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Retrospective study of morbidity and mortality of raptors admitted to Colorado State University Veterinary Teaching Hospital during 1995 to 1998.

A retrospective study was conducted to identify causes of morbidity and mortality of free-living raptors in northeast Colorado and the surrounding areas of Nebraska and Wyoming. The study included 409 raptors, representing 23 species, admitted to the Colorado State University Veterinary Teaching Hospital, Fort Collins, Colorado, USA, from 1995 to 1998. Causes of morbidity and mortality were identified as trauma (66.3%), orphaned young (15.6%), unknown (9.0%), infectious disease (4.4%), metabolic and nutritional disease (2.2%), toxicosis (2.0%), and degenerative disease (0.5%). Trauma was the most frequent cause of morbidity and mortality for all species and during all seasons.

Animals↗

Haemoproteus spp. and Leukocytozoon spp. in a captive raptor population.

Raptors are commonly infected with two blood parasites of the family Haemoproteidae, Haemoproteus spp. and Leukocytozoon spp. To determine if age or length of time in captivity influence prevalence of Haemoproteus spp. and Leukocytozoon spp. infection in captive raptors, blood samples were collected from 55 birds from April 1999 to May 2000. Blood smears were examined for parasitemia and influence of age and length of time in captivity at the time of sample collection were compared. We found juvenile and adult birds were more likely to be infected with Leukocytozoon spp. than were nestlings (P = 0.006) and birds present for > 365 days were more likely to be infected with Haemoproteus spp. and/or Leukocytozoon spp. than were birds captive for < 365 days.

Age Factors↗

Raptor responses to low-level jet aircraft and sonic booms.

We estimated effects of low-level military jet aircraft and mid- to high-altitude sonic booms (actual and simulated) on nesting peregrine falcons (Falco peregrinus) and seven other raptors by observing their responses to test stimuli, determining nesting success for the test year, and evaluating site reoccupancy rates for the year following the tests. Frequent and nearby jet aircraft passes: (1) sometimes noticeably alarmed birds, (2) occasionally caused birds to fly from perches or eyries, (3) most often evoked only minimal responses, and (4) were never associated with reproductive failure. Similarly, responses to real and simulated mid- to high-altitude sonic booms were often minimal and never appeared productivity limiting. Eighteen (95%) of 19 nest sites subjected to low-level jet flights and/or simulated sonic booms in 1980 fledged young during that year. Eighteen (95%) of 19 sites disturbed in 1980 were reoccupied by pairs or lone birds of the same species in 1981. We subjected four pairs of prairie falcons (Falco mexicanus) to low-level aircraft at ad libitum levels during the courtship and incubation phases when adults were most likely to abandon: all four eyries fledged young. From heart rate (HR) data taken via a telemetering egg at another prairie falcon eyrie, we determined that stimulus-induced HR alterations were comparable to rate changes for birds settling to incubate following flight. While encouraging, our findings cannot be taken as conclusive evidence that jet flights and/or sonic booms will have no long-term negative effects for other raptor species or for other areas. In addition, we did not experiment with totally naive wild adults, rotary-winged aircraft, or low-level sonic booms.

Journal Article↗

Hematocrit and blood chemistry values in captive raptors (Gyps fulvus, Buteo buteo, Milvus migrans, Aquila heliaca).

1. Eight hematological values were determined in blood samples from 88 raptors representing 10 species. 2. Means, standard errors, ranges and coefficients of variations were obtained for each parameter and species investigated. 3. The griffon vulture (Gyps fulvus) showed mean concentrations of glucose lower than those of most of the other species. 4. Concentrations of urea in the buzzard (Buteo buteo) were higher than those of the other raptors studied, and the black kite (Milvus migrans) had cholesterol values greater than the other species. 5. Blood values of individual birds of prey in good and bad states of nutrition were compared, showing significant changes in cholesterol, hematocrit, urea and total protein values.

Animals↗

Raptor protein contains a caspase-like domain.

Using state-of-the-art sequence analysis and structure-prediction methods a caspase-like domain in the N-terminal region of raptor proteins has been identified. This domain, which is characterized by the presence of invariant catalytic Cys-His dyad, is evolutionarily and structurally related to known caspases and might have protease activity. This finding suggests several unexpected aspects of raptor function in the target of rapamycin (TOR) signaling pathway.

Adaptor Proteins, Signal Transducing↗

Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor.

The performance of the fluorescent dye Alexa Fluor 647 (AF647) was explored as an alternative to Cy5 for immunoassays on the RAPTOR, a fiber optic biosensor. The RAPTOR performs sandwich fluoroimmunoassays on the surface of small polystyrene optical waveguides for analyte detection. Fluorescence and immunoassay data were examined at various dye-to-protein (D/P) ratios for both Cy5 and Alexa Fluor 647. Primarily, due to the self-quenching characteristics of Cy5, Alexa Fluor 647 is substantially more effective in fluoroimmunoassays, yielding over twice the signal for any given analyte concentration. Alexa Fluor 647 can be attached to antibodies at higher ratios, D/P=6, before self-quenching begins to limit the dye's effectiveness. Furthermore, while Alexa Fluor 647 becomes quenched at high dye-to-protein ratios, D/P=9, the net fluorescence yield reaches a maximum, as opposed to Cy5-labeled proteins, which become nearly nonfluorescent at high labeling ratios, D/P> or =6. The limitations of Cy5 were elucidated with an immunoassay for ricin, while the advantages of Alexa Fluor 647 were demonstrated in both direct binding assays as well as in a sandwich immunoassay for staphylococcal enterotoxin B.

Antibodies↗

Raptor: combining dual-shell representation, induced-fit simulation, and hydrophobicity scoring in receptor modeling: application toward the simulation of structurally diverse ligand sets.

We present a novel receptor-modeling approach (software Raptor) based on multidimensional quantitative structure-activity relationships (QSARs). To accurately predict relative free energies of ligand binding, it is of utmost importance to simulate induced fit. In Raptor, we explicitly and anisotropically allow for this phenomenon by a dual-shell representation of the receptor surrogate. In our concept, induced fit is not limited to steric aspects but includes the variation of the physicochemical fields along with it. The underlying scoring function for evaluating ligand-receptor interactions includes directional terms for hydrogen bonding and hydrophobicity and thereby treats solvation effects implicitly. This makes the approach independent from a partial-charge model and, as a consequence, allows one to smoothly model ligand molecules binding to the receptor with different net charges. We have applied the new concept toward the estimation of ligand-binding energies associated with the chemokine receptor-3 (50 ligands: r(2) = 0.965; p(2) = 0.932), the bradykinin B(2) receptor (52 ligands: r(2) = 0.949; p(2) = 0.859), and the estrogen receptor (116 ligands: r(2) = 0.908; p(2) = 0.907), respectively.

Binding Sites↗

Nuclear fuel-cycle radionuclides in raptor castings: implications for environmental monitoring.

Our sampling results indicate that raptor castings (regurgitated material containing the nondigestible remains of prey) faithfully reflect the composition of known environmental gamma-emitting radionuclides. The castings are easily obtainable and provide a way to monitor for the presence of biologically available radionuclides. Raptor castings can also provide a useful adjunct to existing monitoring programs where small mammal burrowing into buried waste may occur.

Animals↗

Ultraviolet reflectance by the cere of raptors.

Ultraviolet (UV) signals have been shown to play key roles in social and sexual signalling in birds. Using a spectrophotometer, we analysed the colour of the cere (skin above the beak) of a diurnal raptor, the Montagu's harrier (Circus pygargus), and show that it reflects in the UV part of the spectrum. The cere is a well-known sexual signal in raptors, with carotenoid based pigmentation being indicative of quality. We thus hypothesized that UV reflectance also signals quality. Accordingly, we found that in our sample of wild males, the location of the UV peak was related to the orangeness of cere and correlated with male body mass and condition (mass corrected for size). Also, males with brighter UV were mated to females that laid earlier, as expected if UV reflectance relates to a male's quality and attractiveness. Future studies should investigate the relationships between UV reflectance and carotenoid pigmentation of cere, and test how UV reflectance influences mate choice.

Animals↗

Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis.

The TSC1-TSC2/Rheb/Raptor-mTOR/S6K1 cell growth cassette has recently been shown to regulate cell autonomous insulin and insulin-like growth factor I (IGF-I) sensitivity by transducing a negative feedback signal that targets insulin receptor substrates 1 and 2 (IRS1 and -2). Using two cell culture models of the familial hamartoma syndrome, tuberous sclerosis, we show here that Raptor-mTOR and S6K1 are required for phosphorylation of IRS1 at a subset of serine residues frequently associated with insulin resistance, including S307, S312, S527, S616, and S636 (of human IRS1). Using loss- and gain-of-function S6K1 constructs, we demonstrate a requirement for the catalytic activity of S6K1 in both direct and indirect regulation of IRS1 serine phosphorylation. S6K1 phosphorylates IRS1 in vitro on multiple residues showing strong preference for RXRXXS/T over S/T,P sites. IRS1 is preferentially depleted from the high-speed pellet fraction in TSC1/2-deficient mouse embryo fibroblasts or in HEK293/293T cells overexpressing Rheb. These studies suggest that, through serine phosphorylation, Raptor-mTOR and S6K1 cell autonomously promote the depletion of IRS1 from specific intracellular pools in pathological states of insulin and IGF-I resistance and thus potentially in lesions associated with tuberous sclerosis.

Adaptor Proteins, Signal Transducing↗

A review of viruses affecting raptors.

Outbreaks of viral diseases have been diagnosed more commonly in raptors in recent years. The practice of feeding carnivorous birds with food derived from other birds exposes them directly to a wide range of potential pathogens. Some viruses which are avirulent in their natural host are known to be more pathogenic when they cross the species barrier. Compromised immunity due to stress or inbreeding may further increase the disease risk. Traditional feeding methods may need to be re-appraised and changed in view of this risk. This paper reviews the literature on viral diseases of raptors and provides additional clinicopathological observations from unpublished cases.

Animals↗

Hematozoa of raptors from southern New Jersey and adjacent areas.

Blood smears from 259 birds of 12 species, representing four families of raptors, from New Jersey, Pennsylvania, Delaware, and Virginia were examined for blood parasites. Infected birds constituted 59.1% of the total. Birds were infected with one or more of the following genera of protozoa: Leucocytozoon (43.2%); Haemoproteus (21.6%); Plasmodium (1.2%); and Trypanosoma (1.2%). Blood culture of 142 raptors of 11 species for Trypanosoma revealed a prevalence of 41.5%. Plasmodium circumflexum is reported for the first time in Accipiter striatus, and Trypanosoma sp. in Buteo jamaicensis.

Animals↗

Experimental infection of five species of raptors and of hooded crows with Francisella tularensis biovar palaearctica.

Sixteen raptors and three hooded crows were infected experimentally with Francisella tularensis biovar palaearctica. The birds were infected parenterally or per os. One goshawk, one sparrow hawk and one hooded crow died during the experimental period, and the remaining 16 birds were killed 14-77 days after the first infection. Francisella tularensis was not isolated from any bird. Antibody levels against F. tularensis measured in nine birds varied from 0 to 1:1,280. In one goshawk with a titer of 1:1,280, positive fluorescent antibody reactions against F. tularensis were seen in the liver and spleen. These results are similar to those found by other authors indicating that raptors and corvids are normally resistant to infections with F. tularensis.

Agglutination Tests↗

Antagonism of xylazine hydrochloride sedation in raptors by yohimbine hydrochloride.

The mean time to initial reversal response (MTIRR) and the mean time to perching (MTP) were measured in 34 raptors sedated with xylazine hydrochloride with dosages ranging from 1.0 to 20 mg/kg intravenously (i.v.) and 2.5 to 20.0 mg/kg intramuscularly (i.m.). Yohimbine hydrochloride, given i.v. (0.2 mg/kg), 30 min after the injection of the xylazine, shortened the MTIRR and MTP compared to the controls. No adverse effects were noted due to the use of yohimbine. Yohimbine appeared to be a safe and effective antagonist for xylazine sedation in raptors.

Animals↗

Embryonic and neonatal mortality from salmonellosis in captive bred raptors.

In a captive breeding center near Rome (Italy), cases of embryonic and neonatal death were recorded during the breeding seasons in the European eagle owl (Bubo bubo), peregrine falcon (Falco peregrinus), buzzard (Buteo buteo), and lanner falcon. (Falco biarmicus). Salmonella havana and S. virchow were isolated. Three pulli, clinically infected with S. havana, were successfully treated with enrofloxacin. From two groups of healthy 3- to 4-wk-old eagle owls, Salmonella sp. group 61 (61:r:-) and S. havana were collected. A strain of S. paratyphi B was detected in a pharyngeal swab and a fecal sample from an adult female goshawk (Accipiter gentilis), affected with pharyngeal trichomoniasis. A S. hadar strain was collected from a healthy 1-yr-old female eagle owl and S. livingstone was isolated from a 1-mo-old female peregrine, dead of an acute respiratory syndrome. Lesions of fibrinous polyserositis and multivisceral congestion were observed. From frozen 1-day-old chicks, on which adult and young raptors were fed, S. havana and S. livingstone isolates with similar biochemical and drug susceptibility patterns to those isolated from raptors were identified. A surveillance program on infectious diseases reduced embryonic and neonatal death rates in the following breeding seasons.

Animals↗

Lesions in the ocular posterior segment of raptors.

Thirty-eight free-living raptors, including various hawks, owls, and an eagle, had lesions of the ocular posterior segment believed to be the result of trauma. Although lesions of the anterior ocular segment and of the rest of the body often were seen, the lesions of the posterior segment of the eye were disproportionately more severe and usually were not suspected by rehabilitation personnel. The tightly encased raptor eye, with its anteriorly placed scleral ossicles, may have rendered the eye more susceptible to contrecoup damage. In the birds that were treated, ocular lesions were resolved and some vision was restored.

Animal Population Groups↗

Ocular lesions in free-living raptors.

In a retrospective study, records of 931 raptors admitted to the Avian Clinic at the New York State College of Veterinary Medicine and to the Owl Rehabilitation Research Foundation were evaluated to determine the prevalence, cause, and distribution of ocular lesions. Some form of ocular lesion was identified in 135 (14.5%) birds. Of these, 90% were the result of physical injury. Collisions accounted for 33% of ocular lesions; gunshot wounds accounted for 11%. Unilateral lesions were more common than bilateral lesions, with the anterior segment being most frequently involved. Hyphema was the most common clinical finding. In a prospective study involving raptors admitted to the Avian Clinic from 1980-1982, it was found that 17 of 61 birds (28%) had some form of ocular lesion. The higher prevalence in this study was attributed to a lower proportion of juvenile cases and to increased detection of subtle lesions, especially those involving the posterior segment.

Animals↗

Serotypes and DNA fingerprint profiles of Pasteurella multocida isolated from raptors.

Pasteurella multocida isolates from 21 raptors were examined by DNA fingerprint profile and serotyping methods. Isolates were obtained from noncaptive birds of prey found in 11 states from November 28, 1979, through February 10, 1993. Nine isolates were from bald eagles, and the remaining isolates were from hawks, falcons, and owls. Seven isolates were members of capsule group A, and 14 were nonencapsulated. One isolate was identified as somatic type 3, and another was type 3,4,7; both had unique HhaI DNA fingerprint profiles. Nineteen isolates expressed somatic type 1 antigen; HhaI profiles of all type 1 isolates were identical to each other and to the HhaI profile of the reference somatic type 1, strain X-73. The 19 type 1 isolates were differentiated by sequential digestion of DNA with HpaII; four HpaII fingerprint profiles were obtained. The HpaII profile of one isolate was identical to the HpaII profile of strain X-73. Incidence of P. multocida somatic type 1 in raptors suggests that this type may be prevalent in other wildlife or wildlife environments.

Animals↗