Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Lipid modification”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 721 records · Page 40Linked to original sources
PubMedDate not supplied

[Modification of the lipid composition of neuroblastoma C1300 cells using liposomes].

An essential increase in the total cholesterol content with a significant growth in the level of its esters was observed in neuroblastoma C1300 cells differentiated by means of 5'-bromodesoxyuridine and incubated for 1 h with lecithin-cholesterol liposomes (1:1 mol/mol). These cells also displayed an increased amount of polyunsaturated fatty acids in the composition of lecithin molecules and the appearance of lysolicethin as well. Incubation of the cells with lecithin liposomes was accompanied by a decrease in the content of cholincholesterol in the cells. In this case the level of cholesterol esters decreased to a greater extent than the level of free cholesterol. In lecithin molecules of these cells there occurred a relative increase of saturated fatty acids. The cells can retain viability and compensate changes caused by cholesterol excess during their incubation with lecithin-cholesterol liposome for 60-90 min. A longer incubation with liposomes results in a sharp drop of cell viability.

Cell Line↗
PubMed1985

[Modification of serum lipids, lipoproteins and apoproteins AI and B in patients with hyperlipidemia Type IIa and IIb using polyenylphosphatidylcholine].

In a double blind study 27 patients with type II hyperlipidemia (8 IIa and 19 IIb) were treated as follows: 13 received placebo and 14 sn-polyenylphosphatidylcholin (PPC) (P 0206/1/01, Nattermann GmbH, Cologne) in a dose of three times 450 mg b.i.d. In all patients, and also in the two subclasses of patients with type IIa and type IIb hyperlipidemia, total cholesterol and LDL cholesterol were lowered significantly by PPC. The other parameters showed only minor variation. There was a downward trend in apoprotein B, triglycerides and VLDL cholesterol, and an upward trend in apoprotein AI, with virtually unchanged HDL cholesterol. None of these variations was significant compared with placebo. The fall in LDL cholesterol with unchanged HDL cholesterol caused a statistically significant decrease in the LDL cholesterol/HDL cholesterol ratio, thus supporting the hypothesis of an antiatherogenic property of PPC, as demonstrated experimentally in various animals.

Adult↗
PubMed1975

Immunochemical studies of lipids. IV. Chemical modification of Forssman globoside and immunological activities.

N-deacylated and N-deacetylated Forssman globoside obtained from Forssman globoside by partial alkaline hydrolysis had no Forssman activity. After re-N-acetylation of the N-deacylated and N-deacetylated Forssman globoside, the N-acetylsphingosyl Forssman oligosaccharide thus obtained recoverd its activity. Moreover, after ozonolysis and reduction of the N-acetylsphingosyl Forssman oligosaccharide, the 2-N-acetoamido-1,3,4-trihydroxybutanoyl Forssman oligosaccharide thus obtained still had a haptenic activity, but couldn't show a precipitin reaction with Forssman antibody. On the other hand, the Forssman globoside and the N-acetylsphingosyl Forssman oligosaccharide gave rise to precipitin line on agar gel double diffusion, it was thus concluded that the aqueous solution of these substances formed spherical micelles to behave like a fairly macromolecular multivalent antigen.

Acetylation↗
PubMedDate not supplied

[Modification of the lipid component of bacterial membranes by the introduction of lipotropic compounds into lyophilized cells].

In order to elucidate potentialities of modifying the lipid component of bacterial membranes, lyophilized cells of M. lysodeikticus, E. coli and other bacteria were treated by hydrophobic compounds dissolved in the organic solvent, with the latter subsequently removed by evaporation or freezedrying prior to cell rehydration. The data obtained by means of spin-probes, fluorescent spectroscopy, electron microscopy, photoreactive label and other methods suggest that following bacteria rehydration at least part of the substance occurs in the membrane structures. The amount of the substance involved in bacterial cells depends on the type of compound and on whether bacterial cells belong to gram-positive or gram-negative microorganisms: the substance content of the latter is greater than of the former.

Escherichia coli↗
PubMed1984

Modification of microsomal lipid peroxidation and drug metabolism by cytoplasmic copper.

NADPH-dependent microsomal lipid peroxidation (LPO) can be influenced by addition of cytoplasmic supernatant as was demonstrated by Gram and Fouts (Arch. Biochem. Biophys. 114, 331-335, 1966). Small quantities of hepatocellular supernatant stimulate LPO, higher concentrations are inhibitory. These opposed effects depend on the relative concentrations of copper and iron, either as ions or protein-bound. A mathematical model is given to predict the formation of malondialdehyde as indicator of LPO in dependence of these metals. The copper-containing superoxide dismutase was shown to be without effect on LPO, whereas coeruloplasmin was inhibitory. Inhibition of LPO by copper ions or by TRIS buffer increased the linearity of microsomal ethylmorphine demethylase.

Animals↗
PubMed1984

[Effect of modification of the lipid bilayer on the functioning of the electron transport chain of endoplasmic reticulum membranes in nitrosodiethylamine-induced hepatic carcinogenesis].

The kinetics of cytochrome P-450 content in endoplasmic reticulum membranes at different stages of hepatocarcinogenesis was studied under the influence of unsaturated fatty acids by the EPR method. At early stages of carcinogenesis the preparations containing oleic, linoleic and arachidonic acids prevent changes in the cytochrome P-450 content usually observed in case of nitrosodiethylamine introduction. The diet containing unsaturated fatty acids has a stabilizing effect revealed by the similar changes in the content of cytochrome P-450, phosphatidyl choline and fatty acids composition and also in the level of organization of the endoplasmic reticulum membrane lipid bilayer.

Animals↗