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PubMed1978

Relationship between the rate of H+ transport and pathways of glucose metabolism by turtle urinary bladder.

The urinary bladder of the fresh-water turtle acidifies its contents by actively transporting H(+) ions across the luminal membrane. It is known that the H(+) transport system is dependent upon oxidative metabolism and the substrate glucose; however, the specific biochemical events resulting in H(+) translocation have not been identified. This study examines the relationship between active H(+) transport and a specific oxidative pathway of glucose metabolism, the pentose phosphate shunt. To investigate this relationship the metabolic and transport rates were simultaneously measured under several well-defined conditions. When H(+) transport was inhibited by either the application of an opposing pH gradient or by acetazolamide, glucose metabolism by the pentose phosphate shunt declined. Conversely, stimulation of H(+) transport by either imposing a more favorable pH gradient or by CO(2) addition resulted in an increase in pentose phosphate shunt metabolism. Glycolytic activity, in contrast, was invariant with the maneuvers which altered the rate of H(+) transport. Additional experiments localized pentose phosphate shunt enzyme activity to the mucosal fraction of the bladder which is the cell layer responsible for acid secretion. The finding that the rate of glucose metabolism by the pentose phosphate shunt is related to the rate of H(+) transport suggests but does not prove that the pentose phosphate shunt may be an important metabolic pathway for H(+) transport by the turtle urinary bladder.

Acetazolamide↗
PubMed1988

[Architectural ultrastructure of the urinary bladder epithelium. II. Changes in the urine-blood barrier in the contracted and distended state in the normal and inflammatory bladder].

The urinary bladder epithelium in mammals, including humans, has a low permeability to ions and small molecules such as sodium, urea and water. Two structures, asymmetrical luminal plasma membrane and tight junction between superficial cells, have been said responsible for the urine-blood barrier. The permeability of junctional complex between superficial cells to lanthanum was observed in rat urinary bladder epithelium by transmission electron microscopy (TEM). In the normal bladder epithelium, confirmed by bacteriological examination, most junctions between superficial cells are the tight junctions and 1 to 9% of the junctions are leaky. The lanthanum, known to penetrate the leaky junctions, is demonstrated in the intercellular space between intermediate and basal cells. This suggests that desmosomes between these cells have no barrier function. In the experimentally inflammatory bladder epithelium all junctions are tight and no leaky junction is found between superficial cells. In contrast, if the superficial cells were stripped off in the inflammatory change, the lanthanum penetrates the junction between denuded intermediate cells. In the normal bladder epithelium the structural junctions between superficial cells have no changes during contraction and distension. Thus this suggests that the permeability to lanthanum does not change during contraction and distension.

Animals↗
PubMed2006

Rho kinase: a target for treating urinary bladder dysfunction?

Urinary incontinence and other urinary storage symptoms are frequent in the general population but available treatments have limited efficacy and tolerability. Rho kinase (ROCK) has a central role in the regulation of smooth muscle contraction, including that of the urinary bladder. Recent experimental evidence indicates that this role could be deregulated and exacerbated in local and systemic pathological conditions that affect the bladder. In vitro studies with prototypical ROCK inhibitors such as Y27632 and in vivo data from animal models indicate that such drugs have potential as future treatments for bladder dysfunction.

Animals↗
PubMed1965

EFFECT OF ALKALI METAL CATIONS ON THE POTENTIAL ACROSS TOAD AND BULLFROG URINARY BLADDER.

Isolated urinary bladders of the bullfrog (R. catesbeiana) and the toad (B. marinus) were mounted in an Ussing chamber. Potential differences up to 114 mv were observed in bullfrog bladder when the mucosal surface was bathed in dilute Na(2)SO(4) and the serosal surface in sulfate Ringer's. In experiments with bullfrogs, K was used to replace Na in the mucosal solution and Na was used for K in the serosal solutions. The selectivity was judged in terms of the relative effectiveness of the replacement cation in maintaining the bladder potential. In experiments with toads, K and Rb were equally poor replacements for Na at the mucosal border, while Rb was a good replacement for K at the serosal border. Li in the mucosal solution appeared to depress the potential in part irreversibly. At the serosal border, Li was a partially effective substitute for K, more so than was Na. However, both were poor replacements compared to Rb. The mucosal surface of the urinary bladder of both frog and toad appears to be Na-selective and the serosal surface appears to be K-selective, consistent with the Koefoed-Johnsen-Ussing model for frog skin.

Animals↗
PubMedDate not supplied

[Spasmex treatment of patients with hyperactive urinary bladder].

Overactive urinary bladder (HAUB) is encountered in 16-20% patients with various voiding disorders. Etiologically, detrusor hyperactivity can be neurogenic and idiopathic. We studied efficacy of the antimuscarine drug spasmex in patients with imperative voiding disorders and compared its efficacy and tolerance with those of driptan and detrusitol. 193 female patients with imperative voiding disorders were examined. Spasmex given in a dose of 5 mg 3 times a day for 12 weeks produced a good effect in 56 patients: increased the effective capacity of the urinary bladder, reduced the number of voidings and episodes of incontinence for a day. A 3-month course of spasmex proved highly effective as shown by the patients' subjective response and positive changes in objective evidence. Being equally effective with detrusitol and driptan, spasmex is much superior to oxibutynin in safety.

Benzhydryl Compounds↗
PubMed1976

Effects of parathyroid hormone on H+ and NH+4 excretion in toad urinary bladder.

The urinary bladder of Bufo marinus excretes H+ and NH+4, and the H+ excretion is increased after the animal is placed in metabolic acidosis. The present study was done to determine if parathyroid hormone could stimulate the bladder to increase the excretion of H+ and/or NH+4. Parathyroid hormone added to the serosal solution in a final concentration of 10 mug/ml was found to increase H+ excretion by 50 per cent above the control hemibladders, while there was no effect on NH+4 excretion. Parathyroid hormone had no effect on H+ excretion when added to the mucosal solution. We also performed experiments utilizing theophylline and dibutyryl cyclic AMP which mimicked those of the parathyroid hormone experiments. A dose-response analysis was performed and the results indicate that 1 mug/ml of parathyroid hormone was the minimal effective dose. These results suggest that parathyroid hormone can stimulate H+ excretion in the toad urinary bladder and this effect seems to be mediated by cyclic AMP. In addition, it was found that parathyroid hormone has no effect on NH+4 excretion.

Ammonia↗
PubMed1989

[A case of leiomyoma of urinary bladder with relapse from the external urethral orifice--clinical analysis of leiomyoma of urinary bladder in Japan].

A 22-year-old female visited our hospital with complaints of pollakisuria and dysuria on July 3, 1987. Cystoscopy revealed a tumorous lesion in the urinary bladder. On July 8, 1987, she had urinary retention because of relapse of the tumor from the external urethral orifice. Under the diagnosis of urinary bladder tumor, tumorectomy was performed. The resected tumor had a steel, smooth surface, was elastic soft, red-purple and 7 g in weight. Pathological examination demonstrated the tumor to be composed of spindle-like cells, which showed no mitotic figures. Therefore, the tumor was diagnosed as leiomyoma of urinary bladder. In the literature, 67 cases of leiomyoma of the urinary bladder have been reported so far in Japan. The patients ages ranged from the 2nd to 8th decade, the peak age being the 4th decade. The sex ratio was 5 to 2, females being predominant. Chief complaints were hematuria, pollakisuria and dysuria, but prolapse of the tumor from the external urethral orifice was rare, only 3 cases including our case being reported thus far. Tumorectomy (34 cases, 27%) or partial cystectomy (16 cases, 27%) was performed in many cases, because it was benign. The prognosis was good, and there have been no reports stating that it become malignant.

Adult↗
PubMed1999

Superimposed histologic and genetic mapping of chromosome 9 in progression of human urinary bladder neoplasia: implications for a genetic model of multistep urothelial carcinogenesis and early detection of urinary bladder cancer.

The evolution of alterations on chromosome 9, including the putative tumor suppressor genes mapped to the 9p21-22 region (the MTS genes), was studied in relation to the progression of human urinary bladder neoplasia by using whole organ superimposed histologic and genetic mapping in cystectomy specimens and was verified in urinary bladder tumors of various pathogenetic subsets with longterm follow-up. The applicability of chromosome 9 allelic losses as non-invasive markers of urothelial neoplasia was tested on voided urine and/or bladder washings of patients with urinary bladder cancer. Although sequential multiple hits in the MTS locus were documented in the development of intraurothelial precursor lesions, the MTS genes do not seem to represent a major target for p21-23 deletions in bladder cancer. Two additional tumor suppressor genes involved in bladder neoplasia located distally and proximally to the MTS locus within p22-23 and p11-13 regions respectively were identified. Several distinct putative tumor suppressor gene loci within the q12-13, q21-22, and q34 regions were identified on the q arm. In particular, the pericentromeric q12-13 area may contain the critical tumor suppressor gene or genes for the development of early urothelial neoplasia. Allelic losses of chromosome 9 were associated with expansion of the abnormal urothelial clone which frequently involved large areas of urinary bladder mucosa. These losses could be found in a high proportion of urothelial tumors and in voided urine or bladder washing samples of nearly all patients with urinary bladder carcinoma.

Chromosome Aberrations↗
PubMed2004

Impact of changes in bladder and rectal filling volume on organ motion and dose distribution of the bladder in radiotherapy for urinary bladder cancer.

PURPOSE: To determine the impact of filling volume changes of the urinary bladder and rectum on organ motion and dose distribution of the bladder and rectum during radical radiotherapy for bladder cancer and to calculate the internal margins to secure target coverage. METHODS AND MATERIALS: In 15 patients with muscle-invasive bladder cancer, a planning CT scan was performed with a bladder and rectal catheter, followed by three immediate CT scans with various filling of the urinary bladder and rectum. After 20 fractions, a fifth CT scan, without catherization, was performed. In each CT study, the bladder and rectum volumes were delineated and matched to the planning CT scan to measure the organ motion and calculate internal margins. These margins were compared with an isotropic standard margin of 2 cm. Dose-volume histograms were analyzed to describe the dose distribution in the bladder and rectum corresponding to various filling volumes. RESULTS: Bladder movement was most pronounced in the anterior and cranial directions. The internal margins required to cover the bladder movements due to filling of the bladder and rectum in 87% of the patients were 2.4 cm in the anterior, 1.1 cm in the posterior, 3.5 cm in the cranial, 0.5 cm in the caudal, and 1.3 cm in the lateral direction. CONCLUSION: The filling volumes of the bladder and rectum have a large impact on bladder movements, especially in the anterior and cranial directions. This should be included in the internal target volume with the introduction of anisotropic margins in conformal radiotherapy for bladder cancer.

Aged↗
PubMed1994

Characterization and localization of epithelial Na+ channels in toad urinary bladder.

The toad urinary bladder and epithelial cell lines derived from the urinary bladder, including TBM, serve as model systems for the study of transepithelial Na+ transport. We examined biochemical characteristics of epithelial Na+ channels in toad urinary bladder and TBM cells and their cellular localization in the urinary bladder. The radiolabeled amiloride analogue [3H]benzamil bound to a single class of high-affinity binding sites in membrane vesicles from toad urinary bladder with a dissociation constant (Kd) of 10 nM. Photoactive benzamil analogues specifically labeled a 135,000-Da polypeptide in toad urinary bladder and TBM cells. A monoclonal anti-Na+ channel antibody directed against the amiloride-binding component of the channel specifically recognized a 135,000-Da polypeptide in TBM cells. Polyclonal anti-Na+ channel antibodies generated against purified bovine epithelial Na+ channel specifically recognized a 235,000-Da polypeptide in toad urinary bladder and localized Na+ channels to the apical plasma membrane of urinary bladder epithelial cells. The biochemical characteristics and the cellular localization of epithelial Na+ channels in toad urinary bladder are similar to those previously described in mammalian kidney and in the A6 cell line.

Amiloride↗
PubMed2005

Use of ultrasonography to identify infants for whom urinary catheterization will be unsuccessful because of insufficient urine volume: validation of the urinary bladder index.

STUDY OBJECTIVE: Frequently, attempts to obtain catheter urine samples from infants are unsuccessful because of an empty bladder, with urinary catheterization failure rates reported up to 16%. We seek to validate a sonographic urinary bladder index that will identify patients for whom catheterization will be unsuccessful. METHODS: We conducted a prospective, blinded, observational study enrolling a convenience sample of children younger than 2 years and undergoing urinary catheterization in an urban pediatric emergency department. Failure was defined as a catheterization result of less than 2 mL of urine. Urinary bladder index, a concept we created, is defined as the product of anteroposterior and transverse diameters, expressed in centimeters squared. Sensitivity and specificity were calculated with 95% confidence intervals (CIs). RESULTS: Forty-four patients were enrolled, with a median age of 8 months (range 0.5 to 24 months) and median weight of 7.8 kg (range 1.7 to 17.7 kg). Four children urinated during the procedure and were excluded. Thirty-five had urinary bladder index greater than 2.4 cm2 (range 2.5 to 15.5 cm2 ). All were successfully catheterized. Four children had urinary bladder index less than 2.4 cm2 (range 0 to 1.2 cm 2 ). No adequate samples were obtained from those children. The bladder was not visualized at all in 1 child who was successfully catheterized. Sensitivity of the urinary bladder index to identify failure to obtain 2 mL of urine was 100% (4 of 4; 95% CI 40% to 100%), specificity was 97% (35 of 36; 95% CI 85% to 100%). CONCLUSION: A urinary bladder index less than 2.4 cm2 appears to identify infants for whom urinary catheterization will be unsuccessful because of insufficient urine volume. Ultrasonographic evaluation with urinary bladder index measurement before catheterization may eliminate the incidence of failed procedures.

Female↗
PubMed1984

[Innervation of the rat urinary bladder. I. The morphological changes of intrinsic nerves of the urinary bladder after pelvic ganglionectomy].

The dual autonomic innervation of the urinary bladder of the female rat was studied using histochemical and light and electron microscopic methods. The bladder body in the normal state had a rich supply of cholinergic nerve fibers with a uniformly reticular pattern, but it was sparsely supplied with adrenergic nerves mainly associated with the blood vessels rather than the detrusor muscles. Seven days after the unilateral extirpation of the pelvic ganglion, most cholinergic and adrenergic fibers on the ipsilateral side of the bladder body disappeared markedly with the use of enzymatic and fluorescent histochemical techniques. The acetylcholinesterase activity of cholinergic fibers and the intensity of fluorescence of adrenergic fibers were somewhat restored 14 days after the operation. Seventy days postoperatively, it was found that the former had recovered in the relatively fine nerve fibers, while the latter had been almost completely restored, compared with the normal adrenergic innervation of the bladder body.

Adrenergic Fibers↗
PubMedDate not supplied

Expression of Thrombomodulin in the epithelium of the urinary bladder: a possible source of urinary thrombomodulin.

Thrombomodulin (TM) is an endothelial thrombin receptor which acts as a natural anticoagulant through inactivation of the procoagulant activity of thromin. In the present study, we demonstrated that TM is expressed on the urinary bladder epithelium. The cell line BOY established from human transitional cell carcinoma of the urinary bladder also expressed the TM message (3.7kb). These cells express an 85 kDa TM protein which is identical in size to that of MEG-01, a human megakaryoblastic cell line, reported previously. We also ascertained the expression and localization of TM in the human urinary bladder by immunohistochemical staining. TM was localized in the membranes of the transitional epithelium and the cytoplasmic region of umbrella cells. The expression of TM in the transitional epithelium increased with worsening pathological status of cystitis. Based on these results, we concluded that TM is expressed in the urinary bladder. Further, soluble TM in urine may be partially derived from the urinary bladder. At present, the physiological significance of TM expression in the urinary bladder remains to be elucidated.

Animals↗