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At least 73 records · Page 4Linked to original sources

Lead pollution in a large, prairie-pothole lake (Rush Lake, WI, USA): effects on abundance and community structure of indigenous sediment bacteria.

Rush Lake (WI, USA), the largest prairie-pothole lake east of the Mississippi River, has been contaminated with lead pollution as a result of over 140 years of waterfowl hunting. We examined: (1) the extent of lead pollution in Rush Lake sediments and (2) whether lead pollution in Rush Lake is affecting the abundance and community structure of indigenous sediment bacteria. Sediment lead concentrations did not exceed 59 mg Pb kg(-1) dry sediment. No relationship was observed between sediment lead concentration and the abundance of aerobic (P=0.498) or anaerobic (P=0.416) heterotrophic bacteria. Similarly, lead did not appear to affect bacterial community structure when considering both culturable and nonculturable community members. In contrast, the culturable fraction of sediment bacteria in samples containing 59 mg Pb kg(-1) exhibited a unique community structure. While factors other than lead content likely play roles in determining bacterial community structure in the sediments of Rush Lake, these data suggest that the culturable fraction of sediment bacterial communities is affected by elevated lead levels.

Bacteriological Techniques↗

Changing perspectives on the importance of urban phosphorus inputs as the cause of nutrient enrichment in Lough Neagh.

The scale and chronology of the phosphorus (P) enrichment of Lough Neagh, a large hypertrophic lake, was assessed using, as enrichment proxies, published palaeocological studies on diatoms and chironomid head capsules preserved in the lake sediments and, from 1974, monitoring data from the lake and its inflowing rivers. Enrichment commenced in 1880, and the scale and chronology of the diatom and chironomid records were similar up to 1960, but in the 1960s, chironomid accumulation rates increased dramatically, whereas diatom rates remained unchanged. From subsequent lake monitoring, the absence of a diatom response after 1960 was attributed to silica limitation so that P could be considered as the driver of increased diatom production only up to 1960. Using a coefficient for the demand for P by diatoms of 4 microg P mg SiO(2)(-1), it was calculated that the increased diatom production between 1881 and 1961 required an increase in lake P of 26 microg P l(-1). This value is close to the predicted increase of 22.5 microg P l(-1) in the lake caused by inputs of P from sewage treatment works and septic tanks. There was no evidence that diffuse source P contributed to enrichment over this period. Enrichment up to 1960 appears modest in comparison to subsequent years. From 1961 to 1974, lake P was estimated to have increased by 62 microg P l(-1), but only 25 microg P l(-1) of this increase was attributable to greater loadings of P from urban and septic tank sources. The enrichment response of chironomids at this time was also much greater than expected from a regression-derived relationship between urban P inputs and chironomid accumulation rates suggesting additional sources of P. The larger than expected increase in lake P was attributed to the onset of a significant internal loading of P and the commencement of an increase in diffuse source loadings of P. River monitoring has shown that diffuse P has increased steadily since 1974, more than counterbalancing a 40% reduction in urban P loadings that has taken place since 1980. By the end of the 20th century urban sources contributed only 19.7% of inflow P to Lough Neagh but lake P was 145 microg P l(-1) compared to an estimated concentration of 20 microg P l(-1) in 1881. Failure to control diffuse P sources has therefore been instrumental in the continued increase of lake P in Lough Neagh.

Animals↗

Temporal variation, dormancy, and coexistence: a field test of the storage effect.

Theoretical models suggest that overlapping generations, in combination with a temporally fluctuating environment, may allow the persistence of competitors that otherwise would not coexist. Despite extensive theoretical development, this "storage effect" hypothesis has received little empirical attention. Herein I present the first explicit mathematical analysis of the contribution of the storage effect to the dynamics of competing natural populations. In Oneida Lake, NY, data collected over the past 30 years show a striking negative correlation between the water-column densities of two species of suspension-feeding zooplankton, Daphnia galeata mendotae and Daphnia pulicaria. I have demonstrated competition between these two species and have shown that both possess long-lived eggs that establish overlapping generations. Moreover, recruitment to this long-lived stage varies annually, so that both daphnids have years in which they are favored (for recruitment) relative to their competitor. When the long-term population growth rates are calculated both with and without the effects of a variable environment, I show that D. galeata mendotae clearly cannot persist without the environmental variation and prolonged dormancy (i.e., storage effect) whereas D. pulicaria persists through consistently high per capita recruitment to the long-lived stage.

Journal Article↗

What minimal models cannot tell: a comment on "A model of phytoplankton blooms".

Huppert et al. analyzed a simple model describing bottom-up control of phytoplankton blooms. While we appreciate the mathematical aspects of this article, we feel that the fit of their model to data of Lake Kinneret is overly optimistic because the time axis was rescaled in an arbitrary way. Furthermore, one of the main conclusions of the article (bottom-up control is more likely to occur than top-down control) cannot, in our opinion, be drawn from a minimal model because such a model focuses on one explanation. It cannot be used to reveal the relative importance of alternative explanations.

Eutrophication↗

Early warming of tropical South America at the last glacial-interglacial transition.

Glaciation in the humid tropical Andes is a sensitive indicator of mean annual temperature. Here, we present sedimentological data from lakes beyond the glacial limit in the tropical Andes indicating that deglaciation from the Last Glacial Maximum led substantial warming at high northern latitudes. Deglaciation from glacial maximum positions at Lake Titicaca, Peru/Bolivia (16 degrees S), and Lake Junin, Peru (11 degrees S), occurred 22,000 to 19,500 calendar years before the present, several thousand years before the Bølling-Allerød warming of the Northern Hemisphere and deglaciation of the Sierra Nevada, United States (36.5 degrees to 38 degrees N). The tropical Andes deglaciated while climatic conditions remained regionally wet, which reflects the dominant control of mean annual temperature on tropical glaciation.

Journal Article↗

Biogeochemical evidence for dinoflagellate ancestors in the early cambrian

Dinoflagellates are single-celled organisms that reflect the ecological conditions in modern oceans and lakes. Ultrastructural data and molecular phylogeny suggest that they originated in the Neoproterozoic, yet dinoflagellate ancestors are classified only to the Middle Triassic ( approximately 240 million years ago). Examination of dinoflagellate-specific biological markers (dinosteranes and 4alpha-methyl-24-ethylcholestane) in concentrated microfossils with known morphology identified ancient dinoflagellate ancestors from the Early Cambrian ( approximately 520 million years ago).

Journal Article↗

Bacterial density in water determined by poisson or negative binomial distributions.

The question of how to characterize the bacterial density in a body of water when data are available as counts from a number of small-volume samples was examined for cases where either the Poisson or negative binomial probability distributions could be used to describe the bacteriological data. The suitability of the Poisson distribution when replicate analyses were performed under carefully controlled conditions and of the negative binomial distribution for samples collected from different locations and over time were illustrated by two examples. In cases where the negative binomial distribution was appropriate, a procedure was given for characterizing the variability by dividing the bacterial counts into homogeneous groups. The usefulness of this procedure was illustrated for the second example based on survey data for Lake Erie. A further illustration of the difference between results based on the Poisson and negative binomial distributions was given by calculating the probability of obtaining all samples sterile, assuming various bacterial densities and sample sizes.

Journal Article↗

Rates of mineralization of trace concentrations of aromatic compounds in lake water and sewage samples.

The rates of mineralization of phenol, benzoate, benzylamine, p-nitrophenol, and di(2-ethylhexyl) phthalate added to lake water at concentrations ranging from a few picograms to nanograms per milliliter were directly proportional to chemical concentration. The rates were still linear at levels of <1 pg of phenol or p-nitrophenol per ml, but it was less than the predicted value at 1.53 pg of 2,4-dichlorophenoxyacetate per ml. Mineralization of 2,4-dichlorophenoxyacetate was not detected in samples of lake water containing 200 ng of the chemical per ml. The slope of a plot of the rate of phenol mineralization in samples of three lakes as a function of its initial concentration was lower at levels of 1 to 100 mug/ml than at higher concentrations. In lake water and sewage supplemented with <60 ng of C-labeled benzoate or phenylacetate per ml, 95 to 99% of the radioactivity disappeared from solution, indicating that the microflora assimilated little or none of the carbon. The extent of mineralization of some compounds in samples of two lakes and sewage was least in the water with the lowest nutrient levels. No mineralization of 2,4-dichlorophenoxyacetate and the phthalate ester was observed in samples of an oligotrophic lake. These data suggest that mineralization of some chemicals at concentrations of <1 mug/ml is the result of activities of organisms different from those functioning at higher concentrations or of organisms that metabolize the chemicals at low concentrations but assimilate little or none of the substrate carbon.

Journal Article↗

Effects of chemical speciation on the mineralization of organic compounds by microorganisms.

The mineralization of 1.0 to 100 ng each of four complexing compounds-oxalate, citrate, nitrilotriacetate (NTA), and EDTA-per ml was tested in media prepared in accordance with equilibrium calculations by a computer program so that the H, Ca, Mg, Fe, or Al complex (chemical species) was predominant. Sewage microorganisms mineralized calcium citrate more rapidly than iron, aluminum, or hydrogen citrate, and magnesium citrate was degraded slowest. Aluminum, hydrogen, and iron oxalates were mineralized more rapidly than calcium oxalate, and magnesium oxalate was decomposed slowest. Sewage microorganisms mineralized calcium NTA but not aluminum, magnesium, hydrogen, or iron NTA or any of the EDTA complexes. Pseudomonas sp. mineralized calcium and iron citrates but had no activity on hydrogen, aluminum, or magnesium citrate. Pseudomonas pseudoalcaligenes mineralized calcium, iron, hydrogen, and aluminum citrates but had little activity on magnesium citrate. Pseudomonas alcaligenes used calcium, iron, hydrogen, and aluminum oxalates readily, but it used magnesium oxalate at a slower rate. Listeria sp. destroyed calcium NTA but had no effect on hydrogen, iron, or magnesium NTA. Increasing the Ca concentration in the medium enhanced the breakdown of NTA by Listeria sp. The different activities of the bacterial isolates were not a result of the toxicity of the complexes or the lack of availability of a nutrient element. NTA mineralization was not enhanced by the addition of Ca to Beebe Lake water, but it was enhanced when Ca and an NTA-degrading inoculum were added to water from an oligotrophic lake. The data show that chemical speciation influences the mineralization of organic compounds by naturally occurring microbial communities and by individual bacterial populations.

Journal Article↗

PheBee: A Graph-Aware System for Scalable, Traceable, and Semantic Phenotyping.

OBJECTIVES: Phenotype-driven workflows in clinical and translational research require standardized ontology-based representation, ontology-aware cohort discovery, and provenance inspection for each assertion. Existing approaches optimize either for semantic traversal or scalable batch analytics, but not both. We describe PheBee, a hybrid system that links semantic assertions to scalable evidence storage via a deterministic identifier, preserving provenance while supporting ontology-aware discovery at cohort scale. MATERIALS AND METHODS: PheBee represents phenotype assertions in a knowledge graph as ontology-linked nodes with clinical modifier context (e.g., negated, family history), and stores supporting evidence records in a scalable row-oriented evidence table for cohort-scale access. The two layers are connected by a deterministic identifier enabling stable joins across repeated ingestions without duplicating high-volume evidence in the graph. We evaluated PheBee using synthetic datasets designed to exercise end-to-end ingestion and query workflows. RESULTS: Functional evaluation validated hierarchical term expansion, qualifier-aware retrieval, duplicate-free assertion handling under re-ingestion, and privacy-conscious management of subjects shared across multiple research projects. At scale (10,000 subjects producing 12M evidence records) PheBee completed ingestion in ~30 minutes and responded to interactive queries within 6 seconds under concurrent load. DISCUSSION: PheBee exposes a unified API for ontology-aware cohort discovery with hierarchical term expansion, subject-centric retrieval of phenotypes and clinical modifiers, and evidence and provenance queries. Its data model aligns with GA4GH Phenopackets, facilitating interoperability with phenotype exchange standards. CONCLUSION: By combining ontology-aware semantics with scalable, provenance-bearing evidence storage, PheBee provides a practical open-source foundation for phenotype-driven research workflows that demand both semantic precision and cohort-scale traceability.

cohort studies↗

[Microsporidians, cnidosporidians and ciliatae (Peritricha: Peritrichida: Mobilina), found in fishes from water bodies of the Minusinsk region].

During the period of 20 years (1964-1984) 10,000 fish specimens collected in water bodies of the Minusinsk depression have been examined by the methods of total and partial parasitological dissections. The studies were held in the Enisey, Abakan, Tasheba, and Tuba rivers, in the Krasnoyarskoye and Sayano-Shushenskoye reservoirs, and in some lakes. The data on a parasite species composition of microsporidians, cnidosporidians and ciliatae are given.

Animals↗

Periphyton: a primary source of widespread and severe taste and odour.

In the last decade, a late summer-fall taste and odour problem has been a prolonged and annual event in the St Lawrence River (SLR). Earlier work identified the earthy/musty compounds geosmin and particularly, 2-methylisoborneol (GM-MIB), and ruled out Lake Ontario as a major source, but did not identify the biological origins. In 2000, we investigated the source(s) and underlying causes. We sampled littoral sites in the SLR near Cornwall, ON, and found that macrophytes (or associated biofilms) may be primary GM sources. Zebra mussel homogenate yielded low GM-MIB levels, but several associated actinomycetes generated high in vitro amounts. Periphyton from rocks showed significant yields, with cell-bound GM-MIB up to one hundred times the levels in overlying water. In 2001, we followed seasonal changes at some of these sites. Periphyton GM-MIB showed intriguing spatial and temporal patterns. Several cyanobacteria in these biofilms were identified as potential odour sources, notably Oscillatoriales. We conclude: i) periphyton is a major odour source in the SLR; ii) other biota such as macrophytes and mussels may also contribute; iii) seasonality in GM-MIB production and ratios indicate changes in cell production and/or taxa in response to environment. These results may account for the recent onset of the problematic odour events, which represent chemical signals of the increased water transparency and littoral surface area following the widespread dreissenid mussel invasion to the Great Lakes. Our data raise key questions about the processes that trigger the tremendous variability in biota and GM-MIB production in the SLR, the subject of our continued research.

Biofilms↗

[Schistosoma japonicum in the newly identified areas in Hunan province].

OBJECTIVE: To study the major risk factors and characteristics of Schistosoma japonicum in the newly identified areas around the Dong Ting Lake. METHODS: Data was collected regarding the distribution of snails, prevalence of infection as well as the source of transmission in the newly identified areas in Hunan province from 1979 to 2003. RESULTS: In all the newly identified sites, Schistosomiasis japonica patients appeared to be the first-seen evidence, some even under acute phase. The highest infection rate among residents had reached 87.1% in the newly identified villages. Most of the new endemic areas concentrated in the ditch and beach areas that close to cities and towns. CONCLUSIONS: Evidence showed that snails had been transferred through boats and fishing nets to be disseminated. Data also revealed that the sources of transmission would include those local infected residents who had been infected in the old endemic areas and the newly imported domestic animals with infection.

Adolescent↗

[The circulation of the California encephalitis complex and Batai viruses in the Lake Baikal region (serological research data)].

The serological materials of the indigenous population and cattle suggest that there are viral foci of a complex of California encephalitis with the circulation of viruses of Inkoo, Tahyna [correction of Tyagin], and the white hare. The results of examining the residents of Irkutsk also suggest that there are urban viral foci of a complex of California encephalitis. The epidemiological potential of the natural foci of Batai virus is insignificant in the Baikal region.

Animals↗

Assessment of chlorophyll-a concentration and trophic state for Lake Chagan using Landsat TM and field spectral data.

In this study, we present the digital evaluation of Landsat TM data and field spectral measurements for retrieving chlorophyll-a (chl-a) concentration and trophic state index in Lake Chagan of Northeast China. Chl-a concentration of the lake can be estimated from the band ratio (TM4/TM3) and the field spectral data at 670 nm (absorption peak) and at 700 nm (reflectance peak). The results show that the best determination coefficient (R (2)) is 0.67 from the TM data, by which chl-a distribution can be mapped. Based on chl-a determination from laboratory analysis, field spectral and TM data, the modified trophic state index (TSI(M)) was applied to assess the lake's trophic state. With the available data in Lake Chagan, each algorithm demonstrates the similar result for assessing the lake's chl-a and trophic state. Our results indicate that Landsat TM and field spectral data could be used effectively to determine chl-a concentration and evaluate the trophic state of Lake Chagan in the study.

Algorithms↗

[Long-term production dynamics of aquatic vegetation in the Arakhlei Lake (Eastern Transbaikalia)].

Long-term data on aquatic vegetation production in the mesotrophic Arakhlei Lake was comparatively analyzed. The results demonstrated that the production dynamics of aquatic vegetation communities depend on the water-level conditions of the lake. The data on the succession of dominant vegetation communities are given: Chara sp. and Ceratophyllum demersum --> C. demersum and Lemna trisulca. At the same time, the increased proportion of C. demersum and L. trisulca pointed to an increased anthropogenic stress on the lake.

Araceae↗