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At least 73 records · Page 4Linked to original sources
PubMed2005

Immunostimulatory effects of a polysaccharide-rich substance with antitumor activity isolated from black currant (Ribes nigrum L.).

The fruit juice of black currant was found to contain a polysaccharide-rich substance, which was designated cassis polysaccharide (CAPS), with macrophage-stimulating activity. Especially, its interleukin (IL)-1beta-inducing activity was remarkably high, compared with other fruit juice preparations. CAPS was found to consist of rhamnose, mannose, arabinose, galactose, xylose, and glucose in a molar ratio of 11.3:0.9:54.1:29.8:2.0:1.9. CAPS turned out to be partitioned into a soluble component (CAPS-l.m.) and a precipitable component (CAPS-h.m.) with mean MWs of 80,000 and 600,000 respectively in 45% (v/v) ethanol solution. At least in vitro, CAPS-l.m. rather than CAPS-h.m. appeared to play an important role in macrophage activation. Oral administration of black currant juice and CAPS to Ehrlich carcinoma-bearing mice retarded the growth of the solid tumor by 45% and 51% respectively. CAPS administration had a stimulatory effect on the release of IL-2, IL-10, interferon-gamma, and IL-4 from splenocytes in comparison with PBS treatment in tumor-bearing mice. The IL-4 level was, however, still lower than that exhibited by a group of normal mice. CAPS showed a certain cytotoxicity directly against tumor cells.

Animals↗
PubMed2002

Mixed infection of black currant (Ribes nigrum L.) plants with Blackcurrant reversion associated virus and rhabdovirus-like particles with symptoms of black currant reversion disease.

Black currant plants of cvs. Black Smith and Karlstejnský dlouhohrozen showing symptoms of severe Russian (R) form of black currant reversion disease (BCRD) were found in 1999-2000 in the Czech Republic. Five selected plants of both cultivars originating from two distant loci were tested by polymerase chain reaction (PCR) for presence of the Blackcurrant reversion associated virus (BRAV), the causal agent of BCRD. In all plants, virus-specific 215 nt cDNA fragments proving the presence of BRAV were obtained. Moreover, in two of those five black currant plants, rhabdovirus-like particles were found in ultrathin sections by electron microscopical examinations. The particles measured 200-347 nm by 64-90 nm. They occurred mostly within nuclei of parenchyma cells of vascular bundles as single particles, rafts of particles, but also in aggregates. They were found also in the perinuclear space and occasionally directly in the cytoplasm. Clusters of particles either within the nucleus or in the perinuclear space were membrane-bound. We bring evidence on the occurrence of the severe (R) form of BCRD and the first evidence of BRAV in the Czech Republic.

Electrophoresis↗
PubMed1997

Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit.

Extraction of high-quality RNA from blackcurrant fruit has hitherto proved difficult, probably owing to high levels of phenolic and polysaccharide components in the berries. The procedure described here is a modification of one described for grape berries, and yields RNA suitable for in vitro translations, RNA blot analysis, and cDNA library construction.

Fruit↗
PubMed1998

The isolation of genomic DNA from blackcurrant (Ribes nigrum L.).

A method is described for isolating DNA of high molecular mass (M(r)) from blackcurrant and other softfruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.

DNA, Plant↗
PubMed2001

Preparative-scale isolation of four anthocyanin components of black currant (Ribes nigrum L.) fruits.

Four anthocyanin components of black currant, delphinidin 3-O-beta-rutinoside (D3R), cyanidin 3-O-beta-rutinoside (C3R), delphinidin 3-O-beta-glucoside (D3G), and cyanidin 3-O-beta-glucoside (C3G), were successfully isolated as crystalline forms on a preparative scale. In this process, selective hydrolysis of the glucosides (D3G and C3G) and rhamnosides (D3R and C3R) was achieved by treatment with beta-glucosidase and hesperidinase (alpha-rhamnosidase), respectively, to improve resolution of anthocyanin components. Especially, selective conversion of the rutinosides into glucosides made the amounts of D3G and C3G increase about 4- and 7-fold, respectively. D3R, C3R, D3G, and C3G were isolated from enzymatic hydrolysates of black currant anthocyanins through Amberlite XAD-7HP absorption followed by preparative HPLC separation, and their crystals were obtained as the flavylium chloride.

Anthocyanins↗
PubMed1992

Utilization of hearing aids issued by the public health service. Hearing aid use in Ribe County, Denmark.

A prospective survey was carried out to evaluate the efficiency of public audiological rehabilitation. Two hundred and fifty-four of 256 consecutive patients were interviewed six months after hearing aid issue. One hundred and sixty-eight (66%) were full-time users, 225 (88%) wore an aid either all the time or every day. Eight were successful situational users, while 21 (8%) were non-users. Eighty-six of the 233 users (37%) made use of their telecoil. Of a total of 363 aids dispensed, 26 (7%) were out of use. Experienced users differed significantly from first-time users: they had poorer hearing, they used their aids more than first-time users and they were more often treated binaurally.

Adolescent↗