bacteria↗

[The vessels of the retropancreatic space in grey-scale-ultrasound. A contribution to the topography of the pancreas (author's transl)].

Normal pancreatic parenchyma and retropancreatic vessels can be well visualized and differentiated by grey-scale-tomography. The posterior border of the head and body of the pancreas can be defined better with the grey-scale-technique than with other ultra-sound-techniques by visualization of the retropancreatic vessels. For this purpose the venous system (portal vein, splenic vein and superior mesenteric vein) is more important than the arteries because of their closer topographic relationship to head and body of the pancreas. Identification and separation of those veins from other vascular structures is easily possible because of their constantly reliable topography and typical configuration on transverse section. The spleno-porto-vascular bundle--resembling a tadpole--should be demonstrated well enough to avoid the occasional confusion with the pancreas itself.

Humans↗

The use of free full thickness skin grafts in the treatment of complications of burns.

The main problem in the treatment of complications of deep burns is to cover the defects with skin of good quality. Although many regions can be covered by split skin grafts, it is important that some areas are covered with full thickness skin and subcutaneous fat in order to get an adequate cosmetic and functional result. In these cases rotation flaps or tubed pedical flaps have been frequently used in the past. Tubed pedical flaps are difficult to construct and have many disadvantages. With the aid of microsurgery it is possible to isolate free skin flaps with the vascular bundle supplying the flap with blood. These flaps can be completely removed from the donor area in one operation and can be placed onto the recipient area. After the vessels of the free flap have been anastomosed to the vessels in the recipient area, the function of the transplanted skin will remain normal. The colour of the transplant, its elasticity and its consistency and preserved. These one-stage techniques greatly facilitate the reconstruction after deep burns. In addition, the quality of the skin varies according to the site of the skin flap taken and it is thus possible to chose the type of skin most appropriate to the recipient zone. The authors describe the different types of free skin flaps, mentioning their qualities and their disadvantages. Finally, they discuss which skin flaps are most useful to cover particular skin defects.

Axilla↗

[Avoiding circular scars on the extremities].

Circular scarring on extremities during replantation, pollicization, and "on-top"-plasty could strangulate nerves, vessels and tendons beneath the scar. Decompression could be achieved by single or multiple Z-plasty. During replantations we use the incisions for the Z-plasty to dissect the ends of the nerves and vessels for suturing. The scar created has a zig-zag form which with time becomes more flat but does not cause constriction of neuro-vascular bundles and tendons. The method is simple and does not take too much time. It may produce a better functional result.

Cicatrix↗

[Zonal anatomy of the prostate using endorectal MRI].

The development of an endorectal surface coil now permits a partial study of the anatomical model developed by McNeal. Axial and coronal views, which were used to establish the model can be obtained in a short period of time with fast spin echo sequences. Axial views are performed along the proximal urethra and coronal views are performed along the axis of the distal urethra and the ejaculatory ducts. Anatomical boundaries of the transitional zone are well delineated on axial views, illustrating the concept of "inner gland". The prostatic capsule and the neuro-vascular bundles, pathways of extension of the cancer out of the prostate are also well delineated. Coronal sections allow a very good anatomical study of the caudal junction of the vas deferens and the seminal vesicles (the so called weak space), pathway of tumor extension to the seminal vesicles. Differences in signal of the prostatic zones make the outer gland cancers very conspicuous as well as some transitional cancers which can show, in some cases, an homogeneous hyposignal.

Humans↗

Andrological laparoscopy.

The laparoscopic technique has well defined indications for some andrological procedures such as the diagnosis and the treatment of cryptorchidism, but its role remains controversial in varix ligation for which laparoscopy is however the newest development. At the Institute of Urology of the University of Milan from January 1992 to June 1994 five adults patients with undescended and unpalpable testis underwent laparoscopy. In 4 cases laparoscopic orchiopexy (2 direct and 2 staged procedures) and in one case laparoscopic orchiectomy have been performed. In the same period 20 cases of varicocele (6 bilateral) have been observed and treated by laparoscopic varix ligation. For cryptorchidism after the identification of the testis we decide on the basis of parenchimal trophism wheter to perform orchidopexy or orchiectomy. A single step laparoscopic orchiopexy can be performed if the undescended testis is located proximal to the internal inguinal ring and if the mobilization of the spermatic vessels allows it. A Fowler-Stephens staged orchiopexy is indicated for intra abdominal testicle with short spermatic vessels. In the first stage the spermatic vessels are isolated and divided relying on the compensation offered by the deferential and extrafunicular vessels. After six months, once the testis trophism has been ascertained, the testis can be placed in the scrotum. For varicocelectomy the peritoneum is incised at the projection of the spermatic cord from the internal inguinal ring. A blunt and gentle dissection prepares the spermatic vascular bundle, the spermatic artery is identified and isolated and the vein are clipped and divided.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

[Contents of cyclic nucleotides and activity of lysosomal enzymes in heart, brain and liver tissues in the early postresuscitation period].

Levels of cyclic nucleotides (CN) in cardiac, cerebral, and hepatic tissues were measured in the early postresuscitation period in rats exposed to systemic circulation arrest induced by clamping of the cardiac vascular bundle. Changes of CN levels in the examined tissues were different in principle. In the heart cAMP levels progressively reduced 30 and 60 min after resuscitation, whereas cGMP levels were increasing. On the contrary, in the liver cAMP levels increased and cGMP content reliably reduced. Levels of cAMP and cGMP reliably reduced in the basis cerebri, with a trend to their reduction observed in the hemispheric cortex. Lysosomal hydrolases were activated in the said parts of the brain, their fraction was isolated in percoll gradient 30 min after resuscitation. In lysosomal fraction isolated from cardiac and hepatic tissues at the same period no activation of acid phosphatase and cathepsin D was observed in comparison with the control. It may be assumed that activation of lysosomal enzymes is governed by CN deficit.

Acid Phosphatase↗

[Prenatal diagnosis of a placenta praevia percreta with color Doppler. A case report. Review of the literature].

Placenta praevia percreta, with bladder invasion, was diagnosed at 29 weeks of amenorrhoea with colour Doppler which visualized vascular bundles leaving the placenta and reaching the lower part of the bladder. These bundles were identified as including arterial elements with pulsed Doppler. The criteria for the diagnosis of placenta accreta with ultrasonography and colour Doppler have been presented in the literature. This prenatal diagnosis allowed adapted preoperative management and intensive care, however air embolism could not be avoided and the patient died at the end of the operation.

Adult↗

Post irradiation sarcoma of soft tissue and bone.

Thirty-eight patients with sarcomas of soft tissue and bone which followed previous radiotherapy have been treated at the Royal Marsden Hospital between 1951 and 1992. The commonest reasons for initial radiotherapy were carcinoma of the breast and lymphoma, and the median time to development of sarcoma was 11 years. The only long term survivors were those who had complete surgical excision. Seven patients required forequarter amputation (30% of the extremity sarcomas) which reflects their frequent situation in the shoulder girdle and upper arm and the close proximity to the brachial plexus and vascular bundle. The overall prognosis was poor (5-year survival 30%) and probably reflects the poor prognostic factors (size, grade and tumour site) present at the time of diagnosis.

Adolescent↗

Revascularization procedures in the treatment of Kienböck's disease.

Lunate revascularization by means of vascular bundle implantation is one of the useful procedures for stages I and II Kienböck's disease, but for stage III, it should be combined with some biomechanical procedure or partial carpal fusion. There is no indication for this procedure in stage IV disease.

Adolescent↗

[Clinical use of the C-shaped cross finger flap].

The C-shaped cross finger flap is an axial flap based on the digital vascular bundle either distally or proximally. We have successfully used this flap to repair different defects of the finger in 12 cases. The flap can be made rather big and the operation is easy. The surgical technique and the advantages and disadvantages are described.

Adolescent↗

[Transtrochanteric rotation osteotomies for osteonecrosis of the femoral head. Apropos of 20 cases].

PURPOSE OF THE STUDY: Twenty consecutive rotation osteotomies for idiopatic necrosis of the femoral head were reviewed with an average follow-up of 6,5 years, in order to evaluate an original technique (which uses a nail plate for rotation and fixation of the fragments), and to determine the middle term results (and therefore indications) of anterior and posterior rotation osteotomies. MATERIALS AND METHODS TECHNIQUE: rotations were achieved by rotating the femoral head with the nail of the nail plate, and without dissection of the posterior vascular bundle. We performed 16 anterior rotation osteotomies (according to Sugioka, with an average rotation of 52 degrees) and 4 posterior rotation osteotomies (described by Kempf, with an average rotation of 77 degrees). Only two patients were lost after 2 years follow-up (with good result), and the radio-clinical outcome of 18 operations at 5 years was known. RESULTS: Global results were : 7 failures, 3 fair and 10 very good or good. In the 4 posterior rotations (Kempf) we achieved 4 very good results, even in Ficats stage 3. In the 16 anterior rotations we could in all cases obtain, on the hip in extension, an almost complete discharge of the necrotic zone, as after osteotomy it was no more in front of the acetabular major bearing zone (defined as an angle of 40 degrees around the apex of the femoral head on the lateral Lequesne view). We obtained 6 good and very good results, 3 fair, and 7 failures requiring a THR. There were 2 factors of poor prognosis : Stage of the necrosis, as we observed 4 failures in the 4 Ficat's stage 3, and only 3 failures in the 12 stage 2. Depth of the necrosis, as we achieved 6 very good and good results and 1 poor in the 7 cases when it was no more than 1/3 of the head diameter. But in the 9 cases where depth was over one third there were 3 fair and 6 poor results. DISCUSSION: Our technique proved to be reliable as it achieved the rotation planned before operation (only one hypo-correction of 15 degrees) and bone fusion, allowing full weight bearing at 3 months in all cases. No extension of the necrotic area was observed. Posterior rotation osteotomy was followed by long term favorable results, may be because it achieves an anatomic discharge of the necrotic zone not only when the hip is in extension, but also when the hip is flexed. Anterior rotation is only recommended when : a rotation not exceeding 60 degrees (therefore without risks for the posterior bundle) allows a discharge of the necrotic zone when the hip is in extension. The necrosis is stage 2. In Stage 3 a progressive arthritis may occur as, in hip flexion, the necrotic sector of the non spherical head comes in front of the acetabular major bearing zone. The depth of the necrosis does not exceed 1/3 of the femoral head, such as in cases of a very large necrosis, mechanical degradation of the non necrotic part of the head may occur, even if discharge of the necrosis is achieved. CONCLUSION: Transtrochanteric rotation osteotomy may delay of a decade or more the occurring of osteoarthritis, if its indications are restricted to patients under 40, suffering from idiopatic necrosis. In our series Sugioka osteotomy gave good results in stage 2 when necrosis depth was no more than 1/3 of the head diameter. Posterior osteotomy allows a better discharge of the necrotic zone and thus may be proposed in less restricted conditions.

Adult↗

[The patterns of focal reorganization of the synaptic pool at the molecular level of the cerebellar cortex in the ischemic period].

A comparative analysis of intraneuronal relationship between Purkinje cells and granule cells zones of the cerebellar cortex molecular layer with different extent of expression of destructive and compensatory-adaptive processes was performed in rats in the dynamics of postischemic period. The ischemia was modelled by the 10 minutes long compression of the heart vascular bundle under the ethereal narcosis, Dependence between type and extent of the synaptic profile and functional state of the synapse was shown. Dynamics of changes of the synapse numerical density was demonstrated so as the regularities of the axo-spinous synapses structural organization changes during their dysfunction, destruction and interneuronal contact repair within and outside the nidi of interneuronal interrelations during 30 days of postischemic period.

Animals↗

[Madelung's cervicodorsal adipose hypertrophy (the Launois-Bensaude syndrome)].

Based on an experience (that we believe may be very low in any Clinic) of three cases of cervicodorsal fatty hypertrophy (Launois-Bensaude syndrome) in one case with relapse of the disease, we performed complex anterior dissection of the region, with access of both vascular bundles of the neck and with subsequent surgical removal of the left hypertrophic maxillary salivary gland, followed in a second step (3 months after the primary operation) by the dissection of the dorsal fatty tumours. The aesthetic and functional results were excellent. In the postoperative step, the patient follows a radiation therapy at a dose for anti-inflammatory purpose and steroid therapy.

Adult↗

[Anterior tenoarthrolysis in flexion contracture of the fingers. Apropos of 41 cases].

PURPOSE OF THE STUDY: The "hook finger" with PIP and DIP flexion deformity, after multiple previous operations is difficult to treat. Classical PIP arthrolysis combined with tenolysis are frequently unsuccessful. We studied the result of an operation of Total Anterior Teno-Arthrolysis (TATA) as described by Saffar in 1978. MATERIALS AND METHODS: Among 41 patients with a mean age of 33 years, 73 per cent had at least one previous operation, on the flexor apparatus in the majority of cases. Global PIP and DIP lack of extension was 131 degrees with a mean PIP lack of 80 degrees. The operation consists in "en bloc" lifting of the finger volar aspect including the two neuro-vascular bundles, the flexor apparatus and the two volar plate, by dissecting underneath the periosteum of phalanges palmar aspect. The denuded distal squeleton is best covered by DIP arthrodesis combined with shortening. RESULTS: Only one complication occurred with PIP septic arthritis. With a mean follow up of 7.6 years, four fingers were not improved, none was worsened and 37 were improved. Mean post-operative PIP extension lag was 30 degrees, giving a global (PIP and DIP) gain of 86 degrees. The PIP AROM was only improved of 38 degrees. CONCLUSION: The benefit of this salvage operation is mainly a change of the AROM in a more functional arc. This has to be clearly explained to the patient and if accepted the satisfaction is high (90 per cent in our series).

Adolescent↗

The dorsal branch of the digital nerve: an anatomic study and clinical applications.

The dorsal branch of the digital nerve was studied in 40 fingers from 8 cadavers. Our anatomic dissections showed that the dorsal branch of all digital nerves was constantly present. It arose from the digital nerve at the base of the proximal phalanx where digital vascular bifurcation usually occurred. The dorsal branch crossed the digital vascular bundle posteriorly and lay just above the extensor mechanism deeper to the dorsal-sensitive branches of the ulnar and radial nerves in the proximal and middle part of the proximal phalanx. The dorsal branch of the digital nerve supplies not only the dorsum of the middle phalanx, but also sends a branch to the dorsum of the proximal phalanx. Both dorsal branches should be used for maximal sensation while performing an innervated cross-finger flap. Furthermore, if only one digital nerve anastomosis is performed, a painful neuroma may develop from the unrepaired digital nerve stump. Therefore both of the transected digital nerves should be repaired. The dissections of the dorsal branches may be started proximally from distal palmar crease when any difficulties are encountered for nerve isolation. Nerve stumps of the dorsal branches should be placed in a deeper tissue plane and epineural sutures may be useful to prevent painful neuroma. This technique should be used in selected patients to gain maximal sensibility regarding the described points.

Adult↗

[Contribution to the problem of optic nerve excavation (author's transl)].

To study opinions on the formation of optic nerve head excavation we had the opportunity of examining microscopic sections of the papilla of a 10-month-old baby who showed bilateral considerable border excavation without the presence of hydrophthalmus or increased intraocular pressure. The thickened lamina cribrosa showed centrally no perforations so that the nerve fibres had to avoid the unperforated parts of the lamina. According to the size and position of such unperforated laminar areas, types of excavation with various defects on the optic nerve head resulted. By coincidence of both components in the optic nerve stem (increased connective tissue growth, and formation of a small vascular bundle), considerable collections of mesenchyme could occur which then resulted in a spreading apart of the optic nerve fibres. As long as neuroglia formation has not filled the gaps in the nervous tissue originally, a secondary growth of glial tissue can follow an original connective tissue-caused defect formation. Hence the deposits of glia in the excavated optic nerve head would be not the cause, but rather the effect of defect formation.

Abnormalities, Multiple↗

[Effect of temporary ischemia with subsequent recirculation on the ultrastructure of glomerular capillaries and nephrons].

The influence of temporary ischemia followed by recirculation on the ultrastructure of glomerular capillaries and some portions of the nephron was studied in 20 albino rats which were subjected to compression of the vascular bundle of the left kidney for 30 min., 1, 2 and 3 hours followed by recovery of the blood stream in the ischemic organ for 3 hours (with a simultaneous nephrectomy of the right kidney). The electron microscopic analysis has established that the amount of micropinocytic vesicles become markedly increased within 3 hours following 30-min. ischemia of the kidney with the recovery of blood circulation in the cytoplasm of endotheliocytes of glomerular blood capillaries. With increased terms of the left kidney ischemia (1, 2, 3 hs) the ultrastructural changes in endotheliocytes increased. There appeared microclasmatosis of the internal plasmalemma of endotheliocytes, the flattened peripheral part of the cytoplasm underwent considerable destruction. Swelling of microvilli of the brush border and vacuolization of the cytoplasm were observed in nephrocytes of the proximal part of the nephron in short-term ischemia (30 min) followed by the recovery of circulation for 3 hours. Longer periods of ischemia (1, 2, 3 hs) casued destruction of the brush border. There appeared secondary lysosomes, in mitochondria there occurred discomplexation and lysis of cristae.

Acute Disease↗