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At least 721 records · Page 40Linked to original sources
PubMed2001

[Genetic polymorphisms of 9 STR loci in Achang ethnic group in Yunnan Province].

Blood samples were collected from the unreleatled individuals in the Achang ethnic group in Yunnan Province. Genetic distribution for nine STR loci and amelogenin locus were determined in Achang ethnic group based on GeneScan. 96 samples were denatured of gel electrophoresis. The databanks in Achang ethnic group were generated by using GeneScan, genotyper, and genetic distribution analysis. 69 alleses and 166 genotypers were observed, with the corresponding frequency being 0.0050-0.6100 and 0.0100-0.3900. The average H is 0.7381. The comulated DP is 0.9999999. The comulated EPP is 0.9999989. The allele distribution of the loci was in good agreement with the Handy-Weibeng equilibrium. It is useful to establishing DNA databanks for studying gene natural resources, very valuable in the study of forensic science, anthropology and ethnic.

Amelogenin↗
PubMedDate not supplied

[Proteolytic preparation from the culture of Bacillus polymyxa str. 13-13].

A proteolytic preparation has been isolated from the culture fluid filtrate Bacillus polymyxa str. 13-13 by salting out with (NH4)2SO4 (0.7 of complete saturation). The data characterizing the amino acid composition and the enzymic system of the resultant preparation are given. It has been shown that the proteolytic activity depends on the enzyme containing metal ion, i. e. metal enzyme.

Amino Acids↗
PubMedDate not supplied

[Multiple forms of acid phosphatase of the mould Aspergillus awamori str. 22].

By isoelectric focusing and DEAE-cellulose and Sephadex G-150 chromatography it has been shown that extracellular acid phosphatase produced by Aspergillus awamori str. 22 exists as a mixture of oligomers of molecular weights ranging from 30,000 to 140,000. With an increase of aggregation of molecules of acid phosphatase the isoelectric point increases from 4.6 to 5.4.

Acid Phosphatase↗
PubMed2001

[Comparison study of the HUMFGA and HTPO STR system in the Saxon and Czech population].

High variability of repetitive DNA in a non-coding region of human genome affords possibilities for vestige identification and determination of their origin. For analysis of DNA many a time obtained from decomposed material, usually in a very small amount and sometimes partially degraded, polymerase chain reaction is considered as optimal technique. For identification of individuals is used an Amplified Fragment Length Polymorphisms (AmpFLP), which present repetitions 10 to 70 base pairs long in an amplification products of 170 to 1200 bp. In legal medicine PCR systems using tetrameric repetition polymorphism are widely used. These systems are highly sensitive, but in comparison to AmpFLP systems, imperceptibly influenced by nucleic acid degradation in cases of old or partially degraded samples. Aim of our study was to compare frequency data for 2 STR-systems, FGA and HTPO, both after PCR amplification, on 200 samples of Czech and 228 of German population. In FGA system of Czech population 14 different alleles and 44 genotypes were determined, while German population provided 13 different alleles and 47 genotypes. Furthermore, frequency of alleles and heterozygosity rate were determined. Heterozygosity rate for Czech samples was 85%, for German ones 87.1%. Alleles 21 and 22 were most frequent (approx. 20%) between both Czech and German population, followed by alleles 20, 23 and 24 (14-12%). Besides these data, three different transient alleles were detected. In HTPO systems of both population groups 12 different genotypes, consisting of 5 alleles, were determined. Genotypes 8/8 and 8/11 observed in 30% were the most frequent ones in both population groups. Heterozygosity rate for Czech samples was 62.5%, for German ones 65%. Results were statistically analyzed. In both population groups, no significant difference from Hardy-Weinberg's balance was found. Additionally, statistic parameters for forensic effectivity evaluation were constructed. Comparison of both populations using homogeneity tests provided non-significant difference. FGA system provides extraordinary high forensic exploitativeness and therefore is fully acceptable for vestige identification and determining of their origin. For significant determination of transient alleles analysis of fluorescein-labelled fragments in denaturing gel system should follow, using automated laser detection of fluorescence. Polymorphism of HTPO-system is significantly lower compared to FGA-system, but relatively small PCR product required as well as simple detection and imperceptible influence of nucleic acid degradation allow the use of HTPO for analysis of highly degraded DNA.

Czech Republic↗
PubMed2002

Skeletal remains presumed submerged in water for three years identified using PCR-STR analysis.

We describe the successful identification of the remains of a saponified body found in a dam by typing of nuclear DNA. Whereas DNA extracted from soft tissues yielded negative PCR results, DNA extracted from the bone by a slightly modified Qiagen procedure allowed the typing of sex (AMG locus) and of 10 additional STR loci. An identity document was found belonging to a man missing for 3 years and comparison of the results to the DNA profiles of his son and wife confirmed the identity. The longest delay reported until now for successful nuclear DNA genotyping after immersion in river water was 18 months. This case demonstrates a delay of up to 3 years.

Bone and Bones↗
PubMed2001

[A study of genetic polymorphism of the STR locus D2S441 in Chinese Han population in Chengdu].

Using PCR and PAG, followed by silver staining, the tetrameric STR D2S441 locus was studied in 260 unrelated Chinese individuals living in Chengdu. 9 alleles and 26 genotypes were observed. The range of fragment size was 131 bp to 155 bp. The genotype distribution of D2S441 locus in Han population was in accordance with Hardy-Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The discriminating power (Dp), observed heterozygosity (H), polymorphism information content (PIC) and power of exclusion (PE) were 0.9084, 0.7885, 0.7390 and 0.5778 respectively. The results demonstrated that this locus was highly polymorphic and could be used for forensic identification and paternity testing.

Alleles↗
PubMed1999

[Study of the high polymorphism STR locus-FGA in Chinese population].

Population genetic study of the complicated STR locus-FGA was performed by the method of PCR in Han population samples. A total of 21 different alleles including 7 interalleles and 63 different genotypes were observed in 349 unrelated individuals. The observed genotype discrimination showed no significant deviation from Hardy-Weinberg equilibrium and 51 family studies showed no mutation. According to the results obtained in this study(DP = 0.9612, PE = 0.7007, PIC = 0.8333), this system can be used as an useful means in forensic identification of both criminal and paternity case.

Asian People↗
PubMed2000

[Polymorphism of 13 STR loci for establishment of Chinese criminal DNA database].

Allele frequency distributions for thirteen STR loci: D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, THO1, TPOX, CSF1PO, and D7S820, have been determined by multiplex amplification and subsequent automatic fluorescent detection. The result of statistical analysis shows that the 13 loci satisfy Hardy-Weinberg expectation. The data obtained from this study will be used as reference data for forensic DNA analysis in China.

Alleles↗
PubMed2002

[Genetic polymorphism of six STR loci in the Han population in Henan province].

To investigate the allele frequencies of six short tandem repeats (STR) loci: F13A1, F13B, D8S1179, CSF1PO, D5S818 and TPOX in Han population in Henan province, DNA was extracted with chelex from EDTA-blood samples of the unrelated individuals in Henan province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silverstain. The results were obtained through genetical analysis. The authors got the number of allele of six loci, Three alleles of F13A1 could be detected in this population. The F13A1 * 7 is the most common allele with a frequency of 45.2%, followed by F13A1 * 5 and F13A1 * 4 respectively. F13B had four alleles among which F13B * 10 has the highest frequency, followed by F13B * 9, F13B * 8, F13B * 11. The 8 alleles of D8S1179 in the order from high to low frequency is D8S1179 * 14, * 15, * 12, * 11, * 10. And the most common frequencies of alleles of CSF1PO, D5S818, TPOX ore CSF1PO * 12, D5S818 * 11, TPOX * 8, respectively followed by the allele of * 11, * 14, * 10, * 13 in CSF1PO locus, and * 12, * 13, * 10, * 9, * 14, * 8, * 15, * 7 in D5S818 locus, * 11, * 9, * 12, * 10 in TPOX locus. The heterozygosities of the six loci were 0.62, 0.46, 0.83, 0.59, 0.78 and 0.65; the discrimination powers were 0.78, 0.66, 0.95, 0.79, 0.92 and 0.82. The heterozygosities of the six loci are high and the frequencies are in good agreement with Hardy-Weinberg equilibrium. The six loci are good as genetic markers. We can use these loci in dividualidentification and partenity test in forensic area.

Alleles↗
PubMed2003

[Genetic polymorphism of 3 STR loci of CSF1PO, TPOX and TH01 in Chinese Sibo population].

OBJECTIVE: To obtain allele and genotype frequencies and related forensic data of CF1PO, TPOX and TH01 loci in Chinese Xinjiang Sibo population. METHODS: Genomic DNA from peripheral blood mononuclear cells of normal Chinese Xinjing Sibo population was used as template, and CSF1PO, TPOX and TH01 fragments were amplified by PCR. The PCR products were analyzed by 4% denaturing PAGE and detected using silver stain detection. RESULTS: Nine alleles were found at CSF1PO locus, eight alleles at TPOX locus and eight alleles at TH01 locus in Chinese Sibo population. All the 3 loci complied with Hardy-Weinberg equilibrium. The heterozygosities were 0.9426, 0.8361 and 0.8853, and the polymorphism information contents were 0.8298, 0.7213 and 0.7626 for CSF1PO, TPOX and TH01, respectively. CONCLUSION: The data on the alleles frequency of these 3 STR loci might be used for individual identification and paternity identification and for genetic researches in Chinese Sibo population.

Alleles↗
PubMed2002

[Genetic polymorphism of the STR DXS9898 locus in Han population].

OBJECTIVE: Studying the genetic polymorphism of X-STR locus DXS9898 in Han population. METHODS: 296 unrelated Chinese individuals (199 females and 97 males) living in Chengdu were investigated using PCR and PAG electrophoresis followed by silver staining. RESULTS: 6 alleles were observed and the range of fragment size was 189-214 bp. The genotype distribution of DXS9898 locus was in accordance with Hardy-Weinberg equilibrium. Family survey confirmed Mendelian inheritance of alleles. The observed heterozygosity in females was 0.5930, the discriminating power (Dp) were 0.5667 and 0.9420 for males and females respectively. The power of exclusion were 0.5862 and 0.4392 for trio and duo respectively. CONCLUSION: The results demonstrated that the locus is highly polymorphic and can be used in forensic identification and parentage testing.

Alleles↗
PubMed2003

[Polymorphism of PentaD and PentaE STR locus in five Chinese Han population].

OBJECTIVE: To obtain the genetic polymorphism data of Guangxi, Hunan, Henan, Sichuan, Taiwang Chinese Han population and compare the polymorphism of PentaD and PentaE STR locus. METHODS: The two loci was analyzed by using the PowerPlex 16 System. RESULTS: 10 alleles of PentaD and 19 alleles of PentaE were found in the five Han population. PentaD and PentaE have the expected heterozygosity values of 0.7746-0.8047 and 0.9005-0.9219, the polymorphism information content values of 0.7710-0.8025 and 0.8969-0.9176, the discrimination power values of 0.9223-0.9341 and 0.9471-0.9782, the power of exclusion values of 0.5435-0.6325 and 0.6785-0.8465, respectively. CONCLUSION: The result showed that these two loci were highly informative and suitable for forensic application.

Alleles↗
PubMed2003

STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes.

Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplification under 15 different template DNA to PCR reaction volume ratios. Reduction of PCR reaction volume and DNA down to 10 microL and 0.500 ng, respectively, produced identical profiles with the same signal intensity and heterozygous allele peak height ratio (HR). Reduction to 5 microL and 0.063 ng yielded HR values that were slightly affected in one to three STR loci. PCR reaction volume reduction can enhance detection and sensitivity while reducing the consumption of irreplaceable crime scene samples.

Case-Control Studies↗
PubMedDate not supplied

[Polymorphism of the STR system LPL in the Silesian population].

This paper shows the results of locus LPL in the population of Upper Silesia. The examinations were performed in a group of 147 unrelated adults. The following gene frequencies were observed: LPL*9 = 0.0578; LPL* 10 = 0.4048; LPL* 11 = 0.2517, LPL* 12 = 0.2449 i LPL* 13 = 0.0408. DNA was isolated using Blood DNA Prep Plus of A&A Biotechnology. Amplification was carried out in a Perkin Elmer GeneAmp PCR System 2400 thermal cycler, using the GenePrint STR System LPL (8p22) of Promega Corporation, Madison, WI, USA. PCR products were electrophoretically separated by high-resolution polyacrylamide gel--GDG of Perkin Elmer. Gels were stained by the silver method. Test chi 2, exact and Carmody's were used for statistical estimation. The identification values of the system are: PD, Ht, PM, MEC, PIC, MEP. The analysis of studies has shown that the examined population is in the genetic equilibrium conformable to the Hardy-Weinberg's principle. The estimated gene frequencies are similar to those observed in two Polish populations.

Adult↗
PubMedDate not supplied

[Population genetics of the 10 short tandem repeat (STR) loci in the population of Podlasie (Northeastern Poland)].

A population study on 10 STR loci: D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01 and FGA was performed on 620 unrelated individuals born in Podlasie (NE Poland). The DNA was amplified by multiplex PCR using a commercially available multiplex AmpFISTR SGM Plus kit. Capillary electrophoresis with fluorescent detection was carried out using the ABI 310 Genetic Analyzer. The expected performance of the analysed loci for personal identification and paternity testing was estimated. All loci met the Hardy-Weinberg assumption. No significant differences between the studied population and other Polish data were found. The combined values of the Matching Probability and of the Power of Exclusion are 4.18 x 10(-13) and 0.99997, respectively.

Adult↗
PubMedDate not supplied

[Genetic variation at STR-TH01 locus in the South Polish population].

We report on a rare allelic variant of the TH01 STR locus. Within this commonly used tetranucleotide repeat system, we identified and characterized by denaturating gel electrophoresis an allele of the size corresponding to 10.3 repeats, analogous to the most common 9.3 allele. The novel allele segregated from a putative father to the child in a paternity case. We discuss the advantage of high resolution second generation multiplex systems (SGM) using internal size standards during electrophoresis.

Alleles↗
PubMed2004

STR polymorphisms in Philippine ethnolinguistic groups: evaluation of forensic utility.

Population data was collected for the STR loci F13AO1, FES/FPS, HUMvWA, and HUMTHO1, in three major Philippine ethnolinguistic groups and used to estimate statistical parameters for identity testing in forensic work on Filipinos. The Cebuano, Ilocano, and Pampango populations in the Philippines were studied because they are among the biggest linguistic groups in the country, thus their genotypic profiles should substantially represent those of many Filipinos. The number of alleles varied from 4 to 9 at all loci, falling within the range observed for other local and world populations. Pairwise comparisons of the allele frequency distributions showed no statistical differences among the populations. The test for linkage equilibrium showed no evidence of non-random association of alleles across the physically unlinked loci in any of the three populations. The four loci combined gave an exclusion power of > or =0.9995 and a power of paternity exclusion of 0.8859-0.9389.

Alleles↗