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PubMed2003

[Genetic variation in two forms of the common spadefoot toad Pelobates fuscus (Pelobatidae, Anura, Amphibia) distinguished by genome size].

Genetic differences (presumed 23 loci) between two cryptic forms of Pelobates fuscus, differing in genome size, were examined by means of polyacrylamide-gel electrophoresis. This method allowed to discriminate between these forms. Average genetic distance (DN) between both the forms was equal to 0.311, ranging from 0.055 to 0.563. As a rule, differences within these groups were smaller (0.021-0.388). The data show obvious genetic differentiation between these two cryptic forms of P. fuscus. Differences between these forms and P. syriacus were significantly higher (in average 0.943). Genetic distances in relation to speciation and species concepts are discussed.

Animals↗
PubMed1992

Primary olfactory terminations in the forebrain of amphibia: a comparative study with soybean agglutinin.

Ambystoma and Xenopus were used to investigate olfactory terminations in the forebrain by exploiting the selective binding of soybean agglutinin to primary olfactory projections. Lectin binding and tridimensional reconstruction provided new information on the distribution of olfactory glomeruli and on regional and interspecific differences. Ambystoma showed an uniform labelling of all olfactory terminations, which were localized in the rostro-lateral forebrain without obvious segregation of different terminal fields, except at dorsal levels. On the contrary, the binding pattern of Xenopus confirmed the existence of three sets of primary olfactory neurons characterized by clear differences in lectin binding. Selective soybean agglutinin binding and a detailed tridimensional reconstruction provided evidence for the existence of a set of neuronal projections, described for the first time, which terminate in a discrete bilateral region of the forebrain localized below the main olfactory bulb. The presence of three different sets of primary olfactory neurons, the experimental potentials and the possible functional significance of SBA binding sites are discussed.

Ambystoma mexicanum↗