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[Experimental trial to reproduction enzootic haematuria in cattle by feeding bracken fern [Pteris aquilina (Kuhn)] (author's transl)].

Cattle were fed with a diet containing bracken fern in order to study the development of chronic bovine haematuria. The experimental 25 animals were randomly divided into 5 groups and such groups received different amounts of the plant. All groups were fed for 36 months, while no clinical effect of the disease was noted. After that time, the supplement of bracken fern to the diet was suspended and the animals still remained under observation. One of the animals (from group I) exhibit haematuria one month after the fern was removed from the diet. In the same occasion, four animals were slaughtered and only one of them presented haemorragic lesions in the urinary bladder. After 34 days another four animals were slaughtered and 114 days later three more were killed. All animals were chosen randomly and in spite of no clinical signs of the disease, all of them presented haemorragic lesions in the urinary bladder. The results obtained in this experiment confirmed that bracken fern (Pteris aquilina) is responsable for the development of chronic bovine haematuria in cattle. The disease is challenged by continuous ingestion of small amounts of bracken fern during a long period of time.

Animals↗

Tubular elements in plastids in the female gamete of a fern, Pteris ensiformis.

Highly dedifferentiated plastids in the egg cells of the fern Pteris ensiformis contain tubules with an external diameter of about 36 nm and an internal of 12 nm. The tubules are unlike those described from other eukaryotic plastids, but, although larger, have some resemblance to the rhapidosomes of blue-green algae.

Chloroplasts↗

Cell death induced by Pteris semipinnata L. is associated with p53 and oxidant stress in gastric cancer cells.

In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the mechanism of cytotoxicity. Classical features of apoptosis induced by 5F were observed in MKN-45 cells only or more significant in MKN-45 cells than MKN-28 cells. Translocation of Bax from cytosol to mitochondria, reduction of delta psi m and DNA fragmentation were induced by 5F in the p53-dependent manner. We conclude that the expression of Bax and its downstream molecules requires the presentation of a wild-type p53 in the cells treated by 5F.

Apoptosis↗