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PubMed2005

Constituents of the leaves and twigs of Calyptranthes pallens collected from an experimental plot in Southern Florida.

Fractionation of the chloroform-soluble extract of the leaves and twigs of Calyptranthes pallens, collected from an experimental plot in a hardwood forest of southern Florida, using a hormone-dependent human prostate carcinoma (LNCaP) tumor cell line, led to the isolation of a phloroglucinol derivative with a novel carbon skeleton, pallenic acid (1), and a new triterpenoid, 3beta-hydroxy-18alpha,19alpha-urs-20-en-28-oic acid (2). The known compound methylene-bis-aspidinol (3) was selectively active against the human oral epidermoid carcinoma (KB) cell line. Several known compounds of the ellagic acid, lignan, phloroglucinol, sterol, and triterpene types were also obtained in the present investigation.

Antineoplastic Agents, Phytogenic↗
PubMed2002

Diel periodicity of emergence of adult citrus rust mites in central Florida.

Emergence of adult citrus rust mites, Phyllocoptruta oleivora (Ashmead) from the imagochrysalis occurred with a diel periodicity that peaked between mid morning and early afternoon. The pattern of emergence did not differ for mites reared from eggs held under different photoperiod regimes. The transition of nymphs to the imagochrysalis stage also showed a diel periodicity, occurring in the evening. The average duration of the imagochrysalis stage at 28 degrees C was 14-15 h. Wetting by rain or dew was simulated by immersing fruit in water each night, but the transition to the imagochrysalis stage or the subsequent emergence pattern of adults was not affected. Adult mites reared from eggs laid during two, consecutive 12-h periods emerged at the same time of day, but the peaks of emergence were separated by 24 h. Eclosion of larvae from eggs laid during two, consecutive 12-h periods did not show a diel periodicity. Eggs continuously immersed in water during their development did not hatch, whereas there was a small, but significant, reduction in the viability of eggs immersed in water each night. These results are discussed in relation to external cues that may entrain the diel patterns of development and adult emergence, and to the adaptive responses of citrus rust mite to cyclic fluctuations of key environmental variables in central Florida.

Animals↗
PubMed1997

The impact of Hurricane Andrew on deviant behavior among a multi-racial/ethnic sample of adolescents in Dade County, Florida: a longitudinal analysis.

Findings from a longitudinal study are presented on the relationships between the problems and stresses resulting from Hurricane Andrew and posthurricane minor deviant behavior. The sample (N = 4,978) included Hispanic, African-American, and White non-Hispanic middle school students enrolled in Dade County, Florida public schools. Two waves of data were collected prior to the hurricane; a third was obtained approximately 6 months following the storm. Results indicated that females were likely to report higher levels of hurricane-related stress symptoms than males. After controlling for prehurricane levels of minor deviance, family support, and race/ethnicity, hurricane stress symptom level remained a significant predictor of posthurricane minor deviant behavior. The findings lend support to stress theories of social deviance.

Adolescent↗
PubMed2004

Predictors of HIV risk among Hispanic farm workers in South Florida: women are at higher risk than men.

This study examined factors associated with being at risk of sexually acquiring HIV among a community sample of 244 Hispanic migrant and seasonal farm workers. Bilingual staff interviewed respondents anonymously at worksites, camps, and other public venues in South Florida during the 2002 winter/spring growing season. The following variables were positively associated with being at risk of sexually acquiring HIV in multivariable analyses: being female; being married; having "some" or "a lot" of knowledge about HIV transmission, having ever used marijuana, having two or more sex partners in the last 12 months, and having had a sexually transmitted infection. The findings heighten the importance of recognizing women's elevated risk of HIV infection and conducting further studies to examine the factors associated with this increased risk. The study is an important first step toward developing tailored HIV prevention interventions for this at-risk, understudied population.

Adolescent↗
PubMed2004

Elements of South Florida's Comprehensive Everglades Restoration Plan.

Approximately 70% less water flows through the Everglades ecosystem today compared with the historic Everglades, and the quality of the remaining water is often degraded. The regionally managed hydropattern does not follow the pre-drainage distribution, timing, and duration of the natural Everglades, nor can water move freely though the remaining Everglades. As a result, there have been significant reductions in wildlife and fish populations, their habitat, and the environmental services wetlands provide society. Both the problems of declining ecosystem health and the solutions to Everglades restoration center on restoring the quantity, quality, timing, and distribution of water. The Comprehensive Everglades Restoration Plan consists of over 60 civil works projects that will be designed and implemented over a 30 year period. At an estimated cost of 7.8 billion dollars, it seeks to correct an earlier attempt at water management in South Florida and improve water availability during the dry season and reduce flooding of urban and agricultural areas during the wet season. The plan calls for storage and controlled release from more than 217,000 acres of new reservoirs and wetland-based treatment areas and from over 300 underground aquifer storage and recovery wells. The plans assumes that during retention in stormwater treatment areas, the excess phosphorus, nitrogen, agrichemicals such as atrazine, diazinon, endosulfan, and other contaminants will be reduced before release into the natural areas. It also assumes that little or no change in water quality will occur during underground storage. To improve the hydraulic connectivity of natural areas, some of the extensive system of levees and canals within the Everglades will be removed in an effort to improve overland water flow. Most of the current planning has focused on water storage and restoring basic hydrology in the remnant natural areas and on phosphorus removal as a benchmark of water quality. The restoration plan, as approved by Congress, is conceptual and most of the details, including potential impacts of the plan on the natural system and the role of contaminants remain to be evaluated.

Animals↗
PubMed2004

Cultural feasibility assessment of tuberculosis prevention among persons of Haitian origin in South Florida.

A cultural feasibility study was conducted among persons of Haitian origin in South Florida to identify factors which might influence utilization of screening and treatment services for latent tuberculosis infection in this population. Five focus group interviews conducted among men and women explored cultural beliefs and practices related to TB, barriers and incentives to screening, and approaches to increasing treatment adherence. Key findings include the influence of social stigma and fears related to confidentiality of medical status as disincentives to screening. Cultural sensitivity to being labeled as a high risk group for these infections also emerged as a critical variable. Community-based approaches to health education for this population are described. Study recommendations include the planning of programs based on a service delivery model that stresses respect and personal attention to clients, improved interpersonal skills of health center staff, and coordination of services between private doctors and public health agencies.

Attitude to Health↗
PubMed2004

Reliability of sexually violent predator civil commitment criteria in Florida.

The present study investigated the reliability of Sexually Violent Predator (SVP) civil commitment criteria under Florida's Jimmy Ryce Act. The purpose of the study was to determine if, independently, 2 evaluators would reach the same conclusions about the same client (n = 295). According to civil commitment criteria outlined by the United States Supreme Court (Kansas v. Hendricks, 1997), SVPs must display a mental abnormality predisposing them to sexual violence and a likelihood of future sexual violence. The interrater reliability of 8 DSM-IV diagnoses applied by evaluators to determine whether a client has a "mental abnormality that predisposes him to sexual violence" was found to be poor to fair (kappa = .23 to . 70). The interrater reliability of risk assessment instruments used to determine "likelihood of reoffense" was good (ICC = .77 to .85). The recommendations made by evaluators regarding whether or not to refer a client for civil commitment demonstrated poor reliability (kappa = .54). Implications for practice and policy are explored.

Adult↗
PubMed1999

Option-4 algorithm for Florida pocket depth probe: reduction in the variance of site-specific probeable crevice depth measurements.

Clinical periodontal measurement is plagued by many sources of error which result in aberrant values (outliers). This study sets out to compare probeable crevice depth measurements (PCD) selected by the option-4 algorithm against those recorded with a conventional double-pass method and to quantify any reduction in site-specific PCD variances. A single clinician recorded full-mouth PCD at 1 visit in 32 subjects (mean age 45.5 years) with moderately advanced chronic adult periodontitis. PCD was recorded over 2 passes at 6 sites per tooth with the Florida Pocket Depth Probes, a 3rd generation probe. The option-4 algorithm compared the 1st pass site-specific PCD value (PCD1) to the 2nd pass site-specific PCD value (PCD2) and, if the difference between these values was >1.00 mm, allowed the recording of a maximum of 2 further measurements (3rd and 4th pass measurements PCD3 and PCD4): 4 site-specific measure-meets were considered to be the maximum subject and tissue tolerance. The algorithm selected the 1st 2 measurements whose difference was < or = 1.00 mm (SPCD1 and SPCD2). If no 2 measurements had a difference < or = 1.00 mm, the examiner was required to select the 2 measurements closest to the rules of the algorithm. 4600 sites were available for analysis. 3992 sites (86.8%) required 2 recordings, 564 sites (12.3%) required 3 recordings and 44 sites (1%) required 4 recordings. Correlation coefficients for PCD1 and PCD2 and SPCD1 and SPCD2 were 0.83 and 0.96, respectively (p=0.00). Site-specific variances were calculated for PCD1 and PCD2 and SPCD1 and SPCD2. The mean of the PCD1/PCD2 site-specific variances (A) was 0.41 mm2 (range 0.00 mm2 to 33.62 mm2), whilst the mean of the SPCD1/SPCD2 variances (B) was 0.1 mm2 (range 0.00 mm2 to 2.0 mm2): the respective medians were 0.08 mm2 and 0.02 mm2. The study demonstrated high intra-examiner PCD agreement. The option-4 algorithm produced a reduction of 75.6% in the mean site-specific variance of PCD1/PCD2 (Y) (Y=[(A-B)/A]X 100) and a 75% reduction in the median site-specific variance of PCD1/PCD2.

Adult↗
PubMed1993

Thiopurine methyltransferase activity in a sample population of black subjects in Florida.

Thiopurine methyltransferase (TPMT), is an enzyme detected in the human red blood cell that catalyzes the S-methylation of thiopurine drugs and is known to exist as a genetic polymorphism in white subjects. Investigations in this laboratory of red blood cell TPMT showed interethnic differences also existed in North American black subjects. A sample group of black subjects in Florida had a mean activity of 8.64 +/- 3.47 U/ml red blood cells and an antimode of 6.5 units, which represented values significantly lower than those obtained for both the mean activity and the antimode in other populations. The findings of this study suggest the possibility that TPMT activity may be under genetic control in North American black subjects and that this ethnic group may be at greater risk of experiencing thiopurine-induced toxicity caused by the lower overall mean activity of the enzyme.

Adolescent↗
PubMedDate not supplied

Murder and cover-up could explain the Florida dental AIDS mystery.

This article reconsiders the murder theory regarding the transmission of the human immunodeficiency virus (HIV) from Dr David J. Acer to at least six dental patients which was initially dismissed by American investigators from the Centers for Disease Control and Prevention (CDC) and Florida Health and Rehabilitative Service Department (HRS). In the light of withheld behavioural evidence from medical records, legal testimonies, and personal interviews obtained during the investigation, the information presented here strongly supports the conclusion that these transmissions were most likely intended by the dentist to execute a political and social vendetta.

Acquired Immunodeficiency Syndrome↗
PubMed2004

Doctor of audiology (AuD) program at the University of South Florida.

This article describes the doctor of audiology (AuD) program at the University of South Florida (USF). The USF AuD program has been operational since the fall of 1999 and, to date, 47 students have graduated from either the postbaccalaureate track or the post-master's track. The faculty consists of 9 full-time and 8 part-time adjunct members who provide a curriculum that covers the audiology scope of practice. Being located in a major metropolitan area allows for diverse clinical practicum experiences that result in students being prepared to become autonomous professional practitioners.

Audiology↗
PubMed1999

Failure of Hydrotaea aenescens, a larval predator of the housefly, Musca domestica, to establish in wet poultry manure on a commercial farm in Florida, USA.

Hydrotaea aenescens (Weidemann) (Diptera: Muscidae) larvae and pupae were released in high-rise pullet houses in Pascoe County, Florida, U.S.A., for biological control of houseflies, Musca domestica L. (Diptera: Muscidae), in three separate tests during a 14-month period (April 1994-May 1995) and did not become established. However, H. aenescens believed to be from our releases did become established at a caged-layer farm 0.3 km from the release farm. Moisture in pullet house manure generally exceeded 80%, which is above the level at which H. aenescens is reared in colony. Caged-layer manure was also very wet, but crusted areas provided sites for H. aenescens larval development. Results demonstrate that H. aenescens adults will disperse from release sites and populate preferred sites without becoming a nuisance.

Animals↗
PubMed2002

Multiple paternity in loggerhead turtle (Caretta caretta) nests on Melbourne Beach, Florida: a microsatellite analysis.

Many aspects of sea turtle biology are difficult to measure in these enigmatic migratory species, and this lack of knowledge continues to hamper conservation efforts. The first study of paternity in a sea turtle species used allozyme analysis to suggest multiple paternity in loggerhead turtle (Caretta caretta) clutches in Australia. Subsequent studies indicated that the frequency of multiple paternity varies from species to species and perhaps location to location. This study examined fine-scale population structure and paternal contribution to loggerhead clutches on Melbourne Beach, FL, USA using microsatellite markers. Mothers and offspring from 70 nests collected at two locations were analysed using two to four polymorphic microsatellite loci. Fine-scale population differentiation was not evident between the sampled locations, separated by 8 km. Multiple paternity was common in loggerhead nests on Melbourne Beach; 22 of 70 clutches had more than one father, and six had more than two fathers. This is the first time that more than two fathers have been detected for offspring in individual sea turtle nests. Paternal genotypes could not be assigned with confidence in clutches with more than two fathers, leaving the question of male philopatry unanswered. Given the high incidence of multiple paternity, we conclude that males are not a limiting resource for this central Florida nesting aggregate.

Animals↗
PubMed2003

Extinction-colonization dynamics structure genetic variation of spotted sunfish (Lepomis punctatus) in the Florida Everglades.

The population genetics of aquatic animals in the Florida Everglades may be strongly influenced by extinction and colonization dynamics. We combined analyses of allozyme and microsatellite loci to test the hypothesis that two levels of population structure are present for spotted sunfish (Pisces: Centrarchidae: Lepomis punctatus) inhabiting the Everglades. We hypothesized that annual cycles of marsh dry-down increase local-scale genetic variation through a process of local extinction and colonization; we hypothesized that barriers to gene flow by levee/canal systems create a second, regional level of genetic variation. In 1996 and 1997, we sampled spotted sunfish from 11 Everglades sites that were distributed in three regions separated by levees. We documented patterns of genetic variation at 7 polymorphic allozyme loci and 5 polymorphic microsatellite loci. Most genetic variation was present among local populations, according to both types of genetic markers. Furthermore, samples from marsh sites were heterogeneous, while those from canals were not. These data supported our hypothesis that dry-down events and local population dynamics in the marsh have a significant effect on population genetic structure of spotted sunfish. We found no support for our hypothesis that water-management structures superimpose a second level of genetic structure on this species, possibly because canals obscure historical structure by facilitating gene flow or because the complete canal system has been in place for fewer than 20 generations of this species. Our data suggests a continent-island (canal-marsh) structure of populations with high gene flow among regions and recurrent mixing in marshes from canal and creek habitats.

Animals↗
PubMed1997

Cutaneous disseminated histoplasmosis in AIDS patients in south Florida.

BACKGROUND: Histoplasma capsulatum is a dimorphic pathogenic fungus endemic to the Mississippi and Ohio river valleys. In the immunocompetent it causes a self-limited disease, but in the immunocompromised may lead to disseminated disease (disseminated histoplasmosis (DH)). It is one of the opportunistic infections which defines the acquired immunodeficiency syndrome (AIDS) and is rarely encountered outside endemic regions. METHODS: Clinical, laboratory, and histologic information concerning seven patients with DH and AIDS in South Florida was recorded. RESULTS: We report seven cases of DH with mucocutaneous lesions in patients infected with the human immunodeficiency virus (HIV). All patients had markedly depressed CD4 counts of less than 40 cells/mm3, and only two had traveled to endemic areas. Two out of the seven patients were diagnosed with HIV/AIDS at the time DH was identified. All of our patients had mucocutaneous lesions at the time of diagnosis, which clinically presented as a generalized papular eruption, ulcers, and erythematous scaly plaques. CONCLUSIONS: Even in non-endemic regions, HIV-positive patients presenting with fever, chills, weight loss, hepatosplenomegaly, anemia, cough, lymphadenopathy, and mucocutaneous lesions should have an early skin biopsy specimen taken for mycologic tissue culture and histopathologic evaluation for disseminated fungal infections.

AIDS-Related Opportunistic Infections↗
PubMed2002

Office surgical incidents: 19 months of Florida data.

BACKGROUND: In 1999-2000 a series of sensational articles were published in the lay media emphasizing the hazards of office surgery. Since then 31 state medical boards or legislatures have, or are in the process of drafting regulations restricting office procedures. OBJECTIVE: To determine the nature, incidence and scope of injuries and deaths resulting from office procedures. METHODS: Mandatory reporting by physicians to a neutral central agency of all office surgical incidents that resulted in death, serious injury, or transfer to a hospital in the State of Florida from February 2000 to September 2001. Telephone and Internet follow up to determine reporting physician board status, hospital privilege status, and office accreditation status. RESULTS: In 19 months there were 43 procedure related-complications and eight deaths. Liposuction under general anesthesia was the single most common cause of incidents and deaths. There were no injuries or deaths reported with liposuction with tumescent anesthesia. 50% of offices reporting incidents or deaths were accredited by an independent accrediting agency. There were no incidents or deaths reported due to the anesthesia when using conscious sedation anesthesia, or intramuscular sedation or analgesia 98% of physicians reporting incidents or deaths had hospital privileges and were board certified. Anesthesiologists or nurse anesthetists provided all general anesthesia, and deep sedation. There were no physicians performing procedures outside their scope of specialty training. CONCLUSION: Liposuction under general anesthesia deserves closer scrutiny. Office accreditation is not associated with fewer patient injuries and deaths. Restrictions on tumescent liposuction, conscious sedation and intramuscular sedation and analgesia would not yield any saved lives or fewer injuries since these modes of anesthesia resulted in no injuries or deaths. Board certification and hospital privilege requirements for office practice would have very little effect since the vast majority of reporting physicians already had these credentials. These data do not show an emergent hazard to patients from office surgery This data strongly contradicts the lay media portrayal of the dangers of office procedures. Mandatory reporting of office incidents should be strongly supported, and this data should be available for analysis after protecting patient confidentiality.

Adverse Drug Reaction Reporting Systems↗
PubMed2005

Measuring patient acceptance of implantable cardiac device therapy: initial psychometric investigation of the Florida Patient Acceptance Survey.

INTRODUCTION: Patient acceptance of implantable device therapy has been established as an important outcome but the operationalization and validation of a measure of patient acceptance of implantable device therapy has not been fully completed. This study sought to validate a new measure of patient acceptance of cardiac implantable devices called the Florida Patient Acceptance Survey (FPAS). METHODS: The sample consisted of implantable cardioverter defibrillator (ICD; n = 58), and implantable atrioverter defibrillator (ICD-AT; n = 96), and pacemaker (PM, n = 84) patients. Mean age of all participants was 69 years; they were mostly male (62%) and married (75%). The final FPAS comprised 15 items with four consistent factors: Return to Function, Device-Related Distress, Positive Appraisal, and Body Image Concerns. RESULTS: The total FPAS demonstrated good internal consistency (Cronbach's alpha = 0.83), and internal consistency for each of the subscales ranged from 0.74 to 0.89. The FPAS demonstrated convergent, divergent, and discriminant validity when compared to other self-report measures of QOL, atrial symptoms, depression, and anxiety. A total FPAS score can be formed and between group comparisons with this sample indicated that ICD patients report a high level of acceptance (mean = 76), ICD-AT patients report a significantly higher level of acceptance (mean = 81.1), and PM patients reported the highest level of patient acceptance (mean = 85.4) of these implantable device groups. CONCLUSION: This initial psychometric investigation of the FPAS suggests that the FPAS may be useful in both clinical and research settings to assess patient acceptance of implantable cardiac devices.

Aged↗
PubMed1977

Evaluation of burn care in florida.

To determine if additional burn-care facilities or educational programs for burn care were needed within Florida, 73 of the state's 220 hospitals were studied. Data were collected on hospital statistics for a one-year period, as well as on 1656 patients admitted for burns to these hospitals during that year. When results were adjusted for severity of the injury, there was no evidence that outcomes of patients were better in any one or group of the State's eight regions, or in hospitals that had special burn units. Considerable evidence, however, indicated the need for additional educational programs in burn care. Many records were poor, admissions were inappropriate, patients with minor burns stayed too long, and burn shock was too frequent.

Adult↗