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Procedures and equipment for staining large numbers of plant root samples for endomycorrhizal assay.

A simplified method of clearing and staining large numbers of plant roots for vesicular-arbuscular (VA) mycorrhizal assay is presented. Equipment needed for handling multiple samples is described, and two formulations for the different chemical solutions are presented. Because one formulation contains phenol, its use should be limited to basic studies for which adequate laboratory exhaust hoods are available and great clarity of fungal structures is required. The second staining formulation, utilizing lactic acid instead of phenol, is less toxic, requires less elaborate laboratory facilities, and has proven to be completely satisfactory for VA assays.

Botany↗

Soma of the Rigveda and an attempt to identify it.

Ephedra juice is used as the drink of longevity given even to the new born. This is an Aryan custom mentioned in Rigveda and followed even by the Romans. To trace the history of this custom would lead to identifying Soma = Ephedra. The original species of Ephedra or Soma proper would be Ephedra sinica, the Chinese plant. It is the one species with yellow stalks. This has been illustrated so that it confirms Rigveda speaking of Soma as "golden yellow." The name Soma is also a loan word from Chinese meaning "fire-yellow fibers of hemp." The plant Soma is described as "thousand boughs" and photographic evidence has been offered in support. Each stalk is rod-like resembling an arrow as Rigveda speaks of it. An illustration has been offered to show the plant with stalks which are straight and rod-like, comparable with an arrow.

Botany↗

Medicinal plants used for the treatment of hepatitis in Taiwan.

In an extensive ethnobotanical survey of the folk medicines of Taiwan, 69 kinds of crude drugs and 107 species of medicinal plants were found to be used for hepatitis treatments. The present paper is to update a list of medicinal plants used in the treatment of hepatitis and evaluate their liver protection by ethnopharmacological and pathological studies. Results show that some of these drugs have prominent therapeutic effects. Further investigation regarding the anti-hepatotoxic principles will be made.

Botany↗

Anatomical and histological studies of Bupleuri radix.

Three kinds of Bupleuri Radix were found in Taiwan. To clarify their origins, histological studies were carried out to identify their internal and external structures. It was found that Bupleuri Radix or "Chai-hu" of Taiwan is derived from roots of the following species" 1, Bupleurum chinense which was imported from China, 2, Bupleurum falcatum var. komarowi a cultivated production of Japanese origin, and 3, Bupleurum kaoi which is indigenous to Taiwan. These Bupleuri Radix were easily assigned by anatomical characteristics and their qualitative analysis of active principle - saikosaponin were established by TLC.

Anti-Inflammatory Agents, Non-Steroidal↗

Development of natural crude drug resources from Taiwan (X). Pharmacognostical studies on the Chinese crude drug "han-lian-cao".

In a previous paper(1), it was reported that the drug "Han-lian-cao", in Taiwan was derived from the entire plant or aerial part of Eclipta prostrata L. and Alternanthera sessilis R. Brown. In this paper, the histological studies of "Han-lian-cao" derived from Wedelia and Hypericum plants, and of 3 species of Wedelia related plants distributed in Taiwan were made. The results showed that it was possible to classify these species by their external and internal characteristics. In addition, a key to the characteristics of the whole plants of Eclipta, Alternanthera, Wedelia and Hypericum plants are hereby given.

Botany↗

Paving the path.

Fortuitous preparation and experiences led to the opportunity to use radioactive carbon dioxides to discern the path of carbon in photosynthesis. The search for the CO2 acceptor led to recognition of the growth stimulatory effect of methanol and its derivatives. With the techniques developed, radiochromatographic exploration led to discovery of major membrane lipids containing phosphorus, sulfur, and arsenic.

Botany↗

G. Ledyard Stebbins and the evolutionary synthesis.

More than any other individual, Stebbins synthesized knowledge from a disparate set of areas that included plant genetics, systematics, and evolution. This work culminated in 1950 with the appearance of his magnum opus, Variation and Evolution in Plants. This book gave plant evolution a coherent framework that was compatible with that emerging from the work of Theodosius Dobzhansky, Ernst Mayr, G. G. Simpson, and Julian Huxley, and others associated with establishing the synthetic theory of evolution. For this work he is regarded as the botanical "architect" of the evolutionary synthesis.

Biological Evolution↗