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PubMedDate not supplied

Cytokine production and protein adsorption in a stainless steel filter used for leukocyte reduction.

A new metallic filter made from a stainless steel fiber has been under development. To evaluate biocompatibility of this filter, the authors compared cytokine production with that of stainless steel fibers and polyester fibers by using a mononuclear cell culture. Furthermore, adsorbed proteins on each fiber were identified by using sodium-dodecyl sulfate (SDS)-polyacrlyamide gel electrophoresis (PAGE). The levels of tumor necrosis factor (TNF)-alpha in the cultured supernatant without fibers as the control, with polyester fibers, and with stainless steel fibers were 28.1 +/- 8.1, 39.3 +/- 2.6, and 29.1 +/- 6.7 pg/ml, respectively. The levels of interleukin (IL)-1 beta were 7.6 +/- 3.2, 8.9 +/- 1.5, and 8.9 +/- 2.1 pg/ml, respectively. The IL-4 levels were less than 0.25 pg/ml, and the interferon-tau levels were less than 7.8 pg/ml in all three conditions. The amount of adsorbed proteins was 3.39 +/- 0.27 microgram/cm2 for the polyester fibers and 2.72 +/- 0.23 microgram/ cm2 for the stainless steel fibers. The protein bands adsorbed to the polyester fibers by SDS-PAGE analysis were observed at approximately 180, 120, 90, 76, 67, 59, 56, and 28 kd molecular weight. In contrast, the protein bands adsorbed to the stainless steel fibers were observed at 90, 76, 67, 62, 56, 28, and 12 kd molecular weight. Thus, the proteins adsorbed to the stainless steel fibers differed from those on the polyester fibers. By western blot analysis, the amounts of albumin, IgG tau chain, and fibronectin adsorbed on the stainless steel fibers were smaller than those on the polyester fibers. The results of this study suggest that the stainless steel fibers do not stimulate monocytes, Th1, and Th2 cells. In addition, lesser adsorption of IgG tau chain and fibronectin may indicate that the stainless steel is a superior material for anti thrombogenicity compared to polyester.

Adsorption↗

PubMed2002

Highly robust stainless steel tips as microelectrospray emitters.

Tapered stainless steel spray tips for sheathless microelectrospray ionization (microESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the microESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the microESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications.

Chromatography, Liquid↗

PubMed1999

Heat transfer analysis of staphylococcus aureus on stainless steel with microwave radiation.

Staphylococcus aureus (NCTC 6571; Oxford strain) on stainless steel discs was exposed to microwave radiation at 2450 MHz and up to 800 W. Cell viability was reduced as the exposure time increased, with complete bacterial inactivation at 110 s, attaining a temperature of 61.4 degrees C. The low rate of temperature rise, RT, of the bacterial suspension as compared with sterile distilled water or nutrient broth suggests a significant influence of the microwave sterilization efficacy on the thermal properties of the micro-organisms. The heat transfer kinetics of thermal microwave irradiation suggest that the micro-organism has a power density at least 51-fold more than its surrounding liquid suspension. When the inoculum on the stainless steel disc was subjected to microwave radiation, heat conduction from the stainless steel to the inoculum was the cause of bacteriostasis with power absorbed at 23.8 W for stainless steel and 0.16 W for the bacteria-liquid medium. This report shows that the microwave killing pattern of Staph. aureus on stainless steel was mainly due to heat transfer from the stainless steel substrate and very little direct energy was absorbed from the microwaves.

Hot Temperature↗

PubMed2003

Pitting inhibition of stainless steel by surfactants: an electrochemical and surface chemical approach.

Pitting corrosion of stainless steels causes tremendous damage in terms of material loss and resulting accidents. Organic surfactants have been tried as pitting inhibitors but the understanding of the inhibition mechanisms is mainly speculative. In the present study the inhibition of the pitting corrosion of 304 stainless steel by N-lauroylsarcosine sodium salt (NLS) in 0.1 M NaCl solutions at neutral pH was studied using an approach that combines surface chemical techniques with electrochemical ones. It was found that NLS increases the pitting resistance of 304 stainless steel, with possible complete inhibition at high NLS concentration (30 mM). Adsorption of NLS on 304 stainless steel particles was directly measured. NLS adsorbs significantly on 304 stainless steel with maximum adsorption density close to bilayer coverage. Electrophoretic mobility data for 304 stainless steel particles show that the surface of 304 stainless steel is negative in NaCl solution at neutral pH. The adsorption of NLS makes the interfacial charge even more negative. The relationship between pitting inhibition and adsorption density of NLS suggests that NLS does not adsorb preferentially on the pit nucleation sites and complete inhibition requires that the whole surface be covered completely by NLS. The inhibition mechanism of NLS is proposed to be due mainly to the blocking effect of a negatively charged NLS adsorption layer. This study shows that in addition to the adsorption amount of surfactant, interfacial charge also plays an important role in pitting inhibition.

Journal Article↗

PubMed1980

Automated detection of microbial growth in blood cultures by using stainless-steel electrodes.

Sterile stainless-steel electrodes implanted in blood culture bottles and monitored electronically were used to detect growth of microorganisms. Each blood culture bottle contained 100 ml of medium and was inoculated with 10 ml of blood seeded with either 300 or 50 colony-forming units of one of several bacterial or yeast species that are commonly isolated from clinical blood cultures. Growth was indicated by a voltage change of at least 0.1 mV/min with an increasing slope over at least three consecutive 15-min intervals. This method was compared to the conventional visual method for detecting microbial growth in broth. Growth detection by both techniques was confirmed by subculture to solid media. Of the 163 cultures seeded with the high inoculum (300 colony-forming units) and confirmed as being positive, 148 (90.8%) were positive by the electronic detection system (EDS). At the lower inoculum (50 colony-forming units), 47 of 53 (88.7%) positive cultures were detected by EDS. Twelve of the 21 false-negatives by the EDS were cultures seeded with Cryptococcus neoformans. Excluding C. neoformans, the rate of detection of growth was 96.0%. Microbial growth was detected an average of 18 h earlier by EDS than by the conventional system in 176 (90.2%) of the cultures. Also examined were 156 patient blood cultures: 13 were positive both by EDS and by conventional methods.

Bacteria↗

PubMed1994

Toxic effects caused by stainless steel corrosion products on mouse seminiferous cells.

Stainless steel type AISI 316L is widely used in orthopaedic surgery. The toxic effects of its corrosion products on the morphology of mouse seminiferous cells were investigated. Chemical elements from stainless steel were released into a physiological medium using an electrochemical method. This metallic solution was injected subcutaneously into male Charles River mice at 72 h intervals for 10 days. Electron microscopic observations of seminiferous tubule thin sections showed that the metallic suspension caused tissue vacuolation, cell degeneration, and multinucleated cell formation. This apparent tissue toxicity induced by stainless steel corrosion products suggests that long term implantation of such biomaterials may impair spermatogenesis.

Animals↗

PubMed1999

Preformed posterior stainless steel crowns: an update.

For almost 50 years, dentists have used stainless steel crowns for primary and permanent posterior teeth. No other type of restoration offers the convenience, low cost, durability, and reliability of such crowns when interim full-coronal coverage is required. Preformed stainless steel crowns have improved over the years. Better luting cements have been developed and different methods of crown manipulation have evolved. This article reviews stainless steel crown procedures for primary and permanent posterior teeth. Step-by-step placement of a primary molar stainless steel crown is documented and permanent molar stainless steel crown restoration is described. A method for repairing a worn-through crown also is reviewed.

Bicuspid↗

PubMed1997

Nickel release from stainless steels.

In 1994, a study of nickel release and allergic contact dermatitis from nickel-plated metals and stainless steels was published in this journal. It was shown that low-sulfur stainless steel grades like AISI 304, 316L or 430 (S < or = 0.007%) release less than 0.03 microgram/cm2/week of nickel in acid artificial sweat and elicit no reactions in patients already sensitized to nickel. In contrast, nickel-plated samples release around 100 micrograms/cm2/week of Ni and high-sulfur stainless steel (AISI 303-S approximately 0.3%) releases about 1.5 micrograms/cm2/week in this acid artificial sweat. Applied on patients sensitized to nickel, these metals elicit positive reactions in 96% and 14%, respectively, of the patients. The main conclusion was that low-sulfur stainless steels like AISI 304, 316L or 430, even when containing Ni, should not elicit nickel contact dermatitis, while metals having a mean corrosion resistance like a high-sulfur stainless steel (AISI 303) or nickel-plated steel should be avoided. The determining characteristic was in fact the corrosion resistance in chloride media, which, for stainless steels, is connected, among other factors, to the sulfur content. Thus, a question remained concerning the grades with an intermediate sulfur content, around 0.03%, which were not studied. They are the object of the study presented in this paper. 3 tests were performed: leaching experiments, dimethylglyoxime and HNO3 spot tests, and clinical patch tests; however, only stainless steels were tested: a low-sulfur AISI 304 and AISI 303 as references and 3 grades with a sulfur content around 0.03%: AISI 304L, AISI 304L added with Ca, AISI 304L+Cu. Leaching experiments showed that the 4 non-resulfurised grades released less than 0.5 microgram/cm2/week in acid sweat while the reulfurized AISI 303 released around or more than 0.5 microgram/cm2/week. This is explained by the poorer corrosion resistance of the resulfurized grade. Yet all these grades had the same reaction to the DMG test (negative result), which shows again its lack of sensitivity. In contrast, the HNO3 spot test distinguished AISI 303 from the non-resulfurized grades. Clinical patch tests again showed that some patients (4%) were intolerant to AISI 303, while none were intolerant to the other grades. Thus, this study confirms that non-resulfurized stainless steels (S < or = 0.03%) like Ni-containing 304 and 304L should not elicit Ni contact dermatitis, while the resulfurized grades (S > 0.1%) should be avoided.

Adolescent↗

PubMed2007

Attachment of Shiga toxigenic Escherichia coli to stainless steel.

Shiga toxigenic Escherichia coli (STEC) are important foodborne pathogens causing gastrointestinal disease worldwide. Bacterial attachment to food surfaces, such as stainless steel may lead to cross contamination of foods and subsequent foodborne disease. A variety of STEC isolates, including E. coli O157:H7/H- strains, were grown in planktonic (broth) and sessile (agar) culture, following which initial attachment to stainless steel was determined using epifluorescence microscopy. Experiments were performed to determine whether the number of bacteria attached to stainless steel differed between STEC strains and between the two modes of growth. No relationship was found between STEC strains and the number of bacteria attached to stainless steel. Five STEC strains, including one non-toxigenic O157 isolate, attached in significantly greater (p<0.05) numbers to stainless steel following growth in planktonic culture compared to sessile culture. In contrast, two clinical strains of O157:H7 attached in significantly greater (p<0.05) numbers following growth in sessile culture compared to planktonic culture. Thirteen out of twenty E. coli strains showed no significant difference (p>0.05) in attachment when grown in planktonic or sessile culture. The change of interfacial free energy between the bacterial strains and stainless steel was calculated and the influence of free energy in attachment was determined. Although a significant variation (p<0.05) in free energy values was found between STEC strains, no correlation was found between free energy values and bacterial counts on stainless steel. In addition, no correlation was also found between bacterial hydrophobicity and surface charge values or production of surface structures (type I fimbriae or flagella) (previously determined) with the number of bacteria attached to stainless steel. The results of this study suggest that different growth conditions (planktonic and sessile) can influence the attachment of STEC to stainless steel. Factors other than bacterial physicochemical properties and these surface structures may also influence STEC attachment to stainless steel.

Bacterial Adhesion↗

PubMed1983

Metal determination in organic fluids of patients with stainless steel hip arthroplasty.

In 20 stainless steel Charnley hip arthroplasties (with a follow-up of 10-13 years) nickel, chromium and manganese levels were measured in blood, plasma and urine by atomic absorption spectrophotometry. Skin patch tests for these metals, and clinical and roentgenographic results of arthroplasty were also assessed. Metal levels in organic fluids were plotted against a control population homogeneous for age, residence and anamnestic conditions with the first, but which had never undergone a prosthesis or other metallic implant surgical procedure. Nickel levels in blood, plasma and urine, manganese levels in blood and urine and chromium levels in plasma were significantly higher in the hip prostheses population. Metal ion release from stainless steel prostheses is discussed with regard to implant failure, metal sensitivity and carcinogenesis.

Aged↗

PubMed1998

Multicycle mechanical performance of titanium and stainless steel transpedicular spine implants.

STUDY DESIGN: This was a prospective in vitro study comparing titanium alloy and stainless steel alloy in transpedicular spine implants from two different manufactures. OBJECTIVE: To compare the multicycle mechanical performance of these two alloys, used in each of two different implant designs. SUMMARY OF BACKGROUND DATA: Transpedicular spine implants primarily have been manufactured from stainless steel, but titanium alloy offers imaging advantages. However, the notch sensitivity of titanium alloy has caused concern regarding how implants made from this material will compare in stiffness and fatigue life with implants made from stainless steel. METHODS: Twenty-four implants (two alloys, two designs, six implants per group) were mounted in machined polyethylene wafers and repetitively loaded (up to 1 million cycles) from 80 N to 800 N using a 5-Hertz sinusoidal waveform. Load and displacement data were automatically and periodically sampled throughout the entire test. RESULTS: Implant stiffness increased with cycle load number, reached a steady state, then declined just before fatigue failure. Stiffness varied less in titanium transpedicular spine implants than in their stainless counterparts. All stainless steel implant types were stiffer (steady-state value, P < 0.0001) than their titanium alloy counterparts. One titanium implant design failed with fewer (P < 0.05) load cycles than its stainless steel counterpart, whereas a stainless steel implant of another design failed with fewer (P < 0.002) load cycles than its titanium counterpart. Overall, fatigue life, i.e., the total number of load cycles until failure, was related to implant type (P < 0.0001), but not to implant material. CONCLUSIONS: A transpedicular spine implant's fatigue lifetime depends on both the design and the material and cannot be judged on material alone. Stainless steel implants are stiffer than titanium alloy implants of equal design and size; however, for those designs in which the fatigue life of the titanium alloy version is superior, enlargement of the implant's components can compensate for titanium's lower modulus of elasticity and result in an implant equally stiff as its stainless steel counterpart. Such an implant made from titanium alloy would then be clinically preferable because of titanium's previously reported imaging advantages.

Elasticity↗

PubMed1998

Corrosion behavior of sensitized duplex stainless steel.

The present work investigates the corrosion behavior of 2205 duplex stainless steel in 0.9% NaCl solution after various heat-treatments, and compares it to that of 316L austenitic stainless steel. Both stainless steels were heat-treated at 500, 650, and 800 degrees C in air for 1 h, followed by furnace cooling. Each heat-treated sample was examined for their microstructures and Vickers micro-hardness, and subjected to the X-ray diffraction for the phase identification. Using potentiostatic polarization method, each heat-treated sample was corrosion-tested in 37 degrees C 0.9% NaCl solution to estimate its corrosion rate. It was found that simulated sensitization showed an adverse influence on both steels, indicating that corrosion rates increased by increasing the sensitization temperatures.

Corrosion↗

PubMed1992

The effects of cold treatment on the physical properties of stainless steel and titanium alloy endodontic posts and instruments.

This study determined whether cold treatment at -96 degrees C affected the mechanical properties of different compositions and sizes of Flexi-Post systems, various sizes of dental reamers, and external wrenches. The experiment was divided into eight groups. The four control groups were untreated and consisted of (1) 50 stainless steel posts, (2) 40 titanium posts, (3) 50 stainless steel reamers, and (4) 10 stainless steel external wrenches. The four experimental groups were cold treated once and twice and included (1) 100 stainless steel posts, (2) 80 titanium posts, (3) 100 stainless steel reamers, and (4) 20 stainless steel external wrenches. The bending or breaking forces were tested on a universal testing machine. Stainless steel Flexi-Post dowels, stainless steel reamers, and external wrenches all showed a significant (ANOVA) (p < 0.05) increase in strength as a result of the single and double cold treatment. Titanium posts showed no strength increase following a single cold treatment, while they were weakened by a double cold treatment.

Analysis of Variance↗

PubMed1990

Altered corrosion resistance from casting to stainless steel posts.

Heat treatment during the burnout procedure may cause corrosion of the stainless steel post. This study was undertaken to determine whether alteration of the corrosion resistance of stainless steel posts occurs as a result of various simulated burnout procedures. Stainless steel posts were divided into five groups of five posts: group 1, control; 2, gypsum-bonded investment, bench cooled; 3, gypsum-bonded investment, quenched; 4, phosphate-bonded investment, bench cooled; and 5, phosphate-bonded investment, quenched. The posts were placed in individual containers of Ringer's solution and observed at 30, 180, and 600 days. Scanning electron microscopy, energy dispersive x-ray spectroscopy, optical emission spectroscopy, and optical microscopy were used to evaluate the posts qualitatively and quantitatively. Investment and heat treatment altered the metallic structure of stainless steel posts. Prefabricated posts submitted to simulated burnout procedures had a noticeable reduction in corrosion resistance. Direct casting to stainless steel posts is contraindicated.

Corrosion↗

PubMed1995

The particle size distribution, density, and specific surface area of welding fumes from SMAW and GMAW mild and stainless steel consumables.

Particle size distributions were measured for fumes from mild steel (MS) and stainless steel (SS); shielded metal arc welding (SMAW) and gas metal arc welding (GMAW) consumables. Up to six samples of each type of fume were collected in a test chamber using a micro-orifice uniform deposit (cascade) impactor. Bulk samples were collected for bulk fume density and specific surface area analysis. Additional impactor samples were collected using polycarbonate substrates and analyzed for elemental content. The parameters of the underlying mass distributions were estimated using a nonlinear least squares analysis method that fits a smooth curve to the mass fraction distribution histograms of all samples for each type of fume. The mass distributions for all four consumables were unimodal and well described by a lognormal distribution; with the exception of the GMAW-MS and GMAW-SS comparison, they were statistically different. The estimated mass distribution geometric means for the SMAW-MS and SMAW-SS consumables were 0.59 and 0.46 micron aerodynamic equivalent diameter (AED), respectively, and 0.25 micron AED for both the GMAW-MS and GMAW-SS consumables. The bulk fume densities and specific surface areas were similar for the SMAW-MS and SMAW-SS consumables and for the GMAW-MS and GMAW-SS consumables, but differed between SMAW and GMAW. The distribution of metals was similar to the mass distributions. Particle size distributions and physical properties of the fumes were considerably different when categorized by welding method. Within each welding method there was little difference between MS and SS fumes.

Aerosols↗

PubMedDate not supplied

Corrosive effect of carbon-fibre reinforced plastic on stainless-steel screws during implantation into man.

The corrosion of stainless-steel screws used to fix carbon-fibre reinforced plastic (CFRP) plates to human fractures was compared with the corrosion on similar screws used to fix stainless-steel AO plates. Corrosive changes were noted in both sets of screws with similar frequency and severity; however, the stainless-steel plates were 'in situ' almost twice as long as the CFRP ones, showing that the corrosive changes occurred more rapidly on screws in contact with CFRP. Nevertheless, over the implantation time necessary for bone healing, corrosion was very mild and there is no clinical contra-indication to the use of stainless-steel and CFRP together in this particular application.

Bone Screws↗

PubMed1997

Biomechanical assessment of titanium and stainless steel posterior spinal constructs: effects of absolute/relative loading and frequency on fatigue life and determination of failure modes.

The goal of this study was to examine the effects of absolute/relative loads and frequency on the fatigue life of titanium and stainless steel posterior spinal constructs, and to determine the failure fracture modes. The stainless steel constructs had higher stiffness and yield strength than the titanium constructs, but the ultimate static strength was almost equal for both types of constructs. Titanium constructs, however, exhibited higher variability than the stainless steel constructs. In fatigue tests, the stainless steel constructs were significantly affected by the external load and were frequency independent. It appears from fatigue curves that 500 N can be approximated as the endurance limit for the stainless steel constructs. Titanium constructs were load-frequency dependent, and their endurance limit was somewhere between the 500 and 750 N load levels. There were no differences in performance between the stainless steel and titanium constructs at 16 Hz. At 4 Hz, titanium constructs performed as well or better than stainless steel constructs. Also, the titanium constructs resulted in better performance than the stainless steel constructs in the elastic region, and with smaller differences in the plastic region. Most of the failure modes for stainless steel constructs were in screw bending at 16 Hz with a smaller percentage of rod fractures at high loads, with a higher percentage of rod fractures observed for the stainless steel constructs at 4 Hz. Most of the failure modes for titanium constructs occurred in screw bending or fracture.

Biomechanical Phenomena↗

PubMed1979

In vivo evaluation of a high-strength, high-ductility stainless steel for use in surgical implants.

A high-strength, high-ductility, austenitic stainless steel has been evaluated for use in surgical implants by performing in vivo tests in rats, rabbits, dogs, and rhesus monkeys. This stainless steel, a TRIP (Transformation Induced Plasticity) steel containing about 4% Mo, was compared with two alloys in current clinical use: Type 316L stainless steel and cast Vitallium. Compared with the other two alloys, cast Vitallium generally had higher resistance to corrosion and superior biocompatibility in all animals. The tests in rats and dogs indicated that the corrosion resistances of the TRIP steel and the Type 316L stainless steel were similar and that the tissue reactions caused by these alloys were also similar. However, in rhesus monkeys, the TRIP steel was shown to be susceptible to stress-corrosion cracking and much more susceptible to crevice corrosion than Type 316L stainless steel. Limited tests in rabbits supported the observation that the TRIP steel is susceptible to stress-corrosion cracking. These inconsistencies in the in vivo tests underline the need for a reevaluation of the popular test techniques and of the animals commonly chosen for assessing the suitability of candidate implant materials. The "worst case" results from the rhesus monkey tests were entirely consistent with previous results obtained from in vitro studies. However, further work must be performed before the behavior of metals in humans, rhesus monkeys, or any other animal, can be predicted with confidence from an in vitro test program.

Animals↗