Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “DENTAL PULP”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 721 records · Page 40Linked to original sources
PubMed1983

The blood capillaries in the subodontoblastic region of the human dental pulp, as demonstrated by freeze-fracturing.

The microvasculature of the subodontoblastic region in the human dental pulp was studied, using freeze-fracturing. This technique allows an analysis of the fine structure of the vascular endothelium with special reference to the membrane structure. The blood capillaries were noted to be of the non-fenestrated or continuous type, although a few fenestrated vessels were observed. The endothelial plasmalemma often exhibited bundles of fibrillar structures, presumably myofilaments. There was a relatively large number of micropinocytotic vesicles and the interendothelial spaces were closed juxtaluminally by tight junctions (zonulae occludentes). The junctions appeared mostly as two to four strands, seen as ridges or grooves on the cell membrane. Thin-walled, irregular, tissue channels lacking the typical, blood-vessel configuration were disclosed. These vessels were believed to represent lymphatics.

Capillaries↗
PubMed1999

[Comparison of the effect of acetone-containing and acetone-less bonding materials on dental pulp blood vessels in rats].

No data are available on the direct acute vascular effect of dental bond materials in the dental pulp. The purpose of the present study was to investigate the effect of composite resin bonding systems on the pulpal vascular diameter. Three groups of male Sprague-Dawley rats (weighing 300-490 g), each of 10 animals were used for this investigation. The left lower incisor of the rats was prepared for vitalmicroscopy. Changes in vessel diameter were recorded prior to; and 5, 15, 30, 60 minutes after the application of saline (control) or the bonding agents (test1: acetone containing--/Prime/Bond2.1, DeTrey/; test2: acetone free /Scotchbond Multi-Purpose Adhesive System, 3M/ bond material) on pulpal dentin as recommended by the manufacturer. In control rats, the vessel diameter was stable during the experiment. However, in the presence of bonding materials an enhancement was registered in vascular diameter (p < 0.05). The bond materials applied directly onto a very thin layer of dentin show acute vasodilating effect on the rat pulpal microvessels, but no stasis or prestasis has been detected, indicating a possible reversible effect. We could not show any statistical difference between the vasodilatation caused by the acetone containing and the acetone free bonding material.

Acetone↗
PubMed1989

Response of the dental pulp to capping with a composite resin-bonded ceramic and dental adhesive in rat molars.

An inflammatory response occurs in the pulp tissue following the application of dental materials due to their chemical properties and/or a secondary bacterial irritation. Recently, a new line of composite resin products with reported improved esthetic qualities was designed to allow an intimate adherence with the dentin. This intense bonding could prevent bacterial penetration and marginal discoloration of these restorations. In addition, this method allows prevention of excessive removal of hard tissue. The present study examines pulpal reaction to capping with P-10 composite resin (Dental Products, USA) in rats. Histological examination did not reveal any inflammatory reaction in the nonexposed pulps. Direct pulp capping with P-10 induced formation of reparative dentin bridges.

Animals↗
PubMed1990

Detection of bacteria in histological sections of the dental pulp.

A series of deep cavities with and without traumatic pulpal exposures were prepared in the maxillary molars of albino rats. They were left untreated for various time intervals before killing. Demineralized sections were prepared in a mesiodistal plane and stained to demonstrate the presence of bacteria. In the cavities and coronal pulps that were grossly contaminated, bacteria were detected in the majority of sections. In the root canals and periapical tissues, bacteria were detected in only a minority of sections. It is considered that serial sections stained to demonstrate bacteria are necessary for the detection of bacteria that are present in only small numbers in the tissues. The demonstration of small numbers of micro-organisms in specific zones of the pulp remains problematic. Failure to detect stained bacteria in histological sections is not absolute proof of their absence.

Animals↗
PubMed1979

[Reactions of neurons in the dental projection area of the rabbit sensomotor cortex to photic, acoustic and painful stimulation of the dental pulp].

In experiments on unrestrained rabbits interactions of sound (tone, click), light and pain excitation occurring during stimulation of the dental pulp in the neurons of the cortical sensomotor zone, in the area of representation of the dental afferents were studied by the microelectrode method. It was shown that bi- and polysensor convergence of excitation is realized in the cells of this area. It is suggested that convergence of excitation underlies the mechanisms of formation of the integral systemic pain reaction during stimulation of the dental receptors.

Acoustic Stimulation↗
PubMed1998

Tube-like mineralization in the dental pulp of traumatized primary incisors.

Mineralization in the pulp is a common finding in permanent as well as primary teeth and is associated with caries, aging, traumatic injuries and systemic conditions. This article describes an unusual pattern of pulpal calcification. A tube-like calcified structure formed in the dental pulp of primary incisors following mild traumatic injuries. It was studied by clinical, radiographic and histologic evaluation and by scanning electron microscopy. The tube-like structure was found to extend along the entire length of the pulp canal. Generally it was separated from the root dentin by normal pulp tissue, but was connected to the dentin in some sites. It had the histologic appearance of osteodentin with cell inclusions in a ring-like formation that was incomplete in places. The scanning electron microscope study showed rough inner and outer surfaces of a tube-like structure with openings that seemed to be dentinal tubules.

Child↗
PubMed2003

Uptake and release of [3H]adenosine in human dental pulp.

The purpose of this study was to examine the uptake and release of adenosine in human dental pulp. Pulp was removed from extracted teeth and incubated in [3H]adenosine (0.02-3.2 micromol/l) for 30-90 min, washed for 30 min and the residual [3H]adenosine extracted and assayed. At 0.02 and 0.4 micromol/l the uptake was saturated after 30 min, whereas at 3.2 micromol/l it was not, even after 90 min. Destruction of the sympathetic nerves by 6-hydroxydopamine prior to incubation in [3H]adenosine (0.4 micromol/l) decreased the uptake of [3H] by 40%. Addition of S(4-nitrobenzyl)-6-thioguanosine (NBTG; 0.1 micromol/l), an inhibitor of adenosine uptake, blocked 90% of the uptake. Electrical stimulation of sympathetic nerves following incubation of pulp in [3H]adenosine (0.4 micromol/l) increased the overflow of [3H] into the medium superfusing the tissue segments. NBTG (0.1 micromol/l) had no effect on this overflow, whereas denervation with 6-hydroxydopamine prior to incubation decreased the overflow by 85%. Although the contribution of post-junctional elements, such as smooth muscle, to this release was not determined it is proposed that most of the purine released was derived from sympathetic nerves.

Adenosine↗
PubMed1997

Fine structure and distribution of lymphatic vessels in the human dental pulp: a study using an enzyme-histochemical method.

The existence of lymphatic vessels in the human dental pulp and their distribution were established by light and electron microscopy using an enzyme-histochemical method. The distinction between lymphatic and blood vessels was made by light microscopy on cryostat sections of undecalcified and decalcified teeth using 5'-nucleotidase(5'-Nase)-alkaline phosphatase double staining. On the tissue surface, 5'-Nase-positive lymphatic vessels were highlighted with good contrast and resolution by backscattered electron imaging using scanning electron microscopy. By transmission electron microscopy, dense granular precipitations resulting from the 5'-Nase reaction were seen on the luminal surface of the lymphatic endothelial cells as well as in the area at the basal side, but were absent in the blood vessels. These lymphatic vessels were more numerous in the central part than in the peripheral odontoblastic layer.

5'-Nucleotidase↗
PubMed1976

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro.

The time course for incorporation of KC-proline into various fractions of rabbit dental pulp in vitro has been measured. In the TCA-soluble precursor pool a steady state level of activity was indicated upon incubation after 3 h, whereas incorporation into protein and 14C-hydroxyproline, i.e. collagen formation, increased linearly for 9 h, leveling off upon further incubation. A lag period of about 3 h was indicated for the appearance of high molecular weight 14C-activity, including 14C-hydroxyproline, in the medium, increasing linearly from 3 h to the end of the incubation period (22 h). In this system, fluoride exhibited a dose-dependent inhibitory effect. At 5.3 mM fluoride the uptake of 14C-proline into the TCA-soluble pool was inhibited by about 50%, and the incorporation into protein and the subsequent conversion to hydroxyproline by about 90 and 60%, respectively. Release of collagen, i.e. 14C-hydroxyproline-containing material, seemed to be the process most sensitive to fluoride; it was inhibited by about 50% at the lowest concentration (1.3 mM) tested.

Animals↗
PubMed1998

Vascularity of the dental pulp after segmental osteotomy in the chacma baboon (Papio ursinus).

OBJECTIVE: To assess the vascularity of the dental pulp after segmental operations with and without interpositional autogenous bone grafting. DESIGN: Experimental study. SETTING: University Department, South Africa. ANIMALS: 26 chacma baboons. INTERVENTIONS: Maxillary and mandibular posterior segmental osteotomies were perfused with barium sulphate 3, 6, 12 and 18 months postoperatively. The animals were killed at 3, 6, 12 and 18 months after surgery and perfused with barium sulphate. Barium-filled vessels were counted in histological sections from 189 control and experimental teeth. MAIN OUTCOME MEASURE: Number of blood vessels. RESULTS: Blood vessel counts in mandibular teeth in osteotomy segments ranged from 0 to 1.15 compared with 2.27 to 4.58 in control teeth, while in maxillary teeth counts ranged from 0.54 to 2.22 for experimental teeth and 3.3 to 4.65 for controls. For both jaws, the numbers of vessels in experimental teeth gradually increased between 3 and 18 months but remained less than those in control teeth. Numbers of blood vessels were similar in graft and no-graft groups but both were less than half the counts in control teeth. CONCLUSION: Blood flow is present in the teeth at all times after posterior segmental osteotomy but there is a risk of ischaemia.

Animals↗
PubMed2006

Expression of toll like receptor 4 in normal human odontoblasts and dental pulp tissue.

The aim of the study was to determine the expression of TLR4 in odontoblasts and the dental pulp. Odontoblasts and pulp tissues were collected from freshly extracted human wisdom teeth. Reverse transcription-polymerase chain reaction and Western blotting were performed to detect TLR4 mRNA and protein expression, respectively. Immunohistochemical staining was used to determine the distribution of TLR4 in odontoblasts and the pulp. Scanning electron microscopy (SEM) was applied to observe the morphology of odontoblasts. It was demonstrated that TLR4 mRNA and protein expressions were both present in cells of odontoblast layer and pulp tissues and that TLR4 expression was distributed in odontoblasts and some pulpal vascular endothelial cells. SEM revealed the integrity of the odontoblast cell-layer and the well-preserved morphology of individual odontoblast cells. These findings suggest that TLR4 expressed in odontoblasts may play an important role in the dental immune defense.

Adolescent↗