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At least 73 records · Page 4Linked to original sources
PubMed2005

Biostimulation of dermal fibroblast by sublethal Q-switched Nd:YAG 532 nm laser: collagen remodeling and pigmentation.

The application of medical lasers in treating pigmented lesions has rapidly developed over the past decade. In both clinical and cosmetic application, melanin is targeted in pigmented areas and destroyed by the mechanism of selective photothermolysis. When laser radiation passes through superficial pigmented tissue, energy will be further reduced by dermal collagen scattering and absorption. Non-pigmented dermal fibroblasts will be exposed to co-incidental laser irradiation at lower energy levels. Biostimulation of dermal fibroblasts by low energy laser is reported in this paper. The Q-switched frequency doubled Nd:YAG 532nm laser used in clinical laser therapy was used in this study. Sublethal laser fluence was determined at 0.8J/cm(2) and used to stimulate normal human fibroblasts in monolayer culture. The results showed that there was no significant difference in collagen synthesis between the stimulated fibroblasts and controls. However, significant delay in collagen remodeling activity was demonstrated in the irradiated group by measuring fibroblast populated collagen lattice (FPCL) contraction. The stimulation of SCF, HGF and b-FGF gene expression was determined by RT-PCR analysis and demonstrated to vary between cases. Two out of six cell lineages that showed stronger responses to laser stimulation on SCF, HGF and b-FGF gene expressions were used to prepare conditioned media. The conditioned media from irradiated groups showed significant increase in SCF and b-FGF content and stimulated SK-mel-3 melanoma cells to synthesize more melanin in vitro. These results suggest that sublethal laser stimulation of fibroblasts may cause post-laser hyperpigmentation through production of melanogenic stimulatory cytokines. The degree of stimulation of SCF, HGF and b-FGF production varied between individual cell lineages, which may reflect the true variation of post-laser hyperpigmentation in clinical practice.

Adult↗
PubMed2004

Effect of biostimulation on uterine involution, early ovarian activity and first postpartum estrous cycle in beef cows.

The objective was to determine the effect of biostimulation (bull-exposure) on uterine involution (UI), plasma progesterone concentration (P4), size of largest follicle (LF), number of follicles larger than 5 mm ( F > or = 5 ), presence of fluid in uterine lumen (PF), presence of luteal tissue (LT), and length of the first estrous cycle postpartum (LEC). Ninety Angus cows with calves were allocated by parity and body frame into three groups (30 per group) 1 week postpartum. Two groups were exposed to bulls (BE) and one non-exposed group (NE) served as a control. Data were collected during weekly sessions of palpation per rectum, ultrasonography and bleeding on a subgroup of 30 cows (10 cows per group) for 6 weeks, and permanent surveillance of estrus with HeatWatch on all 90 cows. There were no significant differences between BE and NE cows for UI ( 17.1+/-1.1 days versus 20.1+/-1.6 days), LF ( 9.5+/-1.7 mm versus 11.0+/-2.4 mm), F > or = 5 ( 1.20+/-0.3 versus 1.47+/-0.09 ), and PF. However, LT was detected in more BE than NE cows (13 versus 2; P<0.001 ). Overall differences in P4 were found between BE and NE cows with detected LT ( 2.00+/-0.3 ng/ml versus 1.05+/-0.4 ng/ml, respectively; P<0.05 ). More BE cows resumed reproductive cyclicity with estrous cycles normal in length compared with NE cows (16/30, 53%; 16/30, 53%; and 8/30, 26.6%, for the two BE groups and the NE group, respectively; P<0.01 ). In conclusion, BE hastened luteal function but did not affect uterine involution.

Animals↗
PubMed2001

Biostimulation-based bioremediation of diesel fuel: field demonstration.

Ex-situ bioremediation of leached cynamonic forest soil at initial diesel oil contamination of 6,000 mg kg(-1), 4,000 mg kg(-1) and 2,000 mg kg(-1) was investigated after biostimulation with inorganic fertilizers. It was found that the added nutrients had no effect on the decontamination of polluted soils. A precise and reliable approach for evaluation of the biodegradation process is proposed. It comprises application of sensitive and easily accessible diagnostic parameters and relations, calculated on the basis of n-alkanes and isoprenoids--pristane (2.6.10.14-tetramethylpentadecane, i-C19H40) and phytane (2.6.10.14-tetramethylhexadecane, i-C20H42) distribution.

Alkanes↗
PubMed1987

Biostimulation of wound healing in vivo by a helium-neon laser.

Clinical observations have suggested that low-energy lasers might stimulate wound healing. To understand the mechanism of the biostimulation, we previously examined the effects of low-energy lasers on collagen production by human skin fibroblasts and reported an increase of collagen synthesis in vitro. To examine the effects of low-energy lasers in vivo, hairless mice were experimentally wounded, sutured, and subjected to laser irradiation by a helium-neon laser with a power output of 1.56 mW and an energy fluence of 1.22 Joules/cm2. Experimental wounds were subjected to laser treatment every other day for 2 months; control wounds remained untreated. Specimens from the wounds were then examined for histological findings, tensile strength, and total collagen content. Results demonstrated a considerable improvement in the tensile strength of the laser-irradiated wounds at 1 and 2 weeks. Furthermore, the total collagen content was significantly increased at 2 months when compared with control wounds. These results suggest a beneficial effect of the helium-neon laser on wound healing in vivo.

Animals↗
PubMed2004

Biostimulation of micro-organisms from sugarcane bagasse pith for the removal of weathered hydrocarbon from soil.

AIMS: In this study we studied the biostimulation of micro-organisms associated with sugarcane bagasse pith for the removal of total petroleum hydrocarbon from a soil contaminated with weathered hydrocarbon. METHODS AND RESULTS: Carbon, nitrogen and phosphorus were added at a ratio of 100 : 10 : 1, water content of 40%, and soil : bagasse ratio of 49 : 1. A significant positive difference (P < 0.05) was observed in total petroleum hydrocarbon removal (38 and 48%) by micro-organisms associated with bagasse and native soil micro-organisms, respectively. In addition, total petroleum hydrocarbon removal increased to 60% in a system where both autochthonous soil and bagasse micro-organisms were present. CONCLUSIONS: Micro-organisms from sugarcane bagasse pith can be stimulated for removal of weathered hydrocarbon from contaminated tropical soils, without they being inhibited by indigenous soil micro-organisms. SIGNIFICANCE AND IMPACT OF THE STUDY: Soil of with hydrocarbons can be diminished by stimulation of autochthonous microflora present in soil and agricultural residues. This work contributes to the microbiology of composting, as low amounts of bulking agents for hydrocarbon removal from soil, can be used.

Bacteria↗
PubMed1988

Biostimulation of human carcinoma cells with the argon laser: a previously unreported potential iatrogenic effect of lasers.

The human squamous carcinoma cell line P3 was subjected to treatment with a single mode argon laser at 514.5 nm. The temperature and energy levels delivered to the target cells were determined by a reproducible method of dosimetry. At energy levels between 860 to 990 J/cm2 and a corresponding temperature of 39 +/- 1 degrees C, a significant delayed stimulation in DNA synthesis was noted after 24 hours, but the cells remained viable. However, at energy levels and temperatures higher or equal to 1100 J/cm2 (41 degrees C), an immediate suppression of DNA synthesis was accompanied by nonviability of the P3 carcinoma cells. These results indicate that the argon laser has potential for selective biostimulation on carcinoma cell duplication at the specific "non-thermal" range of 39 +/- 1 degrees C. Similar effects were not observed when the P3 carcinoma cells were heated to this same temperature using a standard heat bath. This phenomenon appears to represent a previously undescribed potential iatrogenic effect of the monochromatic laser beam in the treatment of cancer.

Carcinoma, Squamous Cell↗
PubMed2005

Comparison of biostimulation versus bioaugmentation with bacterial strain PM1 for treatment of groundwater contaminated with methyl tertiary butyl ether (MTBE).

Widespread contamination of groundwater by methyl tertiary butyl ether (MTBE) has triggered the exploration of different technologies for in situ removal of the pollutant, including biostimulation of naturally occurring microbial communities or bioaugmentation with specific microbial strains known to biodegrade the oxygenate. After laboratory studies revealed that bacterial strain PM1 rapidly and completely biodegraded MTBE in groundwater sediments, the organism was tested in an in situ field study at Port Hueneme Naval Construction Battalion Center in Oxnard, California. Two pilot test plots (A and B) in groundwater located down-gradient from an MTBE source were intermittently sparged with pure oxygen. Plot B was also inoculated with strain PM1. MTBE concentrations up-gradient from plots A and B initially varied temporally from 1.5 to 6 mg MTBE/L. Six months after treatment began, MTBE concentrations in monitoring wells down-gradient from the injection bed decreased substantially in the shallow zone of the groundwater in plots A and B, thus even in the absence of the inoculated strain PM1. In the deeper zone, downstream MTBE concentrations also decreased in plot A and to a lesser extent in plot B. Difficulties in delivery of oxygen to the deeper zone of plot B, evidenced by low dissolved oxygen concentrations, were likely responsible for low rates of MTBE removal at that location. We measured the survival and movement of strain PM1 in groundwater samples using two methods for detection of DNA sequences specific to strain PM1: TaqMan quantitative polymerase chain reaction, and internal transcribed spacer region analysis. A naturally occurring bacterial strain with > 99% 16S rDNA sequence similarity to strain PM1 was detected in groundwater collected at various locations at Port Hueneme, including outside the plots where the organism was inoculated. Addition of oxygen to naturally occurring microbial populations was sufficient to stimulate MTBE removal at this site. In some cases, however, inoculation with an MTBE-degrading culture may be warranted.

Bacteria, Aerobic↗
PubMed1987

Influence of biostimulation by mature bulls on occurrence of puberty in beef heifers.

The objective of this study was to determine if biostimulation of prepuberal beef heifers by mature bulls would alter proportions of heifers exhibiting puberty, or age or weight at puberty. Angus (A), A X Hereford (H) and Tarentaise X HA heifers (n = 103) were stratified by age and weight within breed-type and location of birth and allotted randomly to the following treatments: 1) heifers exposed to mature bulls (T1; n = 52) or 2) heifers isolated from bulls (T2; n = 51). At the start of the experiment, heifers in T1 and T2 were 287 +/- 2 and 286 +/- 2 d of age, respectively. Male-to-female ratio for T1 was 1:26. Heifers in T1 and T2 were maintained in drylots separated by .5 km. Heifers were observed for estrus twice daily for 152 d. Puberty was characterized by the following criteria: 1) behavioral estrus, 2) presence of a palpable corpus luteum (d 9; estrus = d 0) and 3) a rise in serum progesterone above 1 ng/ml (d 9). Proportions of heifers reaching puberty by 11, 12, 13, 14 and 15 mo of age did not differ (P greater than .10) between treatments. Percentages of heifers reaching puberty by the end of the experiment were 84 and 89% for T1 and T2, respectively. Age and weight at puberty did not differ (P greater than .10) between treatments and averaged 370 +/- 7 d and 293 +/- 4 kg, respectively. Results from this experiment indicated that presence of mature bulls did not alter proportions of beef heifers reaching puberty, or age and weight at puberty.

Animals↗
PubMed1999

An ultrastructural study of the biostimulative effect of He-Ne laser radiation on the early postnatal differentiation of the rat submandibular gland.

Submandibular gland of the rat during the first week of postnatal development was used for the study of the ultrastructural changes after the He-Ne laser radiation. In the experimental group we observed an increase of the dark cells with greater quantity of the granular endoplasmic reticulum and an increased number of specific secretory granules. These changes show positive biostimulative effect of He-Ne laser radiation on the morphologic differentiation of the submandibular gland of the rat in the early postnatal period.

Aging↗
PubMed2000

[Use of transcutaneous laser biostimulation of blood and a running alternating magnetic field in preparing periodontitis patients for surgery].

A total of 103 patients with exacerbation of chronic generalized periodontitis of moderate and high severity were treated using running alternating magnetic field generated by ATOS device and transcutaneous laser biostimulation of the blood. These treatment modalities accelerated preoperative treatment and allowed performing the operations on the periodontal tissues in the optimal status under conditions of improved defense forces of the organism.

Adolescent↗
PubMedDate not supplied

[Effect of antibacterial factors and cell metabolism biostimulants on bacterial luminescence].

Bioluminescence was used as an index of effects of a series of factors (differing in origin and structure) on cell metabolism. Luminescence of intact cells of Escherichia coli lum+ (a genetically modified strain) was measured on exposure to antibiotics, probiotics, phages, and biostimulators. The sensitivity of E. coli lum+ to antibiotics correlated with the antibiotic-induced luminescence quenching. Bioluminescence-based assessment of the antagonistic activity of the main probiotics (bificoll, bifidobacterin, acylact, colibacterin, and a composite probiotic preparation), each taken at a concentration of 1 dose/ml demonstrated that bacterial luminescence was inhibited by 75-99.9% (exposure time, 30 min). Bioluminescence changes reflected cell damage associated with phage infection. It was shown that bioluminescence stimulation could be used as an index of the effect of immunomodulators (olexin and vermin) on cell metabolism in bacterial cultures.

Adjuvants, Immunologic↗
PubMed2005

Enhancing the intrinsic bioremediation of PAH-contaminated anoxic estuarine sediments with biostimulating agents.

Estuarine sediments are frequently polluted with hydrocarbons from fuel spills and industrial wastes. Polycyclic aromatic hydrocarbons (PAHs) are components of these contaminants that tend to accumulate in the sediment due to their low aqueous solubility, low volatility, and high affinity for particulate matter. The toxic, recalcitrant, mutagenic, and carcinogenic nature of these compounds may require aggressive treatment to remediate polluted sites effectively. In petroleum-contaminated sediments near a petrochemical industry in Gwangyang Bay, Korea, in situ PAH concentrations ranged from 10 to 2,900 microg/kg dry sediment. To enhance the biodegradation rate of PAHs under anaerobic conditions, sediment samples were amended with biostimulating agents alone or in combination: nitrogen and phosphorus in the form of slow-release fertilizer (SRF), lactate, yeast extract (YE), and Tween 80. When added to the sediment individually, all tested agents enhanced the degradation of PAHs, including naphthalene, acenaphthene, anthracene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, and benzo[a]pyrene. Moreover, the combination of SRF, Tween 80, and lactate increased the PAH degradation rate 1.2-8.2 times above that of untreated sediment (0.01-10 microg PAH/kg dry sediment/day). Our results indicated that in situ contaminant PAHs in anoxic sediment, including high molecular weight PAHs, were degraded biologically and that the addition of stimulators increased the biodegradation potential of the intrinsic microbial populations. Our results will contribute to the development of new strategies for in situ treatment of PAH-contaminated anoxic sediments.

Anaerobiosis↗
PubMed1990

[Biostimulation with low-intensity lasers--physics or metaphysics?].

The use of low-intensity lasers for biostimulation continues to produce controversies. This paper presents the basic principles of light propagation in tissue. Tissue properties such as fluorescence, absorption and scattering initiate a gradual loss of any "laser-specific" property of the light with distance from the irradiated surface. One of the aims of the discussion is to indicate if a given biological response is expected to be specifically dependent on a laser source, or if the same result also ought to be expected from more conventional light sources.

Biophysical Phenomena↗
PubMedDate not supplied

[Ultrastructure of the adrenal glands in rats under stress after preliminary use of the biostimulator "Biomos"].

Adaptogenic influence of biostimulator "Biomos" on the course of stress-reaction in adrenal glands has been shown by the electron-microscopic method. It consists in essential reconstructions of steroidogenesis apparatus directed to potentiation of mineralocorticoid function and to stabilization of the glucocorticoid function as well as to maintenance of structural integrity of substantia medullaris.

Adaptation, Physiological↗
PubMed1986

[Effect of the E-50 Biostimulant on sperm production in young bulls].

The Biostimulant E-50 preparation, a biogenic stumulant, was applied to 8 bulls divided into two groups--with normal and with disturbed semen production (lower qualitative indices of semen and higher number of discarded ejaculates). The stimulant was injected muscularly at the rate of 40 cu. cm, twice, at a seven-day interval, with one of the bulls it being used in a second series. Studied were a total of 587 ejaculates taken from the test bulls following treatment. It was found that the agent produced a favourable effect on semen production, the reaction being individual and better manifested with bulls having disturbances. Up to 13.7 per cent of the ejaculates of bulls with normal semen production were discarded, and up to 31.8 per cent of those showing signs of disturbed spermiogenesis. The spermogram indices with some bulls reliably rose (P less than 0.01 and 0.05) in terms of concentration and motility, the number of dead spermatozoa coming back to normal at negligible rise of the pH value.

Animals↗
PubMed1979

[Nutritive antibiotics and biostimulant preparations in the rations of swine for fattening].

Two experiments were carried out with sows for fattening having a body weight of 20 to 100 kg. The following nutritive antibiotics were tested -- bacipharmin -- 10% (PRB), zinkbacitracin -- 10% (UFRJ), vitamycin 3 (USSR), nitrovin (Peizun, USA) and mekadox -- 5% (USA). It was established that the stimulating effect of these antibiotics was manifested during the first fattening period -- up to 60 kg body weight, when the average daily increment of the experimental groups treated with bacipharmin, zink bacitracin and nictrovin in the I experiment was 7--15% higher and in the II experiment in which zink bacitracin, vitamycin 3, nitrovin and mekadox were added -- 8--14% higher as compared to the control groups. During the second period of fattening (60--100kg) no effect of the same nutritive antibiotics was observed, with the exception of the vitamycin treated group. In both experiments an economy of combined fodder per 1 kg body weight was realized in the experimental groups. Application of nutritive antibiotics and biostimulators during the second period is economically unexpedient. The antibiotics tested have no negative effect on carcass slaughter characteristics and on the chemical composition of meat. No residual substances of the nutritive antibiotics tested were detected on the second day following slaughter in the meat group treated with mekadox, since no sows of this group were slaughtered.

Animal Feed↗
PubMed1996

Mechanisms of biostimulating effects of therapeutic laser in vivo. Review article.

The beneficial biostimulating effects induced by therapeutic laser in involved tissues was checked by a number of experimental as well as clinical works. Most of them particularly pay their attention to the description of the basic disease; there is, however, considerable imprecision in the technical characterization of the laser appliances used. Only the wave length and the power of the source are typically characterized. Further principal data are, however, missing, such as the power density, energy density, etc. These communications also suggest that their authors are not always completely familiarized with the mechanism of the stimulating action low-power laser beams in tissues. Thus, the purpose of the present communication is to summarize the existing knowledge concerning this type of treatment with considering possibilities of its use in clinical practice.

Animals↗