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PubMed2003

Nested reciprocities: the organism-environment system in perception-action and development.

The ecological approach to studying perception and action is one in which researchers view the structure in ambient energy arrays surrounding a given organism as being sufficient to specify meaningful environmental properties to that organism. Thus, from this perspective, perception is viewed not as a cognitive achievement but as a lawful state of affairs that exists in an organism-environment system. The approach to studying development known as transactionalism is one in which researchers view the developmental process as the expression of a complex web of endogenous and exogenous factors. Thus, from this perspective, development is not an unfolding of a genetic program, but rather an ongoing exchange among levels of the organism-environment system that continues throughout the life span of the organism. Despite their independent development, these two meta-theoretical approaches have much in common. In each approach, the organism-environment system is the fundamental unit of analysis, and the development of perception and behavior is spread across the multiple levels of this system.

Animals↗
PubMed1994

Directed protein replacement in recombination full sites reveals trans-horizontal DNA cleavage by Flp recombinase.

One round of site-specific recombination between two DNA partners mediated by the Flp recombinase requires the breakage and reformation of four phosphodiester bonds. The reaction is accomplished by the combined action of four Flp monomers. Within the recombination complex, what is the relative disposition of a Flp monomer with respect to the target diester that it cleaves? To address this question, we have devised a strategy for the targeted orientation of Flp monomers within full-site recombination substrates. Our experimental design is not dependent on 'altered binding specificity' of the recombinase. Analysis of the pattern of DNA cleavage by this method reveals no evidence for DNA cleavage in cis. A Flp monomer bound to its recognition element within the full site does not cleave the scissile phosphodiester bond adjacent to it. Our results are most consistent with 'trans-horizontal cleavage'. Cleavage by Flp occurs at the scissile phosphodiester distal to it, but within the same full site. The general experimental design employed here will be of widespread utility in mechanistic analyses of nucleic acid transactions involving multimeric DNA-protein assemblies.

Base Sequence↗
PubMed2004

Characterization of human ribosomal protein S3 binding to 7,8-dihydro-8-oxoguanine and abasic sites by surface plasmon resonance.

The human ribosomal protein S3 (hS3) possesses multifunctional activities that are involved in both protein translation, as well as the ability of cleaving apurinic/apyrimidinic (AP) DNA via a beta-elimination reaction. We recently showed that hS3 also has a surprising binding affinity for an 7,8-dihydro-8-oxoguanine (8-oxoG) residue embedded in a 5' end labeled 37mer DNA oligonucleotide. To understand the interaction of hS3 and DNA templates containing 8-oxoG, we carried out real-time analysis using surface plasmon resonance (SPR). Notably, hS3 was found to have an apparent three orders of magnitude higher binding affinity (KD) for 8-oxoG than the human N-glycosylase/AP lyase base excision repair (BER) enzyme OGG1. An even more dramatic five orders of magnitude higher binding affinity for AP DNA was found for hS3 as opposed to hOGG1. These results suggest that ribosomal protein hS3 may have a multifunctional role that may also affect functions associated with DNA base excision repair transactions.

Apurinic Acid↗
PubMed2004

Complex social behaviour can select for variability in visual features: a case study in Polistes wasps.

The ability to recognize individuals is common in animals; however, we know little about why the phenotypic variability necessary for individual recognition has evolved in some animals but not others. One possibility is that natural selection favours variability in some social contexts but not in others. Polistes fuscatus wasps have variable facial and abdominal markings used for individual recognition within their complex societies. Here, I explore whether social behaviour can select for variability by examining the relationship between social behaviour and variability in visual features (marking variability) across social wasp taxa. Analysis using a concentrated changes test demonstrates that marking variability is significantly associated with nesting strategy. Species with flexible nest-founding strategies have highly variable markings, whereas species without flexible nest-founding strategies have low marking variability. These results suggest that: (i) individual recognition may be widespread in the social wasps, and (ii) natural selection may play a role in the origin and maintenance of the variable distinctive markings. Theoretical and empirical evidence suggests that species with flexible nesting strategies have reproductive transactions, a type of complex social behaviour predicted to require individual recognition. Therefore, the reproductive transactions of flexible species may select for highly variable individuals who are easy to identify as individuals. Further, selection for distinctiveness may provide an alternative explanation for the evolution of phenotypic diversity.

Animals↗
PubMed1988

Mergers and acquisitions: new arrangements in health care. Part 1.

Mergers and acquisitions are assuming a more important role in the healthcare industry today. These transactions require various issues be considered, such as valuation, capital planning, and so forth. In this article, the first in a five-part series on mergers and acquisitions, the fundamental methods and techniques of valuation are discussed. Some of these valuation methods, including comparative market transactions and free cash flow, are explained and examples are used to help potential purchasers and sellers to determine an organization's true value. Other articles in this series will include legal issues, tax implications, purchase investigations, and capital planning for mergers and acquisitions.

Accounts Payable and Receivable↗
PubMed1993

Property rights and payment to patients for cell lines derived from human tissues: an economic analysis.

The increasing potential for commercial applications in biotechnology has given rise to new legal and ethical questions with regard to ownership of human tissue. As the potential value of human cells and tissue has risen, so have donors' calls for a share in the profits. However, in a recent California ruling (John Moore vs the Regents of the University of California), the court once again held to its traditional position that individuals do not hold property rights in their own tissue and cells. We will show that, in the rare case where tissue value may be determined prospectively, a one-time payment (and, hence granting a property right) is efficient. Moore is such a case. In general, however, the transactions costs of granting full property rights to donors of tissue and cells outweigh the benefits of such a change in policy.

Biomedical Research↗
PubMed2005

Specific interference between two unrelated internal ribosome entry site elements impairs translation efficiency.

Internal ribosome entry site (IRES) elements allow simultaneous synthesis of multiple proteins in eukaryotic cells. Here, two unrelated IRESs that perform efficiently in bicistronic constructs, the picornavirus foot-and-mouth disease virus (FMDV) and the cellular immunoglobulin heavy chain binding protein (BiP) IRES, were used to generate a tricistronic vector. Functional analysis of the tricistronic RNA evidenced that the efficiency of protein synthesis under the control of BiP IRES was lower than that of the FMDV IRES, relative to the efficiency measured in bicistronic vectors. A specific competition between these elements was verified using two separate mono- or bicistronic constructs in vivo and in vitro. In contrast, no interference was detected with the hepatitis C virus (HCV) IRES. The interference effect of FMDV IRES was observed in cis and trans, in support of competition for common transacting factors different than those used in cap- and HCV-dependent initiation.

Animals↗
PubMed1998

Addiction recovery for low-income pregnant and parenting women: a process of becoming.

A naturalistic field study of low-income women (N = 17) in an intensive outpatient addiction recovery program addressed the question: What is the nature of addiction recovery for pregnant and parenting-women in an addiction treatment program? Grounded theory methodology was used to determine the nature of the interpersonal and social processes that define addiction recovery for women in this study. Over 2 years, audiotaped semi-structured interviews, document reviews of medical records, treatment progress and group therapy notes, and participant observation notes were collected and analyzed. The constant comparison method of analysis involved an ongoing process of theoretical sampling, memoing, and open and then axial coding to identify, group, link, and reduce the categories produced. A developmental model of addiction recovery in pregnant and parenting women emerged that consisted of the dimensions of becoming drug and alcohol free, a partner in a relationship, a person, and a parent. These four dimensions parallel and transform each other, yielding different outcomes but similar patterns over time. This model of addiction recovery provides a beginning framework for understanding the transactional nature of addiction recovery for low-income women who are adapting to a drug and alcohol-free lifestyle and the task and role of parenting a newborn.

Female↗
PubMed2001

Dissonant imperatives in nursing: a conceptualization of stress in intensive care in Sweden.

The aim of this study was to explore nurses' experiences of stress within the context of intensive care. The theoretical perspective for the study builds on a cognitive-phenomenological-transactional theory of stress and coping and the theory of cognitive dissonance. Respondents were 36 registered nurses recruited from 10 intensive care units (general, neonatal and thoracic units). Their experience as nurses ranged from one to 32 years. These intensive care units had similar structural characteristics, namely a high working pace, advanced technology, constrained finances, frequent reorganizations, a shortage of registered nurses and all were filled to overcapacity. Data were collected in open-ended interviews that were audio-taped and transcribed. A content analysis identified four contradictory themes: (1) controlled by the work situation--needing to be in control; (2) constrained by prioritization--wanting to do more; (3) lacking the authority to act--knowing that something should be done; and (4) professional distance--interpersonal involvement. These four themes were synthesized at a higher level of abstraction into a main theme: stress induced by dissonant imperatives, which conceptualizes nursing stress in the intensive care unit. In conclusion, dissonant imperatives might lead to stress in intensive care nursing.

Humans↗
PubMed1991

Identification of NolR, a negative transacting factor controlling the nod regulon in Rhizobium meliloti.

In Rhizobium meliloti, expression of the nodulation genes (nod and nol genes) is under both positive and negative controls. These genes are activated by the products of the three related nodD genes, in conjunction with signal molecules from the host plants. We showed that negative regulation is mediated by a repressor protein, binding to the overlapping nodD1 and nodA as well as to the nodD2 promoters. The encoding gene, termed nolR, was identified and cloned from strain 41. By subcloning, deletion and Tn5 mutagenesis, a region of 594 base-pairs was found to be necessary and sufficient for repressor production in strains of R. meliloti lacking the repressor or in Escherichia coli. Sequence analysis revealed that nolR encodes a 13,349 Da protein, which is in agreement with the molecular weight of the NolR protein, determined after purification by affinity chromatography, utilizing long synthetic DNA multimers of the 21 base-pair conserved repressor-binding sequence. Our data suggest that the native NolR binds to the operator site in dimeric form. The NolR contains a helix-turn-helix motif, which shows homology to the DNA-binding sequences of numerous prokaryotic regulatory proteins such as the repressor XylR or the activator NodD and other members of the LysR family. Comparison of the putative DNA-binding helix-turn-helix motifs of a large number of regulatory proteins pointed to a number of novel regularities in this sequence. Hybridizations with an internal nolR fragment showed that sequences homologous to the nolR gene are present in all R. meliloti isolates tested, even in those that do not produce the repressor. In another species, such as Rhizobium leguminosarum, where NodD is autoregulated, however, such sequences were not detected.

Amino Acid Sequence↗
PubMed1996

Analysis of tumor spillage during radical prostatectomy using RT-PCR of prostate specific antigen.

Recent reports have suggested the shedding of cancer cells during radical extirpation of tumors. Prostate cells can be expressed from the prostate ex vivo and found in the expressed prostatic secretions. We conducted an in vivo study to determine if prostate epithelial cells can be found in the operative site as determined by RT-PCR targeted at prostate specific antigen (PSA) and to correlate this with pathologic stage and outcome. We analyzed 14 consecutive radical retropubic prostatectomy procedures with a minimum 1-year follow-up. Intraoperatively, 5-10 ml of fluid (representing blood, urine, and irrigant) was aspirated from the operative field at three time points: after transaction of the dorsal vein complex, urethra, and bladder neck. Ficoll gradient fractionation was carried out on the specimens, and RNA was extracted from the cell pellet. Quality of RNA and presence of the PSA mRNA was determined by RT-PCR targeted at actin and PSA, respectively, using previously published primers. The medical records were reviewed for pathologic stage. There were nine patients with extraprostatic disease and five patients with organ confined disease. Five of 14 (36%) patients tested positive for prostate epithelial cells in the operative field at one or more points during radical prostatectomy. All five positive RT-PCR PSA assays were in patients with locally advanced disease, whereas all of the patients with organ-confined disease were negative for RT-PCR. This preliminary in vivo study suggests that locally advanced prostate cancer may be associated with PSA expressing cells in the operative field during radical prostatectomy. The clinical significance of this is unclear, but this finding suggests that shedding of cells in the operative field is more likely with locally advanced disease.

Combined Modality Therapy↗
PubMed1995

Transformational leadership and job satisfaction.

The relationship of head nurse leadership style to staff nurse job satisfaction was investigated using the leadership paradigm of transformational and transactional leadership. The Multifactor Leadership Questionnaire and the Index of Work Satisfaction were completed by 122 staff nurses employed throughout acute care community hospitals in many patient care areas. Correlations showed a significant positive relationship between those head nurses exhibiting a transformational leadership style and the job satisfaction of their staff nurses.

Factor Analysis, Statistical↗
PubMed2001

The impact of 1990 Medicaid drug rebates policy on access to prescriptions.

The U.S. Congress passed a new law (PL 101-508) in 1990 requiring the pharmaceutical manufacturers to grant rebates on prescription drugs sold at retail under the federal Medicaid program. The goals include containing Medicaid program costs and expanding access of the indigent to ethical drug treatments. This paper evaluates the impact of HCFA's mandatory federal upper limit (FUL) prices on the retail sales volume of prescription drugs during 1994. Data of the most frequently dispensed drugs, as measured by the National Prescription Audit of IMS America, are used. Regression model results suggest that the competing drug prices impact sales significantly, and Medicaid drug rebates expand access to drug interventions by stimulating retail transactions. Prescription sales are also own-price insensitive (inelastic) in Medicaid and non-Medicaid market segments. The implications of extending Medicaid prescription drug rebates policy to enrollees in the traditional Medicare program are examined.

Drug Industry↗
PubMed1994

Phosphorylation of the p34 subunit of human single-stranded-DNA-binding protein in cyclin A-activated G1 extracts is catalyzed by cdk-cyclin A complex and DNA-dependent protein kinase.

The human single-stranded-DNA-binding protein (HSSB, also called RP-A) is a trimeric complex (p70, p34, and p14) required for multiple functions in DNA transactions. We report here that the p34 subunit of HSSB was hyperphosphorylated by kinase activities present in G1 extract (obtained from HeLa cells in G1 phase) preincubated with human cyclin A. This hyperphosphorylated HSSB product included at least four species of p34 that migrated more slowly through denaturing polyacrylamide gels than the hypophosphorylated form. Fractionation of cyclin A-activated G1 extract identified two kinases involved in the hyperphosphorylation of HSSB p34: cdk-cyclin A complex and DNA-dependent p350 protein kinase (DNA-PK). Kinetic analysis revealed that in cyclin A-activated G1 extract, p34 was first phosphorylated by cdk-cyclin A prior to the action of DNA-PK. Addition of p21cip1, a specific inhibitor of cdk-cyclin A but not DNA-PK, nearly abolished the hyperphosphorylation of HSSB p34 in G1 extract preincubated with cyclin A. This suggests a requirement of the cdk-cyclin A activity for the phosphorylation of p34 by DNA-PK in G1 extract.

CDC2-CDC28 Kinases↗
PubMed1998

System response of the sinoatrial node during vagal stimulation.

The SA node response to modulations in canine vagal tone was investigated by means of the heart rate variability power spectrum. A new algorithm that was developed for accurate power spectrum estimation of short R-R segments is described. The performance of the algorithm was assessed for ECG recordings obtained from a controlled experiment, in which a frequency modulated pulse train was applied to the vagal nerve after vagal transaction and blockade of the sympathetic system. The power spectrum calculated for 20-25 heartbeats showed conspicuous spectral peaks in accordance with the different modulating frequencies between 0.1 and 0.3 Hz. The presence of spurious peaks was negligible even when the analysed signal segment consisted of only 20-25 beats. These results imply that for a certain range of modulating frequencies the sinoatrial node responds linearly to fluctuations in the parasympathetic tone. System identification methods that include fitting a linear model to the heart rate variability signal and analysis of the residuals were used for confirming the hypothesis of linearity. For higher frequencies of the modulating signal, usually above 0.3 Hz, the system was found to deviate from linearity.

Animals↗
PubMed2005

[Communication deviance and thought disorder in schizophrenia: a case study].

INTRODUCTION: Communication deviance (CD) means forms of communication that are hard to follow and that make difficult the consensual sharing of attention and meaning, and it has been hypothesized as a contributor to psychopathology of offspring, including schizophrenia. There is increasing evidence that CD may also trigger relapse of symptomatic illness in remission. We present a case with detailed sequence analysis, where we demonstrate disordered functioning in a family of a 27-year-old male patient with schizophrenia. METHOD: The patient and his parents participated in the Consensus Rorschach (CR) situation, a common problem solving task. Behaviour during the interaction was coded and analysed on different levels, including relational and symbolic level; and also, focusing on specific patterns of thought disorder. Results are in accordance with former findings that CD level is high in families of patients with schizophrenia. This type of communication was found to initiate transactional processes which resemble "games" aimed at gaining control over other family members. The content level of communication was frequently immature, and communication reflected pseudo-dependence as an only form of conflict resolution. CONCLUSIONS: Findings from the present study may be important from the perspective of secondary and tertiary prevention, and they reflect need for intervention focusing on the whole family.

Adult↗
PubMed2006

Facilitating interpersonal interaction and learning online: linking theory and practice.

INTRODUCTION: An earlier study of physicians' perceptions of interactive online learning showed that these were shaped both by program design and quality and the quality and quantity of interpersonal interaction. We explore instructor roles in enhancing online learning through interpersonal interaction and the learning theories that inform these. METHODS: This was a qualitative study using focus groups and interviews. Using purposive sampling, 50 physicians were recruited based on their experience with interactive online CME and face-to-face CME. Qualitative thematic and interpretive analysis was used. RESULTS: Two facilitation roles appeared key: creating a comfortable learning environment and enhancing the educational value of electronic discussions. Comfort developed gradually, and specific interventions like facilitating introductions and sharing experiences in a friendly, informative manner were helpful. As in facilitating effective small-group learning, instructors' thoughtful use of techniques that facilitated constructive interaction based on learner's needs and practice demands contributed to the educational value of interpersonal interactions. DISCUSSION: Facilitators require enhanced skills to engage learners in meaningful interaction and to overcome the transactional distance of online learning. The use of learning theories, including behavioral, cognitive, social, humanistic, and constructivist, can strengthen the educational design and facilitation of online programs. Preparation for online facilitation should include instruction in the roles and techniques required and the theories that inform them.

Attitude of Health Personnel↗
PubMed2006

The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation.

Ribosome biogenesis is a complex process that requires >150 transacting factors, many of which form macromolecular assemblies as big and complex as the ribosome itself. One of those complexes, the SSU processome, is required for pre-18S rRNA maturation. Although many of its components have been identified, the endonucleases that cleave the pre-18S rRNA have remained mysterious. Here we examine the role of four previously uncharacterized PINc domain proteins, which are predicted to function as nucleases, in yeast ribosome biogenesis. We also included Utp23, a protein homologous to the PINc domain protein Utp24, in our analysis. Our results demonstrate that Utp23 and Utp24 are essential nucleolar proteins and previously undescribed components of the SSU processome. In that sense, both Utp23 and Utp24 are required for the first three cleavage steps in 18S rRNA maturation. In addition, single-point mutations in the conserved putative active site of Utp24 but not Utp23 abrogate its function in ribosome biogenesis. Our results suggest that Utp24 might be the elusive endonuclease that cleaves the pre-rRNA at sites A(1) and/or A(2.).

Base Sequence↗