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At least 73 records · Page 4Linked to original sources
PubMed1999

Uptake and retention of Vibrio cholerae non-O1, Salmonella typhi, Escherichia coli and Vibrio harvey by mussels in seawater.

Vibrio cholerae non O1 is known to persist in estuarine and freshwater environments. Experiments evaluated the amount of microorganisms accumulated in mussels maintained in static seawater, contaminated with 10(4) to 10(6) cells/ml and the depuration time required in circulating water. Accumulation and retention times were compared with those for Escherichia coli, Salmonella typhi and Vibrio harvey. E. coli and S. typhi accumulated to a greater extent and were released from mussels more quickly than vibrios which became undetectable 2 to 3 days later than E. coli. Seasonal seawater temperatures (14 to 21 degrees C) had a limited influence on depuration but vibrios appear to be retained with more efficacy over 16 degrees C while E. coli and S. typhi were eliminated to a greater extent. When mussels were contaminated with mixed culture, vibrios appeared to predominate on E. coli, while no interference was observed between E. coli and S. typhi.

Animals↗
PubMed1999

[DNA polymorphism of Vibrio cholera and other vibrios].

OBJECTIVE: To investigate the inherent characteristics of V. cholera and other vibrios and the association between them. METHODS: Polymerase chain reaction (PCR), DNA sequences analysis, randomly amplified polymorphic DNA (RAPD) and average linkage cluster analysis were used to study V. cholera including 3 strains of O139, 4 strains of E1 Tor biotype, 3 strains of classical and 3 other vibrios. RESULTS: V. cholera O139 contained the genomic sequences of CTX A2-B as well as O1 V. cholera, indicating that their homologous nucleotides was 96.9%-98.7%, and other vibrios did not contain CTX. O1 V. cholera and other vibrios were divided into 4 groups by RAPD: classical; O139 and E1 Tor; V. paraheamolyticus and V. vulnificus; and V. flluvialis. The O139 DNA fingerprints of RAPD were almost as same as E1 Tor biotype, whose average linkage cluster distance was 0, similar with classical biotype, whose average linkage cluster distance was 2.07, much different from V. paraheamolyticus and others with the average linkage cluster distance being 6.76-8.54. CONCLUSION: V. cholera and other vibrios are polymorphic in inherent characteristics. Being as same as the inherent characteristics of E1 Tor, O139 may evoluted from E1 Tor biotype. And also the inherent characteristics of V. parahemolytisus were as same as those of V. vulnificus.

DNA, Bacterial↗
PubMed1994

[Extraintestinal infections caused by Vibrio parahaemolyticus and Vibrio alginolyticus at the county of Funen 1987-1992].

Over a six-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. Vibrio parahaemolyticus infections were found in 13 patients (three with wound infections, ten with ear infections), and Vibrio alginolyticus infections in 17 patients, all of whom had ear infections. From 1987 to 1990, infections caused by marine vibrios were found in at most four cases annually, in 1992 in six instances, whereas over a five-month period in 1991 we experienced 15 cases of extraintestinal infections. Fifteen of the infections found in 1991 and 1992 were ear infections, ten of which occurred shortly after exposure to Danish coastal seawater. The disease was mild in most of the patients and all recovered. Most of the patients showed predisposing conditions such as chronic otitis media, perforation of the tympanic membrane or ulcus cruris. The organisms were isolated in minor numbers from coastal water samples from five of 12 bathing areas around Funen at the end of July, but not at the end of November. This study indicates that halophilic marine vibrios may be pathogenic in Denmark in persons exposed to seawater.

Denmark↗
PubMed2002

Cloning, characterisation and phylogenetic analysis of the fur gene in Vibrio salmonicida and Vibrio logei.

The gene encoding the ferric uptake regulator protein (fur gene) of Vibrio salmonicida 87/09/1193 was located following hybridisation of an EcoRI digest of chromosomal V. salmonicida DNA with a 316 base pairs (bp) probe internal to the fur gene of Vibrio anguillarum. A 2088 bp fragment including an open reading frame of 441 bp, encoding a protein of 147 amino acids, and homologous with fur, was identified, cloned and sequenced. A plasmid bound V. salmonicida fur gene was found capable of complementing the fur mutation of Escherichia coli H1681. Although no 'iron-box' was identified upstream of the start-codon, beta-galactosidase activity in E. coli H1681 was regulated by iron availability in the media, indicating that in V. salmonicida fur, as in other fur genes, iron functions as a co-repressor. Southern blot hybridizations demonstrated that fur is conserved amongst V. salmonicida strains and several other closely related Vibrio strains in which fur remains as yet, uncharacterized. The fur gene of Vibrio logei NCIMB 2252 was subsequently amplified using polymerase chain reaction primers external to the V. salmonicida fur gene. Comparison of phylogenetic analyses using fur and 16S DNA coding for rRNA sequences, confirmed the usefulness of fur as an evolutionary marker within the genus Vibrio.

Amino Acid Sequence↗
PubMed2003

LuxO controls luxR expression in Vibrio harveyi: evidence for a common regulatory mechanism in Vibrio.

Quorum-sensing control of luminescence in Vibrio harveyi, which involves an indirect autoinducer-mediated phosphorelay signal transduction system, contrasts with the prototypical quorum-sensing system of Vibrio fischeri, in which the autoinducer and the transcriptional activator LuxR directly activate lux operon expression. In V. harveyi, a regulator not homologous to V. fischeri LuxR and also designated LuxR (LuxRvh), binds specifically to the lux operon promoter region and activates the expression of luminescence. A direct connection has not been identified previously between V. harveyi LuxRvh and the autoinducer-mediated phosphorelay system. Here, we demonstrate by mobility shift assays and measurement of luxRvh mRNA levels with luxO+ and luxO- cells that the central response regulator of the V. harveyi phosphorelay system (LuxO) represses the level of LuxRvh. Expression of a luxRvh-bearing plasmid strongly stimulated luminescence of a luxO- mutant but had no effect on luminescence of wild-type luxO+ cells, indicating tight regulation of luxRvh by LuxO. Furthermore, luxO null mutants of V. fischeri MJ-1 and two autoinducer mutants, MJ-211 (luxI-) and MJ-215 (luxI-ainS-), emitted more light and exhibited more elevated levels of litR, a newly identified V. harveyi luxRvh homologue, than their luxO+ counterparts. These results suggest that activity of the autoinducer-mediated phosphorelay system is coupled to LuxRvh/LitR control of luminescence through LuxO in V. harveyi and V. fischeri. The presence of homologues of V. harveyi LuxRvh, LuxO and other phosphorelay system proteins in various Vibrio species and the control of LuxRvh and its homologues by LuxO identified here in V. harveyi and V. fischeri and recently in Vibrio cholerae suggest that the luxO-luxRvh couple is a central feature of this quorum-sensing system in members of the genus Vibrio.

Bacterial Proteins↗
PubMed1969

Progressive changes of Vibrio serotypes in germ-free mice infected with Vibrio cholerae.

Germ-free mice inoculated with Vibrio cholerae became colonized with vibrios throughout the gastrointestinal tract, but they did not become ill. High vibrio concentrations in the feces were observed throughout the 3 months of observation in spite of the presence of serum antibody. Reciprocal conversions of both Inaba and Ogawa serotypes occurred regularly after inoculation and could be correlated temporally with the appearance of serum-agglutinating antibody. Both of these smooth serotype were later progressively replaced by rough vibrios. Rough to smooth serotype reversions were also found in mice inoculated with a rough vibrio strain. Studies employing immunosuppression and selective vaccination suggested that such serotypic changes were a result of the selective effect of antibody within the intestinal lumen.

Agglutination↗
PubMed1966

Interaction of Vibrio cholerae and Vibrio El Tor.

Soon after its entry into the Indo-Pakistan subcontinent in 1964, cholera El Tor progressively replaced classical cholera. One of the probable reasons for this was found from laboratory studies of the interaction of the two choleragenic vibrios, V. cholerae and V. El Tor. It was observed, in both in vitro and in vivo experiments, that in mixed culture El Tor vibrios from cholera cases are capable of outgrowing and rapidly eliminating V. cholerae. The apathogenic El Tor strains from the Middle East countries differed significantly from the pathogenic type of El Tor strains isolated from cholera cases in that the former did not inhibit the growth of V. cholerae strains. The possible mechanism of interaction of the pathogenic El Tor vibrios and V. cholerae was studied in detail. Various possibilities, including higher rates of multiplication of El Tor vibrios, competition for nutrients, secretion of inhibitory substances and liberation of lethal bacteriophage or vibriocins by El Tor vibrios, were examined. Although it was not possible to establish the actual mechanism of this interaction, the possible biological effect on the epidemiology of cholera was evident.

Animals↗
PubMed1986

Report of a wound infection caused by Vibrio parahaemolyticus and Vibrio vulnificus.

The present case describes a foot wound caused by a clam shell from which both Vibrio parahaemolyticus and Vibrio vulnificus were recovered. Although extraintestinal infections associated with Vibrio parahaemolyticus have been reported previously, the simultaneous isolation of two marine vibrios from our case suggests that these organisms may coexist in mixed infections from a common source.

Adult↗
PubMed1990

Fatalities associated with Vibrio parahaemolyticus and Vibrio cholerae non-O1 infections in Florida (1981 to 1988).

Vibrio infections constitute a continuing source of morbidity and mortality in Florida. Seven fatal infections caused by Vibrio parahaemolyticus or V cholerae non-O1 were reported in Florida between 1981 and 1988. Review of those seven medical records and Vibrio case investigation forms showed that although all patients died of sepsis, gastrointestinal signs and symptoms characterized the early illness in four patients, whereas the other three initially had painful swelling and/or lesions of the lower extremities. All patients had preexisting chronic diseases. Five patients (71%) had eaten seafood during the week before oneset of illness, including four (57%) who had eaten raw oysters. To reduce the risk of acquiring Vibrio infections, raw or undercooked seafood should be eliminated from the diet, particularly by persons with underlying chronic diseases.

Aged↗
PubMed1982

Vibrio parahaemolyticus and related halophilic Vibrios.

Approximately 30 years have elapsed since Dr. Fujino's original discovery that Vibrio parahaemolyticus (then termed Pasteurella parahemolytica) was the cause of "summer diarrhea" in Japan. Since that finding, V. parahaemolyticus has been established as a cause of gastroenteritis in numbers and places approaching global proportions. It has been isolated in marine and estuarine areas almost worldwide and despite its halophilic nature, V. parahaemolyticus has been isolated from saline-free waters. The relationship of this organism to the environment reveals a close association with other marine organisms especially copepods on which the Vibrios depend for survival in winter months and growth in summer months. There is a uniquely provocative disparity between human strains of V. parahaemolyticus which are Kanagawa phenomenon (KP) positive and the environmental strains which to a large extent are KP negative, the significance being that pathogenicity is measured according to the Kanagawa phenomenon (hemolytic activity) reaction. The hemolysin of the pathogenic strains is a thermostable, cardiotoxic protein, which thus far has not been implicated in the mechanism(s) which causes human gastroenteritis. The interest in this organism has been widened in recent years by the finding that similar organisms, V. alginolyticus, lactose positive vibrios and group F vibrios also cause serious disease in humans.

Animals↗
PubMed2026

Vibrio phycocola sp. nov. and Vibrio phycohabitans sp. nov., Isolated from the Phycosphere of Marine Algae.

Two Gram-stain-negative, facultatively aerobic, oxidase- and catalase-positive, motile (by means of a polar flagellum) rod-shaped bacterial strains, designated BS-M-Sm-2T and MA40-2T, were isolated from marine algae. Growth was optimal at pH 7.0-8.0 and 2.0-3.0% (w/v) NaCl, with temperature optima of 25°C for BS-M-Sm-2T and 25-30°C for MA40-2T. Ubiquinone-8 was the sole respiratory quinone. The major fatty acids common to both strains were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), while BS-M-Sm-2T additionally contained C12:0 and C14:0. The predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol, with diphosphatidylglycerol also detected in strain MA40-2T. The DNA G+C contents of strains BS-M-Sm-2T and MA40-2T were 44.2 and 39.8 mol%, respectively. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) values between the two strains were 93.8%, 71.4%, and 23.2%, respectively. Phylogenetic and phylogenomic analyses placed both strains within the genus Vibrio, forming distinct lineages. Comparisons with closely related Vibrio type strains yielded ANI and dDDH values below 91.6% and 44.3%, respectively, further supporting their classification as novel species. Genome analyses revealed genes potentially involved in algal symbiosis, including those for polysaccharide degradation and vitamin biosynthesis. Based on comprehensive genomic, phylogenetic, phenotypic, and chemotaxonomic evidence, strains BS-M-Sm-2T and MA40-2T represent two novel species, for which the names Vibrio phycocola sp. nov. (BS-M-Sm-2T =KACC 24066T =DSM 119941T) and Vibrio phycohabitans sp. nov. (MA40-2T =KACC 24064T = DSM 119942T) are proposed.

RNA, Ribosomal, 16S↗
PubMed2005

Vibrio parahaemolyticus, Vibrio vulnificus and microorganisms of fecal origin in mussels (Mytilus galloprovincialis) sold in the Puglia region (Italy).

Mytilus galloprovincialis is one of the most commonly consumed of all bivalve molluscs. The consumption of raw bivalve molluscs has caused outbreaks of food poisoning due to Vibrio parahaemolyticus and Vibrio vulnificus. This paper reports the results of a survey on the presence of V. parahaemolyticus, V. vulnificus fecal coliform bacteria, Escherichia coli and Salmonella spp. in 600 M. galloprovincialis samples collected from retail outlets in the Puglia region. V. parahaemolyticus and V. vulnificus were found in 47 (7.83%) and 17 (2.83%) of the samples, respectively. One sample (0.16%) was contaminated with Salmonella spp. but no relationship was observed between vibrios and fecal coliforms and E. coli. There were no significant differences among vibrios present in bivalve molluscs during the 3-year survey.

Animals↗
PubMed1988

O-antigenic lipopolysaccharides isolated from a marine Vibrio, bio-serogroup 1875, possessing an antigenic factor in common with O1 Vibrio cholerae.

The chemical and serological characteristics of lipopolysaccharides (LPS) isolated from Vibrio bio-serogroup 1875 were compared with those of O1 Vibrio cholerae LPS. Vibrio bio-serogroup 1875 LPS contained all the component sugars which were found in O1 V. cholerae LPS, i.e. glucose, L-glycero-D-manno-heptose, fructose, glucosamine, perosamine and quinovosamine, though the amount of perosamine, a characteristic component of O1 V. cholerae LPS, was very low compared with that of O1 V. cholerae LPS. Their LPS additionally contained mannose and two unidentified neutral sugars which are not regular constituents of O1 V. cholerae LPS. Definite serological cross-reactivity in the passive haemolysis test between LPS from Vibrio bio-serogroup 1875 and LPS from O1 V. cholerae was demonstrated.

Antigens, Bacterial↗
PubMed2001

Analysis of seawaters for the recovery of culturable Vibrio parahaemolyticus and some other vibrios.

We investigated the recovery of dormant and injured cells along with the normally culturable cells of Vibrio species with special emphasis on V. parahaemolyticus using both selective and non-selective media at moderate (20 C) and standard (37 C) culture temperatures from a bay water environment. Culture temperatures (20 or 37 C) did not affect the recovery of V. parahaemolyticus but did for other vibrios. We observed similar seasonality of V parahaemolyticus as in most other environmental studies. V. parahaemolyticus and other Vibrio species were recovered in higher numbers by a replica plating method compared to most probable number (MPN) and direct TCBS (thiosulfate citrate bile-salt sucrose) agar counts. Even with the replica plating method, however, vibrios number goes down to a minimum level and V. parahaemolyticus was undetectable during the cool temperature period of the year, although total bacterial cells and CFU on nutrient agar (with 2% NaCl) did not vary so much during the study period.

Colony Count, Microbial↗
PubMed1987

A bioserogroup of marine vibrios possessing somatic antigen factors in common with Vibrio cholerae O1.

A bioserogroup of halophilic vibrios, tentatively labelled bioserogroup 1875, strongly agglutinated by O1 antiserum of Vibrio cholerae is described. Cross-agglutination and agglutinin-absorption tests showed that these vibrios had antigens identical with the Ogawa and Inaba factors of V. cholerae O1, although they possessed their own specific antigen distinctive from the A factor that is the specific major antigen of the latter species. In addition, quantitative antigen variation similar to that of the Ogawa-to-Inaba type with V. cholerae O1 was recognized in this halophilic group. They were isolated from estuarine water and are considered to inhabit coastal aquatic environments. Phenotypically, however, this group is not identifiable with any of the species already recognized in the genus Vibrio.

Agglutination Tests↗
PubMed2006

Inhibition of Vibrio parahaemolyticus by a bacteriocin-like inhibitory substance (BLIS) produced by Vibrio mediterranei 1.

AIMS: The aim of this research was to identify and partially purify new bacteriocin-like substances from strains of halophilic 'non-cholera' vibrios isolated from food sources. METHODS AND RESULTS: Forty-five halophilic Vibrio spp. strains were screened for antimicrobial production. Vibrio mediterranei 1, a nonpathogenic strain, showed antimicrobial activity towards Vibrio parahaemolyticus spp. and related species. The bacteriocin-like inhibitory substance (BLIS), released by the bacteria into growth media, was concentrated by ultrafiltration and characterized. BLIS was sensitive to proteinase K, was stable in the pH range 5-9, was resistant to organic solvents and was heat stable up to 75 degrees C. Initial purification of BLIS by size exclusion chromatography showed an apparent molecular mass of 63-65 kDa. CONCLUSIONS: This study reports the ability of V. mediterranei 1 to produce a bacteriocin-like substance inhibiting growth of V. parahaemolyticus spp. and other closely related bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: The strong activity of BLIS towards the human and fish pathogen V. parahaemolyticus and the persistence of antimicrobial properties under a variety of different conditions suggest its potential application in food microbiology.

Antibiosis↗
PubMed1985

Detection of the thermostable direct hemolysin gene and related DNA sequences in Vibrio parahaemolyticus and other vibrio species by the DNA colony hybridization test.

A specific gene probe for the Vibrio parahaemolyticus thermostable direct hemolysin gene was constructed and used to examine the presence or absence of the thermostable direct hemolysin gene or related DNA sequences in V. parahaemolyticus and other vibrios by the DNA colony hybridization method. The gene probe consisted of a 406-base-pair, completely internal fragment covering 71% of the structural gene with PstI linkers added to the ends. Six copies of this 415-base-pair PstI fragment were cloned into plasmid pBR322, which yielded large amounts of the probe DNA. One hundred forty-one V. parahaemolyticus strains were tested with the gene probe, and the results were compared with those of phenotypic assays for the thermostable direct hemolysin. All Kanagawa phenomenon-positive strains were gene positive. However, 86% of the strains that exhibited weak Kanagawa phenomenon and 16% of Kanagawa phenomenon-negative strains also reacted with the gene probe. Immunological methods for the detection of the thermostable direct hemolysin (modified Elek test, enzyme-linked immunosorbent assay) showed better correlation with gene probe results. All gene-positive strains produced hemolysin detectable in the enzyme-linked immunosorbent assay, although occasional strains showed weak reaction. The modified Elek test was slightly less sensitive than the enzyme-linked immunosorbent assay. All gene-negative strains were also negative in these immunological assays. One hundred twenty-one strains of Vibrio spp. other than V. parahaemolyticus were tested with the gene probe; only Vibrio hollisae strains reacted with the probe under stringent conditions.

Base Sequence↗
PubMed2000

The virulence regulatory protein ToxR mediates enhanced bile resistance in Vibrio cholerae and other pathogenic Vibrio species.

The transmembrane regulatory protein ToxR is required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, including cholera toxin (CT) and the toxin coregulated pilus (TCP). ToxR is necessary for transcription of the gene encoding a second regulatory protein, ToxT, which is the direct transcriptional activator of CT and TCP genes. However, ToxR, independent of ToxT, directly activates and represses transcription of the outer membrane porins OmpU and OmpT, respectively. The genes encoding TCP and CT (and including ToxT) lie on horizontally acquired genetic elements, while the toxR, ompU, and ompT genes are apparently in the ancestral Vibrio chromosome. The contribution of ToxR-dependent modulation of outer membrane porins to cholera pathogenesis has remained unknown. We demonstrate that ToxR mediates enhanced bile resistance in a ToxT-independent manner. In both classical and El Tor biotypes of V. cholerae, a toxR mutant strain has a reduced minimum bactericidal concentration (MBC) of bile, the bile component deoxycholate (DC), and the anionic detergent sodium dodecyl sulfate (SDS) compared to both wild-type and toxT mutant strains. Classical and El Tor toxR mutant strains also exhibit reduced growth rates at subinhibitory concentrations of DC and SDS. Growth of either V. cholerae biotype in subinhibitory concentrations of bile or DC induces increased ToxR-dependent production of a major 38-kDa outer membrane protein, which was confirmed to be OmpU by Western blot. Measurement of transcription of a ompUp-lacZ fusion in both biotypes reveals stimulation (about two- to threefold) of ToxR-dependent ompU transcription by the presence of bile or DC, suggesting that ToxR may respond to the presence of bile. The toxR mutant strains of three additional human intestinal pathogenic Vibrio species, V. mimicus, V. fluvialis, and V. parahaemolyticus, display lower MBCs of bile, DC, and SDS and have altered outer membrane protein profiles compared to the parental wild-type strains. Our results demonstrate a conserved role for ToxR in the modulation of outer membrane proteins and bile resistance of pathogenic Vibrio species and suggest that these ToxR-dependent outer membrane proteins may mediate enhanced resistance to bile. We speculate that ToxR-mediated bile resistance was an early step in the evolution of V. cholerae as an intestinal pathogen.

Adhesins, Bacterial↗